[Histonet] Part 4 - Book and Atlases - Final

[Connolly, Brett M via Histonet]

Hi all,

Thanks for the tremendous response! I am delighted that 38 books and atlases 
have found new homes. I only have 2 left if there is still interest in these.  
Sorry - no more freebies available.


Primate Brain Maps: Structure of the Macaque Brain;   ISBN 0-444-50415-X   
$50:00

Atlas of UroSurgical Anatomy;   ISBN 0-7216-3955-0  $30.00


Best regards,
Brett
Brett M. Connolly, Ph.D.
Prin. Scientist,
Translational Biomarkers - Imaging
Merck & Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_conno...@merck.com
T- 215-652-2501
F- 215-993-6803

Notice:  This e-mail message, together with any attachments, contains
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[Histonet] Part 3 - Book and Atlases they're going fast

[Connolly, Brett M via Histonet]

Hi all,

Thanks for all of your responses!  The list has shrunk considerably.

Here is what left.  I forgot to add the Sheehan & Hrapchak classic: Theory and 
Practice of Histotechnology (this one is a little worn---go figure).

Atlas of UroSurgical Anatomy;   ISBN 0-7216-3955-0  $30.00
Comparative Anatomy and Histology: A Mouse and Human atlas;   ISBN 
978-0-12-381361-9  $80.00
Human Histology 2nd Ed;   ISBN 0-7234-2485-3  $40.00
Immunochemistry (LabFax);  ISBN 1-872748-05-8   $12.00
Atlas of Normal Histology 6th Ed;   ISBN 0-8121-1126-5   $12.00
Primate Brain Maps: Structure of the Macaque Brain;   ISBN 0-444-50415-X   
$50:00
Structure of the Human Brain: A Photographic Atlas 3rd Ed:  ISBN 0-19-504357-X  
 $20.00
Theory and Practice of Histotechnology 2nd Ed;   ISBN 0-8016-4573-5  $50.00

Free books
A Textbook of Histology 10th Ed;   ISBN 0-7216-1757-3
The Cell: Its Organelles and Inclusions - Atlas of Fine Structure;  ISBN 
0-7216-3585-7
Ham' s Histology 9th Ed;  ISBN0-397-50681-3
Basic Histology 6th Ed:   ISBN 0-8385-0575-9
The Human Brain: An introduction to Its Functional Anatomy 5th Ed;  
ISBN0-323-01320-1
Animal Processing Manual 1st Ed; Nat. Soc for Histotechnology
Dictionary of Human Neuroanatomy   ISBN 3-540-66523-4



Brett M. Connolly, Ph.D.
Prin. Scientist,
Translational Biomarkers - Imaging
Merck & Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_conno...@merck.com
T- 215-652-2501
F- 215-993-6803

Notice:  This e-mail message, together with any attachments, contains
information of Merck & Co., Inc. (2000 Galloping Hill Road, Kenilworth,
New Jersey, USA 07033), and/or its affiliates Direct contact information
for affiliates is available at 
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proprietary copyrighted and/or legally privileged. It is intended solely
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[Histonet] Books and Atlases- Part 2 (with pricing and more books added)

[Connolly, Brett M via Histonet]

Hi all,

Thanks for all the responses to my previous post.  I really want to pass these 
along rather than trash them.

I updated the list and included my asking price. In addition I have a second 
list of free books - provided you purchase a book or books (one free book per 
purchase).

I'll pay for shipping within the continental US.

Couldn't be easierplease help me find a new home for these. I realize some 
are older, but histology and anatomy hasn't changed much.  You can Google the 
ISBN# or copy and paste the ISBN# into Amazon Books ISBN locator for more info 
on these.

I think they priced fairly, but I can negotiate.

Here is the more recent list:
Short Protocols in Molecular Biology 4thEd;   ISBN 0-471-32938-X  $25.00
Antigen Retrieval Techniques: Immunohistochemistry and Molecular Morphology;   
ISBN 1-881299-43-0 SOLD
Diagnostic Immunohistochemistry 2nd Ed;   ISBN 0-443-06652-3  SOLD
Atlas of Dermatopathology;   ISBN 0-7216-4886-X  $50.00
Atlas of UroSurgical Anatomy;   ISBN 0-7216-3955-0  $30.00
Comparative Anatomy and Histology: A Mouse and Human atlas;   ISBN 
978-0-12-381361-9  $80.00
Human Histology 2nd Ed;   ISBN 0-7234-2485-3  $40.00
A Color Atlas of Histology;ISBN 0-673-99190-3  $10.00
Color Atlas of Histology 3rd Ed;  ISBN 0-683-30642-1  $10.00
Histology for Pathologists 2nd Ed;ISBN 0-397-51718-1  SOLD
Immunochemistry (LabFax);  ISBN1-872748-05-8   $12.00
Immunological Reagents & Solutions: A Laboratory Handbook ;   ISBN 
1-881299-29-5   $15.00
Atlas of Normal Histology 6th Ed;   ISBN 0-8121-1126-5   $12.00
Histologic Preparations: Common Problems and Their Solutions;   ISBN 
978-0-930304-95-9  $50.00
Analytical Morphology: Theory, Applications & Protocols (soft cover);   ISBN 
1-881299-03-1   $15.00
A Color Atlas of Sectional Anatomy of the Mouse;   ISBN 4- 900659-58-4  $50.00
Gray's Anatomy 13th Ed;   ISBN 0-8121-0644-X$15.00
Primate Brain Maps: Structure of the Macaque Brain;   ISBN 0-444-50415-X   
$50:00
At Atlas of Primate Gross Anatomy: Baboon, Chimpanzee, and Man   ISBN  
0-89874-321-4  $50.00
Color Atlas of Nonhuman Primate Histology;   ISBN 3-8055-6879-7  $25.00
Structure of the Human Brain: A Photographic Atlas 3rd Ed:  ISBN 0-19-504357-X  
 $20.00
Atlas of the Rabbit Brain and Spinal Cord;  ISBN 3-8055-3814-6  $40.00
Laboratory Methods in Histotechnology (AFIP- red cover);   ISBN 1-881041-00-X  
$40.00

Here is the free book list:

A Textbook of Histology 10th Ed;   ISBN 0-7216-1757-3
Essential Immunology 6th Ed;   ISBN 0-632-01994-8
Antibody Applications: Essential Techniques;   ISBN 0-471-95698-8
Protein Localization by Fluorescence Microscopy: A Practical Approach;   ISBN 
0-19-963741-5
Techniques in Immunocytochemistry, Vol. 2;   ISBN 0-12-140405-6
Tissue In Situ Hybridization: Methods in Animal Development;   ISBN 
0-471-16403-8
The Cell: Its Organelles and Inclusions - Atlas of Fine Structure;  ISBN 
0-7216-3585-7
Ham' s Histology 9th Ed;  ISBN0-397-50681-3
Basic Histology 6th Ed:   ISBN 0-8385-0575-9
The Human Brain: An introduction to Its Functional Anatomy 5th Ed;  
ISBN0-323-01320-1
Gross Pathology: A Color Atlas;   ISBN 19-519797-6
Color Atlas of Histopathology;  ISBN 0-19-519151-X
Animal Processing Manual 1st Ed; Nat. Soc for Histotechnology
Dictionary of Human Neuroanatomy   ISBN 3-540-66523-4


Thanks,
Brett


Brett M. Connolly, Ph.D.
Prin. Scientist,
Translational Biomarkers - Imaging
Merck & Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_conno...@merck.com
T- 215-652-2501
F- 215-993-6803

Notice:  This e-mail message, together with any attachments, contains
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Re: [Histonet] JOH journal collection --it's claimed

[Connolly, Brett M via Histonet]

All gone.
Brett

Brett M. Connolly, Ph.D.
Prin. Scientist,
Translational Biomarkers - Imaging
Merck & Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_conno...@merck.com
T- 215-652-2501
F- 215-993-6803

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[Histonet] JOH journal collection

[Connolly, Brett M via Histonet]

HI all,

Is anyone interested in old issues of JOH? My collection spans  1997 - 2016. I 
am missing a few (~ 7).

Let me know and I'll send you a list of what I have.

Brett

Brett M. Connolly, Ph.D.
Prin. Scientist,
Translational Biomarkers - Imaging
Merck & Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_conno...@merck.com
T- 215-652-2501
F- 215-993-6803

Notice:  This e-mail message, together with any attachments, contains
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[Histonet] Thanks! CD3 antibody for mouse IHC

[Connolly, Brett M via Histonet]

Thanks to all who replied to my CD3 query! The responses were just what I was 
hoping for.

Best regards,
Brett

Brett M. Connolly, Ph.D.
Prin. Scientist,
Translational Biomarkers - Imaging
Merck & Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_conno...@merck.com
T- 215-652-2501
F- 215-993-6803

Notice:  This e-mail message, together with any attachments, contains
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[Histonet] CD3 antibody for FFPE mouse tissue

[Connolly, Brett M via Histonet]

Hi all,

Looking for recommendation for an anti-mouse CD3 antibody for FFPE sections.

I have some old Histonet messages referring to a Neomarkers RB-360 ab which I 
found through Thermo, but I would like to hear about more recent 
experiences/recommendations

Thanks as always,
Brett

Brett M. Connolly, Ph.D.
Prin. Scientist,
Translational Biomarkers - Imaging
Merck & Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_conno...@merck.com
T- 215-652-2501
F- 215-993-6803

Notice:  This e-mail message, together with any attachments, contains
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[Histonet] HSV-TK antibody for IHC

[Connolly, Brett M via Histonet]

Anyone know of an anti-HSV tyrosine kinase antibody suitable for IHC?  The ones 
I am finding are WB and ELISA only.

Thanks,
Brett

Brett M. Connolly, Ph.D.
Prin. Scientist,
Translational Biomarkers - Imaging
Merck & Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_conno...@merck.com
T- 215-652-2501
F- 215-993-6803

Notice:  This e-mail message, together with any attachments, contains
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Re: [Histonet] HT Exam

[Connolly, Brett M via Histonet]

For $30.00 you can get them from ASCPprobably a good investment !!

http://www.starttest.com/7.2.0.0/cart.aspx?program=ASCPPractice

Brett

Brett M. Connolly, Ph.D.
Prin. Scientist, 
Translational Biomarkers - Imaging
Merck & Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_conno...@merck.com
T- 215-652-2501
F- 215-993-6803




-Original Message-
From: Kristy Castillo via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Tuesday, March 29, 2016 8:41 AM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] HT Exam

How would I be able to get a example test for the HT exam?  Is there a place 
that you can go to get example questions?  Know someone studying for the exam, 
but she is not sure what to study and is really nervous.   Thanks Histonetters!

Kristy Castillo


This transmission may contain confidential information, some or all of which 
may be protected health information as defined by the federal Health Insurance 
Portability & Accountability Act (HIPAA) Privacy Rule. This transmission is 
intended for the exclusive use of the individual or entity to whom it is 
addressed and may contain information that is proprietary, privileged, 
confidential and/or exempt from disclosure under applicable law. If you are not 
the intended recipient (or an employee or agent responsible for delivering this 
transmission to the intended recipient), you are hereby notified that any 
disclosure, dissemination, distribution or copying of this information is 
strictly prohibited and may be subject to legal restriction or sanction. Please 
notify the sender by telephone to arrange the return or destruction of the 
information and all copies.
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[Histonet] Masson's Trichrome or Sirius Red on frozen tissue

[Connolly, Brett M via Histonet]

Has anyone preformed these stains of frozen sections??? Looking for any tips.

I'll have fresh frozen tissue that can be fixed after sectioning and mounting 
onto the slide.

Thanks,
Brett

Brett M. Connolly, Ph.D.
Prin. Scientist,
Translational Biomarkers - Imaging
Merck & Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_conno...@merck.com
T- 215-652-2501
F- 215-993-6803

Notice:  This e-mail message, together with any attachments, contains
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Re: [Histonet] Lab Coats and OCT

[Connolly, Brett M via Histonet]

I am a fan of Cryo-Gel - highly viscous which facilitates easier tissue 
orientation. We get it from Electron Microscopy Sciences (#62806-01).
Try it, you'll like it.

Brett

Brett M. Connolly, Ph.D.
Prin. Scientist, 
Translational Biomarkers - Imaging
Merck & Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_conno...@merck.com
T- 215-652-2501
F- 215-993-6803



-Original Message-
From: Seeley, Heather via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Thursday, February 18, 2016 11:16 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Lab Coats and OCT

Hello All,





Now that the hospital is enforcing us to wear lab coats all the time, wondering 
if anyone has suggestions for some that aren't too hot, the ones we have now 
are making us sweat!



Also, we have been having issues with our OCT, we currently use the Sakura OCT 
and have use it for years. Within the last 6 months or so it has become like 
glue on the chucks even if we let it soak in hot water and scrub with a brush, 
it just doesn't want to come out! If anyone has any suggestions on brands to  
try we would appreciate it!

Thanks!


HEATHER SEELEY, HT(ASCP)
Histotechnologist
803-985-4676 OFFICE
803-327-7598 FAX

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[Histonet] PDGFR beta antibody for IHC

[Connolly, Brett M via Histonet]

Anyone have a favorite for rodent FFPE tissue?

Thanks,
Brett

Brett M. Connolly, Ph.D.
Prin. Scientist,
Translational Biomarkers - Imaging
Merck & Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_conno...@merck.com
T- 215-652-2501
F- 215-993-6803

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Re: [Histonet] DIF on paraffin embedded tissue

[Connolly, Brett M via Histonet]

Also agree. Lots of people do it. Search PubMed, J. Histo Cyto.

If you have the appropriate filters, using Cy dye secondary's can help move 
away from any autofluorescence.

Brett

Brett M. Connolly, Ph.D.
Prin. Scientist, 
Translational Biomarkers - Imaging
Merck & Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_conno...@merck.com
T- 215-652-2501
F- 215-993-6803




-Original Message-
From: Marcus Green via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Wednesday, November 25, 2015 12:23 PM
To: Elizabeth Chlipala; Simmons, Christopher; Rene J Buesa
Cc: 'histonet@lists.utsouthwestern.edu' (histonet@lists.utsouthwestern.edu)
Subject: Re: [Histonet] DIF on paraffin embedded tissue


I, agreeing with Liz,

would suggest looking out a IF protocol for FFPE tissues - I've recently 
concluded a validation of PLA (proximity ligation assay) in: cells in culture, 
FFPE cell pellets and FFPE xenografts. The signal was consistently there 
(although a drop in expression - from fixative reasons as well as culture 
conditions). Background is the biggest issue for IF using FFPE so make sure to 
run some comprehensive controls.

You will need to run a Deparafinisation, Dehydration and Rehydration as you 
would IHC (3mins xylene x2, 3mins 100% EtOH, 3mins 70%EtOH and 3mins 50%EtOH - 
or similar) before running an Antigen Retrieval - if you have this optimised 
for IHC use something similar (pH and time). I would also consider a 
permeabilization step and if you need to optimise for background (Sudan black 
wash).

If, however the question is can you reinstate a tissue back to an unfixed 
tissue for freeze processing then no you can't.

hope this helps,
kind regards,

Marcus


From: Elizabeth Chlipala via Histonet [histonet@lists.utsouthwestern.edu]
Sent: 25 November 2015 16:53
To: Simmons, Christopher; Rene J Buesa
Cc: 'histonet@lists.utsouthwestern.edu' (histonet@lists.utsouthwestern.edu)
Subject: Re: [Histonet] DIF on paraffin embedded tissue

I'm not sure that is the case in the grand scheme of things, it will depend 
upon the target that you need to stain via immunofluorescence.  Technically we 
perform IF staining on frozen sections primarily because the antigen does not 
survive formalin fixation.  Many people utilize IF techniques on FFPE tissue 
with good success, we have done it here.  There are things you will need to 
consider and what I suggest below may not work at all.  It's up to you if you 
want to try it or not and you may feel it is not worth the time and energy 
required to see if it may work on FFPE samples, it could be a lot of work and 
it may not be successful.

1.  You will likely not be able to use your current protocol
2.  You will likely need to add an antigen retrieval step
3.  You may need to look for a different antibody source (one that survives 
formalin fixation)
4.  The signal needs to be good since formalin fixation will cause 
autofluorescence

Unless I am completely missing something here since two individuals have stated 
that the sample is useless, maybe there is a better explanation as to why the 
sample is completely useless.  Here is where I am coming from - technically you 
can perform IF staining on FFPE tissue samples, people do it all of the time, 
we have done it here, it's a common technique for dual labeling of samples when 
co-localization is an expected result.

Good Luck

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Laboratory, LLC
PO Box 18592
Boulder, CO 80308
(303) 682-3949 office
(303) 682-9060 fax
(303) 881-0763 cell
l...@premierlab.com
www.premierlab.com

Ship to Address:

Premier Laboratory, LLC
1567 Skyway Drive, Unit E
Longmont, CO 80504

-Original Message-
From: Simmons, Christopher via Histonet 
[mailto:histonet@lists.utsouthwestern.edu]
Sent: Wednesday, November 25, 2015 9:10 AM
To: Rene J Buesa
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] DIF on paraffin embedded tissue

Useless sample
Sorry

Sent from my iPhone

> On Nov 25, 2015, at 11:02 AM, Rene J Buesa via Histonet 
>  wrote:
>
> No matter WHO to tell you to do WHAT, for IF purposes, that FFPE
> tissue is USELESS.René
>
>
>On Wednesday, November 25, 2015 10:55 AM, Maryann Deathridge via Histonet 
>  wrote:
>
>
> We have a tissue sample that was processed and paraffin embedded.  We
> URGENTLY need to recover the tissue and perform Immunofluorescence on
> the sample.
> Does anyone have a procedure.  HELP
>
> madeathri...@pastnashville.com
>
>
>
>
>
>
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[Histonet] CD8 IHC on FFPE mouse tissue -what your experience?

[Connolly, Brett M via Histonet]

Hi all,
I have a project that will be looking at CD8 expression in some FFPE murine 
tissue.  Past discussions here have mentioned the 14-0808 CD8a antibody from 
eBiosicence.

For those of you have had the opportunity to use this antibody I would like to 
hear your experiences/ recommendations about the staining results.

I'll need to do some quantitation of the staining so I am hoping this antibody 
is strong and specific.

Thanks much,
Brett

Brett M. Connolly, Ph.D.
Prin. Scientist,
Translational Biomarkers - Imaging
Merck & Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_conno...@merck.com
T- 215-652-2501
F- 215-993-6803

Notice:  This e-mail message, together with any attachments, contains
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Re: [Histonet] autoradiography frozen human brain sections fall off, white matter issue

[Connolly, Brett M via Histonet]

Hi Esther,

We do a lot of autoradiography on rodent, primate and human brains sections. 
Our protocol is similar to yours except we do not dry the slides after 
incubating in the buffer and before adding the radiotracer.

The experiment is performed with the slides in a rack and totally immersed in 
the solutions in staining dishes.

1. incubate slides in assay buffer 15 min.  at RT
2. add radiotracer to buffer, gently mix and incubate 90-120 min. at RT
3. wash  GENTLY (no agitation) in ice-cold wash buffer 3 x 3 min. We set up 3 
containers and transfer the slides.
4. wash in ice-cold DI water 5 seconds.
5. air dry

The Superfrost gold slides are supposed to improve adherence and would be worth 
a try. It could be your other Superfrost slides are old or a bad batch...which 
I think has been experienced by some people on the list.

We do store our sectioned slides at -70C until use and then bring them up to RT 
the day of the experiment. They are sectioned, dried at RT for 15-20 min, and 
then store at -70C... No drying in the fridge.

Good luck,
Brett


Brett M. Connolly, Ph.D.
Principle Scientist, 
Translational Biomarkers - Imaging
Merck & Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_conno...@merck.com
T- 215-652-2501
F- 215-993-6803

-Original Message-
From: Kooijman, Esther via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Friday, October 02, 2015 5:20 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] autoradiography frozen human brain sections fall off, white 
matter issue
Importance: High

Dear Histonetters,

Does anyone of you have experience in autoradiography and could help me with 
the problem falling of tissue  ? Or suggestions ?

We have problems with falling off sections of human brain tissue.
We have done the same experiments and cutting in rodent tissue without any 
problems. Our protocol for autoradiography  :
Snap frozen tissue, cut in cryo 20um sections on Superfrost plus glass, drying 
ON in the fridge on silica.
Stored in -20.
Day of the experiment.
Getting them on room temperature about 45 minutes, washing/dehydrate on room 
temperature in 5mM Tris (HCL PH7.4) buffer, 20 minutes.
About  30 minutes - an hour drying at room temperature.
Then incubation with the radio tracer for 30 minutes, room temperature, just 
dripping the 1 mL solution to completely cover the superfrost plus glass and 
ditto tissue.
Turning the glass to get the solution of the glass,  dipping in ice cold tris 
washing buffer 5mM (HCL PH7.4) 1.5 minutes and here the disaster starts with 
falling off extensive white matter falling off.. its washing steps of only 
1.5 minute.

What can I do to prevent this, any idea ? I am out of clues. I have tried to 
dry the sections longer after cutting sections, I have tried to pre wash in 
either room temp or cold buffer. Hope someone can help me.
Would Superfrost plus gold glass be better ?

Kind Regards,

Esther Kooijman
Research Technician
VU University Medical Center
Nuclear Medicine & PET Research
Email: e.kooij...@vumc.nl
Amsterdam
The Netherlands

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Re: [Histonet] Tissue request....

[Connolly, Brett M via Histonet]

Rachel,

If you have funding to purchase you can check out these biorepositories...

Asterand Bioscience
http://www.asterandbio.com/

National Disease Research Interchange
http://ndriresource.org/

Cooperative Human Tissue Network
http://www.chtn.nci.nih.gov/

Analytical Biological Services
http://www.absbio.com/

Cureline Laboratories
http://www.cureline.com/


Brett M. Connolly, Ph.D.
Principle Scientist, Imaging Dept.
Translational Biomarkers
Merck & Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_conno...@merck.com
T- 215-652-2501
F- 215-993-6803





From: Rachel M Gonzalez via Histonet [histonet@lists.utsouthwestern.edu]
Sent: Friday, September 18, 2015 7:19 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Tissue request

Hi

Looking for control tissue for reviewing antibody specificity this is for
R project. I do not know if that will make a difference in the tissue
availability but I thought I put that out there.  If you know a good tissue
source can you let me know.

I need three of each type

normal bone marrow (hematopoietic cell and lymphocytes, and etc.),

normal brain/cerebrum,

normal cerebellum,

normal parathyroid glands,

normal hypophysis/Pituitary Gland

normal thymus.



Thank you in advance for help.

Rachel Gonzalez PhD

Senior Scientist
.
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