[Histonet] Exciting position at the University of Wisconsin~Madison

Hello all, The School of Veterinary Medicine at the University of Wisconsin, Madison has a full-time position for a Necropsy Technician. The position is responsible for assisting the veterinary pathologists in the biopsy and autopsy service - much like a human hospital but with a much wider

[Histonet] Two University of Wisconsin Positions

Hello, We have two positions open in anatomic pathology at the School of Veterinary Medicine at the University of Wisconsin, Madison, the links are below... https://jobs.hr.wisc.edu/en-us/job/509270/histology-technology-supervisor (application deadline June 10th)

[Histonet] Cassette Labels

Hello,r Has anyone has used printed labels successfully on cassettes that can withstand xylene/paraffin processing and long term storage? (I am NOT looking for a cassette printer...) Thanks! Sandy Sandra J. Cheasty, HT (ASCP) Histology & Necropsy Supervisor UW-Madison, School of

[Histonet] Block Holder for Leica Rm2255

Hello all, The handle snapped off our RM2255 microtome block holder, and the replacement we were sent does not open the clamp far enough. (We are currently managing by using an allen wrench in the drilled out hole...) Does anyone have any suggestions? Cheers, Sandy Sandra J.

[Histonet] New Tissue Processor?

Hello all, Our lab is looking to replace our 10 year old VIP6. We process anywhere from 50 to 200 cassettes daily. Any recommendations are appreciated. Thank you, Sandra Sandra J. Cheasty, HT (ASCP) Histology & Necropsy Supervisor UW-Madison, School of Veterinary Medicine

[Histonet] Autopsy Question

Hello all, Actually it's a necropsy question for those of you who work in veterinary medicine. We perform necropsies on a very large variety of vertebrates that require removal of the spinal cord. (From hedgehogs to bovines.) What equipment are you using to remove spinal cords

[Histonet] Deep Water Bath with Lighting

Ho-ho-ho all, I'm looking for a deep water bath, (3" minimum), that has an interior light source. Most of them seem to be only 2" tall, or have a slanted front control panel that none of us in the lab want. Does anyone know of a brand of flotation water baths that are 3" deep

[Histonet] 10% NBF Substitutes

Hello, Can anyone comment on the current formalin substitutes Excel PLUS and/or FineFIX? -Cost -Benefits -Drawbacks -Performance in tissue processors Thanks! Sandra J. Cheasty, HT (ASCP) Histology & Necropsy Supervisor UW-Madison, School of Veterinary Medicine

[Histonet] Jeepers Creepers, Coverslipping Peepers!

Hello all, Does anyone have a source for oversized coverglass? (35x50, 35x60, 45x50, 45x60) We section a lot of globes from the ophthalmology lab, some of which are from horses, cows, etc. that are put on the larger slides. Fisher used to carry their own brand, but it's

[Histonet] Galileo Microtome

Hello all, Has anyone had experience using the Galileo Series 2 microtome, made by Diapath? Thanks! Sandy Sandra J. Cheasty, HT (ASCP) Histology & Necropsy Supervisor UW-Madison, School of Veterinary Medicine ___ Histonet mailing list

[Histonet] Motorized Microtome Opinions

Hello all, Our only motorized Microm microtome is slowly dying, and I'm looking for a replacement that can be used manually, semi, or fully automatic. I'm leaning towards Leica as I have had good luck with their manual microtomes. Please, experienced histotech

[Histonet] Thyroglobulin TGB04+TGB05 Not Working in Canine and Feline

Hello, We had to switch sources (to Novus) for Thyroglobulin TGB04+TGB05 and cannot get it to work in canines or felines. Data sheet suggests 1:100, pH6 citrate antigen retrieval. We've also tried zero AR and pH9 EDTA AR, to no avail. Any suggestions? Thanks! Sandy Sandra J.

[Histonet] Leica RM2235 Block Clamp Lever snapped!

Hello all, The block clamp lever of one of our Leica RM2235 microtomes snapped off. The broken end is still in the (threaded?) hole. Any advice or source for fixing/replacing this is most appreciated. Thanks, Sandy Sandra J. Cheasty, HT (ASCP) Histology & Necropsy Supervisor

[Histonet] Softening Calf Hooves

Hello all, I have 1 inch thick cross sections (band saw) of calf hooves. The pathologist has already decaled the bone, and now we have to soften the hooves so she can take thinner sections for processing. * Which is better, potassium hydroxide or sodium hydroxide, and

[Histonet] IHC Control Slide Longevity

Hello all, Do any of you have to cut any of you antibody controls "fresh"? We currently cut the MUM-1 fresh, and the Synaptophysin. (We seldom do a Syanpt., and have found the fresh cut patient tissue has been positive while the not-so-fresh positive control slide is negative.

[Histonet] Used Lab Vision 720D Immunostainer

Hello all, Does anyone know of a company who buys used Histology equipment? Our Lab Vision 720D Immunostainer still works fine, and had a full PM in July 2018. (We purchased a new IHC stainer that is better suited for research.) Cheers, Sandy Sandra J. Cheasty, HT (ASCP)

[Histonet] Ortho-keratotic Mineralizing Odontogenic Cyst

Hello all, Does anyone have any ideas on de-mineralizing an ortho-keratotic, mineralizing, odontogenic cyst? It is so hard the pathologist cannot cut it into small enough pieces to fit into a cassettes. (It's been in decal for days with no change.) I was thinking of taking one

[Histonet] Sakura Tape Coverslipper Advice Needed!

Hello all, Our Sakura tape coverslipper is doing something I've never encountered before - the lever that holds the blade and goes up and down and cuts the tape, has gotten sticky and slow. It will chop down fine, but has a hard time returning to the up position. Before I take

[Histonet] PT Module Spare Part

Hi all, Does anyone know where I can get my hands on some PT module reagent level sensors? (The black bar on the back of each tank.) Our pH 9 AR destroyed it in one tank, and the pH 6 sensor now has some wear and tear also. Otherwise the PT module works great. Thanks! Sandy

[Histonet] CD204 Anti-MSR1 monoclonal, clone SRA-E5

Hello all, Does anyone know where I can get CD204, Anti-MSR1 monoclonal, clone SRA-E5? Thanks! Sandy Sandra J. Cheasty, HT (ASCP) Histology & Necropsy Supervisor UW-Madison, School of Veterinary Medicine ___ Histonet mailing list

[Histonet] Ultra Clean Diluent for IHC

Hi all, It seems Fisher has stopped carrying the "Ultra Clean Diluent 125ml". (Item TA-125-UC). Can anyone recommend someone else who carries it, or a likely replacement? We use the Po-Link 2+Broad HRP detection kit. Cheers! Sandy Sandra J. Cheasty, HT (ASCP) Histology &

[Histonet] IHC Stainer

Hello all, We are in the market for a new IHC stainer. (Our Lab Vision 720 is no longer reliable.) We average about 20 slides a day, but sometimes get research projects that are 50+ slides. Not interested in a closed system; it is for a veterinary histology lab, and we need the

[Histonet] Hard Water and Auto Stainers

Hello all, Thanks for everyone's response to error 90 on our Prisma stainer; it has been cleared! Does anyone use filtered, softened, or DI water in their H stainer to avoid clogging up the system? Thanks! Sandy Sandra J. Cheasty, HT (ASCP) Histology & Necropsy Supervisor

[Histonet] Vacuum Breaker Error on Prisma H Stainer

Hello all, We have been getting an occasional, (maybe once every 20 runs), error 90 on our Prisma stainer. (Vacuum Breaker Error) Has anyone encountered this? Does it have to do with the incoming water lines? I'm trying to avoid a costly visit by a Sakura vetted repair tech.

[Histonet] Lab Vision 720-2D Parts

Hello all, I am in need of some parts for our Lab Vision 720-2D IHC stainer that don't cost an arm and a leg. (Plunger, plastic piece that holds the wide top of the plunger, tubing, stopcock, connections.) If you e-mail me, I can send a picture of exactly what I'm

[Histonet] Grossing Blade for Ophthalmology Globes

Hello all, Can someone recommend an economical disposable blade for grossing eye globes? Sharp enough to slice through sclera smoothly, strong enough to finish the cut through the lens. Cheers! Sandy Sandra J. Cheasty, HT (ASCP) Histology & Necropsy Supervisor UW-Madison, School

[Histonet] Paraformaldehyde Fixed Tissue

Hi all, We are having difficulty sectioning mouse tissue, (brain, spinal cord, liver, and spleen), on paraformaldehyde fixed tissue. Has anyone had issues with paraformaldehyde fixed tissue? They were processed routinely, starting in 10% NBF, with other tissues, and we are

[Histonet] Cardboard File Drawers for Blocks

Hello everyone, Does anyone know of a company that sells the cardboard drawers and drawer dividers for filing blocks, separate from the outer cardboard file that the drawers slide into? We came into possession of 25 outer files with no drawers, which means we

[Histonet] "Supa Mega Clamp"

Hello all, We use the "Supa Mega Clamp" (Ted Pella) for sectioning large tissues in the oversized cassettes. Lately, it seems not to grip as tight as it did when we received it a month or so ago. The literature is little more than photos of how to attach the clamp to our

[Histonet] Pathology Band Saw

Hello, Can anyone recommend a band saw for pathology? We are looking for a new band saw for use in necropsy and research. It has to be a "meat" band saw, as workshop band saws aren't compatible with wet tissue. A table-top model is preferred, and cheaper than a floor model.

[Histonet] Extra Large Cassette Clamps for Microtomes

Hello all, I am looking for opinions on giant block clamps for the Leica RM2235 and Microm HM355S microtomes. * I know CellPath makes one, are their others? * Is it easy to swap them out for a regular clamp, or is it advisable to dedicate one microtome for these

[Histonet] Refurbished Leica RM2135

Hi everyone, I have a newly refurbished Leica RM2135 at my disposal, but the knife holder is for high profile blades. 2 questions. 1-Is it that different using high-profile blades vs. low. (I have used low-profile blades forever.) 2-There is a slot on the bottom of the blade

[Histonet] Long Pipette Sharps Disposal Container

Hello all, Can anyone recommend an economical sharps disposal container for long plastic pipettes? Cheers, Sandy Sandra J. Cheasty, HT (ASCP) Histology & Necropsy Supervisor UW-Madison, School of Veterinary Medicine ___ Histonet

[Histonet] Thyroglobulin Ab-6 (Clone TGB04+TGB05)

Hello all! Does anyone have a source for Thyroglobulin Ab-6 (Clone TGB04+TGB05), 200ug/ml? We use it primarily on canine and feline, (It also works on guinea pigs,). Thanks much! Sandra J. Cheasty, HT (ASCP) Histology & Necropsy Supervisor UW-Madison, School of Veterinary

[Histonet] Microm HM 355S Issues

Hello, We have 2 Microm HM355S motorized microtomes. We use the motor to move the block close to the blade, but turn the wheel manually when sectioning. Both are "jumping" forward on their own occasionally while sectioning, which is unsettling and chunks into the block. One of

[Histonet] Program for organizing and re-naming digital gross photos

Hello all, Picasa, the program we currently use for re-naming and organizing our gross digital photos, will no longer be supported by Google. If anyone can suggest another program for this purpose, please let me know. We don't need any fancy editing of the photos themselves

[Histonet] Animals Need Histologist Too!

Hello All! We are looking for a dedicated Histologist, with at least 1 year of experience in a full service histology lab, to join our Veterinary Pathology team. Our Histology lab serves the Veterinary Medical Teaching Hospital in Madison, Wisconsin, as well as many research needs at the School

[Histonet] Hematoxylin Precipitate

Hello all, Has anyone using Richard Allen Hematoxylin-2 noticed an odd artifact on the slides after using the Hematoxylin for more than a few days on their stainer? We are seeing small spore or pollen-like blue dots here and there on the slides. It is not coming from the water