Re: [Histonet] Who sells that block deparaffinizer dohickey that everyone has in their lab.

2023-03-02 Thread Porter, Amy via Histonet 
Thermofisher

Sent from my iPhone

> On Mar 1, 2023, at 10:47 PM, Michelle Bell via Histonet 
>  wrote:
> 
> I don’t know the brand, but they are called paratrimmers.  Multiple vendors 
> carry they such as Newcomer or Mercedes.  My techs loved them!
> 
> From: Jay Lundgren via Histonet 
> Sent: Wednesday, March 1, 2023 6:04:37 PM
> To: histonet 
> Subject: [Histonet] Who sells that block deparaffinizer dohickey that 
> everyone has in their lab.
> 
> Who sells that block deparaffinizer doohickey that everyone has in their
> lab.  What brand is it?  Leica?  I don't have one in front of me right now.
> 
>  Thanks,
> 
>  Jay A. Lundgren, M.S., HTL (ASCP)
> 
> 
> 
> Histotechs are the foot soldiers in the battle against Cancer.
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Re: [Histonet] Active Caspase-3 IHC-P staining

2022-06-23 Thread Porter, Amy via Histonet 
What species are you staining??

Amy S. Porter, HT (ASCP) - Lab Supervisor
Michigan State University
Departments of Physiology & Pathology
Investigative HistoPathology Lab
567 Wilson Road - Rm 2133
East Lansing, MI  48824
Ph:  517-884-5026
(she/her/hers)
https://sites.google.com/msu.edu/ihpl/home
"Life is like riding a bicycle. To keep your balance, you must keep moving." - 
Albert Einstein


-Original Message-
From: Charles Riley via Histonet  
Sent: Thursday, June 23, 2022 1:19 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Active Caspase-3 IHC-P staining

I am currently running a research project and the researcher wants me to use 
Caspase-3 for apoptosis staining. I have never worked with this antibody before 
and want to know if anyone has any suggestions on what to use for a positive 
tissue control and a negative tissue control?


I purchased the antibody from Novus Biologicals and they recommend a Jurkat 
cell lysate for positive control but I am not familiar with this as well.

Any guidance would be greatly appreciated 
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[Histonet] Question for Research on Bone

2021-10-28 Thread Porter, Amy via Histonet 
So I am working with Canine femurs and tibias that have been dissected as 
medial and lateral - they have been decaled in formic acid - rinsed in running 
tap well - processed on a routine 1 hour processing program . cutting very 
well!!

My issue is that the articular cartilage on the bone surface keep flipping up 
and over onto itself - I am using a 37C slide warmer overnight to allow them to 
hopefully flatten out more than on the water bath and then drying in the 56C 
oven for about 1 hour - then staining on automated Leica stainer - they are 
intact at 37. look like the might be curling at 56C??

Anyone have any suggestions???

TIA - Amy

Amy S. Porter, HT (ASCP) - Lab Supervisor
Michigan State University
Investigative HistoPathology Lab
567 Wilson Road - Rm 2133
East Lansing, MI  48824
Ph:  517-884-5026
(she/her/hers)
https://sites.google.com/msu.edu/ihpl/home
"Life is like riding a bicycle. To keep your balance, you must keep moving." - 
Albert Einstein



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[Histonet] Research IMF help

2020-09-01 Thread Porter, Amy via Histonet 
To all - I am working with NBF fixed paraffin embedded tissue and need to do 
IMF staining potentially using two different pH retrievals, that are all the 
same primary host species - I know that if I am doing bright field, I would 
retrieve, stain for primary 1 with DAB first and the sequentially retrieve and 
stain for primary 2 with a different chromogen.. any suggestions when 
working with fluorescence?  Does the first reaction get removed from the 
retrieval for the second epitope; I am in new territory and could use some 
recommendations. TIA.  Amy

Amy S. Porter, HT (ASCP) - Lab Supervisor
Michigan State University
Investigative HistoPathology Lab
567 Wilson Road - Rm 2133
East Lansing, MI  48824
Ph:  517-884-5026
"Life is like riding a bicycle. To keep your balance, you must keep moving." - 
Albert Einstein

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Re: [Histonet] Fixed frozen non-paraffin mouse brain

2020-07-04 Thread Porter, Amy via Histonet 
You need to add sucrose to your PFA we use 4%PFA+4%Sucrose to fix and then 
cryoprotect in 30% sucrose which all need to be prepared in Phosphate buffer 
solution NO saline  if you message me directly I would be happy to share 
our SOP this was a very hard thing to learn not a lot in technical 
references about this process.

Sent from my iPhone

> On Jul 4, 2020, at 9:09 PM, Roy, Edward J via Histonet 
>  wrote:
> 
> As a research lab, we sometimes would like to use paraformaldehyde-fixed but 
> non-paraffin embedded tissues; paraffin embedding alters antigens and 
> necessitates antigen retrieval, but simple fixation does not. We have done 
> the traditional 30% sucrose before OCT and freezing, with cryostat 
> sectioning, but results are inconsistent, sometimes producing Swiss-cheese 
> brains. Does anybody have an alternative to 30% sucrose that is more 
> reliable?  I didn’t see anything in the Archives after a search for “30% 
> sucrose”.
> Thanks very much,
> Ed Roy
> 
> 
> Edward J. Roy, PhD
> Professor Emeritus
> Department of Molecular and Integrative Physiology
> University of Illinois at Urbana-Champaign
> Urbana, IL 61801
> 217 333-3375
> 
> 
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Re: [Histonet] IHC DAB

2020-05-01 Thread Porter, Amy via Histonet 
Hydrogen Peroxide gone bad / or not being added???


From: Kristy Castillo via Histonet 
Sent: Friday, May 1, 2020 11:00 AM
To: histonet@lists.utsouthwestern.edu 
Subject: [Histonet] IHC DAB

Hi Histonetters,

We are starting our IHC (fun times), we are having trouble with the DAB
lighting up.  Processed for 5 thru 10 minutes and still nothing.  Our
permanent red is working just fine.  Any ideas.

Thank you!

Kristy
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Re: [Histonet] Routine H frozen section protocol

2020-04-24 Thread Porter, Amy via Histonet 
Fix in 10% NBF - 10 minutes
Rinse in running tap water - 5 minute
Rinse in distilled water
Gill 2 Hematoxylin - 1 1/2 minutes
1% Glacial Acetic water - 3 dips
Running water - 2 minutes
(we do not need bluing agent - our water takes care of it)
95% Ethanol -2 minutes
1% Eosin/Phloxine - 2 minutes
95% Ethanol - 2 minutes
Remaining dehydration in absolute ethanol & xylene - 2 mintues rest of the way 
down




From: Raible, John C. via Histonet 
Sent: Friday, April 24, 2020 8:10 AM
To: histonet@lists.utsouthwestern.edu 
Subject: [Histonet] Routine H frozen section protocol

Hello Histonet,

My name is John Raible, and I work at the VA medical center in Minneapolis. I 
am trying to find routine H frozen section staining protocols for an 
automated linear stainer. My lab recently got one, and I was asked to research 
staining protocols Any help you could provide would be greatly appreciated.

Thank you all. Stay safe and healthy!

John
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Re: [Histonet] FW: Microtome at home

2020-04-16 Thread Porter, Amy via Histonet 
Make sure of insurance coverage and safety for the employee and that they are 
covered in case of injury - are they still clocking in and out in some 
fashion. just thinking in a bigger box.


From: Steven Crochiere via Histonet 
Sent: Thursday, April 16, 2020 6:36 AM
To: histonet@lists.utsouthwestern.edu 
Subject: [Histonet] FW: Microtome at home

Jaime,

I don't see a problem with a research setting. If it was patient care, CLIA 
would need to inspect the set up in the person home. The same goes for our 
pathologists who read slide at home.

Steve

-Original Message-
From: raestask via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Wednesday, April 15, 2020 7:51 PM
To: Jamie Watson ; Histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Microtome at home

I wouldn't think there would be any problem.Rae Staskiewicz HT(ASCP)Sent from 
my Verizon, Samsung Galaxy smartphone
 Original message From: Jamie Watson via Histonet 
 Date: 4/15/20  6:44 PM  (GMT-06:00) To: 
Histonet@lists.utsouthwestern.edu Subject: [Histonet] Microtome at home Hello 
all,Our pathologist has come up with the idea of sending a microtome and 
waterbath home to someone that cannot come to work due to COVID 19.  We are a 
research lab and work with mouse and rat tissue.  Does anyone know of any 
issues with doing this?  I have never heard of anyone cutting slides at home 
other than someone with a private business.Thank 
you.Jamie___Histonet mailing 
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Re: [Histonet] IDH1 Antibody

2018-12-29 Thread Porter, Amy via Histonet 
Excellent!!!

Sent from my iPhone

> On Dec 29, 2018, at 12:04 PM, Oler, April via Histonet 
>  wrote:
> 
> Hi Richard,
> 
> 
> IDH1 is now distributed by ARP.
> 
> https://urldefense.proofpoint.com/v2/url?u=https-3A__www.arp1.com_products_antibodies_monoclonal-2Dantibodies.html_anti-2Didh1-2Dr132h-2Dmonoclonal-2Dantibody-2Dready-2Dto-2Duse.html=DwICAg=nE__W8dFE-shTxStwXtp0A=CqfpxVox3wg0MOYeDRPiDg=pXWGlHMCxF7-l4zy-xdnOLPBuliYlPUuEjbJjtYimmE=fpQH394Vool18mtG0_M-JUYgsVLvwt-00wCEZgaIIOw=
> 
> 
> April Oler
> 
> Lead Tech IHC/DIF/Muscles
> 
> Michigan Medicine
> 
> University of Michigan
> 
> 
> From: Cartun, Richard via Histonet 
> Sent: Friday, December 28, 2018 6:08:29 PM
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] IDH1 Antibody
> 
> External Email - Use Caution
> 
> If you are doing IHC testing for IDH1 here in the United States, do you get 
> the antibody (clone HO9) directly from Dianova in Germany or is there a US 
> distributor?  I did try HistoBiotech, but they no longer carry Dianova 
> antibodies.  Thank you.
> 
> Richard
> 
> Richard W. Cartun, MS, PhD
> Director, Histology & The Martin M. Berman, MD Immunopathology/Morphologic 
> Proteomics Laboratory
> Director, Biospecimen Collection Programs
> Assistant Director, Anatomic Pathology
> Hartford Hospital
> 80 Seymour Street
> Hartford, CT  06102
> (860) 972-1596 (Office)
> (860) 545-2204 (Fax)
> richard.car...@hhchealth.org
> 
> 
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