[Histonet] Automatic coverslippers

Hi Everyone, Just looking for opinions hereWhat automatic cover slipper do techs like best? And why? Thanks, Jessica Piche, HT(ASCP) Waterbury Hospital CONFIDENTIALITY NOTICE: This email and any attachments contain confidential information that is legally privileged. This information

[Histonet] FW: Freeze Spary Ban continued

Just checked ours: Richard Allan Scientific CYTOCOOL II has it as well!! Jessica Piche, HT(ASCP) Waterbury Hospital -Original Message- From: Macke, Gail via Histonet [mailto:histonet@lists.utsouthwestern.edu] Sent: Tuesday, March 01, 2016 1:13 PM To: Histology Cc:

[Histonet] Her2neu SP3 clone

Hi Everyone! Happy Friday! I was wondering if anyone would mind providing the protocol for your SP3 IHC stain on the Dako instruments you have? We are setting up an Omnis and would like a starting point for the assay... Thanks, Jessica Piche, HT(ASCP) CONFIDENTIALITY NOTICE: This email

[Histonet] Cryostat decontamination ANP23410

Hi Everyone, Just a quick question. If you defrost your cryostat to room temperature and wipe it out with 70% alcohol is that considered decontamination? Or does it have to be fumigated with formaldehyde, or have a UV function on board the machine? Thanks, Jessica Piche, HT(ASCP) Waterbury

[Histonet] CAP Gen 41770 glassware cleaning

Hi Everyone, I was wondering if anyone would mind sharing their procedure for glassware cleaning? We used to send our glassware down to Central Sterile supply but we have been bleaching our glassware/plastics and have had no issues with special stains. Is it a requirement to use detergent and

[Histonet] Harris Hematoxylin

Good Morning Everyone, Just wondering what kind of Harris Hematoxylin people are using. We are doing a regressive H on a Leica Autostainer XL for 9 minutes. We have been buying Ricca Hematoxylin but we are having so many issues with it. Are people using acidified Hematoxylin in regressive

[Histonet] Questions regarding microwave questions on Anatomic Pathology CAP Checklist

Happy New Year Everyone!! :) Questions on CAP Anatomic Path Checklist. Are the microwave questions referring to microwave processors/processing or the microwave oven that we use for special stains? Questions are ANP. 27170, 28290, 28860, and 29430. I'm thinking the microwave oven but I want

[Histonet] Xylene resistant gloves and xylene replacers

Good Morning Histonetters: I have two questions. Are there any gloves in existence (that are user friendly) that are resistant to xylene? And are there any xylene replacers out there that are actually safer than xylene? Thanks in advance and have a great day! Jessica Piche, HT (ASCP)

[Histonet] grossing credentials?

Good Morning, One more question. I was wondering if anyone is having a problem being credentialed for grossing with an Associate in Science in Veterinary Technology? Our state DPH is questioning my degree as not being a laboratory technology. I know there are lots of techs working in

[Histonet] PAX-8

Good Morning, Is anyone using PAX-8 from Biocare on the Dako Omnis and if so would you mind sharing your protocol/control tissue? I'll also take any information on any Pax8 protocols from different vendors/instruments. Thank you, Jessica Piche, HT(ASCP) Waterbury Hospital CONFIDENTIALITY

[Histonet] Microtomes

Good Morning Everyone, We are in the market for new microtomes and was wondering what everyone is using out there? We are looking for semi-automated/automated microtomes. We are demoing the Leica Autocut and the Thermo 355S. I really like the Leica microtome but am not crazy about the control

[Histonet] HSV 1&2 Control slides

Good Morning Everyone, I was wondering where everyone is getting control slides for HSV1&2 Antibody? I can't seem to find a lot of options. Thanks in advance! Jessica Piche, HT(ASCP) Waterbury Hospital ___ Histonet mailing list

[Histonet] bachelors of science

Hello Everyone, Do you need a bachelor's of science to sit for the HTL exam? I thought you had to have a bachelor's degree, and the right amount of credits in science, chemistry, etc. Thanks in advance. Jessica Piche, HT(ASCP) Waterbury Hospital ___

[Histonet] CAP Requirements for Scales and Balances

Good Afternoon All, Does anyone know if there are stated CAP requirements in regards to the calibration of scales and balances, and the intervals at which they should be calibrated? Thanks in advance and have a good day! Jessica Piche, HT (ASCP) Waterbury Hospital Waterbury, CT

[Histonet] pan-TRK or NTRK antibody

Good Afternoon, Where are people buying the pan-TRK or NTRK antibody from? Pre-dilute preferred. Thank you in advance and have a great day! Jessica Piche, HT(ASCP) Waterbury Hosptial Waterbury, CT 06708 ___ Histonet mailing list

[Histonet] Processing protocols

Good Morning Everyone, Our pathologist wants us to shorten our large tissue processing run so that we can run small biopsies with the bigger stuff when one of our tissue processors are down. We have 2 Excelsiors. Would anyone like to share any protocols they might have for this purpose? Thank

[Histonet] procedure manuals for special stains

Good Morning, I hope everyone is doing well. We have the Ventana Roche Benchmark special stainer. How is everyone doing their procedure manual for the actual stain protocols? What is the CAP requirement? Thank you, Jessica Jessica Piche, HT(ASCP) Waterbury Hospital Histology Laboratory

[Histonet] slide printers

Good Morning, Happy Friday Everyone. I was wondering what people are using/recommending for slide printers at the microtome? Do they all have to be connected to an LIS? Thank you, Jessica Jessica Piche, HT(ASCP) Histology Team Leader Waterbury Hospital Histology Laboratory

[Histonet] microtome blades and wax

Good Morning, We have been using Leica Histocore Autocut microtomes for a few months and have been having inconsistency with cutting on them. We have tried so many different blades, high and low, and have tried changing the blade angle. Nothing is a perfect fix, and we feel like we are really

[Histonet] Thyroid FNA's and other FNA procedures

Good Morning, Who makes the slides for fine needle aspirates at your facility? What are the qualifications to make the slides? I apologize if this message posted twice. Thank you, Jessica Jessica Piche, HT(ASCP) Waterbury Hospital Histology Laboratory Histology Team Leader 203-573-7167

[Histonet] Specimens for Gout

Good Morning Everyone, I hope you all had a nice weekend. What are people doing to process gout specimens? Are you fixing them in Absolute alcohol (and for how long), and then loading on tissue processor starting in xylene? I'm hearing different things and am curious about what other people

[Histonet] CAP regulatory question

Good Afternoon, Do we only have to keep lot to lot comparisons for immunohistochemistry and special stains for 2 years? I hope everyone is having a good day. Thank you. Jessica Jessica Piche, HT(ASCP) Waterbury Hospital Histology Laboratory Histology Team Leader 203-573-7167

Re: [Histonet] Tisse disposal/formalin

Hi Greg, I appreciate your response. Do you do this under a hood? If yes, is it vented outside? Thanks, Jessica Jessica Piche, HT(ASCP) Waterbury Hospital Histology Laboratory Histology Team Leader 203-573-7167 From: Greg Dobbin Sent: Wednesday, July 12,

[Histonet] Tissue disoposal/formalin

Good Afternoon, I have a question regarding the dumping of tissue. How are your laboratories dumping tissue when it is time for disposal? Are you putting the specimens with 10% neutral buffered formalin in the waste containers and a company comes and removes them from your facility? I would

Re: [Histonet] Coffee at the desk

It also doesn't set a good impression for everyone else in the lab since they can't have a coffee at their stations. If it could be done, I wouldn't want everyone else's coffees on my desk either, but I'd have to because I'd want to make it fair to everyone. If only it was safe for all of

[Histonet] SMAD4 antibody

Good Morning and Happy Friday! Is anyone running Smad4 on the Dako Omnis? If so, would you be willing to share your protocol? We are having a hard time working this one up. Thank you and have a great day! Jessica Piche, HT(ASCP) Jessica Piche, HT(ASCP) Waterbury Hospital Histology Laboratory

Re: [Histonet] Marking Pen

Hi Paula, What is the first solution the cassettes go in when the processor starts? We use Statmark pens when our cassette printer isn't working, and they work well. The only time we have had issues was when we would hand write the cassettes and run them on our small biopsy run which skips the

[Histonet] Premade Eosin

Good Afternoon, I hope everyone is having a great week so far! Can anyone share what pre-made eosin brands they are using and recommend? We use Eosin Y. We make it ourselves and want to look into pre-made. Thank you in advance. Have a great day. Jessica Jessica Piche, HT(ASCP) Waterbury

Re: [Histonet] preordering giemsa stains

Hi Paula, We do H.pylori on all specimens related to stomach upfront. We also do Ab Pas too. I think it is fairly common in lots of labs. Have a good day. Jessica Jessica Piche, HT(ASCP) Waterbury Hospital Histology Laboratory Histology Team Leader 203-573-7167

Re: [Histonet] Question Regarding HM355S Automatic Microtome

Hi Diana, We are not currently using the HM355S in our lab, but we did demo it and were not fans. I would see if you can demo one to see if you like it. We had the Thermo Shandon Finesse and Finesse Me models and we loved them but the HM355S didn't appear to be designed the same way and we