[Histonet] DAPI problem
Does anyone knows how to remove DAPI? I have some old sections with very deteriorated DAPI. I would like to remove it and stain again, do someone knows? Thank you! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RV: DAPI problem
And yes, I would like to remove the dapi residues without affecting the overall Alexa staining. Thank you! -Mensaje original- De: Alonso Martinez-Canabal [mailto:alonso.martinezcana...@utoronto.ca] Enviado el: September-10-12 5:32 PM Para: 'histonet@lists.utsouthwestern.edu' Asunto: DAPI problem Does anyone knows how to remove DAPI? I have some old sections with very deteriorated DAPI. I would like to remove it and stain again, do someone knows? Thank you! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Methyl green going away...
Hi my boss requires me to counterstain some DAB immunohistochemistry with methyl green. I use for that purpose the methyl green in aquos solution 0.5% in 4.2 acetate buffer. The problem is that after the washes I dehydrate and the methyl green becomes extremely faint with the alcohols. My protocol requires long periods of alcoholic dehydration very graded. Is there any idea on how to save the methyl green. Can I use an alcoholic solution? Thank you very much. Alonso ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Solvent-Resistant Ink
I would like to start to use some form of pen to permanent label my slides, the pencil marks somehow not very resistant to the immersion oil and that have created some problems. EM and other brands have some commercially available pens with solvent resistant ink. However, I am quite suspicious, how come the ink is resistant to Alcochol and Xylene, but you can remove with Alcohol/Xyline? So, do anyone has some success in the matter? Thank you very much. Alonso ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
