Re: [Histonet] Caspase 3 and Tunel Assay

[Elizabeth Chlipala via Histonet]

Ronnie 

We have these protocols in place for porcine tissue.  We use the roche kit for 
tunel 11684809910 and for CC3 we use the cell signaling antibody 9664.

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Laboratory, LLC
PO Box 18592
Boulder, CO 80308
(303) 682-3949 office
(303) 682-9060 fax
(303) 881-0763 cell
l...@premierlab.com
www.premierlab.com

Ship to Address:

Premier Laboratory, LLC
1567 Skyway Drive, Unit E
Longmont, CO 80504

-Original Message-
From: Houston, Ronald via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Tuesday, August 16, 2016 9:45 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Caspase 3 and Tunel Assay

Is anyone working on this just now? And more specifically on the pig species?

Any pointers would be appreciated
Thanks

Ronnie Houston, MS HT(ASCP)QIHC FIBMS
Anatomic Pathology Manager
Laboratory Services
700 Children's Drive
Columbus, OH 43205
(P) 614-722-5450
(F) 614-722-2899
ronald.hous...@nationwidechildrens.org<mailto:ronald.hous...@nationwidechildrens.org>
www.NationwideChildrens.org<http://www.nationwidechildrens.org/>

"Without continual growth and progress, such words as improvement, achievement, 
and success have no meaning."
~ Ben Franklin

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[Histonet] Caspase 3 and Tunel Assay

[Houston, Ronald via Histonet]

Is anyone working on this just now? And more specifically on the pig species?

Any pointers would be appreciated
Thanks

Ronnie Houston, MS HT(ASCP)QIHC FIBMS
Anatomic Pathology Manager
Laboratory Services
700 Children's Drive
Columbus, OH 43205
(P) 614-722-5450
(F) 614-722-2899
ronald.hous...@nationwidechildrens.org
www.NationwideChildrens.org

"Without continual growth and progress, such words as improvement, achievement, 
and success have no meaning."
~ Ben Franklin

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[Histonet] Caspase 3

[sgoebel]

I know I have asked about this in the past, but I'm finally getting
around to optimizing my antibody (Caspase3).  First, tonsil will be a
good control?

Second, I ordered it from Abcam that is telling me the optimal dilution
is between 1/10 and 1/20.  This seems a little crazy?  I looked at some
reviews for the antibody and other people seem to have used this
dilution...wow!  This is going to be an expensive antibody to use!!!

 

Anyhoo, main thing is want to make sure that tonsil will be ok for a
positive control =)

Stay warm my fellow Texans...it'll be 80 on Sunday!!

 

Sarah Goebel, BA, HT(ASCP)

Histotechnologist

Mirna Therapeutics

2150 Woodward Street

Suite 100

Austin, Texas  78744

(512)901-0900 ext. 6912

 

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[Histonet] caspase 3 ihc on rabbit tissue

[Nejat Yilmaz]

Dear Friends,

We are planning to perform active caspase 3 immunohistochemistry on FFPE 
rabbit cornea tissues. Most antibodies I have found are not working on 
rabbit.

Any suggestion???
Thanks in advance...

Dr. Necat Yilmaz
Mersin University
TURKEY 



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Re: [Histonet] Caspase 3 staining in frozen tissue

[TF]

acetone fixed - PFA fixation again?
I think one step is enough


2009-08-26 



TF 



发件人： lau...@conxis.com 
发送时间： 2009-08-26  03:04:41 
收件人： histonet@lists.utsouthwestern.edu 
抄送： 
主题： [Histonet] Caspase 3 staining in frozen tissue 
Hello everyone, 
Is there anyone out there doing Caspase 3 IHC on frozen tissue?  I am 
attempting to restandardize our protocol because the antibody we were using has 
stopped working.  We are using acetone fixed frozen human tonsil. 4% PFA 
postfixation for 10 minutes.  Using a rabbit polyclonal antibody from Abcam and 
Dako reagents on an autostainer.
Thanks for your ideas because mine aren't staining :-(.
Laurie 
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[Histonet] Caspase 3 staining in frozen tissue

[laurie]

Hello everyone, 

Is there anyone out there doing Caspase 3 IHC on frozen tissue?  I am 
attempting to restandardize our protocol because the antibody we were using has 
stopped working.  We are using acetone fixed frozen human tonsil. 4% PFA 
postfixation for 10 minutes.  Using a rabbit polyclonal antibody from Abcam and 
Dako reagents on an autostainer.

Thanks for your ideas because mine aren't staining :-(.

Laurie 

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