Re: [Histonet] IHC validation after a new tissue processer is installed

[Greg Dobbin via Histonet]

Hi martha,
Prognostic markers must be re-validated (Eg.s Breast markers and CD117) as
you described.
Every Ab in your menu should be  tested (as you would for a new a new lot)
and do not forget to validate your H (with various tissue types) and SS
as well. For the H, if possible do side-by-side comparisons between old
and new processors (the downside of what is otherwise an exciting time!).
Greg

-- 
*Greg Dobbin*
1205 Pleasant Grove Rd
RR#2 York,
PE  C0A 1P0


*Everything in moderation...even moderation itself**!*
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[Histonet] IHC validation after a new tissue processer is installed

[Martha Ward-Pathology via Histonet]

Hello all,

After a very long time our histology department is getting a new tissue 
processor, and hopefully several more in the near future.   Of course for IHC 
that means a revalidation/reverification of our IHC stains.  Since I have not 
had to do this before I wanted to get some guidance on how to go about doing 
this.  I feel like I have to completely revalidate the ER, PR and Her2 (20 
positive, 20 negative) but wasn't sure about the other antibodies.   We have a 
menu of around 130do I have to test every one of them or can we chose a 
representative sample? How many antibodies would you suggest?   How many 
positive and negative cases of each should we run?   It says it is left up to 
the medical director but with our CAP inspection next spring we want to make 
sure we will be fully prepared.What have others done?

Thank you in advance for your help with this.


Martha Ward, MT (ASCP) QIHC
Wake Forest Baptist Medical Center
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