Re: [Histonet] P 16 Control Slides
Dear Banjo, We perform moderately differentiated squamous cell carcinoma Cervix as positive control for CINec p16 ventana with very good results. Regards Muhammad Tahseen n 2013-12-30 23:36, Adesupo, Adesuyi (Banjo) wrote: Hi, Please we are having trouble getting the P 16 Positive Control Slides. I will appreciate it, if you guys could tell me where to find one. Thanks, Banjo Adesuyi, BSMT, HT (ASCP), HTL (ASCP), QIHC (ASCP) Histology Supervisor Norman Regional Health System, Norman, OK 73071. Tel: 405- 307- 1145 == CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution, or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender immediately and destroy all copies of this communication and any attachments. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] CD 34 antibody abcam (ab963)
Hi All, We used CD 34 antibody abcam (ab963)with detection kit enVision to stain our samples from rats and rabbits. We need to stain vessels only but macrophages, fibroblast, collagen all are stained in other hand known +ve control (Tonsil) is perform good. Please help us that what should we do for IHC staining of our samples. Best regards Muhammad Tahseen Sr.Supervisor Histology SKMCHRC Lahore ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] P16
Hi All, I am looking P16 for HP V in Sq.ca of oral cavity, which company and clone will be good. I use Ab cam pathologist not comfortable. Thanks, Muhammad Tahseen Supervisor Histology SKMCHRC Lahore Pakistan ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Tissue Processor Validation
Dear All Would any one of you out there,want to share template of Tissue Processor validation forms? Thanks Muhammad Tahseen Histolog Supervisor SKMCHRC Lahore Pakistan On 2013-08-28 19:10, Horn, Hazel V wrote: We did the same as Beth. Hazel Horn ySupervisor of Histolog/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1241 fax hor...@archildrens.org archildrens.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Beth Brinegar Sent: Wednesday, August 28, 2013 8:40 AM To: Rathborne, Toni Cc: histonet@lists.utsouthwestern.edu; dianar...@aol.com Subject: Re: [Histonet] Tissue Processor Validation Yes, we do a few validation runs. Nothing too extreme, however we gross some extra patient tissue and do a small run, then increase the amount of blocks processed until we are ready to run cases for patient diagnosis. It really only takes a week or two. After those blocks are cut, then we compare them to the previous processor results and make changes if neccessary. Beth Brinegar HTL(ASCP) Anatomic Pathology Supervisor Mercy Medical Center Cedar Rapids, IA 52403 On Wed, Aug 28, 2013 at 8:27 AM, Rathborne, Toni trathbo...@somerset-healthcare.com wrote: How do you validate IHC FISH with a new processor? -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto: histonet-boun...@lists.utsouthwestern.edu] On Behalf Of joelle weaver Sent: Wednesday, August 28, 2013 9:23 AM To: dianar...@aol.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Tissue Processor Validation Yes definitely, 5 programs, 25-50 sister, serial sections on tissue that represents patient samples. Joelle Weaver MAOM, HTL (ASCP) QIHC From: dianar...@aol.com Date: Tue, 27 Aug 2013 22:07:19 -0400 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Tissue Processor Validation I am interested in knowing how many of you out there Validate your Tissue Processors before you put them into use and how extreme you go. Diana Ripley ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] CD 24 CLONE 528807 and CD 44s Pan
Hi All I need help who are working with CD 24 CLONE 528807 Catalog Number MAB 5248 (RD Systems) and CD 44s Pan Clone 2cs Catalog Number BBA10 on formalin fixed paraffin embedded sections. 1 Reconstitute at .5ml OR 1 ml with PBS OR DW? 2 What is the dilution factor? 3 Reconstituted diluted antibody storage temp? 4 Good control tissue? Any other finger tips. Thanks advance. Muhammad Tahseen Senior supervisor Histopathology SKMCHRC Lahore Pakistan ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] problem with DAB staining
Section should not dry in any stapes. Tahseen On 2013-03-07 22:50, Nicole Cosenza wrote: Hello all: I recently did IHC on paraffin embedded slides via the DAB method. Upon addition of the DAB, the entire section turned brown. I know this is not true staining, as I don't expect a high yield of my protein of interest. Any suggestions as to why this happened and what to do about it? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] sections falling off
Dear Megha Kumar, Use commercial + charged OR silanized slides. Silanized slides are prepared by cleaning the slides by washing, followed with a rinse in 95% ethanol. 4ml of 3-aminopropyltriethoxy silane is added to 200 ml of acetone and slides are dipped for 30-60 seconds, followed by 60 seconds in agitated distilled water. The coated slides are then dried for 1 hour, and can be boxed for future use. References:Bancroft sixth edition page 694 Regards Muhammad Tahseen Senior Supervisor Histopathology SKMCHRC Hi Histonetters ! Can anyone suggest how to prevent adult mouse skin sections from falling off during in situ hybridization? How to make sections adhere better to slides? I am using poly-lysine coated slides for 5-10 micron thick skin sections. The tissue is fixed in 4% PFA and embedded in paraffin. Please help ! thanks megha -- Megha Kumar, Ph.D. Post-doctoral Research Scholar Sen-Bandyopadhyay Lab Department of Biological Sciences and Bioengineering Indian Institute of Technology Kanpur Kanpur, 208016 India phone - 9695830033 * * * * ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Which fixative for eyes
Dear Pedro Louro We are using Alcoholic formalin fixative for ocular (Eye) specimens, Reference Tech Sample (ASAP) HT-2(1995), since last 19 year without facing any problem. Composition: Ethanol absolute 90 ml Formaldehyde con 10 ml Fix for 24 hours Thanks, Muhammad Tahseen Histology Supervisor SKMCHRC Lahore Pakistan Hello to the eye experts, I am currently working on a large animal (LA) eye project which will give me the flexibility of experimenting with different fixatives. I am currently using Modified Davidson's solution (48 hours) followed by a 10% NBF solution prior to trimming the eyes, embedding , microtomy and HE staining. After literature research, I found that different labs are using: Modified Davidson's solution Davidson's solution 10% NBF Gluteraldehyde Some places inject the eye with fixative before submerging the eye itself. I wanted to get some ideas / preferences from the experts out there so I can cover my entire basis before finalizing my conclusions. Thanks in advance to all, Pedro Louro Group Leader Histology / IHC Our Values: Excellence, Drive, Ownership, Challenge, Teamwork, Respect LEGAL NOTICE This message is confidential and contains information which may be legally privileged. It is intended for the stated addressee(s) only. Access to this email by anyone else is unauthorised. If you are not the intended addressee, any disclosure or copying of the contents of this email or any action taken (or not taken) in reliance on it is unauthorised and is unlawful. If you are not the addressee, please inform the sender immediately and destroy the original message. The data in this email will have been screened for the presence of computer viruses known by the Company at the time the email was produced. The Company cannot guarantee, however, that the email or any attachments are virus free. Delivery of the email will be at the Client's risk. Registered in England No. 1815730 VAT Reg. No. GB425507072 Registered Office: Woolley Road, Alconbury, Huntingdon, Cambridgeshire PE28 4HS ** ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] validation Form
Good Morning Histoland, I was wondering if anyone has a instument and antibody validation Form they would be willing to share? Thanks, Tahseen ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Problem in peloris processor
Dear All! We processed the 3mm biopsies in peloris processor using factory 4 hrs. xylene standard protocol. But the processing was poor (soft) and almost half of the biopsies didn't process well reagent status was ok. Advised require regarding this problem. Regards. Muhammad Tahseen Pathology (Histology) SKMCHRC ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Can perform on IHC(P)
Dear All, We have casein kinase sc-6479 santa cruz, can we perform on paraffin embedded section. Thanks Muhammad tahseen ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] collecting chemical waste
Dear MG First when accumulate the chemical waste. Then before chemical waste can be picked up by house keeping staff, a waste tag is required. Muhammad Tahseen SKMCHRC Lahore Pakistan Hello histonetters, Do you folks enforce placing a label on a waste chemical container before collecting any waste or do you allow the label to be placed on the waste container after the waste is collected? I was taught to place the label on the waste container even before the first drop of waste is collected. MG ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Bleach the carbons pigments
Hi All, One of our pathologists wants to bleach the carbons pigments from cytology smears.According to Bancroft (page 263) it may be confused with melanin deposition but treatment with bleaching agents will show carbon unaffected. Is there any procedure? Muhammad Tahseen Senior Supervisor Histopathology SKMCHRC Lahore Pakistan ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] DFA Method for the diagnosis of PCP on sputa.
Dear All, Good Morning, We are going to start DFA Method for the diagnosis of Pneumocystis carinii pneumonitis from Sputa or Tracheal Aspirates from Humans. Would anyone like to share a method/protocol for DFA? Thanks and good weekend to all Muhammad Tahseen MLT (Punjab medical Faculty) MT (Japan) Senior Supervisor Histopathology SKMCHRC Lahore Pakistan ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] New Reagent Lot Verification
Dear All, Would you like to share format of verification form (new reagent lot/new antibody and new equipment). Thanks, Tahseen Histology supervisor SKMCHRC Lahore Thanks everyone! -Original Message- From: Settembre, Dana [mailto:sette...@umdnj.edu] Sent: Friday, April 13, 2012 11:03 AM To: 'Sebree Linda A'; Rathborne, Toni; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] New Reagent Lot Verification We are saving slides from inspection to inspection, about 2 years. Dana Settembre University Hospital - UMDNJ Newark, NJ -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sebree Linda A Sent: Thursday, April 12, 2012 11:26 AM To: Rathborne, Toni; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] New Reagent Lot Verification We're saving both for at least 2 years. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni Sent: Thursday, April 12, 2012 9:50 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] New Reagent Lot Verification ANP.22760 refers to new lot verification for antibodies and detection. The Evidence of Compliance says that there should be Records of verification of new reagent lots. What is everyone's interpretation of records? Are you saving slides in addition to paper documentation? CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet - This message was secured by ZixCorp(R). CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Leica Bond verses the Ventana Ultra
We love our Bond too. Muhammad Tahseen Histology supervisor SKMCH RC Lahore Pakistan Need to know histotechs opinion of the Leica Bond verses the Ventana Ultra. judith_par...@memorial.org Judith Pardue This message and accompanying documents are covered by the Electronic Communications Privacy Act 18 U.S.C. Sections 2510-2521, and contain information intended for the specified individual(s) only. This information is confidential. If you are not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, copying, or the taking of any action based on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] thinprep
We want to purchase a latest model machine for thin prep/sure path along with an auto stainer for pap.stain. Can you share your experience regarding both types of machines, 1) Which type of system is best 2)which machine will provide an auto stainer 3)which model of the recommended machine is best 4)will they arrange for training of cytotechnologist and pathologist for processing and reportting of specimen 5)which company is providing best after sale service Thanks ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Peloris Rapid tissue processor
Dear All, Our lab is in the process of purchasing our automated tissue processor (Peloris Rapid tissue processor). I would really appreciate comments from anyone who really likes, or dislikes the processor that they are using. Thanks in advance!!! Muhammad Tahseen Histology Supervisor Deptt. Pathology Shaukat Khanum Cancer Hospital And Research Center Lahore. Pakistan Ph. +92 42 5180725-36 Ext 2369, Fax. +92 42 5180723 e-mail. Histology @t skm.org.pk ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] uncertainity of results in histopathology
Dear all please guide me about uncertainity of results in Histopathology at different steps,including fixation of tissue ,grossing,processing,microtomy,staining,IHC,special staining and interpretation etc. This is the requirement of ISO 15189 CLAUSE:5.6.2 Muhammad Tahseen SKMCHRC Pakistan ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] OCD rasipy required
Dear All, We required rasipy of the OCD (Frozen section embadding medum). We are out of stock.Any one want to shere. Thanks advanc Tahseen ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Protocol for manual processing
Does anyone have a protocol for manual processing for mouse kidneys (ethanol substitute? Thanks In Advance, Muhammad Tahseen SKMCHRC Lahore Pakistan ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Measure productivity by skill level
Dear Fellow Histology Professionals, My senior manager give me a task to present a presentation on how to measure and calculate the work lode for manpower (productivity by skill level), we already use Welcan units. Does anyone know who can I prepare? Your input would be really appreciated, Muhammad Tahseen Supervisor Histology SKMCHRC Lahore Pakistan ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] AgNORs staining prtocol UVC lamp safeguard
Dear All, 1 Would you like to share your AgNORs staining prtocol with me? 2 We are using Leica CM1850 US Cryostat, we are going to use UVC lamp for disinfection, so what kind of safeguard are recommended other then sliding window has been properly closed. Thanks advance Muhammad Tahseen Senior Supervisore Histopathology SKMCH RC Lahore Pakistan. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Protocol for immunofluorescence staining
Dear All, We are looking protocol for immunofluorescence staining Fibrinogen/FITC and positive control, negative control tissue. Muhammad Tahseen SKMCHRC Lahore,Pakistan ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Wear gloves when handling / cutting paraffin blocks.
Dear All, I am looking some kind of references out there that addresses that it is not a requirement to wear gloves when handling / cutting paraffin blocks. Any help would be greatly appreciated Thank in advance, Muhammad Tahseen Senior Supervisor Histology SKMCHRC Pakistan ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Ocular fiative
HI all, We are using Alcoholic formalin fixative for ocular (Eye) specimens, Reference Tech Sample (ASCP) HT-2(1995), since last 15 year without facing any problem. Now one of our pathologists (New joiner) wants to fix Ocular in 10%NBF. I would lick to know how are doing others, and is there any comparison between 10%NBF verses Alcoholic fixative for Ocular (Eye). Thanks, Muhammad Tahseen Histology Supervisor SKMCHRC Lahore Pakistan ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] carbonic anhydrase 9
Dear all We are locking immuno marker for carbonic anhydrase 9 on p.section for renal cell carcinoma. Thanks advance Muhammad Tahseen Histology Supervisor SKMCHRC Lahore Pakistan ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] IHC marker for carbonic anhydrase 9
Dear all We are locking immuno marker for carbonic anhydrase 9 on p.section. Thanks advance Muhammad Tahseen Histology Supervisor SKMCHRC Lahore Pakistan ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] instrument disinfection
We are useing 10% Virkon for gross room instrument. Muhammad Tahseen SKMCHRC Hi All, I need to order disinfecting solution for our grossing instruments. I would like to know what product most labs are using? Thanks for the info. Michele Margiotta BMHMC Histology Supervisor 631-654-7192 DISCLAIMER: This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to which they are addressed. This communication may contain material protected by the attorney-client privilege. If you are not the intended recipient or the person responsible for delivering the e-mail to the intended recipient, be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the sender via return e-mail or call Brookhaven Memorial Hospital Medical Center at (631) 654-7282. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Removal of carbon pigment
Hellow all. We have received CT guided FANA smears from lung .The siles are full of carbon particles that mask the under histiocytes and granulomata.W require the removal of carbor particles.Kindly help out to remove them. Muhammad Tahseen Supervisor Histology Javed uz zaman Supervisor Cytology SKMT PAKISTAN LAHORE ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Frozen Sections Slides per Day Question
Dear Soofia Sissiqui, 25 blocks (12 slides for each block with 3-4 sections)as per Welcan Units code # 5019 entire day ( 8 hours 480 minte) Muhammad Tahseen Histology Supervisor SKMTHRC Lahore Pakistan Hi dear histology experts, I will greatly appreciate if somebody can let me know the estimated number of slides (with 3 sections per slide) per day on average a lab technician can cut (of frozen tissue). I am a lab technician ( not a histotech) and work alone in a highly complex testing specialzed low volume dermatology lab.My job description includes 30 % of immunohistochemistry related dutites. I cut skin frozen sections and do immunohistochemistry manually for several years . My routiene panel consists of 12 antibodies for T-cells surface markers. Ocassionally I add another panel of 8 antibodies for B-Cells. I am very slow in cutting sections and strugle a lot to get good sections. If I spend entire day ( 8 hours) just cutting 3-4 section on each slide with slow speed. What number of slides should be considered as efficent cutting? What number of blocks (12 slides for each block with 3-4 sections) should I finish in one day? Help me please if you can. Thanks. Soofia ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] General Orientation Checklist
The file is attached with. M. Tahseen, Supervisor Histopathology SKMCHRC, Lahore, Pakistan Good Day Histonetters, Does anyone have a general orientation checklist for histology for new hires that they would be willing to share with me? Thanks in advance for your help, Amy Amy Self Georgetown Hospital System 843-527-7179 NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Temperature of the dead body storage refrigerator
Dear all, The temperature of the dead body storage refrigerator was fluctuating up to 6 and 7 centigrade. I am looking referances if any ? Thanks advance Muhammad Tahseen Histology Supervisor SKMCHRC LAHORE,PAKISTAN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
