Dear colleagues
I am not ok with staining of Melanosome (HMB45) Ventana using BenchMark GX The expiry due date still valid and I follow the staining protocol from the pamphlet but no good stain show even on the melanoma control sections. The protocol is : Recommended Staining Protocol for CONFIRM anti-Melanosome (HMB45) with ultraView Universal Red on BenchMark XT. CONFIRM anti-Melanosome (HMB45) Mouse Monoclonal Primary Antibody, Catalog Number 790-4366 Procedure Type Method BenchMark XT Deparaffinization Selected Cell Conditioning (Antigen Unmasking) None required Enzyme (Protease) None required Antibody (Primary) Approximately 8 minutes, 37 °C Counterstain Hematoxylin II, 4 minutes Post Counterstain Bluing Reagent, 4 minutes Best Regards, Jamal M. Al Rowaihi Anatomic Pathology Supervisor | Al Borg Medical Laboratories | Mobile +966 503629832| <mailto:j.rowa...@alborglaboratories.com> j.rowa...@alborglaboratories.com Palestine St, Al Rajhi Building, P.O. Box 52817, Jeddah 21573, KSA | Phone: +966 12 670 0099 | Fax: +966 12 676 4984 | <http://www.alborglaboratories.com/> www.alborglaboratories.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
