Re: [Histonet] Ethanol in tissue processing

Jay, Michael's post is correct and very well stated. In the US we estimate between 80 -85% of labs now use and RGA. The most common blend in the U.S is 95-5 which is 95% ethanol denatured with 5% methanol. Any company you purchase from should be happy to provide a C of A (certificate of

Re: [Histonet] FW: cutting a 5um frozen section from a 50um section of brain

I just made the aluminum temple from a 1/2 inch 6x6 piece on aluminum from Home Depot. With the sheet you will este,Italy make it look like a clock face using a sharpe. Why you want to do this is i found the aluminum plates have enough variance that if I did not always use the same side it

Re: [Histonet] Knife Sharpening Service

Try DDK (Delaware Diamond Knives), it has been some time since I used them but they were always a good choice Sent from my iPad > On Oct 5, 2016, at 5:43 PM, Jessica Riggleman via Histonet > wrote: > > Can anyone recommend a good Knife Sharpening service

Re: [Histonet] Distilled Water vs DI water for IHC and H staining

Caroline, As a vendor I have had the privilege to visit several hundred labs throughout the United States and in other countries. From what I have seen, I would say the the answer to your question is it depends on the quality of your local water source. In most cases city water systems a

Re: [Histonet] microtome validation

Ann, I would recommend validating manual microtomes more often then automatic. Any traditional gear driven microtome does change overtime as gears wear. This eventually will lead to the micron indicator on the microtome not reading what you are truly cutting. Automated, microtomes driven by