Re: [Histonet] [EXTERNAL Sender] histologists assisting with transplants

2020-02-13 Thread Rena Fail via Histonet
in the donor liver. Too much fat and it might not be considered for a transplant. Maybe it is just a matter of terminology. After all the pathologist and surgeon called her in at 3am and may have thanked her for her assistance. In 30 years I may have done 3 rapid ORO's on donor livers Rena Fail

Re: [Histonet] On the lighter side...

2014-08-08 Thread Rena Fail
37 years On Friday, August 8, 2014, Jim Burchette jburc...@gmail.com wrote: 40 blessed years that I would not trade for anything. I have met so many wonderful people (well, maybe a few not so wonderful...) and have learned so much. The good Lord has blessed me with a talent that I feel has

Re: [Histonet] Peggy Wenk's passing

2014-07-28 Thread Rena Fail
So sorry to hear of Peggy passing may she have peace. At last On Monday, July 28, 2014, Lee Peggy Wenk lpw...@sbcglobal.net wrote: It is my sad duty to tell you that Peggy Wenk passed away peacefully Saturday morning. Her husband and companion of 37 years and her sister (from Bend,OR) were

Re: [Histonet] Liver bxs

2014-06-19 Thread Rena Fail
Your description is consistent with the tissue having dried out. So observing their procedure may be helpful. Is it placed on gauze then placed in formalin ? That would dry it quickly. are they tending to the pt then placing the specimen in fprmalin, Rena Fail On Thu, Jun 19, 2014 at 9:03 AM

Re: [Histonet] manual tissue processing protocol

2014-02-27 Thread Rena Fail
and more importantly, the thickness of the tissue. You can check the tissue before infiltrating with paraffin.to http://paraffin.to see if it has cleared, If it appears transparent (like a stained glass window.) proceed with paraffin infiltration. * *Rena Fail* On Wed, Feb 26, 2014 at 2:02 PM, Tyrone

Re: [Histonet] (no subject)

2013-08-07 Thread Rena Fail
around my own hand. Though there are some people I worked with that I miss (I've retired) and some aspects of the job I miss, I miss my forceps most of all, Rena Fail On Wed, Aug 7, 2013 at 9:04 AM, Sanjeet Dhirubhai asanj...@yahoo.comwrote: Hi, I am trying lean up at the embedding system. We

Re: [Histonet] Histonet mailing list submission: Jones' Methenamine Silver Stain

2013-07-15 Thread Rena Fail
of the glomerlus is golden brown to black. the temptation is to stop when the bm is defined. Make sure the bowmans capsule is black and the bm in the glom. is dark golden brown to black. BM in thhte skin stains rapidly much faster than thhat in thhe glom Rena fail On Mon, Jul 15, 2013 at 5:13 PM, Anna

Re: [Histonet] Help! Liver mistakenly processed in paraffin (had to be in OCT instead)!

2012-11-13 Thread Rena Fail
Oil Red O is a stain for fat, Alcohol dissolves fat Rehydrating won't help. You can't replace the fat. Rena Fail On Tue, Nov 13, 2012 at 11:52 AM, z o n k e d zon...@gmail.com wrote: Hello Histonetters, First time writer, long time reader. I'm a newbie tech in academia and I was given

Re: [Histonet] Re: special stains for demonstrating intra cytoplasmic DNA viral inclusion bodies

2012-10-13 Thread Rena Fail
Try Lendrum'sstain for inclusion bodies. Rena Fail On Sat, Oct 13, 2012 at 2:48 PM, anjan kumar drvet_an...@hotmail.comwrote: Would like to know special stains for demonstrating intra cytoplasmic viral inclusion bodies tried with shorrs stain giving lot of red color Anybody can suggest

Re: [Histonet] (no subject)

2012-07-31 Thread Rena Fail
Can't beat phone books. Cheap, torn in half they are wider than a kimwipe, you're recycling, and providing therapy in the form of stress relief obtained tearing up phone books. Rena On Tue, Jul 31, 2012 at 2:23 PM, Paula Pierce cont...@excaliburpathology.com wrote: Greetings, does anyone

Re: [Histonet] Counter stain for PAS for fungus (Steve McClain)

2012-07-26 Thread Rena Fail
in all but the structures that have the greatest concentration of carbohydrate Using chromic acid makes it much easier to detect fungus Histologic Dec.2005 XXXVIII(2):35 Rena Fail the first pathologist I worked for had AB-Pas stains done every day and you are right they are wickedly good. On Thu

[Histonet] Tennessee BB

2012-07-24 Thread Rena Fail
Okay 30 years is too long in histology! I should have retired sooner than I did,. I saw this subject heading as a new stain for bacteria instead of an ad for a bed and breakfast in Tennessee. Rena Fail ___ Histonet mailing list Histonet

Re: [Histonet] counter stain for PAS for fungus

2012-07-23 Thread Rena Fail
ml distilled water Rena Fail On Mon, Jul 23, 2012 at 12:11 PM, Michele Carr michelecar...@yahoo.comwrote: Hi everyone I am having a problem with the fast green counterstain for the PAS for fungus. The fast green stain is simply not staining anymore no matter how long I leave

[Histonet] Jim Burchette

2012-06-22 Thread Rena Fail
No muss, no fuss just a generous heart always ready and willing to share. You made life easier in the lab because of your generosity. Thank you. Congratulations on your retirement! Rena Fail HRT (Happily retired Histotech) ___ Histonet mailing list

Re: [Histonet] Elastic Stain

2012-05-24 Thread Rena Fail
Have you tried Resorcin fucshin HE? 1 g RF 100 ml 70% ethanol 1 ml HCL Stain in RF solution 15-30 minutes , according to depth desired wash off excess stain in 95% alcohol Wash in tap water 3-5 minutes Follow with routine HE Rena Fail On Thu, May 24, 2012 at 11:37 AM, Vickroy, Jim

Re: [Histonet] PROTOCOL FOR COLOR CODING BIOPSY CASSETTES

2012-03-23 Thread Rena Fail
It's pretty much up to you,. your boss, the pathologists what colors you use. Red for rushes for example. The colors help identify a group of specimens quickly, then they can be sorted by number. This is especially useful if your SOP is to cut groups of specimens in a specific order. Rena Fail

Re: [Histonet] Xylene and Preggers

2012-01-25 Thread Rena Fail
to protect the employee as well as the employer, Rena Fail On Mon, Jan 23, 2012 at 12:26 PM, Sarah Dysart sdys...@mirnarx.com wrote: Hey all, so 2 things... A. Does anyone have anything saying that .75% (yes less than 1) is an acceptable exposure limit for a pregnant person and B

Re: [Histonet] RE: Amazing

2012-01-05 Thread Rena Fail
0.5% HCL acid ih 70 % ethanol Rena fail On Thu, Jan 5, 2012 at 11:25 AM, Burton, Lynn lynn.bur...@illinois.govwrote: I still use that. It is 70% with 5mL of hydrochloric acid if making 1L. Lynn Burton Lab Assoc I Animal Disease Lab Galesburg, Il 309-344-2451

Re: [Histonet] Cutting speed

2011-12-31 Thread Rena Fail
sections without swollen cells your speed will increase. You will have been working a month by the time the tech goes on vacation and will have the workflow down by then. Don't worry so much. no one will expect you to be as fast as a tech with years of experience. Good luck rena Fail On Sat, Dec 31

Re: [Histonet] vanGieson-Trichrome staining protocol

2010-07-19 Thread Rena Fail
it completely clear then you will probably have added too much ammonia. Rena Fail - Original Message From: Doughty, Adele adoug...@lrri.org To: histonet@lists.utsouthwestern.edu Sent: Mon, July 19, 2010 10:29:19 AM Subject: [Histonet] vanGieson-Trichrome staining protocol Is there anyone

Re: [Histonet] (no subject)

2010-06-16 Thread Rena Fail
, changes 100% ethanol, 3 changes paraffin. for rush processing it was 20 minutes in each. THe bone marrows were cut at 3 microns. i didn't have a problem with tissue washing off.  Rena Fail - Original Message From: Johnson, Nacaela nacaela.john...@usoncology.com To: histonet

Re: [Histonet] possible AFB contaminant

2010-05-20 Thread Rena Fail
for hand staining Rena Fail - Original Message From: debra.or...@uchospitals.edu debra.or...@uchospitals.edu To: histonet@lists.utsouthwestern.edu Sent: Thu, May 20, 2010 3:41:09 PM Subject: [Histonet] possible AFB contaminant Please help, we have had an increase in demands for repeats on our

Re: [Histonet] Freezing spray artifact

2010-05-19 Thread Rena Fail
along these lines. The cracks can be seen microscopically amd interfere with the architecture of the tissue. Rena Fail  - Original Message From: Martin, Erin erin.mar...@ucsf.edu To: histonet histonet@lists.utsouthwestern.edu Sent: Wed, May 19, 2010 12:09:22 PM Subject: [Histonet

Re: [Histonet] alcian blue van gieson stain

2010-03-12 Thread Rena Fail
Alcian Blue first - Original Message From: Perry, Margaret margaret.pe...@sdstate.edu To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Sent: Fri, March 12, 2010 9:30:40 AM Subject: [Histonet] alcian blue van gieson stain We want to try the double stain alcian

[Histonet] Fw: Histo story

2010-03-11 Thread Rena Fail
- Forwarded Message From: Rena Fail renaf...@bellsouth.net To: nmhi...@comcast.net Sent: Thu, March 11, 2010 1:48:12 PM Subject: Histo story A single parent at 29 with 4 children, unpredictable child support, and only a high school education, I could only get minimum wage jobs. I

Re: [Histonet] restaining IHC slides

2009-12-16 Thread Rena Fail
you can use the same slides , repeating all the steps except  the antigen retrieval ( if it is the same ) Ideally you should use new slides, but on tiny bxs, outside slides, or very limited area of interest, that is not aways possible. Rena Fail  - Original Message From: Thomas Pier

Re: [Histonet] rhodanin stain fading

2009-11-20 Thread Rena Fail
Alcohol will cause the stain to fade, even the small amt. that is carried over to the xylene over the course of  the day. Make sure your xylene is fresh and coverslip immediately after staining Rena Fail - Original Message From: Gudrun Lang gu.l...@gmx.at To: histonet