Re: [Histonet] Type of Nitrile Gloves in your lab

[Haley Huggins via Histonet]

I like the Halyard brand.  We also use Confiderm from McKesson, those are
pretty good too.

*Haley Huggins, HT (ASCP)cm*
*Technical Lab Supervisor*
*1050 Las Tablas Rd, Suite 14*
*Templeton, CA 93465*
*Office: 877-230-1518*

On Mon, Nov 6, 2017 at 10:25 AM, Cooper, Brian via Histonet <
histonet@lists.utsouthwestern.edu> wrote:

> Hi,
>
> Does anyone out there use a brand of nitrile glove that they really like?
> The ones we use here are not so good.  When we pull them on, the part
> closet to the wrist often tears off, leaving behind a nice blue rubber
> band.  At least several gloves per box have tears in them before we even
> put them on.  One of our Quality Managers asked us to come up with
> alternatives.  Can you help me out?   You can email me offline if you don't
> want to name particular brands for everyone to see.  PLEASE HELP!!!
>
> Thanks,
>
> Brian D. Cooper, HT (ASCP)CM | Histology Supervisor
> Department of Pathology and Laboratory Medicine
> Children's Hospital Los Angeles
> 4650 Sunset Blvd MS#43- Los Angeles, CA 90027
> Ph: 323.361.3357 Pager: 213-209-0184
> bcoo...@chla.usc.edu
>
>
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Re: [Histonet] Histonet Digest, Vol 167, Issue 12

[Haley Huggins via Histonet]

My suggestion for using the Dako IHC manual is not necessarily just about
the automated application, but about the antibodies themselves and what the
end results are supposed to be. It isn't the only thing you should use to
prepare for the QIHC exam, but if you want to learn about a large amount
of antibodies and what they target, the Dako IHC manual is the book of
choice. I have a friend that took the exam a few years back and that was
her suggestion as well. Yes you should know about the different platforms,
but the use of the antibodies in IHC staining will still be used to get the
targeted results provided in the Dako manual. The other platform manuals do
not provide the same detailed descriptions of the antibodies and their
purposes as the Dako manual does. In my lab, we run our IHC by hand, so I
don't use any of the automated platforms that are available today, but
luckily I know how to use most if not all of them from previous employment.
Pull from your experiences with IHC, read up from all the platforms, use
the Dako manual as a guide for a large variety of the antibodies, and check
out the study guide from Michigan Society of HIstotechnology. If you are
lucky attend a seminar on preparing for the exam. I have taken a couple of
them, they can be helpful.

Haley

*Haley Huggins, HT (ASCP)cm*
*Technical Lab Supervisor*
*1050 Las Tablas Rd, Suite 14*
*Templeton, CA 93465*
*Office: 877-230-1518*

On Tue, Oct 17, 2017 at 3:00 PM, Sally Price via Histonet <
histonet@lists.utsouthwestern.edu> wrote:

> All:
> I beg to differ with what seems to be the general consensus, but the Dako
> handbook is not really representative of the available technology within
> the IHC field and shouldn't be thought of as a prescription for
> success.  All the Dako book addresses is Dako's methods, reagents and
> instruments, which is pretty biased.  In order to properly prepare for the
> ASCPs exam, one needs to think beyond reagents and automated systems and
> REALLY understand the underlying science.  I've attended a number of
> seminars on this subject and learned that, especially because of recent
> changes to this test, one also needs to understand the clinical application
> of these procedures.  More and more we're called upon to provide
> pathologists with support in the way of procedure validation and
> troubleshooting and we need to learn a great deal more than can be learned
> from reading one vendor's publication.
> +Sally
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Re: [Histonet] QIHC Study Materials

[Haley Huggins via Histonet]

I sent to Lindsey in a private email which I thought I had hit reply all
that Michigan Society of Histotechnology also has a study guide for the
IHC, but you are right, Jay, that the Dako IHC manual is your best study
guide.

*Haley Huggins, HT (ASCP)cm*
*Technical Lab Supervisor*
*1050 Las Tablas Rd, Suite 14*
*Templeton, CA 93465*
*Office: 877-230-1518*

On Tue, Oct 17, 2017 at 2:32 AM, Jay Lundgren via Histonet <
histonet@lists.utsouthwestern.edu> wrote:

> The free one from Dako.
>
>  utm_source=link_campaign=sig-email_content=webmail>
> Virus-free.
> www.avg.com
>  utm_source=link_campaign=sig-email_content=webmail>
> <#DAB4FAD8-2DD7-40BB-A1B8-4E2AA1F9FDF2>
>
> On Mon, Oct 16, 2017 at 12:28 PM, Fairbourn, Lindsey S. via Histonet <
> histonet@lists.utsouthwestern.edu> wrote:
>
> > Hello Histonet,
> >
> > What is the best textbook to use to study for the QIHC exam?
> >
> > Thanks for your help!
> > Lindsey
> >
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[Histonet] Developing a multiplex cocktail

[Haley Huggins via Histonet]

Hello Histonetters,

It has been years since I had to create a multiplex of a dual-antibody
cocktail and for the life of me am having a brain block on the best way to
go about this. Does anyone have or know of a procedure to make this type of
cocktail?

*Haley Huggins, HT (ASCP)cm*
*Technical Lab Supervisor*
*1050 Las Tablas Rd, Suite 14*
*Templeton, CA 93465*
*Office: 877-230-1518*
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Re: [Histonet] FW: cutting a 5um frozen section from a 50um section of brain

[Haley Huggins via Histonet]

I would also be interested in knowing this tip if anyone knows it. I will
also have to check out that book. We only do cryostat sections at our lab.

*Haley Huggins, HT (ASCP)cm*
*Technical Lab Supervisor*
*1050 Las Tablas Rd, Suite 14*
*Templeton, CA 93465*
*Office: 877-230-1518*

On Fri, Oct 6, 2017 at 9:09 AM, Dessasau III, Evan via Histonet <
histonet@lists.utsouthwestern.edu> wrote:

> Hi Histonet , I have been trying to cut a FLAT 4 to 10 um frozen section
> from 50um sections of brain.  Every time I think I have the tissue flat the
> sections are never in the same plane.  I found a wonderful book in pdf
> format(A Practical Guide to Frozen Section Techniques, Stephen R. Peters)
> online with lots of wonderful tips but I'm having no luck implementing the
> tips.  Has anyone ever tried this?  Any help is GREATLY appreciated.
> Thank you,
> E-van
>
> E-van D. Dessasau, III, HTL(ASCP)cm
> Supervisor, Histology Division of Pathology
> Emory University
> Yerkes NPRC
> Main Center Rm. 2122
> 954 Gatewood Rd.
> Atlanta, GA. 30329
> (404)727-7744 lab
> (404) 727-7902 office
>
>
> 
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Re: [Histonet] Digital Pathology & Coverslipping

[Haley Huggins via Histonet]

Glass or film coverslips are fine, but you have to make sure they are
clean, no excess mounting media, or bubbles. The scanners pick up a lot of
extra things you don't want scanned. Also, check with your pathologists to
see if they have an opinion one way or another about which coverslips they
prefer.

*Haley Huggins, HT (ASCP)cm*
*Technical Lab Supervisor*
*1050 Las Tablas Rd, Suite 14*
*Templeton, CA 93465*
*Office: 877-230-1518*
*Cell: 303-652-7453*

On Fri, Aug 11, 2017 at 12:42 PM, Alexis Templeton via Histonet <
histonet@lists.utsouthwestern.edu> wrote:

> Hi All!
>
> My lab is considering moving up in the world of technology.  The goal is
> to start scanning slides for pathologists to read digitally.  We are a
> relatively high throughput lab and I'm trying to figure out what we need in
> terms of an automatic coverslipper to avoid drying time.  We currently
> still coverslip by hand and I'm assuming there would be too much wet glue
> to place the slides directly in a scanner.  Tips and recommendations,
> please!
>
> Alexis Templeton, HT (ASCP)CM
> Diagnostic Laboratory Supervisor
> Histopathology
> Texas A Veterinary Medical Diagnostic Laboratory
> P.O. Drawer 3040 | College Station, TX 77841-3040
> p: (979) 845-3414 | f: (979) 845-1794
> atemple...@tvmdl.tamu.edu
> http://tvmdl.tamu.edu
>
> We Moved!  Effective February 27, 2017 our physical (shipping) address is
> 483 Agronomy Road, College Station, TX  77840. Our billing address remains
> at PO Drawer 3040, College Station, Texas  77841
> 
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[Histonet] IHC for Lupus

[Haley Huggins via Histonet]

Is there an IHC antibody that tests for Lupus? Does anyone know? If so
which?
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[Histonet] CPT Code for PGP9.5 free floating tissue stain

[Haley Huggins via Histonet]

I am running into an issue with billing and which code is correct for
staining PGP9.5. Is it 88342? Also the billing guy asked me if thr
secondary goat, anti-mouse secondary antibody could be billed, can it? I
would appreciate any help I can get as soon as possible! He needs to bill
out today. Thanks.
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Re: [Histonet] scrubs/business casual

[Haley Huggins via Histonet]

We wear scrubs. However, we are a small reference lab and our employer
bought us 4 sets of scrubs w/embroidered company logo to wear M-Th. I added
free-scrub Friday, so we can wear whatever scrub set we want to wear on
Friday purchased on our own.

On Jun 21, 2017 6:51 AM, "O'Donnell, Bill via Histonet" <
histonet@lists.utsouthwestern.edu> wrote:

Just a little poll.

How many histology labs allow scrubs?

Of those that are allowed scrubs, do you purchase your own, or are they
provided?

Bill O'Donnell
Good Sameritan Hospital
Histology

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[Histonet] Question regarding frozen control tissue

[Haley Huggins via Histonet]

I am trying to find a way to obtain small bowel/intestine as a fresh/frozen
sample to be able to use it for a control with my staining for PGP9.5 free
floating IHC stain. Does anyone know where a good place would be to obtain
the needed control tissue?

*Haley Huggins, HT (ASCP)cm*
*Technical Lab Supervisor*
*1050 Las Tablas Rd, *
*Templeton, CA 93465*
*Office: 877-230-1518*
*Cell: 303-652-7453*
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