I am curious to know if there is any interest in positive/negative control
tissue blocks or slides (FFPE) with any number of pathogens. We are an
infectious disease research center and we are always looking to expand our
contract research services. We work mostly with mouse models but from time
Hi everyone,
I am trying to stain some tissues embedded in Osteo-bed. Does anyone have a
recipe for McNeal's trichrome? Also I am having a tough time getting the
sections to stay adhered to positive charged slides. Any recommendations? The
sections are thin. Couple microns maybe.
Thanks for
Hello,
I was wondering if someone might have some expertise on how to prepare a
hard-tick for sectioning in paraffin. I have been decalcifying to soften the
exoskeleton but not sure if that even helps. I have used acid decalcifying but
would EDTA be more appropriate? I have punctured the ticks
Hi Everyone,
I am looking into doing some histology (HandE) on NHP joints. Samples will be
finger joints and wrist. If you have any experience with these larger bones
(and soft tissue) I would be interested in any advice on general time and type
of decal solution. I only need to do H no
Hi Everyone,
I am looking for a specific marker for eosinophils. Came across some testing
laboratories that offer Immunofluorescent assay for eMBP1 and EDN. Does anybody
know of a good antibody source to label these components? Is this assay
antibody based or dye based?
Thanks!
Mike
It was recently suggested to me that we might try to use our irradiator to
sterilize (not fix) tissues rather than Formalin solution. Obviously we would
need to validate this but has anyone tried this prior to IHC or IF?
Thanks!
Mike
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I'm having problems getting good cryosections of mouse lung tissue that has
been formaldehyde fixed, cryoprotected with sucrose, and embedded in OCT. The
lungs were fixed with 3% formaldehyde for 24 hours followed by 10%, 20%, and
30% sucrose until the tissue sank in solution. When sectioning
Wondering if anyone has any tips for processing human placental tissue before I
try to reinvent the wheel! (formalin fixed tissue to paraffin)
Thanks for any help!
Mike
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Anyone using ScaleA2 reagent to clear tissues for imaging? I am wondering why
the method requires Sucrose gradient followed by OCT embedding and freezing
just to turn around and thaw and clear?
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I am looking for a low cost source for activated carbon. So far I am looking at
$50 per pound for 6-14 mesh. This is for a VIP 300. Anybody use a different
mesh size?
Thanks!
Mike
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Anybody wonder who has the most harzardous job to your health? We're Number
One!!!
http://finance.yahoo.com/news/the-15-jobs-that-are-most-damaging-to-your-health-155706120.html
Mike
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Has anyone tried to embed cells grown in tissue culture? I am trying to put
some tissue culture cells through same stress as tissue would go through.
Fixation, dehydration, and heat. Any ideas? I could re-suspend in OCT and then
fix for extended time with NBF but that doesn't quite seem fair.
Thanks for all the great replies!! While I have you all worried about my cell
pellets ;-) Does anyone have a good anti-HuCD20 that works well for FFPE
tissues (cells).
Thanks!
Mike
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I recently had some tissues sent to me for frozen sectioning and staining. I
was able to section them just fine and also stained them reasonably well the
next day. The stains that I used were ones that I have used many times on
similar sections. The stains did not seem as crisp as usual but the
Does anyone have a preference over Paraplast and Paraplast plus for mouse
tissues?
Thanks!!
Mike
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In an internet search I came across a used TissueTek VIP 3000 that is in
excellent condition. This looks to be an older model and I am wondering if
anyone has any experience with this instrument? Are there any issues with parts
replacement? comes with one year warranty and they are asking
Hi Everybody!
Can you guys weigh in on the best antibodies for Human B cell and Tcell
staining in FFPE sections? We would like to have a fluorescent endpoint if that
changes things. Thanks in advance! Hope everyone has a safe Holiday.
Mike
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I am wondering if there are any service company's that will repair and service
Leica equipment in northern New York (Saranac Lake)? I have a Leica VT 1000s
that needs some attention and would need some occasional PM visits.
Thanks for any suggestions!
Mike
Does anyone have a favorite antigen retrieval method for FFPE mouse tissues
that they would be willing to share? I have been using citrate buffer Ph6.0
with poor to moderate results. Thanks for any help!
Mike
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Anybody using anti-nFAT antibodies in mouse tissues or tcells? If so, could you
recomend a particular antibody?
Thanks!
Mike
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I am looking to purchase a Processor and embedding center. I have received a
quote for VIP6 and a TEC5 but they seem to be a bit out of our price range.
Does anyone have another Processor/embedding center that they found to be
reliable?
Thanks
Mike
Hi All,
We received some frozen Baboon spleen tissue from the NIH that turned out to be
very difficult to cut. I am experienced with Mouse tissues but rarely have to
cut tissue the size of my thumb. The best information I could get was that the
tissue was not put through a sucrose gradient
I have been asked to provide cost estimates for a slide stainer and a slide
coverslipper. I have not been given time to get a quote but thought that maybe
someone would have a reasonable idea of the cost. a range of cost would be fine
also.
Thanks for any help!
Mike
I may have the chance to get a tissue processor (VIP 2000 or 3000). Are these
units still serviceable? Is it hard to find parts? Are they Reliable?
Thanks for any help!!
Mike
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I would like cut fresh spleen and lymph node sections on a vibrating microtome.
I have tried embedding in low melt agarose but the tissue seems to be too
flexable to get good repeating sections (uniform thickness). When the knife
does catch the cells seem to pour out into the surrounding
I have recently tried to order a product from ihcworld and had no luck at all.
Has anyone had this problem or is there anyone out there that is familiar with
this company/website. I think that selling products may be new to this website.
Any suggestions?
Thanks!
Mike
I am having trouble cutting FFPE liver sections. I have embedded the tissue
manually going from fomalin to water to increasing ethanol to xylene and to
paraffin. I can get good sections by soaking the block with ice cold water
before taking a few sections but then have to re-soak in order to
I am looking for a conference/course to attend in 2010. I am experienced in IF
and IHC and would like to learn more about any new techniques and products that
might be available. Does anyone have a recommendation for a course or a
conference in the US? I am not a certified HT but I pretend to
Does anyone know of a company(s) that sells/makes ISH probes to viral RNA (PR8
and or Sendai) or a company that will make custom probes.
Thanks for any help!!
Mike
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I am attempting to do IF on FFPE sections but the history/treatment of my
tissues (mouse tissues) is unknown. I would like to recommend to our labs a
strategy for tissue collection, length of time in NBF, type of antigen
retrieval, and so on. Would anyone (or everyone) be willing to share
Has anyone compared Cytoseal 60 with Cytoseal XYL? I clear with Xylene and use
cytoseal 60 (Toluene)to coverslip. Seems like it would make sense to use
Cytoseal XYL (Xylene) but they only sell in a case and I can't get a sample. I
would be interested to know if anyone has a preference.
We have taken sections from OCT embedded tissues and processed them for RNA
analysis with out any problems. You should be careful to move the tissue to a
solution that will protect the RNA once it has been either sectioned or thawed.
Good Luck!
Trudeau Institute email service will be
We have a BSL3 laboratory with a cryostat (TB research). The cryostat user
asked me for the best method to decontaminate the cryostat. My suggestion was
to clean the cryostat (while cold) with 95% ethanol followed by absolute
ethanol after wards. Then to defrost the cryostat and clean with a
I am am having a difficult time getting immuno fluorescent staining in mouse
liver. I am using markers that work side by side in lung tissue (F4/80, GR1).
Does anyone have any suggestions for Immuno Fluorescent staining in mouse
tissue or similar problems?
Thanks!!
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