Re: [Histonet] Bone samples

[Simmons, Christopher via Histonet]

Ion Exchange Decal containers will change your experience forever. 

-Original Message-
From: Gudrun Lang via Histonet  
Sent: Friday, January 26, 2024 3:32 AM
To: 'Chakib Boussahmain' 
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Bone samples

Hi,
In my opinion the hardness of the decalcified blocks is often rather due to the 
paraffin-processing than the residual calcium. Especially when the tissue is 
decalcified really long. The hardness comes from the dehydration and "cooking" 
of collagen fibers. So additional decal will not help reducing the calcium, but 
helps to reintroduce water into the collagen-grid. And this is helpful for 
softening and cutting.
For myself, I often scratch the paraffin on the blocksurface away to face the 
bone directly to the water. Then I let them "swim" on my waterbath, until the 
surface is turned rather milky. After cooling again I cut in very very small 
steps to trim the surface. Sometimes it needs repeated swimming and cooling 
(and patience) to get a rather acceptable section. It is advantageous to pick 
them up on adhesive slides and let them dry in an 60°C oven to get rid of any 
residual water under the section.

Hope this helps
Gudrun

-Ursprüngliche Nachricht-
Von: Chakib Boussahmain via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Gesendet: Mittwoch, 24. Jänner 2024 23:34
An: histonet@lists.utsouthwestern.edu
Betreff: [Histonet] Bone samples


Hi guys,

 I hope this messagefinds you well. I am currently working on a study involving 
bone samples thathave been treated with slow decal and embedded in paraffin. I 
am facingchallenges in obtaining nice sections, and I was wondering if you 
could providesome guidance or recommendations.

Thank you in advance for your help!
Chakib
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[Histonet] Mohs Tech needed

[Simmons, Christopher via Histonet]

All,
The University of Pittsburgh Medical Center, Physicians Services Division, is 
looking for a Mohs tech for immediate hire! If interested, please apply at 
UPMC.com
Thank you!

Chris Simmons, BS, HTL(ASCP)cm, AS
Manager, Dermatopathology and Histology
Medical Arts Building
3708 5th Ave Suite 500.11
Pgh, PA 15213
O: 412.864.3880
C: 412.612.0881
F: 412.864.3890

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Re: [Histonet] comparable eosin

[Simmons, Christopher via Histonet]

We use SelecTech Eosin 515 LT for our derm lab
No complaints

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From: Histology via Histonet 
Sent: Wednesday, September 9, 2020 9:17:19 AM
To: histonet@lists.utsouthwestern.edu 
Subject: [Histonet] comparable eosin

Hi everyone,

We have always got our Eosin from BBC Biochemical.  Well, they were bought out 
by StatLab and I am trying to find a comparable Eosin from what we were using.  
We used "Skin Eosin" catalog #3680 composed of:  water, denatured ethanol, 
eosin y, and glacial acetic acid.  I've done some searching and haven't found 
anything like it.  Is anyone else having this problem or have any advice?

Thanks!

Mehndi Helgren

Dominion Pathology Laboratories
733 Boush Street
Suite 200
Norfolk, VA 23510
757-664-7901

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Re: [Histonet] Tissue processor validation

[Simmons, Christopher via Histonet]

Get your gross lab to provide you with samples representative of what you 
normally process and the same for tissue you use for internally procured 
special stain controls (kidney, lung, etc)
Process as usual and cut and perform specials
If they work: done.

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From: Martha Ward-Pathology via Histonet 
Sent: Wednesday, September 2, 2020 3:51:42 PM
To: histonet@lists.utsouthwestern.edu 
Subject: [Histonet] Tissue processor validation

I am posting this question for my colleague in the Histology lab.   She is 
working on a plan for a new tissue processor validation, including special 
stains, and would like to get input from anyone that has already gone through 
it.This is the first time anyone here has ever been involved in validating 
a new processor.   Current ones are 25+ years.


Any help or advice would be appreciated.   Thanks in advance!

Martha Ward, MT (ASCP) QIHC
Wake Forest Baptist Health
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Re: [Histonet] Reprocessing tissue

[Simmons, Christopher via Histonet]

There are several papers on this. We did a purposeful underprocess and cut and 
stained it, then reprocessed as normal and compared. The results were 
eye-opening in terms of how good it worked, but you need to factor in that the 
second processing will diminish IHC staining quality.

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From: Cassie P. Davis via Histonet 
Sent: Tuesday, September 3, 2019 1:54:13 PM
To: Perl , Alison 
Cc: 'Cassie P. Davis via Histonet' 
Subject: Re: [Histonet] Reprocessing tissue


Thank to those who responded so quickly. It happens maybe once a quarter. It 
happened today because the programs got switched: large tissue processed on 
biopsy program. I've heard of folks doing it this way with the theroy the 
paraffin protects the processed tissue. I'm just old school I guess. I don't 
know if this has been vaildated, I will have to ask our Lead when she gets back 
from vacation.
Thanks for teaching me my something new today!

Cassie Davis
Histology Department
Anatomical Pathology Laboratory
302-575-8095
Email:  cda...@che-east.org





From: Perl , Alison 
Sent: Tuesday, September 3, 2019 12:42 PM
To: Cassie P. Davis 
Cc: 'Cassie P. Davis via Histonet' 
Subject: [External] RE: Reprocessing tissue

Warning:  This email originated from the Internet!
DO NOT CLICK links if the sender is unknown, and NEVER provide your password.

Hi Cassie
How often is this happening? We have done it this way, with no ill effects on 
the processor, but it comes up maybe once every few months - if you have to 
reprocess tissue daily or weekly, I could see it adding up to a problem. We 
melt the block, blot the tissue and cassette with gauze to remove as much 
paraffin as possible, then put in the rack with new work, and usually comes out 
fine.

Alison Perl, HTL(ASCP)CM
Anatomic Pathology Manager
CareMount Medical
(914) 302-8424
ap...@caremount.com


-Original Message-
From: Cassie P. Davis via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Tuesday, September 03, 2019 1:32 PM
To: histonet
Subject: [EXTERNAL] [Histonet] Reprocessing tissue

Has anyone had success with just melting down unfaced, underprocessed blocks 
for reprocessing and sticking them in NBF for processing without taking them 
through the clean cycle? The senior tech is doing this and I am concerned about 
our processor getting paraffin in the NBF and other things.

Cassie Davis

Email:  cda...@che-east.org





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Re: [Histonet] FW: Cerner AB

[Simmons, Christopher via Histonet]

There are so many report combinations it's hard to know where to start.
Pending logs by block status ensure you have cut all the blocks as expected for 
that day. Pending stains by status to know where you are during the day and to 
ensure you have done all ordered by eob.
Overdue logs and stains. Etc all custom by your copath team to your situation.

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From: Morken, Timothy via Histonet 
Sent: Wednesday, April 25, 2018 11:23:28 AM
To: Histonet
Subject: [Histonet] FW: Cerner AB


Jose, do you have Cerner Copath already? If so, and you plan to keep it, AB 
is probably the best option. If you try to use a third-party system like 
Vantage or Cerebro with Copath, Cerner will require that you buy AB anyway as 
the middle-ware. Then you would need to support two separate systems. You also 
may not be able to have two-way communication, that is, writing back to the 
Cerner database from a third party application (for instance, cannot order 
blocks or stains in the third party system and have it be written to the Copath 
database. All blocks/stains must be entered on the Copath side and then they 
are picked up by the third party system).

We have Copath Plus v14 and we have been using AB for 3+ years. It works very 
well, but took a lot of work to implement. We barcode from specimen receipt 
thru grossing, histology, IHC and Special Staining, to block and slide storage 
and tissue storage and disposal.
Cytology as well. All seamlessly. We use the Copath 2D code to run our Leica 
and Ventana stainers without the need to program those stainers - stain orders 
are sent to the stainers from Copath and they "wait" for the 2D-coded slide to 
show up.

We print cassettes at each gross station and slide labels (General Data 
StainerShield) at each microtome when the blocks are scanned. That has worked 
very well.

AB has a "status monitor" that allows you to see how materials move thru the 
lab - for instance it shows how many blocks are in "grossed" status" or "cut" 
status at any given time. You can click on the number on the monitor to see 
exactly which cases are in that status. You can also track all materials of a 
case very easily with one report.

We have pretty much eliminated paper from the lab with this system.

The only downside I have seen is that Copath does not have a good statistics 
reporting tool. They only provide static reports, and "raw data" that must be 
exported to Excel to do any analysis. You can get third party reporting tools 
that tap into the Copath tables and build our your own live stats reports but 
they are pretty expensive.

We have also used AB to make our own materials tracking system for our 
courier system, and chemical tracking in the gross room. It is very flexible in 
that way.

Tim Morken
Pathology Site Manager, Parnassus
Supervisor, Electron Microscopy/Neuromuscular Special Studies
Department of Pathology
UC San Francisco Medical Center

-Original Message-
From: deGuzman, Jose R via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Tuesday, April 24, 2018 2:10 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Cerner AB

Hello Histonetters.

I'm looking for opinions from users of Cerner's Advanced Barcode labeling and 
Tracking Software. I'd like to know your experience working with the software, 
and if you've worked with another software.

Thank you.

Jose de Guzman

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Re: [Histonet] Suggestions for making glutaraldehyde penetrate in between little setae of insects for microscopy

[Simmons, Christopher via Histonet]

Double PPE if you use DMSO


Sent from my iPhone

> On Feb 26, 2016, at 2:41 PM, Jay Lundgren via Histonet 
>  wrote:
> 
> A little alcohol will lower the surface tension of aqueous solutions.  Then
> there's always DMSO.
> 
> 
>   Sincerely,
> 
>Jay A. Lundgren, M.S., HTL (ASCP)
> 
> On Fri, Feb 26, 2016 at 8:15 AM, Jorge A. Santiago-Blay via Histonet <
> histonet@lists.utsouthwestern.edu> wrote:
> 
>> Hello Histonetetrs:
>> 
>> I am trying to fix insect wings for TEM. Less than ideal fixation appears
>> to be caused by abundant setae on the surface of the wings. Those setae,
>> trap air, thus making penetration of the fixative more difficult difficult.
>> Interestingly, also the wings tend to curl upon themselves.
>> 
>> Options re. fixation:
>> 1. Just submerging the insects mechanically does not seem to solve the
>> problem.
>> 
>> 2. We are contemplating submerging and evacuating (intermittently) a few
>> times for 2 minutes (vacuuming)
>> 
>> 3. Are there chemicals that can break the bubbles formed in between the
>> setae?
>> 
>> 4. Any other comments or suggestions?
>> 
>> Options to minimize (ideally eliminate) curling of the wings?
>> 
>> If you have any constructive suggestions, please kindly email it to me
>> directly.
>> 
>> blayjo...@gmail.com
>> 
>> Of course, we intend to begin with new freshly collected insects.
>> 
>> Gratefully,
>> 
>> Jorge
>> 
>> Jorge A. Santiago-Blay, PhD
>> blaypublishers.com
>> 
>> 1. Positive experiences for authors of papers published in *LEB*
>> http://blaypublishers.com/testimonials/
>> 
>> 2. Free examples of papers published in *LEB*:
>> http://blaypublishers.com/category/previous-issues/.
>> 
>> 3. *Guidelines for Authors* and page charges of *LEB*:
>> http://blaypublishers.com/archives/ *.*
>> 
>> 4. Want to subscribe to *LEB*? http://blaypublishers.com/subscriptions/
>> 
>> 
>> http://blayjorge.wordpress.com/
>> http://paleobiology.si.edu/staff/individuals/santiagoblay.cfm
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Re: [Histonet] DIF on paraffin embedded tissue

[Simmons, Christopher via Histonet]

Useless sample
Sorry

Sent from my iPhone

> On Nov 25, 2015, at 11:02 AM, Rene J Buesa via Histonet 
>  wrote:
> 
> No matter WHO to tell you to do WHAT, for IF purposes, that FFPE tissue is 
> USELESS.René 
> 
> 
>On Wednesday, November 25, 2015 10:55 AM, Maryann Deathridge via Histonet 
>  wrote:
> 
> 
> We have a tissue sample that was processed and paraffin embedded.  We 
> URGENTLY need to recover the tissue and perform Immunofluorescence on the 
> sample.
> Does anyone have a procedure.  HELP
>   
> madeathri...@pastnashville.com  
> 
>   
> 
> 
> 
> 
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Re: [Histonet] Are gloves required when cutting FFPE blocks?

[Simmons, Christopher via Histonet]

Never been cited by cap or clia for not wearing gloves
There are hundreds of papers supporting the face that formalin fixed tissues 
will not spread disease all ER docs cite them when you go in for cutting your 
finger and ask about transmission
CJD cases are the exception


Sent from my iPhone

> On Nov 25, 2015, at 10:10 AM, goodwin, diana via Histonet 
>  wrote:
> 
> We were cited for this by CAP.  I need a specific reference to rebut or 
> comply.  Nothing specific to routine paraffin microtomy in CAP checklists.
> 
> dg
> 
> From: Rene J Buesa [mailto:rjbu...@yahoo.com]
> Sent: Wednesday, November 25, 2015 10:04 AM
> To: goodwin, diana; 'histonet@lists.utsouthwestern.edu'
> Subject: Re: [Histonet] Are gloves required when cutting FFPE blocks?
> 
> NOT AT ALL, unless you are sectioning a suspected or known 
> Creutzfeldt-Jakob's disease case. Check CAP regulations.
> René
> 
> 
> On Wednesday, November 25, 2015 9:56 AM, "goodwin, diana via Histonet" 
> > 
> wrote:
> 
> Can anyone provide a reference as to whether or not gloves are required when 
> cutting routine FFPE blocks?
> 
> Diana Goodwin
> UMCPP
> Anatomic Pathology Supervisor
> Office 609-853-6808
> Histology 609-853-16860
> 
> 
> 
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Re: [Histonet] DIF on paraffin embedded tissue

[Simmons, Christopher via Histonet]

By all means share your protocols plz

Sent from my iPhone

> On Nov 25, 2015, at 12:44 PM, Houston, Ronald via Histonet 
>  wrote:
> 
> Obviously it depends on what you are looking at, but I have had much success 
> with DIF on paraffin sections
> 
> 
> Ronnie Houston, MS HT(ASCP)QIHC FIBMS
> Anatomic Pathology Manager
> 700 Children's Drive
> Columbus, OH 43205
> (P) 614-722-5450
> (F) 614-722-2899
> ronald.hous...@nationwidechildrens.org
> www.NationwideChildrens.org
> 
> "Without continual growth and progress, such words as improvement, 
> achievement, and success have no meaning."
> ~ Ben Franklin
> 
> 
> 
> -Original Message-
> From: Rene J Buesa via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
> Sent: Wednesday, November 25, 2015 11:02 AM
> To: madeathri...@pastnashville.com; histonet@lists.utsouthwestern.edu
> Subject: Re: [Histonet] DIF on paraffin embedded tissue
> 
> No matter WHO to tell you to do WHAT, for IF purposes, that FFPE tissue is 
> USELESS.René 
> 
> 
>On Wednesday, November 25, 2015 10:55 AM, Maryann Deathridge via Histonet 
>  wrote:
> 
> 
> We have a tissue sample that was processed and paraffin embedded.  We 
> URGENTLY need to recover the tissue and perform Immunofluorescence on the 
> sample.
> Does anyone have a procedure.  HELP
>   
> madeathri...@pastnashville.com  
> 
>   
> 
> 
> 
> 
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