[Histonet] unsubscribe
I would like to unsubscribe to this list Regards Daniela Bodemer Research Assistant Surgical Research, Infection Immunity Murdoch Childrens Research Institute The Royal Children's Hospital Flemington Road Parkville Victoria 3052 Australia E daniela.bode...@mcri.edu.aumailto:daniela.bode...@mcri.edu.au www.mcri.edu.auhttp://www.mcri.edu.au/ Celebrating 25 Years of Life Saving Discoveries This e-mail and any attachments to it (the Communication) are, unless otherwise stated, confidential, may contain copyright material and is for the use only of the intended recipient. If you receive the Communication in error, please notify the sender immediately by return e-mail, delete the Communication and the return e-mail, and do not read, copy, retransmit or otherwise deal with it. Any views expressed in the Communication are those of the individual sender only, unless expressly stated to be those of Murdoch Childrens Research Institute (MCRI) ABN 21 006 566 972 or any of its related entities. MCRI does not accept liability in connection with the integrity of or errors in the Communication, computer virus, data corruption, interference or delay arising from or in respect of the Communication. P Please consider the environment before printing this email __ This email has been scanned by the Symantec Email Security.cloud service. For more information please visit http://www.symanteccloud.com __ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Fixation time
Hi all, Our tissue processor has been shut down due to a contamination issue and now all the tissues (mice and rat pelves) collected prior to this happening have been sitting in 4% PFA. Some tissues more than a week, when we usually fix for 48 hours. Now we are transferring the tissues to 70% Ethanol and they will sit there until further notice. I am concerned about this process and what it will do to the tissue and would like your thoughts on this. Many thanks, DB Research Assistant __ This email has been scanned by the Symantec Email Security.cloud service. For more information please visit http://www.symanteccloud.com __ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Frozens and antigen retrieval
Hi all, A question from my student: antigen retrieval when using frozens for immunofluorescence -yes or no? The protocol suggested by the antibody company lists the retrieval as an option. I am used to do retrieval on paraffin sections, but not on cryo sections. Hit me with your opinions on this :-) Thanks in advance, Daniela Sent from my iPad __ This email has been scanned by the Symantec Email Security.cloud service. For more information please visit http://www.symanteccloud.com __ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Macrophage stain
Hi all, I would like to stain for macrophages in mice aorta (5um frozen sections). Would you have suggestions on which stains to use and perhaps share the protocol? Thanks in advance, Daniela Bodemer Research Assistant Surgical Research, Infection and Immunity Murdoch Childrens Research Institute The Royal Children's Hospital Flemington Road Parkville Victoria 3052 Australia T 03 9936 6676 T (03 9936 6794) E daniela.bode...@mcri.edu.au mailto:firstname.surn...@mcri.edu.au www.mcri.edu.au http://www.mcri.edu.au/ This e-mail and any attachments to it (the Communication) are, unless otherwise stated, confidential, may contain copyright material and is for the use only of the intended recipient. If you receive the Communication in error, please notify the sender immediately by return e-mail, delete the Communication and the return e-mail, and do not read, copy, retransmit or otherwise deal with it. Any views expressed in the Communication are those of the individual sender only, unless expressly stated to be those of Murdoch Childrens Research Institute (MCRI) ABN 21 006 566 972 or any of its related entities. MCRI does not accept liability in connection with the integrity of or errors in the Communication, computer virus, data corruption, interference or delay arising from or in respect of the Communication. P Please consider the environment before printing this email __ This email has been scanned by the Symantec Email Security.cloud service. For more information please visit http://www.symanteccloud.com __ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] FW: Oil Red O
From: Daniela Bodemer Sent: Thursday, 16 February 2012 9:59 AM To: Histonet@lists.utsouthwestern.edu Subject: Oil Red O Hi all, I am staining cryo sections of aorta for lipids with Oil Red O and mounting my slides with Mowiol. My question is how long will they last without fading and should they be kept in a folder in the fridge? Thanks for your thoughts, Daniela Daniela Bodemer Research Assistant Surgical Research, Infection and Immunity Murdoch Childrens Research Institute The Royal Children's Hospital Flemington Road Parkville Victoria 3052 Australia T 03 9936 6676 T (03 9936 6794) E daniela.bode...@mcri.edu.au mailto:firstname.surn...@mcri.edu.au www.mcri.edu.au http://www.mcri.edu.au/ This e-mail and any attachments to it (the Communication) are, unless otherwise stated, confidential, may contain copyright material and is for the use only of the intended recipient. If you receive the Communication in error, please notify the sender immediately by return e-mail, delete the Communication and the return e-mail, and do not read, copy, retransmit or otherwise deal with it. Any views expressed in the Communication are those of the individual sender only, unless expressly stated to be those of Murdoch Childrens Research Institute (MCRI) ABN 21 006 566 972 or any of its related entities. MCRI does not accept liability in connection with the integrity of or errors in the Communication, computer virus, data corruption, interference or delay arising from or in respect of the Communication. P Please consider the environment before printing this email __ This email has been scanned by the Symantec Email Security.cloud service. For more information please visit http://www.symanteccloud.com __ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Oil Red O
Hi all, I am staining cryo sections of aorta for lipids with Oil Red O and mounting my slides with Mowiol. My question is how long will they last without fading and should they be kept in a folder in the fridge? Thanks for your thoughts, Daniela Daniela Bodemer Research Assistant Surgical Research, Infection and Immunity Murdoch Childrens Research Institute The Royal Children's Hospital Flemington Road Parkville Victoria 3052 Australia T 03 9936 6676 T (03 9936 6794) E daniela.bode...@mcri.edu.au mailto:firstname.surn...@mcri.edu.au www.mcri.edu.au http://www.mcri.edu.au/ This e-mail and any attachments to it (the Communication) are, unless otherwise stated, confidential, may contain copyright material and is for the use only of the intended recipient. If you receive the Communication in error, please notify the sender immediately by return e-mail, delete the Communication and the return e-mail, and do not read, copy, retransmit or otherwise deal with it. Any views expressed in the Communication are those of the individual sender only, unless expressly stated to be those of Murdoch Childrens Research Institute (MCRI) ABN 21 006 566 972 or any of its related entities. MCRI does not accept liability in connection with the integrity of or errors in the Communication, computer virus, data corruption, interference or delay arising from or in respect of the Communication. P Please consider the environment before printing this email __ This email has been scanned by the Symantec Email Security.cloud service. For more information please visit http://www.symanteccloud.com __ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Immunofluorescence
Hi Natalia, Human tissue is naturally autofluorescent. In our lab we do immunos on frozens and quench the AF with chicago blue solution (1g Chicago Sky Blue 6B-Sigma in 99ml MilliQ plus 1ml DMSO) after washing the secondary antibodies with PBS (3x). Just load the tissue with a drop of the CB solution for 15 secs and wash it in PBS again (3x). Mount as usual D -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Natalia Fernandez Sent: Wednesday, 8 February 2012 2:35 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Immunofluorescence Hi everybody! I have a problem with my experiment. I try to do immunofuorescences in old human brains (parafine sections). First I thought that the too much colocalisation was due to my antibodies (primary or secondary). After several tests (Only 1st antibody, only 2nd one, simple staining, inverse simple staining,..) I saw that the tissue itself shows a lot of fluorescence (without antibodies, and even after a treatment against lipofuscine (solution of Potassium permanganate). So my question is: Do you have the same problem? Do you think that parafine could be the reason of this autofluorescence?! What can I do? Thank you so much for your answers :) Natalia ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __ This email has been scanned by the Symantec Email Security.cloud service. For more information please visit http://www.symanteccloud.com If you have any question, please contact MCRI IT Helpdesk for further assistance. __ __ This email has been scanned by the Symantec Email Security.cloud service. For more information please visit http://www.symanteccloud.com __ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Quenching auto fluorescence in human tissue
Hi all, I have been experiencing trouble with my secondary antibody when doing my immuno with human colon tissue. I use to use FITC 488 as my secondary antibody and changed over to Alexa488 due to the FITC's manufacturing stop. The auto fluorescence of the human tissue is quenched with Chicago Blue solution and the slides are mounted with Prolong Gold antifade reagent to extend the life of the immuno slides. The trouble is, after a few days the fluorofors seem to 'detach' and start floating around, not allowing reliable validation of the results. This also happen if I use Mowiol as a mounting medium. Is the mounting medium reacting with Chicago Blue? Is there any alternative to quench die auto fluorescence of the tissue? Thanks for your ideas. Daniela Bodemer Research Assistant Surgical Research, Infection and Immunity Murdoch Childrens Research Institute The Royal Children's Hospital Flemington Road Parkville Victoria 3052 Australia T 03 9936 6676 T (03 9936 6794) E daniela.bode...@mcri.edu.au mailto:firstname.surn...@mcri.edu.au www.mcri.edu.au http://www.mcri.edu.au/ This e-mail and any attachments to it (the Communication) are, unless otherwise stated, confidential, may contain copyright material and is for the use only of the intended recipient. If you receive the Communication in error, please notify the sender immediately by return e-mail, delete the Communication and the return e-mail, and do not read, copy, retransmit or otherwise deal with it. Any views expressed in the Communication are those of the individual sender only, unless expressly stated to be those of Murdoch Childrens Research Institute (MCRI) ABN 21 006 566 972 or any of its related entities. MCRI does not accept liability in connection with the integrity of or errors in the Communication, computer virus, data corruption, interference or delay arising from or in respect of the Communication. P Please consider the environment before printing this email __ This email has been scanned by the Symantec Email Security.cloud service. For more information please visit http://www.symanteccloud.com __ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] gelatine, silane, super frost plus slides ???
Hi all, I am putting information together for students and thought you might be able to help me. Gelatine, silane, super frost plus slides. What are the differences and characteristics and which slide to use for what? Thank you, Daniela Bodemer __ This email has been scanned by the Symantec Email Security.cloud service. For more information please visit http://www.symanteccloud.com __ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
FW: [Histonet] Chicago Sky Blue stain vs. KOH - striking comparison
We use Chicago Sky Blue 6B from Sigma C-8679 (2610-05-1). We make our solution as followed: 1g CSB 6B 1ml DiMethylsulphoxid 99ml Dist water Daniela Bodemer Research Assistant Surgical Research, Infection and Immunity Murdoch Childrens Research Institute The Royal Children's Hospital Flemington Road Parkville Victoria 3052 Australia T 03 9345 5930 T (03 9345 4116) E daniela.bode...@mcri.edu.au www.mcri.edu.au -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Harrison, Sandra C. Sent: Friday, 11 November 2011 4:46 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Chicago Sky Blue stain vs. KOH - striking comparison Does anyone have ordering information for a stain called Chicago Sky Blue(CSB)? I just saw an article in the NOV issue of ASCP LABMEDICINE (page 649) comparing KOH vs. CSB and, wow, is that a beautiful stain. The results were dramatic, with about a 25-50% increase in the Dermatophytes identified and about a 5X increase in the Pityriasis versicolor identification. Thanks, Sandy C. Harrison, HTL (ASCP) Histology Supervisor Minneapolis VA 612-467-2449 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email If you have any question, please contact MCRI IT Helpdesk for further assistance. __ __ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email __ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Problems with Frozen tissues
Our tissues are frozen in OCT. I use Super Frost Plus slides from Thermo Scientific and store the sections in the fridge until I need them. After leaving them out on the bench for an hour I start my HE with 1 min running water and so on. I rarely lose sections with these slides. Daniela -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kim Donadio Sent: Tuesday, 1 November 2011 3:02 AM To: Igor Deyneko; Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Problems with Frozen tissues Ive never just air dried my frozen sections. always put them in a fixative such as pen fix, a alcohol and or formalin mixture, something( depends on what your going to look for, test etc). That with using charged slides and never had too many problems with this. Kim From: Igor Deyneko igor.deyn...@gmail.com To: Histonet@lists.utsouthwestern.edu Sent: Monday, October 31, 2011 9:56 AM Subject: [Histonet] Problems with Frozen tissues I'm looking for some advice on frozen tissues. This is the first time I'm doing it. All the tissues: skin, lungs, spleen, liver, and pancreas cut well onto special Gold Plus slides from Fisher. Then, when I was ready to stain the slides, i air dried them fro an hour and wanted to do HE and Beta-Gal, all the tissues fell off slides. Can anyone suggest any tips on preventing this mischief? Thank you in advance. Igor Deyneko Infinity Pharmaceuticals Cambridge, MA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email If you have any question, please contact MCRI IT Helpdesk for further assistance. __ __ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email __ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] DAB Immuno and alcian blue counter staining
Hi all, Is it possible to do an immuno with DAB detection and counterstain it with alcian blue at the end to detect Goblets cells? I've tried it and the DAB disappears under the staining. Thanks, Daniela __ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email __ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] processor stopped - help!
Hi all, When I arrived the lab this morning I found the processor basket with my cassettes stuck between the two paraffin tanks. I suppose it went through the first cycle but not the second one, so its missing 1.5 hrs of paraffin infusion. Should I put them back in hot paraffin for 1.5 hours or what can I do to save the samples? Thanks for your advice, Daniela Bodemer Research Assistant Surgical Research, Infection and Immunity Murdoch Childrens Research Institute The Royal Children's Hospital Flemington Road Parkville Victoria 3052 Australia T 03 9345 5930 T (03 9345 4116) E daniela.bode...@mcri.edu.au mailto:firstname.surn...@mcri.edu.au www.mcri.edu.au http://www.mcri.edu.au/ This e-mail and any attachments to it (the Communication) are, unless otherwise stated, confidential, may contain copyright material and is for the use only of the intended recipient. If you receive the Communication in error, please notify the sender immediately by return e-mail, delete the Communication and the return e-mail, and do not read, copy, retransmit or otherwise deal with it. Any views expressed in the Communication are those of the individual sender only, unless expressly stated to be those of Murdoch Childrens Research Institute (MCRI) ABN 21 006 566 972 or any of its related entities. MCRI does not accept liability in connection with the integrity of or errors in the Communication, computer virus, data corruption, interference or delay arising from or in respect of the Communication. P Please consider the environment before printing this email __ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email __ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] processor stopped - help!
I am glad to hear the samples will be fine. Thanks everyone! Daniela Bodemer Research Assistant Surgical Research, Infection and Immunity Murdoch Childrens Research Institute The Royal Children's Hospital Flemington Road Parkville Victoria 3052 Australia T 03 9345 5930 T (03 9345 4116) E daniela.bode...@mcri.edu.au mailto:firstname.surn...@mcri.edu.au www.mcri.edu.au http://www.mcri.edu.au/ This e-mail and any attachments to it (the Communication) are, unless otherwise stated, confidential, may contain copyright material and is for the use only of the intended recipient. If you receive the Communication in error, please notify the sender immediately by return e-mail, delete the Communication and the return e-mail, and do not read, copy, retransmit or otherwise deal with it. Any views expressed in the Communication are those of the individual sender only, unless expressly stated to be those of Murdoch Childrens Research Institute (MCRI) ABN 21 006 566 972 or any of its related entities. MCRI does not accept liability in connection with the integrity of or errors in the Communication, computer virus, data corruption, interference or delay arising from or in respect of the Communication. P Please consider the environment before printing this email __ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email __ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Reorienting frozen tissue
Hi all, I would like to know if it is possible and how to reorient or reembedd human tissue, that has been frozen at -80C in OCT. Many thanks, Daniela Bodemer Research Assistant Surgical Research, Infection and Immunity Murdoch Childrens Research Institute The Royal Children's Hospital Flemington Road Parkville Victoria 3052 Australia T 03 9345 5930 T (03 9345 4116) E daniela.bode...@mcri.edu.au mailto:firstname.surn...@mcri.edu.au www.mcri.edu.au http://www.mcri.edu.au/ This e-mail and any attachments to it (the Communication) are, unless otherwise stated, confidential, may contain copyright material and is for the use only of the intended recipient. If you receive the Communication in error, please notify the sender immediately by return e-mail, delete the Communication and the return e-mail, and do not read, copy, retransmit or otherwise deal with it. Any views expressed in the Communication are those of the individual sender only, unless expressly stated to be those of Murdoch Childrens Research Institute (MCRI) ABN 21 006 566 972 or any of its related entities. MCRI does not accept liability in connection with the integrity of or errors in the Communication, computer virus, data corruption, interference or delay arising from or in respect of the Communication. P Please consider the environment before printing this email __ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email __ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
