[Histonet] Precision in IHC validations

[Martha Ward-Pathology via Histonet]

Thanks to everyone that has responded to my question.

We specifically interested in the procedure for this CAP question:

COM.40350 Validation of Test Performance Specifications - Modified FDA-cleared/
approved Tests and LDTs

Prior to clinical use of each modified FDA-cleared or approved test and 
laboratory developed
tests (LDTs), the laboratory has performed a validation study and prepared a
written assessment of each of the following test method performance 
specifications, as
applicable, using a sufficient number of characterized samples:

● Analytical accuracy
● Analytical precision
● Reportable range
● Analytical sensitivity (lower detection limit)
● Analytical specificity
● Any other performance characteristic required to ensure analytical test 
performance




Do you comment at all regarding precision…..do you run the same slide each day 
over 3 days, or something like that?
NOTE 3: Precision is validated by repeat measurement of samples at varying 
concentrations or
activities within-run and between-run over a period of time.

We are just not sure what number of repeats is best…..




This electronic message is intended only for the use of the individual(s) and 
entity named as recipients in the message. If you are not an intended recipient 
of this message, please notify the sender immediately and delete the material 
from any computer. Do not deliver, distribute or copy this message, and do not 
disclose its contents or take any action in reliance on the information it 
contains. Thank you.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] How are you documenting precision in IHC validations

[Martha Ward-Pathology via Histonet]

We are working on IHC antibody validations and I want to know if there is a 
minimum number of slides you must run to demonstrate precision? I have not 
been able to find a definitive number or procedure.   What is everyone doing 
for this?

Thanks in advance for everyone's help.

Martha Ward
Atrium Health Wake Forest Baptist



This electronic message is intended only for the use of the individual(s) and 
entity named as recipients in the message. If you are not an intended recipient 
of this message, please notify the sender immediately and delete the material 
from any computer. Do not deliver, distribute or copy this message, and do not 
disclose its contents or take any action in reliance on the information it 
contains. Thank you.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Bone marrow clot IHC tissue sections washing

[Martha Ward-Pathology via Histonet]

It was brought to my attention that we had significant washing on  3 of 8 bone 
marrow clot sections the other day; this is not the first time so we would like 
to get to the bottom of this.   We use positively charged slides and all 8 
cases were cut and run the same morning but allowed to air dry and then bake at 
60C for 20 minutes before being run on our Bond 3 stainer.   Has anyone out 
there experienced this type of problem and if so, what were your solutions?
The repeat of the 3 cases today showed similar washing of tissue.

This hasn't just started but has occurred periodically but the pathologists 
have tried to live with it and usually we can finally get enough tissue to stay 
on after 1-2 attempts.   Suggestions include cutting and drying the slides 
overnight and/or going to a gelatinated  slide versus a sialylated slide.   We 
have been using this particular brand of positive charged slide with good 
results for several years and rarely have issues with other tissue types unless 
they are particularly bloody.

Thoughts or suggestions are greatly appreciated.


Martha Ward MT(ASCP) QIHC
Atrium Health Wake Forest Baptist



___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] antibody protocol for CXCL13

[Martha Ward-Pathology via Histonet]

Our heme path docs are interested in this antibody and I wondered if anyone has 
a protocol they can share?We have the Leica Bond 3 as our staining 
platform/detection system.

Thanks in advance for your help.


Martha Ward MT(ASCP) QIHC
Manager, Molecular Diagnostics Lab
Atrium Health Wake Forest Baptist Health




___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] HSV1 and HSV2

[Martha Ward-Pathology via Histonet]

Thank you for the information.   I will look into your antibody source.

Martha

-Original Message-
From: Cartun, Richard  
Sent: Tuesday, June 15, 2021 3:26 PM
To: Martha Ward-Pathology 
Cc: histonet@lists.utsouthwestern.edu
Subject: [EXTERNAL] RE: HSV1 and HSV2

Hi Martha:

We are using type-specific monoclonals from BioSB (Santa Barbara, CA).  They 
also sell a cocktail of the two antibodies which is what we use for screening.  
Rarely, do we get requests for type-specific identification.

Richard

Richard W. Cartun, MS, PhD
Director, Histology & The Martin M. Berman, MD Immunopathology/Morphologic 
Proteomics Laboratory Director, Biospecimen Collection Programs Assistant 
Director, Anatomic Pathology Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 972-1596 (Office)
(860) 545-2204 (Fax)
richard.car...@hhchealth.org


-Original Message-
From: Martha Ward-Pathology via Histonet 
[mailto:histonet@lists.utsouthwestern.edu]
Sent: Tuesday, June 15, 2021 3:10 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] HSV1 and HSV2

CAUTION: This email is from outside HHC. USE CARE when opening attachments or 
links.

We have always offered these two antibodies separately but recently we have 
begun having issues with our HSV2.   In researching a new source I am seeing 
that some places do some sort of cocktail mixture of the two.   What are others 
out there doing for this?

Thanks in advance for your help.

Martha Ward, Manager
Molecular Diagnostics  Lab
Wake Forest Baptist Medical Center
Winston-Salem, NC 27157
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!KCs9X-8!PM-BBy3bF7epr83ucPrDa0D21BtxagGkNt2mKWWY7SUaExNwbpL43czZRyw5F0A-LMQ$


Reminder: This e-mail and any attachments are subject to the current HHC email 
retention policies. Please save or store appropriately in accordance with 
policy.

This e-mail message, including any attachments, is for the sole use of the 
intended recipient(s) and may contain confidential and privileged information. 
Any unauthorized review, use, disclosure, or distribution is prohibited. If you 
are not the intended recipient, or an employee or agent responsible for 
delivering the message to the intended recipient, please contact the sender by 
reply e-mail and destroy all copies of the original message, including any 
attachments.

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] HSV1 and HSV2

[Martha Ward-Pathology via Histonet]

We have always offered these two antibodies separately but recently we have 
begun having issues with our HSV2.   In researching a new source I am seeing 
that some places do some sort of cocktail mixture of the two.   What are others 
out there doing for this?

Thanks in advance for your help.

Martha Ward, Manager
Molecular Diagnostics  Lab
Wake Forest Baptist Medical Center
Winston-Salem, NC 27157
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Filed slides sticking together

[Martha Ward-Pathology via Histonet]

I am posting this question for our Histology manager:

Does anyone dry coverslipped slides in an oven before filing and if so how long 
and at what temperature.  We are having issues with filed slides sticking 
together.


Thanks in advance for your help with her question.

Martha  Ward, MT ASCP (QIHC)
Manager, Molecular Diagnostics Lab
Wake Forest Baptist Medical Center
Winston-Salem, NC 27157

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Antibody validation question

[Martha Ward-Pathology via Histonet]

When doing an initial antibody validation CAP requires at least 90% concordance 
between the new test and the comparator test or expected values.Are there 
specified requirements for sensitivity,  specificity and accuracy?

Thanks in advance for any help provided!

Martha Ward, Manager, Molecular Diagnostics Lab
Wake Forest Baptist Medical Center
Winston-Salem, NC 27157
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Question about accessioning outside consult cases

[Martha Ward-Pathology via Histonet]

We are in the process of switching to AP Beaker and in the midst of the build.  
  My question involves how others are handling outside consult surgical and/or 
cytology cases.   Do you assign them an unique number that identifies them as a 
consult as opposed to an inside case - SO20- verses S20-? We 
currently assign them as just another surgical case but I know some places do 
have outside consult designations.

What is everyone doing?

Thanks in advance for your input.

Martha Ward, MT ASCP (QIHC)
Manager, Molecular Diagnostics Lab
Wake Forest Baptist Medical Center
Winston-Salem, NC 27157
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] "Floaters" in surgical or cytology specimens

[Martha Ward-Pathology via Histonet]

I am posting this question for a colleague in our Cytology department. How 
often do you see floaters on surgical or cytology specimens?Obviously we 
would never want to see any type of carryover but is there a standard rate 
published somewhere that he can reference?

Thanks in advance for your help.

Martha Ward, MT ASCP, QIHC
Manager, Molecular Diagnostics Lab
Wake Forest Baptist Medical Center
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Tissue processor validation

[Martha Ward-Pathology via Histonet]

I am posting this question for my colleague in the Histology lab.   She is 
working on a plan for a new tissue processor validation, including special 
stains, and would like to get input from anyone that has already gone through 
it.This is the first time anyone here has ever been involved in validating 
a new processor.   Current ones are 25+ years.


Any help or advice would be appreciated.   Thanks in advance!

Martha Ward, MT (ASCP) QIHC
Wake Forest Baptist Health
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] IHC validation after a new tissue processer is installed

[Martha Ward-Pathology via Histonet]

Hello all,

After a very long time our histology department is getting a new tissue 
processor, and hopefully several more in the near future.   Of course for IHC 
that means a revalidation/reverification of our IHC stains.  Since I have not 
had to do this before I wanted to get some guidance on how to go about doing 
this.  I feel like I have to completely revalidate the ER, PR and Her2 (20 
positive, 20 negative) but wasn't sure about the other antibodies.   We have a 
menu of around 130do I have to test every one of them or can we chose a 
representative sample? How many antibodies would you suggest?   How many 
positive and negative cases of each should we run?   It says it is left up to 
the medical director but with our CAP inspection next spring we want to make 
sure we will be fully prepared.What have others done?

Thank you in advance for your help with this.


Martha Ward, MT (ASCP) QIHC
Wake Forest Baptist Medical Center
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Mounting media for immunofluorescence slides

[Martha Ward-Pathology via Histonet]

This may be an odd request but I am hoping someone out there can help us with 
this problem. Our institution is purchasing new digital scanners (Olympus 
200) to scan all our pathology case slides. It has come to our attention 
that the mounting media we have all been using for cover slipping our direct 
immunofluorescence slides (skins and renal biopsies using FITC IgG, etc. 
antibodies) is incompatible with the use of these new scanners.   We use a hard 
set mounting media from Vector but it is never quite hard enough to prevent any 
movement of the cover slip, which is apparently critical to the scanning 
process and potentially damaging to the scanner.The techs that are using 
the scanners are advising us to use a permanent mounting media such as Cytoseal 
60. Has anyone encountered this problem and was there any downside to using 
something like Cytoseal 60 on the slides?   We plan to test a few control 
slides to see how they look.

Thanks in advance for any advice you can give me.

Martha Ward
Molecular Diagnostics Lab
Wake Forest Baptist Health
Winston-Salem, NC 27157
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] PPE for the Histology lab

[Martha Ward-Pathology via Histonet]

Our department is looking at safety issues and one suggestion from our CLIA 
director is that everyone in the lab (our lab performs IHC only) should wear 
gloves, a gown and eye or face protection at all times and requiring it for 
anyone that enters the lab.   We have task specific PPE requirements for these 
items to be worn when changing solutions on the stainers or working with serum 
for our indirect immunofluorescence for example but don't require it for FFPE 
microtomy or loading the IHC stainers.   What are others requiring in their 
labs?

Thanks in advance for your feedback on this issue.

Martha Ward
Manager, Molecular Diagnostics Lab
Wake Forest Baptist Health
Winston-Salem, NC 27157
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] RUO vs IVD antibodies in immunofluorescence procedures

[Martha Ward-Pathology via Histonet]

We are looking for IVD antibodies for C1q and C4d for use on frozen tissues 
(renal). What vendors are folks using for these direct immunofluorescence 
stains?

Thanks in advance for your help.

Martha Ward
Wake Forest Baptist Health
Winston-Salem, NC 27157
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Looking for vendor for Zeus Wash solution

[Martha Ward-Pathology via Histonet]

I am having trouble locating a distributor for the Zeus Wash solution (cat#103) 
that we use to wash our skin and renal biopsies.Cardinal and Fisher do not 
seem to carry it and we do not have Zeus Scientific in our system as a vendor 
we can use.   Any help would be appreciated!

Thanks,

Martha Ward
Wake Forest Baptist Health
Winston-Salem, NC 27157
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Vendors closed on Presidents Day

[Martha Ward-Pathology via Histonet]

Am I the only one that finds it odd and frustrating that some vendors are 
closed on Presidents Day?   I just tried to call the technical service number 
for Dako about a problem and the message said to call back tomorrow!

Hospital labs don't close and I would have thought they would at least have 
someone staffing the number to cover issues that come up.   Thankfully my call 
isn't urgent but it could have been and then I would be stuck.


Martha Ward
Wake Forest Baptist Medical Center
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Reproducibility in IHC validations

[Martha Ward-Pathology via Histonet]

My medical director and I are working on our procedure for reproducibility in 
our IHC validations and would like to know how others are handling this. 
How many slides do you run and over how many days/runs?If someone would 
share their procedure with us that would be wonderful.
Thanks in advance for your help.
Martha Ward
Wake Forest Baptist Health
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] HPV testing on head and neck tumors and reimbursement

[Martha Ward-Pathology via Histonet]

Hello,

My billing group wanted me to ask if anyone is seeing denials for reimbursement 
for HPV testing on male Medicare patients when it is performed on ffpe for head 
and neck tumors? We have seen a few denials recently and they wanted to see 
how everyone is handling the coding.   This is for CPT code 87624.

Thank you in advance for any input you may have on this issue.


Martha Ward, MT (ASCP) QIHC
Wake Forest Baptist Medical Center
Winston-Salem, NC 27157

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Recycling formalin

[Martha Ward-Pathology via Histonet]

I am posting this for our Histology lab manager:

Is anyone recycling formalin and if so, exactly what are you using the recycled 
formalin for?Putting it on processors, adding to specimens, etc?
Apparently our EHS department is pushing this.


Also thank everyone for their help with our questions about unstained slides 
for prostate biopsies.It was very helpful.

Thanks in advance for your help with our recycled formalin question.

Martha Ward
Wake Forest Baptist Health
336-716-2109
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Prostate needle biopsies

[Martha Ward-Pathology via Histonet]

I am posting this question for our Histology manager.For prostate needle 
biopsies how many levels and unstained slides are people cutting and also how 
long after the case is signed out is everyone keeping the unstained slides that 
have been cut?

Thanks in advance for everyone's help.

Martha Ward
Wake Forest Baptist Health
Winston-Salem, NC 25157
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] Beaker with or without Vantage

[Martha Ward-Pathology via Histonet]

"They" aren't the ones that will have to use it.therein lies the rub!   I 
have not heard many positive things about AP Beaker.


 
Martha Ward, MT (ASCP) QIHC
Manager

Molecular Diagnostics Lab
Medical Center Boulevard  \  Winston-Salem, NC 27157
p 336.716.2109  \  f 336.716.5890  
mw...@wakehealth.edu  
 
 



-Original Message-
From: Morken, Timothy via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Tuesday, November 28, 2017 11:48 AM
To: Histonet
Subject: Re: [Histonet] Beaker with or without Vantage

I am interested as well. "They" are threatening us with a move to Beaker in the 
future


Tim Morken
Pathology Site Manager, Parnassus
Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of 
Pathology UC San Francisco Medical Center

-Original Message-
From: Blake Taylor via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Tuesday, November 28, 2017 8:10 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Beaker with or without Vantage

I'm looking for anyone out there that has switched to Beaker for AP, Also do 
you use Ventana Connect with Beaker?  Did you choose to use the Beaker tracking 
system or is anyone using Beaker in conjunction with Vantage?  Our Hospital is 
in the beginning phase of moving from Copath Sunquest to Beaker (2017 version) 
.  Any thoughts of what has gone well and what has not would be appreciated.

Thanks so much

Blake Taylor
Surgical Pathology Supervisor
Lexington Medical Center
803-936-8214
bcdu...@lexhealth.org

PRIVILEGED AND CONFIDENTIAL: This electronic message and any attachments are 
confidential property of the sender. The information is intended only for the 
use to the person to whom it was addressed. Any other interception, copying, 
accessing, or disclosure of this message is prohibited. The sender takes no 
responsibility for any unauthorized reliance on this message. If you have 
received this message in error, please immediately notify the sender and purge 
the message you received. Do not forward this message without permission.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Looking for a C4d ffpe control block

[Martha Ward-Pathology via Histonet]

Hello everyone,

I am trying to find someone who can share a C4d ffpe control block with us.  
Ours is almost gone and I don't have access to any suitable tissues in house.   
If anyone has a block they are willing to share please contact me.   I can try 
to help out with any controls that you may be in need of as well.

Thanks in advance for everyone's help.

Martha Ward, MT (ASCP) QIHC
Manager, Molecular Diagnostics Lab
Wake Forest Baptist Health
Winston-Salem, NC 27157
mw...@wakehealth.edu

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Merkel cell polyomavirus antibody

[Martha Ward-Pathology via Histonet]

Good morning,

I have had a request for this antibody but I am having trouble locating a 
vendor for it.If anyone has any information on vendor, etc. , I would 
appreciate it.   We have Leica Bond 3s.

Thank you in advance for your help.

Martha Ward
Wake Forest Baptist Health

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] New IHC instrument validation process

[Martha Ward-Pathology via Histonet]

How is everyone handling new instrument validation processes?   We are looking 
at getting a new immunostainer.Beyond the varication process that the 
installers do what are our responsibilities from a lab stand point?   We have a 
large menu of antibodies.Would it be sufficient to run one of each antibody 
to demonstrate that the new instrument compares to the old.The detection 
kits, retrieval solutions, antibodies, etc. would all remain the same.

Thanks in advance for your help.

Martha Ward
Molecular Diagnostics Lab
Wake Forest Baptist Health
Winston-Salem, NC 27157
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Looking for a maintenance form

[Martha Ward-Pathology via Histonet]

Does anyone have a specific form that they use to indicate that a particular 
instrument is functioning as expected after service repairs or PMs?   We need 
to document this information and I would like a simple form to fill out and 
include with the service reports.Just trying to not have to reinvent the 
wheel and would like to see what others are doing.

Thanks in advance for your help.

Martha Ward
Molecular Diagnostics Lab
Wake Forest Baptist Health
Winston-Salem, NC 27157
336-716-2109
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] FT OVERNIGHT HT POSTITION Delray Beach, FL

[Delray Beach Pathology Kari Simeone via Histonet]

Hi Histonetters! We are looking for a full time licensed HISTOTECHNOLOGIST here 
in our very busy Delray Beach Florida Dermatology Lab. This is a permanent full 
time NIGHT SHIFT (40 hours) position with benefits (medical/401k/vacation) and 
shift differential. THIS IS A DRUG FREE WORKPLACE. Background check, 
personality test and drug test will be necessary. Sorry, no relocation 
assistance provided.


***PLEASE NO HEAD HUNTERS/PLACEMENT SERVICES***!!!

Please fill out employment application HERE
http://www.indeed.com/m/viewjob?jk=266fccd1fa688bb6=serp
^^you MUST follow this application link to apply! No exceptions.

*FULL TIME position Mon-Fri OR Sun-Thurs 10p-6:30AM
*MUST be licensed as a FL HISTOTECHNOLOGIST ONLY (will be working solo most of 
your shift)
*MUST have at LEAST FIVE (5) years experience (dermatology preferred)
 Please DO NOT respond if no EXPERIENCE!
*VERY proficient in embedding and microtomy
*WILL MOSTLY BE EMBEDDING EXCISION BLOCKS, please know DERMS
*must be self motivated, reliable and a team player
*knowledge in operating Ventana and Leica equipment desired (not necessary)
*some IHC experience preferred but not necessary



Kari M Simeone

Histology/Immunohistochemistry Specialist Supervisor

Alternate Laboratory Supervisor

Delray Beach Technical Laboratory

ADCS Clinics, LLC

Advanced Dermatology and Cosmetic Surgery

Ameriderm

www.advancedderm.com





The information contained in this message and any attachments is intended only
for the use of the individual or entity to which it is addressed, and may 
contain
information that is privileged, confidential and exempt from disclosure under
applicable law. If you have received this message in error, you are prohibited
from copying, distributing or using the information. Please contact the sender
immediately by return e-mail and delete the original message.

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Dako Herceptest Link kit question

[Martha Ward-Pathology via Histonet]

We just installed a new Dako Link Autostainer and have started using the 
Herceptest Link kit.   We had been using two drop zones with our other, older 
instrument but that was applying only 100 uL in the 2 drop zones.   With this 
new program we  set it up to apply two zones of 200 uL but discovered that we 
will be cutting our test capability in half  (50 to about 25 slides).  How are 
other users handling this?   Do you use two drop zones, one on each end to 
cover the control and case material or do you have the reagents apply in one 
drop zone in the middle of the slide?  I am just concerned about the reagents 
spreading evenly across the slide.   What is everyone else's experience with 
this issue?

Thanks in advance for your help!

Martha Ward
Wake Forest Baptist Health

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] FT OVERNIGHT HT POSTITION Delray Beach, FL

[Delray Beach Pathology Kari Simeone via Histonet]

Hi Histonetters! We are looking for a full time licensed HISTOTECHNOLOGIST here 
in our very busy Delray Florida Dermatology Lab. This is a permanent full time 
NIGHT SHIFT (40 hours) position with benefits (medical/401k/vacation) and shift 
differential. THIS IS A DRUG FREE WORKPLACE. Background check, personality test 
and drug test will be necessary. Sorry, no relocation assistance provided.


***PLEASE NO HEAD HUNTERS/PLACEMENT SERVICES***!!!

Please fill out employment application HERE
http://www.indeed.com/m/viewjob?jk=266fccd1fa688bb6=serp
^^you MUST follow this application link to apply! No exceptions.

*full time position Mon-Fri OR Sun-Thurs 10p-6:30AM
*MUST be licensed as a FL HISTOTEHCNOLOGIST ONLY (will be working solo most of 
your shift)
*MUST have at LEAST FIVE (5) years experience (dermatology preferred)
 Please DO NOT respond if no EXPERIENCE!
*VERY proficient in embedding and microtomy
*WILL MOSTLY BE EMBEDDING EXCISION BLOCKS, please know DERMS
*must be self motivated, reliable and a team player
*knowledge in operating Ventana and Leica equipment desired (not necessary)
*some IHC experience preferred but not necessary



Kari M Simeone

Histology/Immunohistochemistry Specialist Supervisor

Alternate Laboratory Supervisor

Delray Beach Technical Laboratory

ADCS Clinics, LLC

Advanced Dermatology and Cosmetic Surgery

Ameriderm

www.advancedderm.com







The information contained in this message and any attachments is intended only
for the use of the individual or entity to which it is addressed, and may 
contain
information that is privileged, confidential and exempt from disclosure under
applicable law. If you have received this message in error, you are prohibited
from copying, distributing or using the information. Please contact the sender
immediately by return e-mail and delete the original message.

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] FT OVERNIGHT HT POSTITION Delray Beach, FL

[Delray Beach Pathology Kari Simeone via Histonet]

Hi Histonetters! We are looking for a full time licensed HISTOTECHNOLOGIST here 
in our very busy Delray Florida Dermatology Lab. This is a permanent full time 
NIGHT SHIFT (40 hours) position with benefits (medical/401k/vacation) and shift 
differential. THIS IS A DRUG FREE WORKPLACE. Background check, personality test 
and drug test will be necessary. Sorry, no relocation assistance provided.


***PLEASE NO HEAD HUNTERS/PLACEMENT SERVICES***!!!

Please fill out employment application HERE
http://www.indeed.com/m/viewjob?jk=266fccd1fa688bb6=serp
^^you MUST follow this application link to apply! No exceptions.

*full time position Mon-Fri OR Sun-Thurs 10p-6:30AM
*MUST be licensed as a FL HISTOTEHCNOLOGIST ONLY (will be working solo most of 
your shift)
*MUST have at LEAST FIVE (5) years experience (dermatology preferred)
 Please DO NOT respond if no EXPERIENCE!
*VERY proficient in embedding and microtomy
*WILL MOSTLY BE EMBEDDING EXCISION BLOCKS, please know DERMS
*must be self motivated, reliable and a team player
*knowledge in operating Ventana and Leica equipment desired (not necessary)
*some IHC experience preferred but not necessary



Kari M Simeone

Histology/Immunohistochemistry Specialist Supervisor

Alternate Laboratory Supervisor

Delray Beach Technical Laboratory

ADCS Clinics, LLC

Advanced Dermatology and Cosmetic Surgery

Ameriderm

www.advancedderm.com







The information contained in this message and any attachments is intended only
for the use of the individual or entity to which it is addressed, and may 
contain
information that is privileged, confidential and exempt from disclosure under
applicable law. If you have received this message in error, you are prohibited
from copying, distributing or using the information. Please contact the sender
immediately by return e-mail and delete the original message.

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] FT OVERNIGHT HT POSTITION Delray Beach, FL

[Delray Beach Pathology Kari Simeone via Histonet]

Hi Histonetters! We are looking for a full time licensed HISTOTECHNOLOGIST here 
in our very busy Delray Florida Dermatology Lab. This is a permanent full time 
NIGHT SHIFT (40 hours) position with benefits (medical/401k/vacation) and shift 
differential. THIS IS A DRUG FREE WORKPLACE. Background check, personality test 
and drug test will be necessary. Sorry, no relocation assistance provided.


***PLEASE NO HEAD HUNTERS/PLACEMENT SERVICES***!!!

Please fill out employment application HERE
http://www.indeed.com/m/viewjob?jk=266fccd1fa688bb6=serp
^^you MUST follow this application link to apply! No exceptions.

*full time position Mon-Fri OR Sun-Thurs 10p-6:30AM
*MUST be licensed as a FL HISTOTEHCNOLOGIST ONLY (will be working solo most of 
your shift)
*MUST have at LEAST FIVE (5) years experience (dermatology preferred)
 Please DO NOT respond if no EXPERIENCE!
*VERY proficient in embedding and microtomy
*WILL MOSTLY BE EMBEDDING EXCISION BLOCKS, please know DERMS
*must be self motivated, reliable and a team player
*knowledge in operating Ventana and Leica equipment desired (not necessary)
*some IHC experience preferred but not necessary



Kari M Simeone

Histology/Immunohistochemistry Specialist Supervisor

Alternate Laboratory Supervisor

Delray Beach Technical Laboratory

ADCS Clinics, LLC

Advanced Dermatology and Cosmetic Surgery

Ameriderm

www.advancedderm.com





The information contained in this message and any attachments is intended only
for the use of the individual or entity to which it is addressed, and may 
contain
information that is privileged, confidential and exempt from disclosure under
applicable law. If you have received this message in error, you are prohibited
from copying, distributing or using the information. Please contact the sender
immediately by return e-mail and delete the original message.

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] FT OVERNIGHT HT POSTITION Delray Beach, FL

[Delray Beach Pathology Kari Simeone via Histonet]

Hi Histonetters! We are looking for a full time licensed HISTOTECHNOLOGIST here 
in our very busy Delray Florida Dermatology Lab. This is a permanent full time 
NIGHT SHIFT (40 hours) position with benefits (medical/401k/vacation) and shift 
differential. THIS IS A DRUG FREE WORKPLACE. Background check, personality test 
and drug test will be necessary. Sorry, no relocation assistance provided.


***PLEASE NO HEAD HUNTERS/PLACEMENT SERVICES***!!!

Please fill out employment application HERE
http://www.indeed.com/m/viewjob?jk=266fccd1fa688bb6=serp
^^you MUST follow this application link to apply! No exceptions.

*full time position Mon-Fri OR Sun-Thurs 10p-6:30AM
*MUST be licensed as a FL HISTOTEHCNOLOGIST ONLY (will be working solo most of 
your shift)
*MUST have at LEAST FIVE (5) years experience (dermatology preferred)
 Please DO NOT respond if no EXPERIENCE!
*VERY proficient in embedding and microtomy
*WILL MOSTLY BE EMBEDDING EXCISION BLOCKS, please know DERMS
*must be self motivated, reliable and a team player
*knowledge in operating Ventana and Leica equipment desired (not necessary)
*some IHC experience preferred but not necessary



Kari M Simeone

Histology/Immunohistochemistry Specialist Supervisor

Alternate Laboratory Supervisor

Delray Beach Technical Laboratory

ADCS Clinics, LLC

Advanced Dermatology and Cosmetic Surgery

Ameriderm

www.advancedderm.com





The information contained in this message and any attachments is intended only
for the use of the individual or entity to which it is addressed, and may 
contain
information that is privileged, confidential and exempt from disclosure under
applicable law. If you have received this message in error, you are prohibited
from copying, distributing or using the information. Please contact the sender
immediately by return e-mail and delete the original message.

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] HHV-8 RTU

[Delray Beach Pathology Kari Simeone via Histonet]

My vendor (Cell Marque/Sigma Aldrich) has a YEAR backorder on this IHC RTU. 
Does anyone have a vendor they purchase from that I can research? Thanks so 
much.



Kari M Simeone

Histology/Immunohistochemistry Specialist Supervisor

Alternate Laboratory Supervisor









The information contained in this message and any attachments is intended only
for the use of the individual or entity to which it is addressed, and may 
contain
information that is privileged, confidential and exempt from disclosure under
applicable law. If you have received this message in error, you are prohibited
from copying, distributing or using the information. Please contact the sender
immediately by return e-mail and delete the original message.

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] PLA2R assay question

[Martha Ward-Pathology via Histonet]

I have been asked to look into offering PLA2R on our renal biopsies and in 
doing some research on the subject I find that some people are staining frozen 
tissue via an indirect IMF procedure and some are staining ffpe tissues, also 
using an indirect IMF procedure.I would prefer to do this via traditional 
IHC (we have Bond 2 stainers).Is anyone doing this way?   If so, could you 
provide some tips on getting it to work successfully?

Thanks in advance for any help.


 
Martha Ward, MT (ASCP) QIHC
Manager
 
Molecular Diagnostics Lab
Medical Center Boulevard  \  Winston-Salem, NC 27157
p 336.716.2109  \  f 336.716.5890  
mw...@wakehealth.edu  


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] Blog Post Not lab related

[Martha Ward-Pathology via Histonet]

I agreethe delete button is very easy to use.  



-Original Message-
From: Jennifer MacDonald via Histonet 
[mailto:histonet@lists.utsouthwestern.edu] 
Sent: Thursday, April 14, 2016 12:16 PM
To: Rene J Buesa
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Blog Post Not lab related

In addition to the Histonet, I am on a listserve for clinical laboratory 
educators.  Dr. Raff posts his blog link on that listserve as well.   He 
also responds to some of the questions.  There has never been one public 
complaint regarding his posts.  It is very clear in the subject line and the 
ability to delete his posts are quite easy.  Please don't give credence to the 
myth that the clinical lab has more patience and common sense than the 
histology world. 



From:   Rene J Buesa via Histonet 
To: Caroline Miller , Lester Raff MD 

Cc: "histonet@lists.utsouthwestern.edu" 

Date:   04/14/2016 06:02 AM
Subject:Re: [Histonet] Blog Post Not lab related



Amen!!!But you have to concede that Lester is a very  persistent, almost 
obstinate individual, probably used to impose his will and this postings are 
just an example of it: he likes his blog and tries to impose it to everyone. 
Evidently he has all the time in the world and just does not know what to do 
with it and enjoys sharing his "witty" side. René 

On Wednesday, April 13, 2016 3:24 PM, Caroline Miller via Histonet 
 wrote:
 

 Lester, I do believe this is the second 'non lab related' blog post this
week. As we have spoken about before I do not think histonet is an
appropriate place for your blog posts. I, personally, was totally OK with
you co-posting with other lab related topics. However you are now pushing
it and I respectfully ask that you refrain from sending out your blog 
posts
to the whole list, let people sign up if they are interested.

The issue is that this is a histology related list, if everyone posted
non-lab stuff here it would soon be chaos!

Respectfully yours,
mills

On Wed, Apr 13, 2016 at 11:38 AM, Lester Raff MD via Histonet <
histonet@lists.utsouthwestern.edu> wrote:

> Just some philosophy towards the end of a long day.
>
>
> 
http://www.chicagonow.com/downsize-maybe/2016/04/kitten-power-can-get-things-done/

>
>
>
> Just a reminder-I try to limit my blog invitations here. If you enjoy 
the
> blog, remember to subscribe (no charge, no spam) on the ChicagoNow blog
> site.
>
> Thanks for your readership.
>
> Lester J. Raff, MD MBA
> UroPartners
> Medical Director Of Laboratory
> 2225 Enterprise Dr. Suite 2511
> Westchester, Il 60154
> Tel: 708-486-0076
> Fax: 708-492-0203
>
> ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>



-- 
Caroline Miller (mills)
Director of Histology
3Scan.com
415 2187297
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


 
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] TCR alpha/beta gamma/delta

[Delray Beach Pathology Kari Simeone via Histonet]

Is anyone out in Histoland performing TCR for alpha/beta & gamma/delta via IHC 
on the Leica Bond? If so, mind sharing your protocols and vendor information 
with me? Pretty please? Looking to bring this on. I appreciate it in advance.



Kari M Simeone

Histology/Immunohistochemistry Specialist Supervisor

Alternate Laboratory Supervisor

Delray Beach Technical Laboratory

ADCS Clinics, LLC

ksime...@leavittmgt.com







The information contained in this message and any attachments is intended only
for the use of the individual or entity to which it is addressed, and may 
contain
information that is privileged, confidential and exempt from disclosure under
applicable law. If you have received this message in error, you are prohibited
from copying, distributing or using the information. Please contact the sender
immediately by return e-mail and delete the original message.

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] SOX 10 Cellmarque (Edison Narvaez)

[Delray Beach Pathology Kari Simeone via Histonet]

Hi Edison. I use the CM SOX-10 polyclonal RTU on my Leica Bond-Max(s). I also 
run it with Leica's red kit. I have had zero issues. I do use CM's SOX-10 
controls though, they seem to stain the best for me. My protocol is ER2 for 30 
minutes at 97 degrees. I run them down from 100%-SUB-X and have no running or 
bleeding. Good luck, this protocol has been issue free for me for years.



Kari M Simeone

Histology/Immunohistochemistry Specialist Supervisor

Alternate Laboratory Supervisor

Delray Beach Technical Laboratory

ADCS Clinics, LLC

Advanced Dermatology and Cosmetic Surgery







The information contained in this message and any attachments is intended only
for the use of the individual or entity to which it is addressed, and may 
contain
information that is privileged, confidential and exempt from disclosure under
applicable law. If you have received this message in error, you are prohibited
from copying, distributing or using the information. Please contact the sender
immediately by return e-mail and delete the original message.

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Laboratory clerical job position *Delray Beach, FL*

[Delray Beach Pathology Kari Simeone via Histonet]

Hi Histonetters. I thought maybe I'd share a position we have available in our 
dermatology laboratory that is NOT TECHNICAL. Maybe you can share with someone 
who might be looking for a job in our area of Delray Beach, Florida. It is M-F 
9-5p, FULL TIME clerical work. 401k, benefits and vacation. 25k annually. 
Filing, phones, doctor interaction/assisting and data entry. Must take drug and 
personality tests along with a background check.



No experience necessary, WILL TRAIN. Please click on link to apply.



https://advancedderm.applicantpro.com/jobs/242606.html



Kari M Simeone

Histology/Immunohistochemistry Specialist Supervisor

Alternate Laboratory Supervisor

Delray Beach Technical Laboratory

ADCS Clinics, LLC



www.advancedderm.com





The information contained in this message and any attachments is intended only
for the use of the individual or entity to which it is addressed, and may 
contain
information that is privileged, confidential and exempt from disclosure under
applicable law. If you have received this message in error, you are prohibited
from copying, distributing or using the information. Please contact the sender
immediately by return e-mail and delete the original message.

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Radiation

[Fayad, Donna A. (BIDMC - Pathology) via Histonet]

Does anyone have a policy for a histotech who handles blocks of patients 
exposed to radiation prior to surgery?

Donna Fayad
Anatomical Pathology Manager
Beth Israel Deaconess Medical Center
330 Brookline Avenue
Boston, Massachusetts 02215

P 617-667-7831   F 617-17-975-5620
PAGER 93341






This message is intended for the use of the person(s) to whom it may be 
addressed. It may contain information that is privileged, confidential, or 
otherwise protected from disclosure under applicable law. If you are not the 
intended recipient, any dissemination, distribution, copying, or use of this 
information is prohibited. If you have received this message in error, please 
permanently delete it and immediately notify the sender. Thank you.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] CAP proficiency testing

[Nexgen Pathology via Histonet]

Hello all,
We are a histology lab and we are about to begin CAP proficiency testing in
order to acquire CAP certification. We currently have a menu of about 30
IHC antibodies that we currently run, including ER/PR/HER2 for breast.
The CAP proficiency test/surveys catalog has a pretty limited and somewhat
random menu of IHC antibodies that are available for testing.
Can anyone tell me what is the minimum IHC proficiency testing one needs to
do in order to comply with CAP certification?

Thanks much
Christina Pierre

*Nexgen Pathology*
6 Eastern Main Rd.
San Juan
Trinidad & Tobago
Tel./Fax. (868) 674-7284
nexgenpathology.com
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] Histonet Digest, Vol 144, Issue 14

[Nexgen Pathology via Histonet]

Hi, im a budding histotechnician at Nexgen Pathology Limited in Trinidad
and Tobago.
What are your protocols with respect to taking levels for biopsies? How
many levels do you usually take per biopsy?
Email me personally if you can @nicholas...@gmail.com

Thanks Alot
Nicholas Seepersad

*Nexgen Pathology*
6 Eastern Main Rd.
San Juan
Trinidad & Tobago
Tel./Fax. (868) 674-7284
nexgenpathology.com

On Sat, Nov 14, 2015 at 2:00 PM, <histonet-requ...@lists.utsouthwestern.edu>
wrote:

> Send Histonet mailing list submissions to
> histonet@lists.utsouthwestern.edu
>
> To subscribe or unsubscribe via the World Wide Web, visit
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> or, via email, send a message with subject or body 'help' to
> histonet-requ...@lists.utsouthwestern.edu
>
> You can reach the person managing the list at
> histonet-ow...@lists.utsouthwestern.edu
>
> When replying, please edit your Subject line so it is more specific
> than "Re: Contents of Histonet digest..."
>
>
> Today's Topics:
>
>1. Mohs sections (Angela Yepes)
>2. Re: IHC on old slides (Hobbs, Carl)
>3. Re: #ExtMail#  Slide and cassette printers (Ellenburg, Deborah)
>4. Histology Lab Survey Questions (Va Paula Sicurello)
>
>
> --
>
> Message: 1
> Date: Fri, 13 Nov 2015 13:23:56 -0500
> From: Angela Yepes <angelapatric...@gmail.com>
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] Mohs sections
> Message-ID: <9ab1bdcc-37ff-497d-a479-b394a8d5f...@gmail.com>
> Content-Type: text/plain;   charset=us-ascii
>
>
>
> > From: Angela Yepes <angelapatric...@gmail.com>
> > Date: November 12, 2015 at 6:05:57 PM EST
> > To: histonet@lists.utsouthwestern.edu
> > Subject: Mohs sections
> >
> >
> >> From: Angela Yepes <angelapatric...@gmail.com>
> >> Date: November 12, 2015 at 4:15:50 PM EST
> >> To: histonet-requ...@lists.utsouthwestern.edu
> >> Subject: Mohs sections
> >> Hello everyone:
> >> While cutting Sections for Mohs surgery I noticed the tissue detaching
> from the O.C.T. Embedding medium  and subsequent rolling of the tissue.
> >> To correct the problem the chuck was rotated to allow cutting from the
> opposite side (epidermis side). This maneuver did not correct the problem.
> I tried to unroll the edges with stiff brush which seemed to correct the
> problem, but it did not. I only  found out the sections were still rolling
> up after the staining.
> >> The cryostat temperature was -23C and the sections thickness was -14
> microns
> >> Please see pictures.
> >> Please help
> >>
> >>
> >>
> >>
> >> Sent from my iPhone
> >>
>
>
> --
>
> Message: 2
> Date: Fri, 13 Nov 2015 19:25:04 +
> From: "Hobbs, Carl" <carl.ho...@kcl.ac.uk>
> To: histonet <histonet@lists.utsouthwestern.edu>
> Subject: Re: [Histonet] IHC on old slides
> Message-ID:
> <
> vi1pr03mb1407c1c22a150f0a81dc6f04c4...@vi1pr03mb1407.eurprd03.prod.outlook.com
> >
>
> Content-Type: text/plain; charset="iso-8859-1"
>
> Amplification is the only way.
>
> Possibly, tyramide amplification will help.
>
> It will cost you to buy a kit...sure, make your own butyou have to
> weigh up pros/cons
>
>
> Sureyou will have to play with variables.
>
>
>
> --
>
> Message: 3
> Date: Fri, 13 Nov 2015 19:42:29 +
> From: "Ellenburg, Deborah" <deborah_ellenb...@bshsi.org>
> To: Mike Pence <mpe...@grhs.net>
> Cc: "histonet@lists.utsouthwestern.edu"
> <histonet@lists.utsouthwestern.edu>,
> "histonet-boun...@lists.utsouthwestern.edu"
> <histonet-boun...@lists.utsouthwestern.edu>
> Subject: Re: [Histonet] #ExtMail#  Slide and cassette printers
> Message-ID: <66c85b18-bce2-4f2d-9ff0-7136b605c...@bshsi.org>
> Content-Type: text/plain; charset="us-ascii"
>
> We have the ShurMark slide and cassette engraver that interfaces with
> CoPath.
>
> Sent from my iPhone
>
> > On Nov 12, 2015, at 10:50 AM, Mike Pence via Histonet <
> histonet@lists.utsouthwestern.edu> wrote:
> >
> > Okay, I am reaching out to the people that I know that has an answer to
> everything. What system are you using to print slides and cassettes from in
> your lab? Is it intergraded with your LIS and if so what LIS are you using?
> I am not looking for a system that print

[Histonet] FULLTIME DAY SHIFT (first shift) AND FULL TIME NIGHT SHIFT (IHC) POSITION DELRAY BCH FL

[Delray Beach Pathology Kari Simeone via Histonet]

Hi Histonetters! We are looking for TWO full time licensed histotechs here in 
our very busy Delray Beach, Florida dermatology laboratory. These are permanent 
full time, (40 hours) position with benefits (medical/401k/vacation). Annual 
salary in the $50k range (NIGHT SHIFT DIFF AVAILABLE). THIS IS A DRUG FREE 
WORKPLACE. Background check, personality test and drug test will be necessary. 
Sorry, no relocation assistance provided.


***PLEASE NO HEAD HUNTERS/PLACEMENT SERVICES***!!!

DAYTIME POSTION:
*full time position Mon-Fri 8a-5:30p (start time may vary)
*MUST be licensed as a FLORIDA HISTOTECHNICIAN OR HISTOTECHNOLOGIST (NO pending 
licensure pls)
*PREFER experience but WILL TRAIN a knowledgeable, willing candidate
*duties to include (but not limited to): grossing, microtomy, accessioning, 
embedding and general histology
*must be self motivated, reliable and a team player
DAYTIME SHIFT APPLICANT, PLEASE VISIT THIS LINK TO APPLY: 
https://advancedderm.applicantpro.com/jobs/240090.html

NIGHT POSITION:

*Full time position Mon-Fri 10:30p-7:00a (IMMUNOHISTOCHEMISTRY/SPECIALS 
department)

*MUST be licensed as a FLORIDA HISTOTECHNOLOGIST (this is NOT negotiable)

*EXPERIENCE WITH IHC A MUST! Leica (BOND) and Roche/Ventana equipment 
experience preferred

*Must be able to multi-task special stains

*MUST have at LEAST 2 years experience. Please DO NOT respond if you do not 
possess EXPERIENCE in this area!

*must be confidant, quick learner, self motivated, reliable and a team player

NIGHT SHIFT APPLICANT, PLEASE VISIT THIS LINK TO APPLY:

https://advancedderm.applicantpro.com/jobs/278020.html





Kari M Simeone
561.819.6517 fax
ksime...@leavittmgt.com







The information contained in this message and any attachments is intended only
for the use of the individual or entity to which it is addressed, and may 
contain
information that is privileged, confidential and exempt from disclosure under
applicable law. If you have received this message in error, you are prohibited
from copying, distributing or using the information. Please contact the sender
immediately by return e-mail and delete the original message.

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] FULLTIME DAY SHIFT (first shift) AND FULL TIME NIGHT SHIFT (IHC) POSITION DELRAY BCH FL

[Delray Beach Pathology Kari Simeone via Histonet]

Hi Histonetters! We are looking for TWO full time licensed histotechs here in 
our very busy Delray Beach, Florida dermatology laboratory. These are permanent 
full time, (40 hours) position with benefits (medical/401k/vacation). Annual 
salary in the $50k range (NIGHT SHIFT DIFF AVAILABLE). THIS IS A DRUG FREE 
WORKPLACE. Background check, personality test and drug test will be necessary. 
Sorry, no relocation assistance provided.


***PLEASE NO HEAD HUNTERS/PLACEMENT SERVICES***!!!

DAYTIME POSTION:
*full time position Mon-Fri 8a-5:30p (start time may vary)
*MUST be licensed as a FLORIDA HISTOTECHNICIAN OR HISTOTECHNOLOGIST (NO pending 
licensure pls)
*PREFER experience but WILL TRAIN a knowledgeable, willing candidate
*duties to include (but not limited to): grossing, microtomy, accessioning, 
embedding and general histology
*must be self motivated, reliable and a team player
DAYTIME SHIFT APPLICANT, PLEASE VISIT THIS LINK TO APPLY: 
https://advancedderm.applicantpro.com/jobs/240090.html

NIGHT POSITION:

*Full time position Mon-Fri 10:30p-7:00a (IMMUNOHISTOCHEMISTRY/SPECIALS 
department)

*MUST be licensed as a FLORIDA HISTOTECHNOLOGIST (this is NOT negotiable)

*EXPERIENCE WITH IHC A MUST! Leica (BOND) and Roche/Ventana equipment 
experience preferred

*Must be able to multi-task special stains

*MUST have at LEAST 2 years experience. Please DO NOT respond if you do not 
possess EXPERIENCE in this area!

*must be confidant, quick learner, self motivated, reliable and a team player

NIGHT SHIFT APPLICANT, PLEASE VISIT THIS LINK TO APPLY:

https://advancedderm.applicantpro.com/jobs/278020.html





Kari M Simeone
561.819.6517 fax
ksime...@leavittmgt.com







The information contained in this message and any attachments is intended only
for the use of the individual or entity to which it is addressed, and may 
contain
information that is privileged, confidential and exempt from disclosure under
applicable law. If you have received this message in error, you are prohibited
from copying, distributing or using the information. Please contact the sender
immediately by return e-mail and delete the original message.

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] Microtomy statisitcs (White, Lisa M.)

[Delray Beach Pathology Kari Simeone via Histonet]

We timed about 5 techs cutting multiple blocks (shaves/excisions) and made an 
average from those times. Not everyone cuts at the same speed and technique 
varies. An 'average' range is what our techs are expected to fall into. 

Kari M Simeone
Histology/Immunohistochemistry Specialist Supervisor
Alternate Laboratory Supervisor
Delray Beach Technical Laboratory
ADCS Clinics, LLC


The information contained in this message and any attachments is intended only
for the use of the individual or entity to which it is addressed, and may 
contain
information that is privileged, confidential and exempt from disclosure under
applicable law. If you have received this message in error, you are prohibited
from copying, distributing or using the information. Please contact the sender
immediately by return e-mail and delete the original message.

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] FULLTIME DAY SHIFT (first shift) AND FULL TIME NIGHT SHIFT (IHC) POSITION DELRAY BCH FL

[Delray Beach Pathology Kari Simeone via Histonet]

Hi Histonetters! We are looking for TWO full time licensed histotechs here in 
our very busy Delray Beach, Florida dermatology laboratory. These are permanent 
full time, (40 hours) position with benefits (medical/401k/vacation). Annual 
salary in the $50k range (NIGHT SHIFT DIFF AVAILABLE). THIS IS A DRUG FREE 
WORKPLACE. Background check, personality test and drug test will be necessary. 
Sorry, no relocation assistance provided.


***PLEASE NO HEAD HUNTERS/PLACEMENT SERVICES***!!!

DAYTIME POSTION:
*full time position Mon-Fri 8a-5:30p (start time may vary)
*MUST be licensed as a FLORIDA HISTOTECHNICIAN OR HISTOTECHNOLOGIST (NO pending 
licensure pls)
*PREFER experience but WILL TRAIN a knowledgeable, willing candidate
*duties to include (but not limited to): grossing, microtomy, accessioning, 
embedding and general histology
*must be self motivated, reliable and a team player
DAYTIME SHIFT APPLICANT, PLEASE VISIT THIS LINK TO APPLY: 
https://advancedderm.applicantpro.com/jobs/240090.html

NIGHT POSITION:

*Full time position Mon-Fri 10:30p-7:00a (IMMUNOHISTOCHEMISTRY/SPECIALS 
department)

*MUST be licensed as a FLORIDA HISTOTECHNOLOGIST (this is NOT negotiable)

*EXPERIENCE WITH IHC A MUST! Leica (BOND) and Roche/Ventana equipment 
experience preferred

*Must be able to multi-task special stains

*MUST have at LEAST 2 years experience. Please DO NOT respond if you do not 
possess EXPERIENCE in this area!

*must be confidant, quick learner, self motivated, reliable and a team player

NIGHT SHIFT APPLICANT, PLEASE VISIT THIS LINK TO APPLY:

https://advancedderm.applicantpro.com/jobs/278020.html





Kari M Simeone
561.819.6517 fax
ksime...@leavittmgt.com







The information contained in this message and any attachments is intended only
for the use of the individual or entity to which it is addressed, and may 
contain
information that is privileged, confidential and exempt from disclosure under
applicable law. If you have received this message in error, you are prohibited
from copying, distributing or using the information. Please contact the sender
immediately by return e-mail and delete the original message.

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] FULLTIME DAY SHIFT (first shift) POSITION DELRAY BCH FL

[Delray Beach Pathology Kari Simeone via Histonet]

Hi Histonetters! We are looking for a full time licensed histotech here in our 
very busy Delray Beach, Florida dermatology laboratory. This is a permanent 
full time, DAYTIME SHIFT (40 hours) position with benefits 
(medical/401k/vacation). Annual salary in the $50k range. THIS IS A DRUG FREE 
WORKPLACE. Background check, personality test and drug test will be necessary. 
Sorry, no relocation assistance provided.


***PLEASE NO HEAD HUNTERS/PLACEMENT SERVICES***!!!

PLEASE VISIT THIS LINK TO APPLY: 
https://advancedderm.applicantpro.com/jobs/240090.html

*full time position Mon-Fri 8a-5:30p (start time may vary)
*MUST be licensed as a FLORIDA HISTOTECHNICIAN OR HISTOTECHNOLOGIST (NO pending 
licensure pls)
*PREFER experience but WILL TRAIN a knowledgeable, willing candidate
*duties to include (but not limited to): grossing, microtomy, accessioning, 
embedding and general histology
*must be self motivated, reliable and a team player


Kari M Simeone
561.819.6517 fax
ksime...@leavittmgt.com







The information contained in this message and any attachments is intended only
for the use of the individual or entity to which it is addressed, and may 
contain
information that is privileged, confidential and exempt from disclosure under
applicable law. If you have received this message in error, you are prohibited
from copying, distributing or using the information. Please contact the sender
immediately by return e-mail and delete the original message.

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] FULLTIME DAY SHIFT (first shift) POSITION DELRAY BCH FL

[Delray Beach Pathology Kari Simeone via Histonet]

Hi Histonetters! We are looking for a full time licensed histotech here in our 
very busy Delray Beach, Florida dermatology laboratory. This is a permanent 
full time, DAYTIME SHIFT (40 hours) position with benefits 
(medical/401k/vacation). Annual salary in the $50k range. THIS IS A DRUG FREE 
WORKPLACE. Background check, personality test and drug test will be necessary. 
Sorry, no relocation assistance provided.


***PLEASE NO HEAD HUNTERS/PLACEMENT SERVICES***WE DO NOT HIRE TEMPS!!***

PLEASE VISIT THIS LINK TO APPLY: 
https://advancedderm.applicantpro.com/jobs/240090.html

*full time position Mon-Fri 8a-5:30p (start time may vary)
*MUST be licensed as a FLORIDA HISTOTECHNICIAN OR HISTOTECHNOLOGIST (NO pending 
licensure pls)
*PREFER experience but WILL TRAIN a knowledgeable, willing candidate
*duties to include (but not limited to): grossing, microtomy, accessioning, 
embedding and general histology
*must be self motivated, reliable and a team player


Kari M Simeone
561.819.6517 fax
ksime...@leavittmgt.com







The information contained in this message and any attachments is intended only
for the use of the individual or entity to which it is addressed, and may 
contain
information that is privileged, confidential and exempt from disclosure under
applicable law. If you have received this message in error, you are prohibited
from copying, distributing or using the information. Please contact the sender
immediately by return e-mail and delete the original message.

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] FULLTIME DAY SHIFT (first shift) POSITION DELRAY BCH FL

[Delray Beach Pathology Kari Simeone via Histonet]

Hi Histonetters! We are looking for a full time licensed histotech here in our 
very busy Delray Beach, Florida dermatology laboratory. This is a permanent 
full time, DAYTIME SHIFT (40 hours) position with benefits 
(medical/401k/vacation). Annual salary in the $50k range. THIS IS A DRUG FREE 
WORKPLACE. Background check, personality test and drug test will be necessary. 
Sorry, no relocation assistance provided.


***PLEASE NO HEAD HUNTERS/PLACEMENT SERVICES***!!!

PLEASE VISIT THIS LINK TO APPLY: 
https://advancedderm.applicantpro.com/jobs/240090.html

*full time position Mon-Fri 8a-5:30p (start time may vary)
*MUST be licensed as a FLORIDA HISTOTECHNICIAN OR HISTOTECHNOLOGIST (NO pending 
licensure pls)
*PREFER experience but WILL TRAIN a knowledgeable, willing candidate
*duties to include (but not limited to): grossing, microtomy, accessioning, 
embedding and general histology
*must be self motivated, reliable and a team player


Kari M Simeone
561.819.6517 fax
ksime...@leavittmgt.commailto:ksime...@leavittmgt.com







The information contained in this message and any attachments is intended only
for the use of the individual or entity to which it is addressed, and may 
contain
information that is privileged, confidential and exempt from disclosure under
applicable law. If you have received this message in error, you are prohibited
from copying, distributing or using the information. Please contact the sender
immediately by return e-mail and delete the original message.

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] FT NIGHT (evening shift) POSITION DELRAY BCH FL

[Delray Beach Pathology Kari Simeone via Histonet]

Hi Histonetters! We are looking for a full time licensed histotech here in our 
very busy Delray Florida Dermatology Lab. This is a permanent full time NIGHT 
SHIFT (40 hours) position with benefits (medical/401k/vacation) and shift 
differential. THIS IS A DRUG FREE WORKPLACE. Background check, personality test 
and drug test will be necessary. Sorry, no relocation assistance provided.


***PLEASE NO HEAD HUNTERS/PLACEMENT SERVICES***!!!

Email your resume to lengim...@leavittmgt.commailto:lengim...@leavittmgt.com 
if interested.

*full time position Mon-Fri or Sun-Thurs 6PM-2:30AM
*MUST be licensed as a FL HISTOTEHCNOLOGIST ONLY (will be working solo half of 
your shift)
*MUST have at LEAST 2 years experience (dermatology preferred) Please DO NOT 
respond if no EXPERIENCE!
*VERY proficient in embedding and microtomy
*must be self motivated, reliable and a team player
*knowledge in operating Ventana and Leica equipment desired (not necessary)
*some IHC experience preferred

Kari M Simeone
561.819.6517 fax
ksime...@leavittmgt.commailto:ksime...@leavittmgt.com







The information contained in this message and any attachments is intended only
for the use of the individual or entity to which it is addressed, and may 
contain
information that is privileged, confidential and exempt from disclosure under
applicable law. If you have received this message in error, you are prohibited
from copying, distributing or using the information. Please contact the sender
immediately by return e-mail and delete the original message.

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] FT NIGHT (evening shift) POSITION DELRAY BCH FL

[Delray Beach Pathology Kari Simeone via Histonet]

Hi Histonetters! We are looking for a full time licensed histotech here in our 
very busy Delray Florida Dermatology Lab. This is a permanent full time NIGHT 
SHIFT (40 hours) position with benefits (medical/401k/vacation) and shift 
differential. THIS IS A DRUG FREE WORKPLACE. Background check, personality test 
and drug test will be necessary. Sorry, no relocation assistance provided.


***PLEASE NO HEAD HUNTERS/PLACEMENT SERVICES***!!!

Email your resume to lengim...@leavittmgt.commailto:lengim...@leavittmgt.com 
if interested.

*full time position Mon-Fri or Sun-Thurs 6PM-2:30AM
*MUST be licensed as a FL HISTOTEHCNOLOGIST ONLY (will be working solo half of 
your shift)
*MUST have at LEAST 2 years experience (dermatology preferred) Please DO NOT 
respond if no EXPERIENCE!
*VERY proficient in embedding and microtomy
*must be self motivated, reliable and a team player
*knowledge in operating Ventana and Leica equipment desired (not necessary)
*some IHC experience preferred

Kari M Simeone
561.819.6517 fax
ksime...@leavittmgt.commailto:ksime...@leavittmgt.com







The information contained in this message and any attachments is intended only
for the use of the individual or entity to which it is addressed, and may 
contain
information that is privileged, confidential and exempt from disclosure under
applicable law. If you have received this message in error, you are prohibited
from copying, distributing or using the information. Please contact the sender
immediately by return e-mail and delete the original message.

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] FT EVENING (3rd shift) 6p-2a POSITION DELRAY BCH FL

[Delray Beach Pathology Kari Simeone]

Hi Histonetters! We are looking for a full time licensed histotech here in our 
very busy Delray Florida Dermatology Lab. This is a permanent full time EVENING 
SHIFT (40 hours) position with benefits (medical/401k/vacation) and shift 
differential. THIS IS A DRUG FREE WORKPLACE. Background check, personality 
assessment and drug test will be necessary. Sorry, no relocation assistance 
provided.


***PLEASE NO HEAD HUNTERS/PLACEMENT SERVICES***!!!

Email your resume to lengim...@leavittmgt.commailto:lengim...@leavittmgt.com 
if interested.

*full time position Mon-Fri or Sun-Thurs 6PM-2:30AM
*MUST be licensed as a FL HISTOTEHCNOLOGIST ONLY (will be working solo half of 
your shift)
*MUST have at LEAST 2 years experience (dermatology preferred) Please DO NOT 
respond if no EXPERIENCE!
*VERY proficient in embedding and microtomy
*must be self motivated, reliable and a team player
*knowledge in operating Ventana and Leica equipment desired (not necessary)
*some IHC experience preferred

Kari M Simeone
561.819.6517 fax
ksime...@leavittmgt.commailto:ksime...@leavittmgt.com







The information contained in this message and any attachments is intended only
for the use of the individual or entity to which it is addressed, and may 
contain
information that is privileged, confidential and exempt from disclosure under
applicable law. If you have received this message in error, you are prohibited
from copying, distributing or using the information. Please contact the sender
immediately by return e-mail and delete the original message.

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] FT NIGHT (3rd shift) POSITION DELRAY BCH FL

[Delray Beach Pathology Kari Simeone]

Hi Histonetters! We are looking for a full time licensed histotech here in our 
very busy Delray Florida Dermatology Lab. This is a permanent full time NIGHT 
SHIFT (40 hours) position with benefits (medical/401k/vacation) and shift 
differential. THIS IS A DRUG FREE WORKPLACE. Background check, personality test 
and drug test will be necessary. Sorry, no relocation assistance provided.


***PLEASE NO HEAD HUNTERS/PLACEMENT SERVICES***!!!

Email your resume to lengim...@leavittmgt.commailto:lengim...@leavittmgt.com 
if interested.

*full time position Mon-Fri or Sun-Thurs 6PM-2:30AM
*MUST be licensed as a FL HISTOTEHCNOLOGIST ONLY (will be working solo half of 
your shift)
*MUST have at LEAST 2 years experience (dermatology preferred) Please DO NOT 
respond if no EXPERIENCE!
*VERY proficient in embedding and microtomy
*must be self motivated, reliable and a team player
*knowledge in operating Ventana and Leica equipment desired (not necessary)
*some IHC experience preferred

Kari M Simeone
561.819.6517 fax
ksime...@leavittmgt.commailto:ksime...@leavittmgt.com







The information contained in this message and any attachments is intended only
for the use of the individual or entity to which it is addressed, and may 
contain
information that is privileged, confidential and exempt from disclosure under
applicable law. If you have received this message in error, you are prohibited
from copying, distributing or using the information. Please contact the sender
immediately by return e-mail and delete the original message.

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] FT position HISTOTECHNOLOGIST/IHC Delray Beach, FL

[Delray Beach Pathology Kari Simeone]

Hi Histonetters! We are looking for a full time licensed histotech here in our 
very busy Delray Florida Dermatology Lab. This is a permanent full time SECOND 
SHIFT (40 hours) position with benefits (medical/401k/vacation) and competitive 
pay. THIS IS A DRUG FREE WORKPLACE. Drug testing, background check and 
personality testing is required. ONLY SERIOUS INQURIES, please read EVERY 
qualification desired. Sorry, NO relocation assistance offered. Position 
available for training immediately.



***PLEASE NO HEAD HUNTERS/PLACEMENT SERVICES***!!!



Email your resume to lengim...@leavittmgt.commailto:lengim...@leavittmgt.com 
if interested.





*Full time position Mon-Fri 2p-10:30p (IMMUNOHISTOCHEMISTRY/SPECIALS department)

*MUST be licensed as a FLORIDA HISTOTECHNOLOGIST (this is NOT negotiable)

*EXPERIENCE WITH IHC A MUST! Leica (BOND) and Roche/Ventana equipment 
experience preferred

*Must be able to multi-task special stains

*MUST have at LEAST 2 years experience. Please DO NOT respond if you do not 
possess EXPERIENCE in this area!

*must be confidant, quick learner, self motivated, reliable and a team player





Kari M Simeone

Histology/Immunohistochemistry Specialist Supervisor









The information contained in this message and any attachments is intended only
for the use of the individual or entity to which it is addressed, and may 
contain
information that is privileged, confidential and exempt from disclosure under
applicable law. If you have received this message in error, you are prohibited
from copying, distributing or using the information. Please contact the sender
immediately by return e-mail and delete the original message.

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] FT NIGHT (3rd shift) POSITION DELRAY BCH FL

[Delray Beach Pathology Kari Simeone]

Hi Histonetters! We are looking for a full time licensed histotech here in our 
very busy Delray Florida Dermatology Lab. This is a permanent full time NIGHT 
SHIFT (40 hours) position with benefits (medical/401k/vacation) and shift 
differential. THIS IS A DRUG FREE WORKPLACE. Background check, personality test 
and drug test will be necessary. Sorry, no relocation assistance provided.


***PLEASE NO HEAD HUNTERS/PLACEMENT SERVICES***!!!

Email your resume to lengim...@leavittmgt.commailto:lengim...@leavittmgt.com 
if interested.

*full time position Mon-Fri or Sun-Thurs 6PM-2:30AM
*MUST be licensed as a FL HISTOTEHCNOLOGIST ONLY (will be working solo)
*MUST have at LEAST 2 years experience (dermatology preferred) Please DO NOT 
respond if no EXPERIENCE!
*VERY proficient in embedding and microtomy
*must be self motivated, reliable and a team player
*knowledge in operating Ventana and Leica equipment desired (not necessary)
*some IHC experience preferred

Kari M Simeone
561.819.6517 fax
ksime...@leavittmgt.commailto:ksime...@leavittmgt.com







The information contained in this message and any attachments is intended only
for the use of the individual or entity to which it is addressed, and may 
contain
information that is privileged, confidential and exempt from disclosure under
applicable law. If you have received this message in error, you are prohibited
from copying, distributing or using the information. Please contact the sender
immediately by return e-mail and delete the original message.

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] FT position HISTOTECHNOLOGIST/IHC Delray Beach, FL

[Delray Beach Pathology Kari Simeone]

Hi Histonetters! We are looking for a full time licensed histotech here in our 
very busy Delray Florida Dermatology Lab. This is a permanent full time SECOND 
SHIFT (40 hours) position with benefits (medical/401k/vacation) and competitive 
pay. THIS IS A DRUG FREE WORKPLACE. Drug testing, background check and 
personality testing is required. ONLY SERIOUS INQURIES, please read EVERY 
qualification desired. Sorry, NO relocation assistance offered. Position 
available for training immediately.



***PLEASE NO HEAD HUNTERS/PLACEMENT SERVICES***!!!



Email your resume to lengim...@leavittmgt.commailto:lengim...@leavittmgt.com 
if interested.





*Full time position Mon-Fri 2p-10:30p (IMMUNOHISTOCHEMISTRY/SPECIALS department)

*MUST be licensed as a FLORIDA HISTOTECHNOLOGIST (this is NOT negotiable)

*EXPERIENCE WITH IHC A MUST! Leica (BOND) and Roche/Ventana equipment 
experience preferred

*Must be able to multi-task special stains

*MUST have at LEAST 2 years experience. Please DO NOT respond if you do not 
possess EXPERIENCE in this area!

*must be confidant, quick learner, self motivated, reliable and a team player





Kari M Simeone

Histology/Immunohistochemistry Specialist Supervisor







The information contained in this message and any attachments is intended only 
for the use of the individual or entity to which it is addressed, and may 
contain information that is privileged, confidential and exempt from disclosure 
under applicable law. If you have received this message in error, you are 
prohibited from copying, distributing or using the information. Please contact 
the sender immediately by return e-mail and delete the original message.


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] Billing

[Martha Ward-Pathology]

Yes that is how we do it as well.



 
Martha Ward, MT (ASCP) QIHC
Manager

Molecular Diagnostics Lab
Medical Center Boulevard  \  Winston-Salem, NC 27157
p 336.716.2109  \  f 336.716.5890  
mw...@wakehealth.edu  
 
 



-Original Message-
From: Vickroy, James [mailto:jvick...@springfieldclinic.com] 
Sent: Friday, May 29, 2015 3:13 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Billing


Just need a reminder:

If a pathologist orders the same  several IHC stains on two blocks from the 
same specimen am I correct to think that we can only charge the stains on one 
of the blocks? 88342 and the remainder 88341's

Jim

Jim Vickroy
Histology Manager
Springfield Clinic, Main Campus, East Building
1025 South 6th Street
Springfield, Illinois  62703
Office:  217-528-7541, Ext. 15121
Email:  jvick...@springfieldclinic.commailto:jvick...@springfieldclinic.com



This electronic message contains information from Springfield Clinic, LLP that 
may be confidential, privileged, and/or sensitive. This information is intended 
for the use of the individual(s) or entity(ies) named above. If you are not the 
intended recipient, be aware that disclosure, copying, distribution, or action 
taken on the contents of this information is strictly prohibited. If you have 
received this electronic message in error, please notify the sender 
immediately, by electronic mail, so that arrangements may be made for the 
retrieval of this electronic message. Thank you.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] IHC billing question

[Martha Ward-Pathology]

That's what we do as well.   

Martha Ward
Wake Forest Baptist Health

From: Weems, Joyce K. [joyce.we...@emoryhealthcare.org]
Sent: Friday, May 01, 2015 9:13 AM
To: 'Cartun, Richard'; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] IHC billing question

I do it every day  - change every first to 88342.

Joyce Weems
Pathology Manager
678-843-7376 Phone
678-843-7831 Fax
joyce.we...@emoryhealthcare.org



www.saintjosephsatlanta.org
5665 Peachtree Dunwoody Road
Atlanta, GA 30342

This e-mail, including any attachments is the property of Saint Joseph's 
Hospital and is intended for the sole use of the intended recipient(s).  It may 
contain information that is privileged and confidential.  Any unauthorized 
review, use, disclosure, or distribution is prohibited. If you are not the 
intended recipient, please delete this message, and reply to the sender 
regarding the error in a separate email.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cartun, Richard
Sent: Thursday, April 30, 2015 5:33 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] IHC billing question

Effective January 1, 2015, our LIS team removed all of the CPT 88342 codes for 
IHC from our CoPath stain dictionary since you couldn't tell whether a 
Cytokeratin-7 was being performed as an 88341 or as an 88342.  Now, as you 
might have expected,  none of the inpatient IHC testing has been accounted 
for (the outpatient IHC has been billed manually from the pathology report), 
and they want someone to go back and enter all the CPT codes into the system 
(hopefully, not me!).  Has anyone else encountered this problem?  Thanks (I 
think).

Richard

Richard W. Cartun, MS, PhD
Director, Histology  Immunopathology
Director, Biospecimen Collection Programs Assistant Director, Anatomic 
Pathology Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 972-1596
(860) 545-2204 Fax


This e-mail message, including any attachments, is for the sole use of the 
intended recipient(s) and may contain confidential and privileged information. 
Any unauthorized review, use, disclosure, or distribution is prohibited. If you 
are not the intended recipient, or an employee or agent responsible for 
delivering the message to the intended recipient, please contact the sender by 
reply e-mail and destroy all copies of the original message, including any 
attachments.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



This e-mail message (including any attachments) is for the sole use of
the intended recipient(s) and may contain confidential and privileged
information. If the reader of this message is not the intended
recipient, you are hereby notified that any dissemination, distribution
or copying of this message (including any attachments) is strictly
prohibited.

If you have received this message in error, please contact
the sender by reply e-mail message and destroy all copies of the
original message (including attachments).

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] RE: IHC Billing Question

[Martha Ward-Pathology]

We have not been charging for the negative control, assuming that it was just a 
cost of doing business.  I would be interested to hear if anyone has been 
charging for their negative controls as well.
 
Martha Ward, MT (ASCP) QIHC
Manager

Molecular Diagnostics Lab
Medical Center Boulevard  \  Winston-Salem, NC 27157
p 336.716.2109  \  f 336.716.5890  
mw...@wakehealth.edu  
 
 



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Burnett, Brandy
Sent: Thursday, April 16, 2015 9:48 AM
To: 'Joana Moreira'; histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: IHC Billing Question 

We recently added HER2 IHC testing in our lab which we are required to use a 
negative reagent control For each case. Is there a cpt code for negative 
reagent control reimbursement? Any information on this Would be much 
appreciated!
Thanks

Brandy Burnett
Histotechnoligist, QIHC(ASCP)
CCH Pathology/Histology


Expert physicians. Quality hospitals. Superior care. 


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Joana Moreira
Sent: Thursday, April 16, 2015 7:25 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] IHC Billing Question 

Greetings from Doha!

This was much probably discussed before, but I was wondering if you could help 
me with a query in regards to billing.
For the sites that are still doing an IHC negative reagent control for each 
patient specimen, do you bill for the negative control? Using code 88341?

I believe it should be billed (since when and if performed correctly the 
negative control follows a normal IHC technique) however I am completely new to 
the billing subject. My previous experience is based in Portugal and UK (where 
billing does not exist) and I've been introduced to this topic since I joined 
my current institution that will be following the North American Healthcare 
model. So... any help will be GREATLY appreciated!!

Many Thanks in advance,
Joana

Joana Moreira
Supervisor - Anatomical Pathology
Department of Pathology

Sidra Medical  Research Center
PO Box 26999 | Doha, Qatar
Direct Line  +974-4404-2036
jmore...@sidra.org | www.sidra.org





Disclaimer: This email and its attachments may be confidential and are intended 
solely for the use of the individual to whom it is addressed. If you are not 
the intended recipient, any reading, printing, storage, disclosure, copying or 
any other action taken in respect of this e-mail is prohibited and may be 
unlawful. If you are not the intended recipient, please notify the sender 
immediately by using the reply function and then permanently delete what you 
have received. Any views or opinions expressed are solely those of the author 
and do not necessarily represent those of Sidra Medical and Research Center.

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

~~
This email and any files transmitted with it are confidential, and intended 
solely for the use of the individual or entity to whom they are addressed. If 
you have received this email in error  contact the Help Desk for Cape Cod 
Healthcare.

helpd...@capecodhealth.org

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] FULL TIME POSITION DELRAY BEACH, FL

[Delray Beach Pathology Kari Simeone]

Hi Histonetters! We are looking for a full time licensed histotech here in our 
very busy Delray Florida Dermatology Lab. This is a permanent full time SECOND 
SHIFT (40 hours) position with benefits (medical/401k/vacation) and competitive 
pay. THIS IS A DRUG FREE WORKPLACE.

ONLY SERIOUS INQURIES, please read EVERY qualification desired.



***PLEASE NO HEAD HUNTERS/PLACEMENT SERVICES***!!!



Email your resume to lengim...@leavittmgt.commailto:lengim...@leavittmgt.com 
if interested.



*Full time position Mon-Fri 12:30p-9p (general tech duties to include grossing, 
microtomy, embedding, etc)

*MUST be licensed as a FL histotechnologist or technician

*will train but you MUST be open and willing to learn and absorb the routine 
quickly

*must be self motivated, reliable and a team player



Kari M Simeone



561.819.6517 fax

ksime...@leavittmgt.commailto:ksime...@leavittmgt.com



The information contained in this message and any attachments is intended only 
for the use of the individual or entity to which it is addressed, and may 
contain information that is privileged, confidential and exempt from disclosure 
under applicable law. If you have received this message in error, you are 
prohibited from copying, distributing or using the information. Please contact 
the sender immediately by return e-mail and delete the original message.


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] OPEN POSITIONS IN DELRAY, FL LAB

[Delray Beach Pathology Kari Simeone]

Hi Histonetters! We are looking for (2) full time licensed histotechs here in 
our very busy Delray Florida Dermatology Lab. These are permanent full time 
SECOND SHIFT (40 hours) positions with benefits (medical/401k/vacation) and 
competitive pay. THIS IS A DRUG FREE WORKPLACE.

ONLY SERIOUS INQURIES, please read EVERY qualification desired.



***PLEASE NO HEAD HUNTERS/PLACEMENT SERVICES***!!!



Email your resume to lengim...@leavittmgt.commailto:lengim...@leavittmgt.com 
if interested.



*FIRST full time position Mon-Fri 12:30p-9p (general tech duties to include 
grossing, microtomy, embedding, etc)

*MUST be licensed as a FL histotechnologist or technician

*will train but you MUST be open and willing to learn and absorb the routine 
quickly

*must be self motivated, reliable and a team player



*SECOND full time position Mon-Fri 3p-11:30p (IMMUNOHISTOCHEMISTRY/SPECIALS 
department)

*MUST be licensed as a FL HISTOTECHNOLOGIST

*EXPERIENCE WITH IHC A MUST! Leica (BOND) and Roche/Ventana equipment 
experience preferred

*MUST have at LEAST 2 years experience. Please DO NOT respond if no EXPERIENCE!

*must be confidant, self motivated, reliable and a team player



Kari M Simeone

561.819.6517 fax

ksime...@leavittmgt.commailto:ksime...@leavittmgt.com





The information contained in this message and any attachments is intended only 
for the use of the individual or entity to which it is addressed, and may 
contain information that is privileged, confidential and exempt from disclosure 
under applicable law. If you have received this message in error, you are 
prohibited from copying, distributing or using the information. Please contact 
the sender immediately by return e-mail and delete the original message.


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Her2 IHC validation

[Martha Ward-Pathology]

In advance of preparing for our CAP inspection window, I am working on our 
validation write-up for our Her2 IHC and was looking for some data concerning 
the correlation rates.We compared our IHC staining to FISH Her2 results on 
the same case.  Is there a minimum correlation rate?   I have been unable to 
find one in my reading.   Thank you in advance for your help.


 
Martha Ward, MT (ASCP) QIHC
Manager

Molecular Diagnostics Lab
Medical Center Boulevard  \  Winston-Salem, NC 27157
p 336.716.2109  \  f 336.716.5890  
mw...@wakehealth.edu  
 
 


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

RE: [Histonet] p16 Antibody

[Martha Ward-Pathology]

We dilute ours 1:5 from the predilute and run it on the Bond 3.   It works very 
well.

 
Martha Ward, MT (ASCP) QIHC
Manager

Molecular Diagnostics Lab
Medical Center Boulevard  \  Winston-Salem, NC 27157
p 336.716.2109  \  f 336.716.5890  
mw...@wakehealth.edu  
 
 



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Burnett, Brandy
Sent: Wednesday, January 14, 2015 10:15 AM
To: 'sarah.dys...@stdavids.com'; lseb...@uwhealth.org; 
veronique.bar...@gmail.com; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] p16 Antibody

We use the Ventana (INK4a) clone as well. What dilution do you use?

Brandy Burnett
Histotechnoligist, QIHC(ASCP)
CCH Pathology/Histology
508-862-5267
bburn...@capecodhealth.org


Expert physicians. Quality hospitals. Superior care. 


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of 
sarah.dys...@stdavids.com
Sent: Wednesday, January 14, 2015 10:01 AM
To: lseb...@uwhealth.org; veronique.bar...@gmail.com; 
histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] p16 Antibody

Ventana unfortunately has the market on the good clone...you can dilute it 
out pretty far (it comes pre-diluted) and still get good results however...


Sarah Dysart  BA, HT (ASCP), QIHC (ASCP) Pathology Supervisor North Austin 
Medical Center
12221 North Mopac Expressway
Austin, TX  78758
512-901-1220 (lab)
512-901-6659 (office)



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sebree Linda A
Sent: Wednesday, January 14, 2015 8:41 AM
To: 'Véronique Barrès'; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] p16 Antibody

We use one from BD, Veronique, with good results. 

Linda A. Sebree
University of Wisconsin Hospital  Clinics IHC/ISH Laboratory, Rm A4/204-3224
600 Highland Ave. 
Madison, WI 53792
(608)265-6596
FAX: (608)262-7174 


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Véronique Barrès
Sent: Wednesday, January 14, 2015 8:06 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] p16 Antibody

Hi Histonetters!

I am working in a research lab and we sometimes need to do p16 staining, but 
our pathologist told us that the antibody we are using right now is not good. 
I'd like to know which antibody you're using in your labs?

Thanks for your help and happy Wednesday!
Véronique
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

~~
This email and any files transmitted with it are confidential, and intended 
solely for the use of the individual or entity to whom they are addressed. If 
you have received this email in error  contact the Help Desk for Cape Cod 
Healthcare.

helpd...@capecodhealth.org
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] back orders from various vendors

[Martha Ward-Pathology]

I just need a moment to ventis it just me or is everyone seeing more and 
more back order issues with their various vendors?   It is not specific to any 
one particular vendor, product or even category of product, but lately I am 
being told more and more often that an item is not availableexpected 
release dates anywhere from a few days to 6-8 weeks!  This is starting to 
cause real problems .

Thanks for letting get this off my chest.I will resume my frantic search 
for another antibody vendor now. 

 
Martha Ward, MT (ASCP) QIHC
Manager
 
Molecular Diagnostics Lab
Medical Center Boulevard  \  Winston-Salem, NC 27157
p 336.716.2109  \  f 336.716.5890  
mw...@wakehealth.edu  





___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] RE: back orders from various vendors

[Martha Ward-Pathology]

At least I know I am not crazy or being paranoid!   :) this year just seems 
to be worse than I ever remember it beingand the worse part is while I am 
freaking out because I can't get something I desperately need, the customer 
service person can only say  We are sorry for the inconvenience.   
Argg



-Original Message-
From: Marcum, Pamela A [mailto:pamar...@uams.edu] 
Sent: Tuesday, December 02, 2014 10:59 AM
To: Martha Ward-Pathology; histonet@lists.utsouthwestern.edu
Subject: RE: back orders from various vendors

Happens every year at this time with various vendors and end of year.  I always 
over order in October to be sure I am good through the end of the year or as 
close as I can.  I know the things I use most and those are just what is always 
in backorder at the end of the year.  October became my answer as long as 
expiration dates are not an issue.

Pam Marcum
UAMS

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Martha 
Ward-Pathology
Sent: Tuesday, December 02, 2014 9:48 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] back orders from various vendors


I just need a moment to ventis it just me or is everyone seeing more and 
more back order issues with their various vendors?   It is not specific to any 
one particular vendor, product or even category of product, but lately I am 
being told more and more often that an item is not availableexpected 
release dates anywhere from a few days to 6-8 weeks!  This is starting to 
cause real problems .

Thanks for letting get this off my chest.I will resume my frantic search 
for another antibody vendor now. 

 
Martha Ward, MT (ASCP) QIHC
Manager
 
Molecular Diagnostics Lab
Medical Center Boulevard  \  Winston-Salem, NC 27157 p 336.716.2109  \  f 
336.716.5890 mw...@wakehealth.edu  





___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

--
Confidentiality Notice: This e-mail message, including any attachments, is for 
the sole use of the intended recipient(s) and may contain confidential and 
privileged information. Any unauthorized review, use, disclosure or 
distribution is prohibited. If you are not the intended recipient, please 
contact the sender by reply e-mail and destroy all copies of the original 
message.


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] RE: EDTA decalcification solution follow up questions

[Martha Ward-Pathology]

Thank you to everyone who has responded.   They have a more specific question:


Currently they are using SurgiPath DeCal solution II. They are being asked 
to use an EDTA decal solution that will provide the best preservation of RNA 
and DNA proteins.   What is everyone using for this purpose?

Thanks again for any information


 
Martha Ward, MT (ASCP) QIHC
Manager

Molecular Diagnostics Lab
Medical Center Boulevard  \  Winston-Salem, NC 27157
p 336.716.2109  \  f 336.716.5890  
mw...@wakehealth.edu  
 
 





-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Martha 
Ward-Pathology
Sent: Monday, December 01, 2014 2:21 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] EDTA decalcification solution

I am asking this question for our Histology Lab.  They are being asked about 
using EDTA as a decal solution for bone and wondered if anyone else is using 
this?Is this available as a ready to use?   Or do you have to make it up?   
What vendors are you using and could you provide a procedure about how you use 
itlength of time in solution, etc.

Thanks in advance for your help.
 
Martha Ward, MT (ASCP) QIHC
Manager

Molecular Diagnostics Lab
Medical Center Boulevard  \  Winston-Salem, NC 27157 p 336.716.2109  \  f 
336.716.5890 mw...@wakehealth.edu  
 
 






___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] RE: Water baths

[Pathology-Histology Sr. Supervisor]

 We have been checking ALL temps and recording every shift. These include 
cryostats, Embedding centers, Wax dispenser, Hot plates, Hot Ovens, Body 
Refrigerator, refrigerators, Water Baths, and room temps and Humidity%.

Muhammad Tahseen MLT (PMF), MT (JIMTEF) Japan
Senior supervisor histopathology
SKMCHRC Lahore
Pakistan


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Abbott, Tanya
Sent: Tuesday, December 02, 2014 8:29 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Water baths

Does anyone take daily temperature checks of their water bath (used for cutting 
only) for CAP?

Tanya G. Abbott RT (CSMLS)
Manager Technologist, Histology/Cytology St. Joseph Medical Center Reading, PA 
19603-0316 ph  610-378-2635 fax 610-898-5871
email: tanyaabb...@catholichealth.net

This email and attachments contain information that may be confidential or 
privileged. If you are not the intended recipient, notify the sender at once 
and delete this message completely from your information system. Further use, 
disclosure, or copying of information contained in this email is not 
authorized, and any such action should not be construed as a waiver of 
privilege or other confidentiality protections.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] EDTA decalcification solution

[Martha Ward-Pathology]

I am asking this question for our Histology Lab.  They are being asked about 
using EDTA as a decal solution for bone and wondered if anyone else is using 
this?Is this available as a ready to use?   Or do you have to make it up?   
What vendors are you using and could you provide a procedure about how you use 
itlength of time in solution, etc.

Thanks in advance for your help.
 
Martha Ward, MT (ASCP) QIHC
Manager

Molecular Diagnostics Lab
Medical Center Boulevard  \  Winston-Salem, NC 27157
p 336.716.2109  \  f 336.716.5890  
mw...@wakehealth.edu  
 
 






___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] RE: Specimen numbering systems

[Pathology-Histology Sr. Supervisor]

I also agree with Dawn. We shall be start from Jan 2015 as this way.

Muhammad Tahseen, MT (JIMTEF) Japan
Senior supervisor Histopathology
SKMCHRC Lahore
Pakistan

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Roy, Ryan
Sent: Tuesday, November 25, 2014 12:42 AM
To: 'hymclab'; 'Rathborne, Toni'; 'Cartun, Richard'; Willis, Donna G.; Histonet 
(histonet@lists.utsouthwestern.edu)
Subject: [Histonet] RE: Specimen numbering systems

I agree with Dawn . Basically use a numeric for the Block and an Alpha for the 
specimen. This way a large resection specimen would receive an A and all blocks 
for said specimen would be labeled as A1,A2,A3...ect 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of hymclab
Sent: Monday, November 24, 2014 2:26 PM
To: 'Rathborne, Toni'; 'Cartun, Richard'; Willis, Donna G.; Histonet 
(histonet@lists.utsouthwestern.edu)
Subject: [EXTERNAL] [Histonet] RE: Specimen numbering systems

We do it the same as Toni and from the CAP Uniform Labeling of slides and 
Blocks presentation I attended at NSH that is what CAP came up with for uniform 
labeling (S14-1234 A1, A2, A3, B1, B2, etc...).



Dawn Schneider, HT(ASCP)
Howard Young Medical Center
240 Maple Ave.
Woodruff, WI 54568
715-356-8174




-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni
Sent: Friday, November 21, 2014 2:18 PM
To: 'Cartun, Richard'; Willis, Donna G.; Histonet 
(histonet@lists.utsouthwestern.edu)
Subject: [Histonet] RE: Specimen numbering systems

We use a numeric for the accession number (S-14-1234), followed by alpha for 
the specimen(A, B, C), and within that have numeric for the number of blocks in 
each specimen (A1, A2, A3). We haven't ever gone to Z in our current LIS, and 
the ability to add unlimited blocks to each specimen makes it easy to deal with.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cartun, Richard
Sent: Friday, November 21, 2014 3:11 PM
To: Willis, Donna G.; Histonet (histonet@lists.utsouthwestern.edu)
Subject: [Histonet] RE: Specimen numbering systems

We are currently using Numeric for the specimen (or part) and Alpha for the 
block.  I don't like it; we frequently have 1Z blocks for large CA 
resections!  I would like to change this going forward.

Richard

Richard W. Cartun, MS, PhD
Director, Histology  Immunopathology
Director, Biospecimen Collection Programs Assistant Director, Anatomic 
Pathology Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 972-1596
(860) 545-2204 Fax

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Willis, Donna G.
Sent: Friday, November 21, 2014 2:03 PM
To: Histonet (histonet@lists.utsouthwestern.edu)
Subject: [Histonet] Specimen numbering systems

Can large facilities of more than 500 beds please let me know how they are 
numbering their Surgical specimens.  Alpha for the Specimens and numeric for 
the Block or Numeric for the Specimen and Alpha for the Block.

Thanks,

Donna Willis, HT/HTL(ASCP)
Anatomic Pathology Manager

Baylor University Medical Center
3500 Gaston Ave|Dallas, Texas  75246
214-820-2465 office|214-725-6184 mobile
BaylorScottandWhite.com


**
This e-mail may contain confidential and/or privileged information. This 
information is intended only for the use of the individual(s) and entity(ies) 
to whom it is addressed. If you are the intended recipient, further disclosures 
are prohibited without proper authorization. If you are not the intended 
recipient (or have received this e-mail in error) please notify the sender 
immediately and destroy this e-mail. Any unauthorized copying, disclosure or 
distribution of the material in this e-mail is strictly forbidden and possibly 
a violation of federal or state law and regulations. Baylor Health Care System, 
its subsidiaries, and affiliates hereby claim all applicable privileges related 
to this information.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

This e-mail message, including any attachments, is for the sole use of the 
intended recipient(s) and may contain confidential and privileged information. 
Any unauthorized review, use, disclosure, or distribution is prohibited. If you 
are not the intended recipient, or an employee or agent responsible for 
delivering the message to the intended recipient, please contact the sender by 
reply e-mail and destroy all copies of the original message, including any 
attachments

[Histonet] RE: Squamous cells staining on HE and IHC

[Pathology-Histology Sr. Supervisor]

When the sections are cut and taken on the slide from water bath, slide must 
only holds on the frosted end of the slides don't touch other part of slide.
Regards
Muhammad Tahseen
MLT (JIMTEF) Japan
Histology Supervisor
SKMCHRC
Lahore
Pakistan

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Amber McKenzie
Sent: Wednesday, October 29, 2014 12:55 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Squamous cells staining on HE and IHC


Does anyone else have problems with what looks like squamous cells staining on 
your HE's and IHC's?  I'm trying to figure out how to eliminate that problem 
in our lab...wear gloves while cutting?  Change out water bath several times 
during shifts? Any suggestions?  Thanks!

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] RE: Working on Saturday

[Pathology-Histology Sr. Supervisor]

Dear Lorraine,
Our Histology departments work on Saturday.
Muhammad Tahseen
MLT (JIMTEF) Japan
Histology Supervisor
SKMCHRC
Lahore
Pakistan

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Smallwood, 
Lorraine
Sent: Thursday, October 23, 2014 10:28 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Working on Saturday

We are a 450 bed hospital.  Due to our size and volume of work, our work week 
is Monday-Saturday. Because of healthcare reform, budget cuts and staffing 
shortage, we are faced with closing the department on Saturday (!!Histotechs 
are happy!!). One tech will continue to come in on Saturday to process any 
breast specimens received.   I am curious to find out what is the norm 
concerning working on Saturdays.  How many other Histology departments work on 
Saturday?  Thanks in advance for your opinions!

Lorraine Smallwood MScEd, PA(ASCP)
BB(ASCP)IHC, MLS(ASCP)HT, MT(AMT)
Pathologist Assistant


Disclaimer:
The HIPAA Final Privacy Rule requires covered entities to safeguard certain 
Protected Health Information (PHI) related to a person's healthcare. 
Information being faxed to you may include PHI after appropriate authorization 
from the patient or under circumstances that do not require patient 
authorization. You, the recipient, are obligated to maintain PHI in a safe and 
secure manner. You may not re-disclose without additional patient consent or as 
required by law. Unauthorized re-disclosure or failure to safeguard PHI could 
subject you to penalties described in federal (HIPAA) and state law. If you the 
reader of this message are not the intended recipient, or the employee or agent 
responsible to deliver it to the intended recipient, please notify us 
immediately and destroy the related message.

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

RE: [Histonet] Paraffin Used

[Pathology-Histology Sr. Supervisor]

We infiltrate and embed with Paraplast Plus Surgipath.
Regards,
Muhammad Tahseen
MLT (JIMTEF) Japan
Histology Supervisor
SKMCHRC
Lahore
Pakistan


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Elizabeth 
Chlipala
Sent: Friday, October 24, 2014 3:53 AM
To: Cooper, Brian; WILLIAM DESALVO; histonet
Subject: RE: [Histonet] Paraffin Used

We do the same here.

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 
Boulder, CO 80308
(303) 682-3949 office
(303) 682-9060 fax
(303) 881-0763 cell
l...@premierlab.com
www.premierlab.com

March 10, 2014 is Histotechnology Professionals Day

Ship to Address:

Premier Laboratory, LLC
1567 Skyway Drive, Unit E
Longmont, CO 80504


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cooper, Brian
Sent: Thursday, October 23, 2014 4:37 PM
To: WILLIAM DESALVO; histonet
Subject: RE: [Histonet] Paraffin Used

Hi Bill, 

We infiltrate with Paraplast, and embed with Paraplast Extra.

Thanks, 

Brian D. Cooper, HT (ASCP)CM | Histology Supervisor Department of Pathology and 
Laboratory Medicine Children's Hospital Los Angeles
4650 Sunset Blvd MS#43- Los Angeles, CA 90027
Ph: 323.361.3357 Pager: 213-209-0184 bcoo...@chla.usc.edu 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of WILLIAM DESALVO
Sent: Thursday, October 23, 2014 11:48 AM
To: histonet
Subject: [Histonet] Paraffin Used

What paraffin do you use in your lab? Do you use different type for processing 
and embedding? Looking to investigate the top used paraffins for a workshop I 
am putting together.

Sent from my iPhone
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



-
CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for 
the sole use of the intended recipient(s) and may contain confidential or 
legally privileged information. Any unauthorized review, use, disclosure or 
distribution is prohibited. If you are not the intended recipient, please 
contact the sender by reply e-mail and destroy all copies of this original 
message.  

-


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] RE: Double Labeling EM Blocks

[Pathology-Histology Sr. Supervisor]

We are labeling all Blocks and slides with two identifier as hospital policy.
Regards
Muhammad Tahseen
MLT (JIMTEF) Japan
Histology Supervisor
SKMCHRC
Lahore
Pakistan


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Neelam Joshi
Sent: Thursday, October 23, 2014 5:42 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Double Labeling EM Blocks

Hello,

Does anyone has a procedure for double labeling EM Blocks with two identifier?

Please let me know as soon as possible.

Thanks you all!

Neelam Joshi,HT
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] FT NIGHT SHIFT DELRAY BEACH FL

[Delray Beach Pathology Kari Simeone]

Hi Histonetters! We are looking for a full time licensed histotech here in our 
very busy Delray Florida Dermatology Lab. This is a permanent full time NIGHT 
SHIFT (40 hours) position with benefits (medical/401k/vacation) and shift 
differential. THIS IS A DRUG FREE WORKPLACE.



***PLEASE NO HEAD HUNTERS/PLACEMENT SERVICES***!!!



Email your resume to lengim...@leavittmgt.commailto:lengim...@leavittmgt.com 
if interested.



*full time position Mon-Fri PM (will discuss hours upon interview/will be AFTER 
5p)

*MUST be licensed as a FL histotechnologist or technician

*MUST have at LEAST 2 years experience (dermatology preferred) Please DO NOT 
respond if no EXPERIENCE!

*VERY proficient in embedding and microtomy

*must be self motivated, reliable and a team player

*knowledge in operating Ventana and Leica equipment desired (not necessary)



Kari M Simeone

561.819.6517 fax

ksime...@leavittmgt.commailto:ksime...@leavittmgt.com

www.advancedderm.comhttp://www.advancedderm.com/



The information contained in this message and any attachments is intended only 
for the use of the individual or entity to which it is addressed, and may 
contain information that is privileged, confidential and exempt from disclosure 
under applicable law. If you have received this message in error, you are 
prohibited from copying, distributing or using the information. Please contact 
the sender immediately by return e-mail and delete the original message.


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] FT NIGHT SHIFT Histotech position in DELRAY BEACH, FL

[Delray Beach Pathology Kari Simeone]

Hi Histonetters! We are looking for a full time licensed histotech here in our 
very busy Delray Florida Dermatology Lab. This is a permanent full time NIGHT 
SHIFT (40 hours) position with benefits (medical/401k/vacation) and shift 
differential. THIS IS A DRUG FREE WORKPLACE.



***PLEASE NO HEAD HUNTERS/PLACEMENT SERVICES***!!!



Email your resume to lengim...@leavittmgt.commailto:lengim...@leavittmgt.com 
if interested.



*full time position Mon-Fri PM (will discuss hours upon interview/will be AFTER 
5p)

*MUST be licensed as a FL histotechnologist or technician

*MUST have at LEAST 2 years experience (dermatology preferred) Please DO NOT 
respond if no EXPERIENCE!

*VERY proficient in embedding and microtomy

*must be self motivated, reliable and a team player

*knowledge in operating Ventana and Leica equipment desired (not necessary)



Kari M Simeone

Histology/Immunohistochemistry Specialist Supervisor

Alternate Laboratory Supervisor

ksime...@leavittmgt.commailto:ksime...@leavittmgt.com





The information contained in this message and any attachments is intended only 
for the use of the individual or entity to which it is addressed, and may 
contain information that is privileged, confidential and exempt from disclosure 
under applicable law. If you have received this message in error, you are 
prohibited from copying, distributing or using the information. Please contact 
the sender immediately by return e-mail and delete the original message.


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] RE: questions

[Martha Ward-Pathology]

Sounds like we are all working at the same place!   LOL   
I hear exactly the same questions.is it something they teach to all 
pathology residents

 
Martha Ward, MT (ASCP) QIHC
Manager

Molecular Diagnostics Lab
Medical Center Boulevard  \  Winston-Salem, NC 27157
p 336.716.2109  \  f 336.716.5890  
mw...@wakehealth.edu  
 
 



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy
Sent: Thursday, August 07, 2014 1:18 PM
To: 'Grantham, Andrea L - (algranth)'; Webb, Dorothy L; 
'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] RE: questions

... and I can still hear myself explaining to them that there could only be 
ONE first!

Yes, DAILY!!! Every time someone wants to change things around so they get 
theirs first, this is our only defense as to why everyone can't get theirs 
first. 8 years of college doesn't seem to help here...

Tim Morken
Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC 
San Francisco Medical Center San Francisco, CA


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Grantham, 
Andrea L - (algranth)
Sent: Thursday, August 07, 2014 10:08 AM
To: Webb, Dorothy L; 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] RE: questions

When I worked in clinical labs the order was first the priority cases 
(biopsies, bone marrows, etc.) then numerically.
I remember once two physicians brought small biopsies and were in a hurry for 
the results, both wanted to be first out and I can still hear myself explaining 
to them that there could only be ONE first!

Andi

From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] on behalf of Webb, Dorothy L 
[dorothy.l.w...@healthpartners.com]
Sent: Thursday, August 07, 2014 9:54 AM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] questions

I have a couple of questions to ask where there is no right or wrong answer, 
just curious as to the process that other labs use.
1.  After processing, how do you determine the order in which to cut and stain 
the blocks..numerical or priority driven?  2. Do you adhere to the 6-72 hours 
of fixation for breasts or make certain all breast tissue is fixed for a 
minimum time of, say, 24 hours but, of course no longer than 72?

I appreciate your responses and thanks for your time!!  I am retiring at the 
end of this year and trying to optimize some processes beforehand:).

Dorothy Webb, HT (ASCP)
Regions Histology Technical Specialist
651-254-2962



  
This e-mail and any files transmitted with it are confidential and are intended 
solely for the use of the individual or entity to whom they are addressed. If 
you are not the intended recipient or the individual responsible for delivering 
the e-mail to the intended recipient, please be advised that you have received 
this e-mail in error and that any use, dissemination, forwarding, printing, or 
copying of this e-mail is strictly prohibited.

If you have received this communication in error, please return it to the 
sender immediately and delete the original message and any copy of it from your 
computer system. If you have any questions concerning this message, please 
contact the sender. Disclaimer R001.0 
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

RE: [Histonet] Validation of IHC

[Pathology-Histology Sr. Supervisor]

Dear Jamal,
Please see 
Principles of Analytic Validation of Immunohistochemistry Assays
Published : Archives of Pathology and Laboratory Medicine
March 19, 2014
cap.org
Later on I will send you template of IHC Validation, which we are using in 
SKMCHRC.
Regards
Muhammad Tahseen
Senior Supervisor Histopathology
SKMCHRC
Lahore, Pakistan


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jamal
Sent: Saturday, July 19, 2014 3:42 PM
To: Histonet edu
Subject: [Histonet] Validation of IHC

Dear colleagues

We purchased Ventana GX from Rosh for IHC

I reviewed the resent CAP check list 2013 for validation of IHC but I got 
confused.

I am using ready to use prediluted antibodies from Ventana and I want to 
validate the antibodies before use according to CAP requirements.

Can I have the validation plane and procedure for IHC antibodies.

 

 

 

Best Regards,

 

 

Jamal M. Al Rowaihi  Anatomic Pathology Supervisor   | Al Borg
Medical Laboratories |  Mobile +966 503629832| 
mailto:j.rowa...@alborglaboratories.com j.rowa...@alborglaboratories.com 

Palestine St, Al Rajhi Building, P.O. Box 52817, Jeddah 21573, KSA|
Phone: +966 12 670 0099   | Fax: +966 12 676 4984 |
http://www.alborglaboratories.com/ www.alborglaboratories.com

 

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] FT DERM POSITION DELRAY BEACH, FLORIDA

[Delray Beach Pathology Kari Simeone]

Hi Histonetters! We are looking for a full time licensed histotech here in our 
very busy Delray Florida Derm Lab. This is a permanent full time position with 
benefits (medical/401k/vacation). DRUG FREE WORKPLACE.

***PLEASE NO HEAD HUNTERS/PLACEMENT SERVICES***!!!
 Email your resume to lengim...@leavittmgt.commailto:lengim...@leavittmgt.com 
if interested.

*full time position Mon-Fri 11a-7:30p (hours NON NEGOTIABLE)
*MUST be licensed as a FL histotechnologist or technician
*MUST have at least 2 years experience (dermatology preferred)
*VERY proficient in embedding and microtomy
*experience in grossing and immunohistochemistry (BOND) a plus
*must be self motivated and a team player
*knowledge in operating Ventana and Leica equipment desired (not necessary)



Kari M Simeone

ksime...@leavittmgt.commailto:ksime...@leavittmgt.com







The information contained in this message and any attachments is intended only 
for the use of the individual or entity to which it is addressed, and may 
contain information that is privileged, confidential and exempt from disclosure 
under applicable law. If you have received this message in error, you are 
prohibited from copying, distributing or using the information. Please contact 
the sender immediately by return e-mail and delete the original message.


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] FT DERM POSITION DELRAY BEACH, FLORIDA

[Delray Beach Pathology Kari Simeone]

Hi Histonetters! We are looking for a full time licensed histotech here in our 
very busy Delray Florida Derm Lab. This is a permanent full time position with 
benefits (medical/401k/vacation).

***PLEASE NO HEAD HUNTERS/PLACEMENT SERVICES***!!!
 Email your resume to lengim...@leavittmgt.commailto:lengim...@leavittmgt.com 
if interested.

*full time position Mon-Fri 11a-7:30p (hours NON NEGOTIABLE)
*MUST be licensed as a FL histotechnologist or technician
*MUST have at least 2 years experience (dermatology preferred)
*VERY proficient in embedding and microtomy
*experience in grossing and immunohistochemistry (BOND) a plus
*must be self motivated and a team player
*knowledge in operating Ventana and Leica equipment desired (not necessary)



Kari M Simeone

ksime...@leavittmgt.commailto:ksime...@leavittmgt.com







The information contained in this message and any attachments is intended only 
for the use of the individual or entity to which it is addressed, and may 
contain information that is privileged, confidential and exempt from disclosure 
under applicable law. If you have received this message in error, you are 
prohibited from copying, distributing or using the information. Please contact 
the sender immediately by return e-mail and delete the original message.


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] FT DERM POSITION DELRAY BEACH, FLORIDA

[Delray Beach Pathology Kari Simeone]

Hi Histonetters! We are looking for a full time licensed histotech here in our 
very busy Delray Florida Derm Lab. This is a permanent full time position with 
benefits (medical/401k/vacation).

***PLEASE NO HEAD HUNTERS/PLACEMENT SERVICES***!!!
 Email your resume to lengim...@leavittmgt.commailto:lengim...@leavittmgt.com 
if interested.

*full time position Mon-Fri 11a-7:30p (hours NON NEGOTIABLE)
*MUST be licensed as a FL histotechnologist or technician
*MUST have at least 2 years experience (dermatology preferred)
*VERY proficient in embedding and microtomy
*experience in grossing and immunohistochemistry (BOND) a plus
*must be self motivated and a team player
*knowledge in operating Ventana and Leica equipment desired (not necessary)



Kari M Simeone

ksime...@leavittmgt.commailto:ksime...@leavittmgt.com





The information contained in this message and any attachments is intended only 
for the use of the individual or entity to which it is addressed, and may 
contain information that is privileged, confidential and exempt from disclosure 
under applicable law. If you have received this message in error, you are 
prohibited from copying, distributing or using the information. Please contact 
the sender immediately by return e-mail and delete the original message.


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] IHC proficiency testing

[Delray Beach Pathology Kari Simeone]

Can anyone share their CLIA proficiency protocols/programs for automated IHC 
staining? Other than the obvious antibody validation, positive and negative 
controls, QA and QC performed, should we enroll in an actual program for our 
IHC? We currently use two Leica Bonds and about 70 antibodies. No Her2 or ER/PR 
or FISH.

Thanks in advance!



K. Simeone

ksime...@leavittmgt.commailto:ksime...@leavittmgt.com





The information contained in this message and any attachments is intended only 
for the use of the individual or entity to which it is addressed, and may 
contain information that is privileged, confidential and exempt from disclosure 
under applicable law. If you have received this message in error, you are 
prohibited from copying, distributing or using the information. Please contact 
the sender immediately by return e-mail and delete the original message.


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] RE: rolling sections

[Martha Ward-Pathology]

We do the same thing on our lab.   It isn't necessary for them to rollwe 
just catch them and fold them up and  put them in the tube.
 
Martha Ward, MT (ASCP) QIHC
Manager

Molecular Diagnostics Lab
Medical Center Boulevard  \  Winston-Salem, NC 27157
p 336.716.2109  \  f 336.716.5890  
mw...@wakehealth.edu  
 
 



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Helen Fedor
Sent: Thursday, June 12, 2014 10:55 AM
To: 'Roberta Horner'; Histonet (histonet@lists.utsouthwestern.edu)
Subject: [Histonet] RE: rolling sections

Hi, I think that it is not necessary to actually get them to roll. We just 
collect all of the sections and put them into the tube. Scrunched, not rolled.

Helen

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Roberta Horner
Sent: Thursday, June 12, 2014 10:42 AM
To: Histonet (histonet@lists.utsouthwestern.edu)
Subject: [Histonet] rolling sections

I have some researchers that want to do PCR.  They want 10 - 10u sections in a 
micro-centrifuge tube.  The only way to get the sections in the tube is for the 
sections to roll.  How do you get sections to roll when you want them to roll?  
I've tried room temperature, on ice, brand new sharp blade, dull blade and I 
can still get some really nice ribbons.  When I want a thick ribbon it will 
roll, darn that Murphy and his laws.
Roberta Horner
Animal Diagnostic Lab
Penn State University
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] positive control indoleamine 2-3 dioxygenase

[Pathology-Histology Sr. Supervisor]

Hi All,
We are going to optimize indoleamine 2-3 dioxygenase (IDO1) expression in 
breast cancer tissue by immunohistochemistry on formalin fixed paraffin 
sections.
I shall be thankful  if any one please let me know about the positive control.
Regards
Muhammad Tahseen
Sr.Supervisor Histopathology
SKMCHRC Lahore
Pakistan
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

RE: [Histonet] Re: Negative Controls

[Martha Ward-Pathology]

So do we.

 
Martha Ward, MT (ASCP) QIHC
Manager

Molecular Diagnostics Lab
Medical Center Boulevard  \  Winston-Salem, NC 27157
p 336.716.2109  \  f 336.716.5890  
mw...@wakehealth.edu  
 
 



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sebree Linda A
Sent: Wednesday, April 30, 2014 8:35 AM
To: 'Campbell, Tasha M.'; Terri Braud; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Re: Negative Controls

Tasha,

We use the negative elements within our patient sample as our negative tissue 
control.

Linda A. Sebree
University of Wisconsin Hospital  Clinics IHC/ISH Laboratory
600 Highland Ave. 
Madison, WI 53792
(608)265-6596
FAX: (608)262-7174 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Campbell, Tasha 
M.
Sent: Wednesday, April 30, 2014 6:48 AM
To: Terri Braud; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Re: Negative Controls

So this has confused me more. So before you would run a negative control for 
each block you were testing and you would use the negative mouse or rabbit 
reagent.  Now you don't have to do that but you still need a negative tissue 
control.  So what exactly does this mean?  Does it mean for every antibody that 
you are running you need to have a negative tissue control for it?  So instead 
of using the negative mouse serum you would run a known negative tissue control 
with the antibody, say CD3 or whatever it is?  So are most people doing a 
control slide with a negative tissue and a positive tissue on it?

 
 
Tasha Campbell, B.S.,HTL(ASCP)
Frederick Gastroenterology Associates
310 W. 9th St.
Frederick, MD 21701
301-695-6800 ext. 144 (w)
304-685-9307 (c)
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Terri Braud
Sent: Tuesday, April 29, 2014 1:30 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Re: Negative Controls

On Message 7 - Negative Controls
While it is true that if you run polymers, you no longer have to run a negative 
reagent control, HOWEVER, you still must have a negative tissue control, which 
to quote CAP: must  show no staining of tissues known to lack the antigen
Any of the following can serve as a negative tissue control:
1. Multi tissue blocks.  These can provide simultaneous positive and negative 
tissue controls and are considered best practice...
The type of negative tissue control used (i.e. separate sections, internal 
controls, or multitissue blocks) must be specified in the laboratory manual.
Thus sayeth CAP, the almighty.  Please see ANP.22570 Our lab has defined our 
negative controls as a piece of Uterus as the negative tissue in a multitissue 
block as a negative tissue control for most of our antibodies, though for a few 
that might be too reactive in uterus, we use a piece of skin.
I hope this helps.

Terri L. Braud, HT(ASCP)
Anatomic Pathology Supervisor
Holy Redeemer Hospital Laboratory
1648 Huntingdon Pike
Meadowbrook, PA 19046
Ph: 215-938-3676
Fax: 215-938-3874

Message: 7
From: Beth Brinegar bbrinegar...@gmail.com
Subject: [Histonet] Negative controls
Hello fellow histonetters,

What is are other labs doing to satisfy the ANP.22570 QC - Antibodies 
Appropriate negative controls are used.



-



CONFIDENTIALITY NOTICE:

This E-Mail is intended only for the use of the individual or entity to which 
it was sent. It may contain information that is privileged and/or confidential, 
and the use or disclosure of such information may also be restricted under 
applicable federal and state law. If you received this communication in error, 
please do not distribute any part of it or retain any copies, and delete the 
original E-Mail.
Please notify the sender of any error by E-Mail.

Thank you for your cooperation.


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] CAP question ANP.22978 - Her2 assay validation

[Martha Ward-Pathology]

Hello all,

I have been reading through the most recent revisions and want to see how 
others are handling this question.   The explanation states that it is for new 
and existing assays and that if your validation does not meet current standards 
that you must supplement and bring it into compliance.  Furthermore if you do 
not have any documentation from the initial validation the assay must be fully 
revalidated and documented.

Our lab has been performing the Herceptest from Dako (FDA approved) since 
before 2008 and participating in the HER2 proficiency testing since it was 
first offered.   We have our statistical results comparing our IHC patient 
results to FISH Her2 results since 2008 and we have always done well on our CAP 
proficiency testing (95%-100%).We do inter-pathologist result comparisons, 
using know CAP slides and have 95% to 100% agreements.   

What I do not have however is the original results of the slides that were 
stained to set up the original assay.   Under these circumstances will we need 
to completely revalidate the assay, using the mandated 20+/20- cases, or can we 
simply do a retroactive formal review and write up of our past performances on 
our proficiency testing challenges?

Thanks in advance for your help with this!

 
Martha Ward, MT (ASCP) QIHC
Manager
 
Molecular Diagnostics Lab
Medical Center Boulevard  \  Winston-Salem, NC 27157


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] company for antibody HSV(cocktail) on paraffin section

[Pathology-Histology Sr. Supervisor]

Hi All
We are looking company for antibody HSV(cocktail) on paraffin section, kindly 
any comments.
Regards
Muhammad Tahseen
Histology supervisor
SKMCHRC
Lahore

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] BioVision Autostanier RsT

[Delray Beach Pathology Kari Simeone]

Hi all! Does anyone have a PAS-F protocol for the old BioVision Autostainer 
they can share? We have one but the pathologist is complaining there isn't 
enough differentiation (too blue). It currently runs for 10 min in Periodic 
Acid  20 minutes in Schiff's. I would appreciate it!



Kari M Simeone

Histology/Immunohistochemistry Specialist Supervisor

Alternate Laboratory Supervisor

Delray Beach Technical Laboratory

ksime...@leavittmgt.commailto:ksime...@leavittmgt.com



The information contained in this message and any attachments is intended only 
for the use of the individual or entity to which it is addressed, and may 
contain information that is privileged, confidential and exempt from disclosure 
under applicable law. If you have received this message in error, you are 
prohibited from copying, distributing or using the information. Please contact 
the sender immediately by return e-mail and delete the original message.


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Recommendations cryostat

[Pathology-Histology Sr. Supervisor]

Hi All,
We are going to buy cryostat so opinion required CM 1950  LEICA verses HM525 NX 
Thermo.
Regards
Muhammad Tahseen
SKMCHRC




___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] white Sakura ice trays

[Delray Beach Pathology Kari Simeone]

Can anyone tell me what vendor I can purchase the white Sakura ice trays for 
blocks that you can freeze (liquid inside) and put blocks on when cutting. Hope 
this run on sentence makes sense! Thanks in advance. :-)



Kari M Simeone

Histology/Immunohistochemistry Specialist Supervisor

Alternate Laboratory Supervisor

Delray Beach Technical Laboratory

ksime...@leavittmgt.commailto:ksime...@leavittmgt.com





The information contained in this message and any attachments is intended only 
for the use of the individual or entity to which it is addressed, and may 
contain information that is privileged, confidential and exempt from disclosure 
under applicable law. If you have received this message in error, you are 
prohibited from copying, distributing or using the information. Please contact 
the sender immediately by return e-mail and delete the original message.


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] RE: Instrument Verification

[Martha Ward-Pathology]

I'm with you.   There really appears to be no value to this particular 
requirement.I would only be concerned with it if I had just purchased it, 
or moved it into our lab from another location.


 
Martha Ward, MT (ASCP) QIHC
Manager

Molecular Diagnostics Lab
Medical Center Boulevard  \  Winston-Salem, NC 27157
p 336.716.2109  \  f 336.716.5890  
mw...@wakehealth.edu  
 
 



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cynthia Robinson
Sent: Thursday, April 03, 2014 3:03 PM
To: Terri Braud; histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: Instrument Verification

I agree with you in that CAP is just looking for things to change and doesn't 
seem to be considering the change and decrease in staffing seen in clinical 
settings. Cryostat validation? Reallycut a slide after you have cleaned and 
pm'd the thing and go on. Good grief...I don't need any more paper and 
documentation on routine processes. As for tissue processors, I have 20 year 
old VIP's that have been running and producing specimens acceptably. I did 
validate them prior to being put in use but we didn't document like we do now. 
And I don't see the need to do it at this stage of use. We did do a very 
extensive validation on the Peloris we put into use last year and will going 
forward on new equipment.  To me the daily QC of stain should provide our 
'validation' of the process and include the processor. I am interested in 
others thoughts as well.

Thanks for allowing me to rant.

Cindi Robinson, HT(ASCP)
Mercy Medical Center-Sioux City
Dunes Medical Laboratories
350 W Anchor Drive
Dakota Dunes SD 57049




-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Terri Braud
Sent: Thursday, April 03, 2014 1:00 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: Instrument Verification

I just received my midcycle CAP and for cryostat validation, we are planning to 
cut and stain a piece of frozen tonsil and have the path sign off on it. For 
the tissue processors, we will run a one minute test program. I hope this will 
fly.  Is it just me, or is CAP insanely out of control with new or modified 
regulations and policies for AP?  

Terri L. Braud, HT(ASCP)
Anatomic Pathology Supervisor
Holy Redeemer Hospital Laboratory
1648 Huntingdon Pike
Meadowbrook, PA 19046
Ph: 215-938-3676
Fax: 215-938-3874

6. Validation of cryostat (Gloria Tharp)

Message: 6
Date: Thu, 3 Apr 2014 09:59:26 -0500
From: Gloria Tharp gth...@pcasoutheast.com Could anyone tell me how you are 
handling the new CAP ANP.23045 question on function and verification of 
equipment regarding a cryostat.
Gloria Tharp, BA, HTL(ASCP)
--

Message: 7
Date: Thu, 3 Apr 2014 15:26:17 +
From: Leann M. Murphy lmurp...@aultman.com How is everyone validating the 
tissue processor for new CAP ANP.23045 question on function and verification of 
equipment?
LeAnn Murphy
Aultman Hospital
Canton, Ohio

-



CONFIDENTIALITY NOTICE:

This E-Mail is intended only for the use of the individual or entity to which 
it was sent. It may contain information that is privileged and/or confidential, 
and the use or disclosure of such information may also be restricted under 
applicable federal and state law. If you received this communication in error, 
please do not distribute any part of it or retain any copies, and delete the 
original E-Mail.
Please notify the sender of any error by E-Mail.

Thank you for your cooperation.


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] fixed fecal smears for CAP parasitology survey 2014 p3

[Pathology-Histology Sr. Supervisor]

Hi All,
We have received two fixed fecal smears from microbiology section ( CAP 
parasitology survey 2014 p3,)for trichrome or iron-Hematoxylin staining 
procedures.
Would I adopted any kind of trichrome or iron Hematoxylin or some specific ?
Thank,
Muhammad Tahseen
SKMCHRC
Lahore

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] PT/PER DIEM Histotech DELRAY BCH, FL

[Delray Beach Pathology Kari Simeone]

Hi Histonetters! We are still interviewing for a part time tech here in our
very busy east Delray FLORIDA Dermatology Lab (upwards of 80k cases/year).
This is a permanent part time position with full time growth potential.
PLEASE NO HEAD HUNTERS/PLACEMENT SERVICES!!!



 Email your resume to
lengim...@leavittmgt.commailto:lengim...@leavittmgt.com if interested.
MUST BE LICENSED
*part time position AND per diem available with FLEXIBLE TIMES (24 hour
facility) *MUST be licensed as a FL histotechnologist or technician *MUST
have at least 2 years experience (dermatology preferred) *very proficient in
embedding and microtomy *experience in grossing and immunohistochemistry
(BOND) a plus *must be self motivated and a team player (UNPROFESSIONALISM
WILL NOT BE TOLERATED) *knowledge in operating Ventana and Leica equipment
desired (not necessary)



Kari M Simeone

Histology/Immunohistochemistry Specialist Supervisor

Alternate Laboratory Supervisor

Delray Beach Technical Laboratory

ADCS Clinics, LLC

Advanced Dermatology and Cosmetic Surgery

Ameriderm

561.819.0857 ext 100

561.819.6517 fax

ksime...@leavittmgt.commailto:ksime...@leavittmgt.com

www.advancedderm.comhttp://www.advancedderm.com/



The information contained in this message and any attachments is intended only 
for the use of the individual or entity to which it is addressed, and may 
contain information that is privileged, confidential and exempt from disclosure 
under applicable law. If you have received this message in error, you are 
prohibited from copying, distributing or using the information. Please contact 
the sender immediately by return e-mail and delete the original message.


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] negative control for FLI-1

[Pathology-Histology Sr. Supervisor]

Dear All,
We are looking  negative control for FLI-1, as positive control  we are using 
(PNET) on paraffin section successfully.
Our pathologist gave us Wilm's tumor and  IDC for Negative control but it not 
work good.
Thanks
Muhammad Tahseen
Sr. Supervisor Histology
SKMCHRC Lahore
Pakistan
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

RE: [Histonet] Choosing a tissue processor

[Pathology-Histology Sr. Supervisor]

Dear Aminul Islam Khan,
Shandon  Citadel 2000 or Sakura is good for this work load. We are using 
Peloris Leica and Leica ASP 300, our workload are 900 to 1200 blocks /day.
Regards,
Muhammad Tahseen
Senior Supervisor Histopathology
SKMCHRC
Lahore Pakistan

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Md. Aminul 
Islam Khan
Sent: Thursday, February 20, 2014 2:33 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Choosing a tissue processor

Hi everyone,

Can you help us in choosing a tissue autoprocessor. At present we doing manual 
tissue processing and our work load is around 90 to 120 blocks per day which 
may increase gradually. These include tiny biopsies (e.g.
gastric, pleural etc) to large specimens.
Now we want to go for automation.
People here are very poor for which we give the service at a low cost. They 
come from rural areas and staying for extradays in the city is very expensive 
for them.
So we want to shorten the processing time as well.
Also the technical hands are very scarce (i.e. if anything goes wrong with the 
autoprocessor).
What would be the best technology for us? Microwave, linear, crousel or vacuum 
processor?

Thanks in advance and regards,

aikhan
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] HIRING PT and per diem DELRAY BEACH FL

[Delray Beach Pathology Kari Simeone]

Hi Histonetters! We are still interviewing for a part time tech here in our 
very busy east Delray FLORIDA Dermatology Lab (upwards of 80k cases/year). This 
is a permanent part time position with full time growth potential. PLEASE NO 
HEAD HUNTERS/PLACEMENT SERVICES!!!



 Email your resume to lengim...@leavittmgt.commailto:lengim...@leavittmgt.com 
if interested. MUST BE LICENSED
*part time position AND per diem available with FLEXIBLE TIMES (24 hour 
facility)
*MUST be licensed as a FL histotechnologist or technician
*MUST have at least 2 years experience (dermatology preferred)
*very proficient in embedding and microtomy
*experience in grossing and immunohistochemistry (BOND) a plus
*must be self motivated and a team player (UNPROFESSIONALISM WILL NOT BE 
TOLERATED)
*knowledge in operating Ventana and Leica equipment desired (not necessary)



Kari M Simeone

Histology/Immunohistochemistry Specialist Supervisor

Alternate Laboratory Supervisor

Delray Beach Technical Laboratory

ADCS Clinics, LLC

Advanced Dermatology and Cosmetic Surgery

Ameriderm

ksime...@leavittmgt.commailto:ksime...@leavittmgt.com

www.advancedderm.comhttp://www.advancedderm.com/



The information contained in this message and any attachments is intended only 
for the use of the individual or entity to which it is addressed, and may 
contain information that is privileged, confidential and exempt from disclosure 
under applicable law. If you have received this message in error, you are 
prohibited from copying, distributing or using the information. Please contact 
the sender immediately by return e-mail and delete the original message.


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

RE: [Histonet] RE: Negative Controls for IHC

[Martha Ward-Pathology]

We discontinued them over a year ago.


 
Martha Ward, MT (ASCP) QIHC
Manager

Molecular Diagnostics Lab
Medical Center Boulevard  \  Winston-Salem, NC 27157
p 336.716.2109  \  f 336.716.5890  
mw...@wakehealth.edu  
 
 



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of joelle weaver
Sent: Wednesday, February 12, 2014 11:04 AM
To: Cheri Miller
Cc: histonet@lists.utsouthwestern.edu; histonet-boun...@lists.utsouthwestern.edu
Subject: RE: [Histonet] RE: Negative Controls for IHC

Cheryl
Yes, I understand. It is highly likely this will be the way things go here 
also. 




Joelle Weaver MAOM, HTL (ASCP) QIHC
 
 From: cmil...@physlab.com
 To: joellewea...@hotmail.com
 CC: histonet-boun...@lists.utsouthwestern.edu; 
 histonet@lists.utsouthwestern.edu
 Date: Wed, 12 Feb 2014 08:04:43 -0600
 Subject: RE: [Histonet] RE: Negative Controls for IHC
 
 I just stopped using the reagent controls on all our IHC due to the drastic 
 cuts in reimbursement. It will be a significant immediate savings for our 
 tiny lab.
 Cheryl A. Miller HT ASCP cm
 Histology Supervisor
 Hygiene Officer
 Physicians Laboratory, P.C.
 4840 F St.
 Omaha , NE. 68117
 402 731 4145 ext. 532
 Cell 402 493 0403
 
 From: histonet-boun...@lists.utsouthwestern.edu 
 [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of joelle weaver 
 [joellewea...@hotmail.com]
 Sent: Tuesday, February 11, 2014 1:54 PM
 To: Sebree Linda A; 'Abbott, Tanya'; histonet@lists.utsouthwestern.edu
 Subject: RE: [Histonet] RE: Negative Controls for IHC
 
 Not too long ago, there was a posting about labs that had discontinued. You 
 technically don't need with a polymer, non-biotin system. I still do them- 
 for now. The pathologist never uses them, internal controls only. But I do, 
 but this may be a good cost cutting idea for the future.
 
 
 
 
 Joelle Weaver MAOM, HTL (ASCP) QIHC
 
  From: lseb...@uwhealth.org
  To: tanyaabb...@catholichealth.net; 
  histonet@lists.utsouthwestern.edu
  Date: Tue, 11 Feb 2014 19:44:37 +
  CC:
  Subject: [Histonet] RE: Negative Controls for IHC
 
  Hi Tanya,
 
  We have made the decision to continue running negative reagent control 
  slides.
 
  Linda A. Sebree
  University of Wisconsin Hospital  Clinics IHC/ISH Laboratory
  600 Highland Ave.
  Madison, WI 53792
  (608)265-6596
  FAX: (608)262-7174
 
  -Original Message-
  From: histonet-boun...@lists.utsouthwestern.edu 
  [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of 
  Abbott, Tanya
  Sent: Tuesday, February 11, 2014 12:54 PM
  To: histonet@lists.utsouthwestern.edu
  Subject: [Histonet] Negative Controls for IHC
 
  I just read in Advance Dec 2013 that there is a possibility of laboratories 
  utilizing fewer QC controls to cut costs? Has anyone stopped running 
  negative controls for IHC?
 
  Tanya G. Abbott RT (CSMLS)
  Manager Technologist, Histology/Cytology St. Joseph Medical Center 
  Reading, PA 19603-0316 ph  610-378-2635 fax 610-898-5871
  email: tanyaabb...@catholichealth.net
 
  This electronic mail and any attached documents are intended solely for the 
  named addressee(s) and contain confidential information. If you are not an 
  addressee, or responsible for delivering this email to an addressee, you 
  have received this email in error and are notified that reading, copying, 
  or disclosing this email is prohibited. If you received this email in 
  error, immediately reply to the sender and delete the message completely 
  from your computer system.
  ___
  Histonet mailing list
  Histonet@lists.utsouthwestern.edu
  http://lists.utsouthwestern.edu/mailman/listinfo/histonet
 
  ___
  Histonet mailing list
  Histonet@lists.utsouthwestern.edu
  http://lists.utsouthwestern.edu/mailman/listinfo/histonet
   
 ___
 Histonet mailing list
 Histonet@lists.utsouthwestern.edu
 http://lists.utsouthwestern.edu/mailman/listinfo/histonet
 
 PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message.  If 
 you are not the addressee intended / indicated or agent responsible for 
 delivering it to the addressee, you are hereby notified that you are in 
 possession of confidential and privileged information.  Any dissemination, 
 distribution, or copying of this e-mail is strictly prohibited.  If you have 
 received this message in error, please notify the sender immediately and 
 delete this email from your system.
 
  
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu

RE: [Histonet] Turn Around Time

[Martha Ward-Pathology]

Rene,

No not the quality of the slides at all.  Actually my lab does not do the 
processing and initial HE, which is done in our main histology lab.   My 
concern is that the push for quicker TATs sometimes leads to inadequate 
fixation, etc., which affects our lab.I'm just saying that fast does not 
always with better, but there is no reason not to stream line our processes and 
try to provide more timely results.

Of course any case requiring our lab services (IHC, ISH, PCR) is going to have 
a longer TAT.   We generally have a 24 hour TAT on our IHC and ISH and of 
course longer on our PCR testing as those are batched.

More of an editorial comment that anything else

Martha

From: Rene J Buesa [mailto:rjbu...@yahoo.com]
Sent: Monday, February 03, 2014 4:38 PM
To: Martha Ward-Pathology; Dawn Bugge; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Turn Around Time

Martha:
So, do you mean that you are not satisfied with the quality of your slides?
If you are, I just do not understand your concerns about a 24 h TAT!
The fundamental issue is to develop adequate protocols assuring quality as well 
as a convenient (24 h) TAT.
Just my 3 cents (after inflation!)
René J.

From: Martha Ward-Pathology mw...@wakehealth.edumailto:mw...@wakehealth.edu
To: Dawn Bugge drbu...@gmail.commailto:drbu...@gmail.com; 
histonet@lists.utsouthwestern.edumailto:histonet@lists.utsouthwestern.edu 
histonet@lists.utsouthwestern.edumailto:histonet@lists.utsouthwestern.edu
Sent: Monday, February 3, 2014 2:05 PM
Subject: RE: [Histonet] Turn Around Time

While I recognize the need for a quick result in some cases, I also subscribe 
to the theory that speed kills.  I'm not sure that these quick TATs are 
always medically necessary, but rather more of a convenience.However that 
is another discussion altogether.  That said, our institution shoots for the 
80% in 24 hours as well.



Martha Ward, MT (ASCP) QIHC
Manager

Molecular Diagnostics Lab
Medical Center Boulevard  \  Winston-Salem, NC 27157
p 336.716.2109  \  f 336.716.5890
mw...@wakehealth.edumailto:mw...@wakehealth.edu





-Original Message-
From: 
histonet-boun...@lists.utsouthwestern.edumailto:histonet-boun...@lists.utsouthwestern.edu
 
[mailto:histonet-boun...@lists.utsouthwestern.edumailto:histonet-boun...@lists.utsouthwestern.edu]
 On Behalf Of Dawn Bugge
Sent: Monday, February 03, 2014 12:43 PM
To: histonet@lists.utsouthwestern.edumailto:histonet@lists.utsouthwestern.edu
Subject: [Histonet] Turn Around Time

Hello Histonet

I am just curious what the standard for Turn Around Time is for most labs.
I think a two day turn around time from the time the biopsy gets to the lab to 
the time the pathologist signs out a case is pretty fast.

Thanks for your input.
--
Dawn R Bugge
Seattle Histology
___
Histonet mailing list
Histonet@lists.utsouthwestern.edumailto:Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


___
Histonet mailing list
Histonet@lists.utsouthwestern.edumailto:Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

RE: [Histonet] Turn Around Time

[Martha Ward-Pathology]

While I recognize the need for a quick result in some cases, I also subscribe 
to the theory that speed kills.   I'm not sure that these quick TATs are 
always medically necessary, but rather more of a convenience.However that 
is another discussion altogether.  That said, our institution shoots for the 
80% in 24 hours as well.


 
Martha Ward, MT (ASCP) QIHC
Manager

Molecular Diagnostics Lab
Medical Center Boulevard  \  Winston-Salem, NC 27157
p 336.716.2109  \  f 336.716.5890  
mw...@wakehealth.edu  
 
 



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Dawn Bugge
Sent: Monday, February 03, 2014 12:43 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Turn Around Time

Hello Histonet

I am just curious what the standard for Turn Around Time is for most labs.
I think a two day turn around time from the time the biopsy gets to the lab to 
the time the pathologist signs out a case is pretty fast.

Thanks for your input.
--
Dawn R Bugge
Seattle Histology
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] RE: HER2/NEU

[Martha Ward-Pathology]

That is an interesting observation, as we have seen something of the same thing 
and we use the Dako Herceptest.I would be interested in hearing others 
responses as well.   Thank you for asking the  question.


 
Martha Ward, MT (ASCP) QIHC
Manager

Molecular Diagnostics Lab
Medical Center Boulevard  \  Winston-Salem, NC 27157
p 336.716.2109  \  f 336.716.5890  
mw...@wakehealth.edu  
 
 



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Knutson, Deanne
Sent: Wednesday, January 15, 2014 11:46 AM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] HER2/NEU

I just wanted to see what others in the histology world might be experiencing.

We have seen a marked increase in the percentage of HER2 cases resulting as 
Indeterminate, when we compare our last year patient predictive marker results 
to this year's results.
The clone we are using is CB11 from Cell Marque.

We did a HER2 study last year where we sent out our cases for a second opinion, 
and the study showed others interpreted our Indeterminates as that also.
Has anyone else also noted this increase in their annual comparison, and what 
clone are you using?

It is so nice to be able to reach out to our peers with these types of 
questions.
Thank you for responding back!

Deanne Knutson
Anatomic Pathology Supervisor
St. Alexius Medical Center
701-530-6730
dknut...@primecare.orgmailto:dknut...@primecare.org




This email may include confidential and privileged information. If this is not 
intended for your use, please destroy immediately and contact the sender of the 
message.

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] IHC QC

[Delray Beach Pathology Kari Simeone]

Hello and happy new year! I was wondering what other labs (NOT CAP certified) 
are doing for their daily QC sign off on each antibody run? I have about 5 docs 
and each order anywhere from 2-50 IHC stains daily. Currently I use the Leica 
Bond and I am pulling and printing case logs (brief slide history) from each 
patient for the docs to sign off. It is A LOT of paperwork. Was hoping to 
reduce this and streamline it. The Bond's specific printing capabilities are 
pretty limited. Do you just have a daily QC list they sign? Would you mind 
sharing that with me? Any suggestions? I really appreciate the input!
Thanks again.

Kari M Simeone
Histology/Immunohistochemistry Specialist Supervisor
Alternate Laboratory Supervisor
ksime...@leavittmgt.com


The information contained in this message and any attachments is intended only 
for the use of the individual or entity to which it is addressed, and may 
contain information that is privileged, confidential and exempt from disclosure 
under applicable law. If you have received this message in error, you are 
prohibited from copying, distributing or using the information. Please contact 
the sender immediately by return e-mail and delete the original message.


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Vendor suggestions for IgG subclass antibodies

[Martha Ward-Pathology]

I am looking for a vendor for FITC labeled IgG1, IgG2 IgG3 and IgG4 antibodies 
for use in our immunofluorescence panels when staining renal biopsies. We 
get most of our FITC antibodies for our panel from Dako but I didn't see these 
others.   Any suggestions are appreciated.   Thanks in advance for your help!


 
Martha Ward, MT (ASCP) QIHC
Manager

Molecular Diagnostics Lab
Medical Center Boulevard  \  Winston-Salem, NC 27157
p 336.716.2109  \  f 336.716.5890  
mw...@wakehealth.edu  
 
 






___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet