[Histonet] Precision in IHC validations

Thanks to everyone that has responded to my question. We specifically interested in the procedure for this CAP question: COM.40350 Validation of Test Performance Specifications - Modified FDA-cleared/ approved Tests and LDTs Prior to clinical use of each modified FDA-cleared or approved test

[Histonet] How are you documenting precision in IHC validations

We are working on IHC antibody validations and I want to know if there is a minimum number of slides you must run to demonstrate precision? I have not been able to find a definitive number or procedure. What is everyone doing for this? Thanks in advance for everyone's help. Martha Ward

[Histonet] Bone marrow clot IHC tissue sections washing

It was brought to my attention that we had significant washing on 3 of 8 bone marrow clot sections the other day; this is not the first time so we would like to get to the bottom of this. We use positively charged slides and all 8 cases were cut and run the same morning but allowed to air

[Histonet] antibody protocol for CXCL13

Our heme path docs are interested in this antibody and I wondered if anyone has a protocol they can share?We have the Leica Bond 3 as our staining platform/detection system. Thanks in advance for your help. Martha Ward MT(ASCP) QIHC Manager, Molecular Diagnostics Lab Atrium Health Wake

Re: [Histonet] HSV1 and HSV2

Ward-Pathology via Histonet [mailto:histonet@lists.utsouthwestern.edu] Sent: Tuesday, June 15, 2021 3:10 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] HSV1 and HSV2 CAUTION: This email is from outside HHC. USE CARE when opening attachments or links. We have always offered these

[Histonet] HSV1 and HSV2

We have always offered these two antibodies separately but recently we have begun having issues with our HSV2. In researching a new source I am seeing that some places do some sort of cocktail mixture of the two. What are others out there doing for this? Thanks in advance for your help.

[Histonet] Filed slides sticking together

I am posting this question for our Histology manager: Does anyone dry coverslipped slides in an oven before filing and if so how long and at what temperature. We are having issues with filed slides sticking together. Thanks in advance for your help with her question. Martha Ward, MT ASCP

[Histonet] Antibody validation question

When doing an initial antibody validation CAP requires at least 90% concordance between the new test and the comparator test or expected values.Are there specified requirements for sensitivity, specificity and accuracy? Thanks in advance for any help provided! Martha Ward, Manager,

[Histonet] Question about accessioning outside consult cases

We are in the process of switching to AP Beaker and in the midst of the build. My question involves how others are handling outside consult surgical and/or cytology cases. Do you assign them an unique number that identifies them as a consult as opposed to an inside case - SO20- verses

[Histonet] "Floaters" in surgical or cytology specimens

I am posting this question for a colleague in our Cytology department. How often do you see floaters on surgical or cytology specimens?Obviously we would never want to see any type of carryover but is there a standard rate published somewhere that he can reference? Thanks in advance

[Histonet] Tissue processor validation

I am posting this question for my colleague in the Histology lab. She is working on a plan for a new tissue processor validation, including special stains, and would like to get input from anyone that has already gone through it.This is the first time anyone here has ever been involved in

[Histonet] IHC validation after a new tissue processer is installed

Hello all, After a very long time our histology department is getting a new tissue processor, and hopefully several more in the near future. Of course for IHC that means a revalidation/reverification of our IHC stains. Since I have not had to do this before I wanted to get some guidance on

[Histonet] Mounting media for immunofluorescence slides

This may be an odd request but I am hoping someone out there can help us with this problem. Our institution is purchasing new digital scanners (Olympus 200) to scan all our pathology case slides. It has come to our attention that the mounting media we have all been using for cover

[Histonet] PPE for the Histology lab

Our department is looking at safety issues and one suggestion from our CLIA director is that everyone in the lab (our lab performs IHC only) should wear gloves, a gown and eye or face protection at all times and requiring it for anyone that enters the lab. We have task specific PPE

[Histonet] RUO vs IVD antibodies in immunofluorescence procedures

We are looking for IVD antibodies for C1q and C4d for use on frozen tissues (renal). What vendors are folks using for these direct immunofluorescence stains? Thanks in advance for your help. Martha Ward Wake Forest Baptist Health Winston-Salem, NC 27157

[Histonet] Looking for vendor for Zeus Wash solution

I am having trouble locating a distributor for the Zeus Wash solution (cat#103) that we use to wash our skin and renal biopsies.Cardinal and Fisher do not seem to carry it and we do not have Zeus Scientific in our system as a vendor we can use. Any help would be appreciated! Thanks,

[Histonet] Vendors closed on Presidents Day

Am I the only one that finds it odd and frustrating that some vendors are closed on Presidents Day? I just tried to call the technical service number for Dako about a problem and the message said to call back tomorrow! Hospital labs don't close and I would have thought they would at least

[Histonet] Reproducibility in IHC validations

My medical director and I are working on our procedure for reproducibility in our IHC validations and would like to know how others are handling this. How many slides do you run and over how many days/runs?If someone would share their procedure with us that would be wonderful. Thanks in

[Histonet] HPV testing on head and neck tumors and reimbursement

Hello, My billing group wanted me to ask if anyone is seeing denials for reimbursement for HPV testing on male Medicare patients when it is performed on ffpe for head and neck tumors? We have seen a few denials recently and they wanted to see how everyone is handling the coding. This is

[Histonet] Recycling formalin

I am posting this for our Histology lab manager: Is anyone recycling formalin and if so, exactly what are you using the recycled formalin for?Putting it on processors, adding to specimens, etc? Apparently our EHS department is pushing this. Also thank everyone for their help with our

[Histonet] Prostate needle biopsies

I am posting this question for our Histology manager.For prostate needle biopsies how many levels and unstained slides are people cutting and also how long after the case is signed out is everyone keeping the unstained slides that have been cut? Thanks in advance for everyone's help.

Re: [Histonet] Beaker with or without Vantage

"They" aren't the ones that will have to use it.therein lies the rub! I have not heard many positive things about AP Beaker.   Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard  \  Winston-Salem, NC 27157 p 336.716.2109  \  f 336.716.5890  

[Histonet] Looking for a C4d ffpe control block

Hello everyone, I am trying to find someone who can share a C4d ffpe control block with us. Ours is almost gone and I don't have access to any suitable tissues in house. If anyone has a block they are willing to share please contact me. I can try to help out with any controls that you may

[Histonet] Merkel cell polyomavirus antibody

Good morning, I have had a request for this antibody but I am having trouble locating a vendor for it.If anyone has any information on vendor, etc. , I would appreciate it. We have Leica Bond 3s. Thank you in advance for your help. Martha Ward Wake Forest Baptist Health

[Histonet] New IHC instrument validation process

How is everyone handling new instrument validation processes? We are looking at getting a new immunostainer.Beyond the varication process that the installers do what are our responsibilities from a lab stand point? We have a large menu of antibodies.Would it be sufficient to run one

[Histonet] Looking for a maintenance form

Does anyone have a specific form that they use to indicate that a particular instrument is functioning as expected after service repairs or PMs? We need to document this information and I would like a simple form to fill out and include with the service reports.Just trying to not have to

[Histonet] Dako Herceptest Link kit question

We just installed a new Dako Link Autostainer and have started using the Herceptest Link kit. We had been using two drop zones with our other, older instrument but that was applying only 100 uL in the 2 drop zones. With this new program we set it up to apply two zones of 200 uL but

[Histonet] PLA2R assay question

I have been asked to look into offering PLA2R on our renal biopsies and in doing some research on the subject I find that some people are staining frozen tissue via an indirect IMF procedure and some are staining ffpe tissues, also using an indirect IMF procedure.I would prefer to do this

Re: [Histonet] Blog Post Not lab related

I agreethe delete button is very easy to use. -Original Message- From: Jennifer MacDonald via Histonet [mailto:histonet@lists.utsouthwestern.edu] Sent: Thursday, April 14, 2016 12:16 PM To: Rene J Buesa Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Blog Post Not