[Histonet] Embedding cells in Paraffin

[Mike Tighe]

Has anyone tried to embed cells grown in tissue culture? I am trying to put 
some tissue culture cells through same stress as tissue would go through. 
Fixation, dehydration, and heat. Any ideas? I could re-suspend in OCT and then 
fix for extended time with NBF but that doesn't quite seem fair.



Thanks for any ideas!
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RE: [Histonet] Embedding cells in Paraffin

[joelle weaver]

I spun down the cultured cells and then used a product called histogel. It kept 
them protected through the VIP, paraffin embedded like cell block buttons and 
was able to cut regular paraffin sections. I believe that I purchased the 
histogel from American Mastertech. 




Joelle Weaver MAOM, HTL (ASCP) QIHC
 
 From: mti...@trudeauinstitute.org
 To: histonet@lists.utsouthwestern.edu
 Date: Mon, 2 Dec 2013 17:06:24 +
 Subject: [Histonet] Embedding cells in Paraffin
 
 Has anyone tried to embed cells grown in tissue culture? I am trying to put 
 some tissue culture cells through same stress as tissue would go through. 
 Fixation, dehydration, and heat. Any ideas? I could re-suspend in OCT and 
 then fix for extended time with NBF but that doesn't quite seem fair.
 
 
 
 Thanks for any ideas!
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 Histonet mailing list
 Histonet@lists.utsouthwestern.edu
 http://lists.utsouthwestern.edu/mailman/listinfo/histonet
  
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