Re: [Histonet] P 16 Control Slides

Dear Banjo, We perform moderately differentiated squamous cell carcinoma Cervix as positive control for CINec p16 ventana with very good results. Regards Muhammad Tahseen n 2013-12-30 23:36, Adesupo, Adesuyi (Banjo) wrote: Hi, Please we are having trouble getting the P 16 Positive Control

[Histonet] CD 34 antibody abcam (ab963)

do for IHC staining of our samples. Best regards Muhammad Tahseen Sr.Supervisor Histology SKMCHRC Lahore ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] P16

Hi All, I am looking P16 for HP V in Sq.ca of oral cavity, which company and clone will be good. I use Ab cam pathologist not comfortable. Thanks, Muhammad Tahseen Supervisor Histology SKMCHRC Lahore Pakistan ___ Histonet mailing list Histonet

RE: [Histonet] Tissue Processor Validation

Dear All Would any one of you out there,want to share template of Tissue Processor validation forms? Thanks Muhammad Tahseen Histolog Supervisor SKMCHRC Lahore Pakistan On 2013-08-28 19:10, Horn, Hazel V wrote: We did the same as Beth. Hazel Horn ySupervisor of Histolog/Autopsy/Transcription

[Histonet] CD 24 CLONE 528807 and CD 44s Pan

Reconstituted diluted antibody storage temp? 4 Good control tissue? Any other finger tips. Thanks advance. Muhammad Tahseen Senior supervisor Histopathology SKMCHRC Lahore Pakistan ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http

Re: [Histonet] problem with DAB staining

Section should not dry in any stapes. Tahseen On 2013-03-07 22:50, Nicole Cosenza wrote: Hello all: I recently did IHC on paraffin embedded slides via the DAB method. Upon addition of the DAB, the entire section turned brown. I know this is not true staining, as I don't expect a high

Re: [Histonet] sections falling off

in agitated distilled water. The coated slides are then dried for 1 hour, and can be boxed for future use. References:Bancroft sixth edition page 694 Regards Muhammad Tahseen Senior Supervisor Histopathology SKMCHRC Hi Histonetters ! Can anyone suggest how to prevent adult mouse skin sections

Re: [Histonet] Which fixative for eyes

Dear Pedro Louro We are using Alcoholic formalin fixative for ocular (Eye) specimens, Reference Tech Sample (ASAP) HT-2(1995), since last 19 year without facing any problem. Composition: Ethanol absolute 90 ml Formaldehyde con 10 ml Fix for 24 hours Thanks, Muhammad Tahseen Histology Supervisor

[Histonet] validation Form

Good Morning Histoland, I was wondering if anyone has a instument and antibody validation Form they would be willing to share? Thanks, Tahseen ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman

[Histonet] Problem in peloris processor

Dear All! We processed the 3mm biopsies in peloris processor using factory 4 hrs. xylene standard protocol. But the processing was poor (soft) and almost half of the biopsies didn't process well reagent status was ok. Advised require regarding this problem. Regards. Muhammad Tahseen Pathology

[Histonet] Can perform on IHC(P)

Dear All, We have casein kinase sc-6479 santa cruz, can we perform on paraffin embedded section. Thanks Muhammad tahseen ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] collecting chemical waste

Dear MG First when accumulate the chemical waste. Then before chemical waste can be picked up by house keeping staff, a waste tag is required. Muhammad Tahseen SKMCHRC Lahore Pakistan Hello histonetters, Do you folks enforce placing a label on a waste chemical container before collecting any

[Histonet] Bleach the carbons pigments

Hi All, One of our pathologists wants to bleach the carbons pigments from cytology smears.According to Bancroft (page 263) it may be confused with melanin deposition but treatment with bleaching agents will show carbon unaffected. Is there any procedure? Muhammad Tahseen Senior Supervisor

[Histonet] DFA Method for the diagnosis of PCP on sputa.

Dear All, Good Morning, We are going to start DFA Method for the diagnosis of Pneumocystis carinii pneumonitis from Sputa or Tracheal Aspirates from Humans. Would anyone like to share a method/protocol for DFA? Thanks and good weekend to all Muhammad Tahseen MLT (Punjab medical Faculty) MT (Japan

RE: [Histonet] New Reagent Lot Verification

Dear All, Would you like to share format of verification form (new reagent lot/new antibody and new equipment). Thanks, Tahseen Histology supervisor SKMCHRC Lahore Thanks everyone! -Original Message- From: Settembre, Dana [mailto:sette...@umdnj.edu] Sent: Friday, April 13, 2012

Re: [Histonet] Leica Bond verses the Ventana Ultra

We love our Bond too. Muhammad Tahseen Histology supervisor SKMCH RC Lahore Pakistan Need to know histotechs opinion of the Leica Bond verses the Ventana Ultra. judith_par...@memorial.org Judith Pardue This message and accompanying documents are covered by the Electronic

[Histonet] thinprep

We want to purchase a latest model machine for thin prep/sure path along with an auto stainer for pap.stain. Can you share your experience regarding both types of machines, 1) Which type of system is best 2)which machine will provide an auto stainer 3)which model of the recommended machine is best

[Histonet] Peloris Rapid tissue processor

Tahseen Histology Supervisor Deptt. Pathology Shaukat Khanum Cancer Hospital And Research Center Lahore. Pakistan Ph. +92 42 5180725-36 Ext 2369, Fax. +92 42 5180723 e-mail. Histology @t

[Histonet] uncertainity of results in histopathology

Dear all please guide me about uncertainity of results in Histopathology at different steps,including fixation of tissue ,grossing,processing,microtomy,staining,IHC,special staining and interpretation etc. This is the requirement of ISO 15189 CLAUSE:5.6.2 Muhammad Tahseen SKMCHRC Pakistan

[Histonet] OCD rasipy required

Dear All, We required rasipy of the OCD (Frozen section embadding medum). We are out of stock.Any one want to shere. Thanks advanc Tahseen ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo

[Histonet] Protocol for manual processing

Does anyone have a protocol for manual processing for mouse kidneys (ethanol substitute? Thanks In Advance, Muhammad Tahseen SKMCHRC Lahore Pakistan ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman

[Histonet] Measure productivity by skill level

, Muhammad Tahseen Supervisor Histology SKMCHRC Lahore Pakistan ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] AgNORs staining prtocol UVC lamp safeguard

Dear All, 1 Would you like to share your AgNORs staining prtocol with me? 2 We are using Leica CM1850 US Cryostat, we are going to use UVC lamp for disinfection, so what kind of safeguard are recommended other then sliding window has been properly closed. Thanks advance Muhammad Tahseen Senior

[Histonet] Protocol for immunofluorescence staining

Dear All, We are looking protocol for immunofluorescence staining Fibrinogen/FITC and positive control, negative control tissue. Muhammad Tahseen SKMCHRC Lahore,Pakistan ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http

[Histonet] Wear gloves when handling / cutting paraffin blocks.

Dear All, I am looking some kind of references out there that addresses that it is not a requirement to wear gloves when handling / cutting paraffin blocks. Any help would be greatly appreciated Thank in advance, Muhammad Tahseen Senior Supervisor Histology SKMCHRC Pakistan

[Histonet] Ocular fiative

comparison between 10%NBF verses Alcoholic fixative for Ocular (Eye). Thanks, Muhammad Tahseen Histology Supervisor SKMCHRC Lahore Pakistan ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo

[Histonet] carbonic anhydrase 9

Dear all We are locking immuno marker for carbonic anhydrase 9 on p.section for renal cell carcinoma. Thanks advance Muhammad Tahseen Histology Supervisor SKMCHRC Lahore Pakistan ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http

[Histonet] IHC marker for carbonic anhydrase 9

Dear all We are locking immuno marker for carbonic anhydrase 9 on p.section. Thanks advance Muhammad Tahseen Histology Supervisor SKMCHRC Lahore Pakistan ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu

Re: [Histonet] instrument disinfection

We are useing 10% Virkon for gross room instrument. Muhammad Tahseen SKMCHRC Hi All, I need to order disinfecting solution for our grossing instruments. I would like to know what product most labs are using? Thanks for the info. Michele Margiotta BMHMC Histology Supervisor 631-654

[Histonet] Removal of carbon pigment

Hellow all. We have received CT guided FANA smears from lung .The siles are full of carbon particles that mask the under histiocytes and granulomata.W require the removal of carbor particles.Kindly help out to remove them. Muhammad Tahseen Supervisor Histology Javed uz zaman Supervisor Cytology

Re: [Histonet] Frozen Sections Slides per Day Question

Dear Soofia Sissiqui, 25 blocks (12 slides for each block with 3-4 sections)as per Welcan Units code # 5019 entire day ( 8 hours 480 minte) Muhammad Tahseen Histology Supervisor SKMTHRC Lahore Pakistan Hi dear histology experts, I will greatly appreciate if somebody can let me know

Re: [Histonet] General Orientation Checklist

The file is attached with. M. Tahseen, Supervisor Histopathology SKMCHRC, Lahore, Pakistan Good Day Histonetters, Does anyone have a general orientation checklist for histology for new hires that they would be willing to share with me? Thanks in advance for your help, Amy Amy Self

[Histonet] Temperature of the dead body storage refrigerator

Dear all, The temperature of the dead body storage refrigerator was fluctuating up to 6 and 7 centigrade. I am looking referances if any ? Thanks advance Muhammad Tahseen Histology Supervisor SKMCHRC LAHORE,PAKISTAN ___ Histonet mailing list Histonet