Re: [Histonet] Microtome calibration
We have a service contract with an annual PM. The aligments and calibration are done then. Pam - Original Message - From: Michael Ann Jones mjo...@metropath.com To: Ronda Mire rm...@cvpath.org, Denise Long denise.l...@uconn.edu Cc: histonet@lists.utsouthwestern.edu Sent: Wednesday, July 16, 2014 8:07:37 AM Subject: Re: [Histonet] Microtome calibration We do ours by hand? You mean line them up or PM? Michael Ann Jones, HT (ASCP) Histology Manager Metropath 7444 W. Alaska Dr. #250 Lakewood, CO 80226 303.634.2511 mjo...@metropath.com On 7/15/14, 1:55 PM, Ronda Mire rm...@cvpath.org wrote: Hi Denise, Check with the company that does your PM service. This may be something that is already done during preventative maintenance. On Jul 15, 2014, at 3:02 PM, Long, Denise denise.l...@uconn.edu wrote: Hi Everyone, During a recent inspection we were asked by the inspection team to start calibrating our microtomes. Anyone know of a service vendor that will do this? Referrals, including self-referrals would be greatly appreciated. Thank, Denise M. Long, MS, HTL (ASCP), QIHC University of Connecticut Dept. of Pathobiology and Veterinary Sciences Connecticut Veterinary Medical Diagnostic Laboratory 61 N. Eagleville Road, Unit 3089 Storrs, Connecticut 06269-3089 (860) 486-0851 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Microtome calibration
Hi Everyone, During a recent inspection we were asked by the inspection team to start calibrating our microtomes. Anyone know of a service vendor that will do this? Referrals, including self-referrals would be greatly appreciated. Thank, Denise M. Long, MS, HTL (ASCP), QIHC University of Connecticut Dept. of Pathobiology and Veterinary Sciences Connecticut Veterinary Medical Diagnostic Laboratory 61 N. Eagleville Road, Unit 3089 Storrs, Connecticut 06269-3089 (860) 486-0851 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Microtome calibration
Hi Denise, Check with the company that does your PM service. This may be something that is already done during preventative maintenance. On Jul 15, 2014, at 3:02 PM, Long, Denise denise.l...@uconn.edu wrote: Hi Everyone, During a recent inspection we were asked by the inspection team to start calibrating our microtomes. Anyone know of a service vendor that will do this? Referrals, including self-referrals would be greatly appreciated. Thank, Denise M. Long, MS, HTL (ASCP), QIHC University of Connecticut Dept. of Pathobiology and Veterinary Sciences Connecticut Veterinary Medical Diagnostic Laboratory 61 N. Eagleville Road, Unit 3089 Storrs, Connecticut 06269-3089 (860) 486-0851 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Microtome calibration
We do ours by hand? You mean line them up or PM? Michael Ann Jones, HT (ASCP) Histology Manager Metropath 7444 W. Alaska Dr. #250 Lakewood, CO 80226 303.634.2511 mjo...@metropath.com On 7/15/14, 1:55 PM, Ronda Mire rm...@cvpath.org wrote: Hi Denise, Check with the company that does your PM service. This may be something that is already done during preventative maintenance. On Jul 15, 2014, at 3:02 PM, Long, Denise denise.l...@uconn.edu wrote: Hi Everyone, During a recent inspection we were asked by the inspection team to start calibrating our microtomes. Anyone know of a service vendor that will do this? Referrals, including self-referrals would be greatly appreciated. Thank, Denise M. Long, MS, HTL (ASCP), QIHC University of Connecticut Dept. of Pathobiology and Veterinary Sciences Connecticut Veterinary Medical Diagnostic Laboratory 61 N. Eagleville Road, Unit 3089 Storrs, Connecticut 06269-3089 (860) 486-0851 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Microtome calibration
Could they have meant an aligner - something like this? http://www.newcomersupply.com/products/microtome-cryostat-aligners?page=M#1130 Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.we...@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Long, Denise Sent: Tuesday, July 15, 2014 3:02 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Microtome calibration Hi Everyone, During a recent inspection we were asked by the inspection team to start calibrating our microtomes. Anyone know of a service vendor that will do this? Referrals, including self-referrals would be greatly appreciated. Thank, Denise M. Long, MS, HTL (ASCP), QIHC University of Connecticut Dept. of Pathobiology and Veterinary Sciences Connecticut Veterinary Medical Diagnostic Laboratory 61 N. Eagleville Road, Unit 3089 Storrs, Connecticut 06269-3089 (860) 486-0851 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] microtome calibration
Have you tried altering the angle of the knife? Kemlo Rogerson e-mail kemloroger...@nhs.net if not at work. DD 01934 647057 or extension 3311 Mob 07749 754194; Embrace uncertainty. Hard problems rarely have easy solutions. --Jonah Lehrer This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Dianne Holmes Sent: 02 June 2009 22:44 To: Histonet Subject: [Histonet] microtome calibration Has anyone had problems with the calibration of an A/O Spencer sliding microtome? I have used this one for years and now I am getting 'thick-thin' sections? I thought maybe the accumulation of oils in the mechanics of it have slowed things down. Can WD40 be applied without adverse effects? I certainly would not attempt opening up all the gears!! Any suggestions would be appreciated or if a medical equipment repairman is reading this - I really need this thing fixed. Individuals who have received this information in error or are not authorized to receive it must promptly return or dispose of the information and notify the sender. Those individuals are hereby notified that they are strictly prohibited from reviewing, forwarding, printing, copying, distributing or using this information in any way. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] microtome calibration
Diane: I provide microtome service calibration etc. Please email me if I can be of service. References upon request. p...@firnschild.com Paul Paul M. Firnschild QA Support Services, Inc. 404.291.3715 -Original Message- From: Dianne Holmes [mailto:dhol...@anatomy.umsmed.edu] Sent: Tuesday, June 02, 2009 5:44 PM To: Histonet Subject: [Histonet] microtome calibration Has anyone had problems with the calibration of an A/O Spencer sliding microtome? I have used this one for years and now I am getting 'thick-thin' sections? I thought maybe the accumulation of oils in the mechanics of it have slowed things down. Can WD40 be applied without adverse effects? I certainly would not attempt opening up all the gears!! Any suggestions would be appreciated or if a medical equipment repairman is reading this - I really need this thing fixed. Individuals who have received this information in error or are not authorized to receive it must promptly return or dispose of the information and notify the sender. Those individuals are hereby notified that they are strictly prohibited from reviewing, forwarding, printing, copying, distributing or using this information in any way. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] microtome calibration
Has anyone had problems with the calibration of an A/O Spencer sliding microtome? I have used this one for years and now I am getting 'thick-thin' sections? I thought maybe the accumulation of oils in the mechanics of it have slowed things down. Can WD40 be applied without adverse effects? I certainly would not attempt opening up all the gears!! Any suggestions would be appreciated or if a medical equipment repairman is reading this - I really need this thing fixed. Individuals who have received this information in error or are not authorized to receive it must promptly return or dispose of the information and notify the sender. Those individuals are hereby notified that they are strictly prohibited from reviewing, forwarding, printing, copying, distributing or using this information in any way. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Microtome calibration
Dear all Is anyone practising microtome calibration for thickness of sections cut? Our GLP auditors are frequently asking for it. They also suggest that automatic tissue processor time in each reagent should be calibrated. any comments Regards Dr Girish India Disclaimer This message contains legally privileged and/or confidential information. If you are not the intended recipient(s), or employee or agent responsible for delivery of this message to the intended recipient(s), you are hereby notified that any dissemination, distribution or copying of this e-mail message is strictly prohibited. If you have received this message in error, please immediately notify the sender and delete this e-mail message from your computer. WARNING: Computer viruses can be transmitted via email. The recipient should check this email and any attachments for the presence of viruses. The company accepts no liability for any damage caused by any virus transmitted by this email. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Microtome calibration
Dear Dr. Girish, I agree with rene, if the qualitiy of the cuts is fine it doesn't matter if they are 1µm or 2.3µm thick. But I'm highly interested in how your GLP auditors want to calibrate the thickness of the section cuts. Are you supposed to measure the slides after cutting?! Before or after stretching in the water bath?! If they have a protocol how to do this I would be happy if they can share it with us. Frauke Quoting Rene J Buesa [EMAIL PROTECTED]: Dr. Girish: BOTH requirements are rubbish invented by bureaucrats with lots of time in their hands and trying to appear concerned and knowledgeable. Thickness is not necessary as long as the section is diagnostically useful or if some quantitative method as to the intensity is done in which case thickness = amount of matter, and would influence the outcome of the quantitative process. Timing the tissue processor is also totally irrelevant because it does not really matter a few minutes each way during a processing protocol. More important would be to keep a record of the fixation time that is the only step really critical in tissue processing. René J. --- On Fri, 10/31/08, [EMAIL PROTECTED] [EMAIL PROTECTED] wrote: From: [EMAIL PROTECTED] [EMAIL PROTECTED] Subject: [Histonet] Microtome calibration To: histonet@lists.utsouthwestern.edu Date: Friday, October 31, 2008, 2:49 AM Dear all Is anyone practising microtome calibration for thickness of sections cut? Our GLP auditors are frequently asking for it. They also suggest that automatic tissue processor time in each reagent should be calibrated. any comments Regards Dr Girish India ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message was sent using IMP, the Internet Messaging Program. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Microtome calibration
Frauke: That calibration is ussually done by measuring the advance mechanism of the block holder and how many µm the block moves towards the blade, but that is rubbish. René J. --- On Fri, 10/31/08, Dr. med. Frauke Neff [EMAIL PROTECTED] wrote: From: Dr. med. Frauke Neff [EMAIL PROTECTED] Subject: Re: [Histonet] Microtome calibration To: [EMAIL PROTECTED] Cc: histonet@lists.utsouthwestern.edu, [EMAIL PROTECTED] Date: Friday, October 31, 2008, 9:41 AM Dear Dr. Girish, I agree with rene, if the qualitiy of the cuts is fine it doesn't matter if they are 1µm or 2.3µm thick. But I'm highly interested in how your GLP auditors want to calibrate the thickness of the section cuts. Are you supposed to measure the slides after cutting?! Before or after stretching in the water bath?! If they have a protocol how to do this I would be happy if they can share it with us. Frauke Quoting Rene J Buesa [EMAIL PROTECTED]: Dr. Girish: BOTH requirements are rubbish invented by bureaucrats with lots of time in their hands and trying to appear concerned and knowledgeable. Thickness is not necessary as long as the section is diagnostically useful or if some quantitative method as to the intensity is done in which case thickness = amount of matter, and would influence the outcome of the quantitative process. Timing the tissue processor is also totally irrelevant because it does not really matter a few minutes each way during a processing protocol. More important would be to keep a record of the fixation time that is the only step really critical in tissue processing. René J. --- On Fri, 10/31/08, [EMAIL PROTECTED] [EMAIL PROTECTED] wrote: From: [EMAIL PROTECTED] [EMAIL PROTECTED] Subject: [Histonet] Microtome calibration To: histonet@lists.utsouthwestern.edu Date: Friday, October 31, 2008, 2:49 AM Dear all Is anyone practising microtome calibration for thickness of sections cut? Our GLP auditors are frequently asking for it. They also suggest that automatic tissue processor time in each reagent should be calibrated. any comments Regards Dr Girish India ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message was sent using IMP, the Internet Messaging Program. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Microtome calibration
We have a GLP person who is not familiar with Histology at all. We have had to educate her about these issues and may other things. Calibration finally came down to having a PM on the units every year. After she understood all the things we do with a section during the cutting phase and pick up that would alter a true measurement. We also do MMA sections on the microtome and even those are next to impossible to measure due to some stretching of the section during pick up and drying. Oh Yes, the drying of the slides really put her in a tail spin as this was just not allowing the sections to stay the same as when they were picked up. Sometimes a long talk and demonstration is the only way to make the point that we are not standardized like clinical chemistry etc. Pamela A Marcum University of Pennsylvania School of Veterinary Medicine Comparative Orthopedic Laboratory (CORL) 382 W Street Rd Kennett Square PA 19438 610-925-6278 -Original Message- From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] On Behalf Of Rene J Buesa Sent: Friday, October 31, 2008 10:08 AM To: Dr. med. Frauke Neff Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Microtome calibration Frauke: That calibration is ussually done by measuring the advance mechanism of the block holder and how many µm the block moves towards the blade, but that is rubbish. René J. --- On Fri, 10/31/08, Dr. med. Frauke Neff [EMAIL PROTECTED] wrote: From: Dr. med. Frauke Neff [EMAIL PROTECTED] Subject: Re: [Histonet] Microtome calibration To: [EMAIL PROTECTED] Cc: histonet@lists.utsouthwestern.edu, [EMAIL PROTECTED] Date: Friday, October 31, 2008, 9:41 AM Dear Dr. Girish, I agree with rene, if the qualitiy of the cuts is fine it doesn't matter if they are 1µm or 2.3µm thick. But I'm highly interested in how your GLP auditors want to calibrate the thickness of the section cuts. Are you supposed to measure the slides after cutting?! Before or after stretching in the water bath?! If they have a protocol how to do this I would be happy if they can share it with us. Frauke Quoting Rene J Buesa [EMAIL PROTECTED]: Dr. Girish: BOTH requirements are rubbish invented by bureaucrats with lots of time in their hands and trying to appear concerned and knowledgeable. Thickness is not necessary as long as the section is diagnostically useful or if some quantitative method as to the intensity is done in which case thickness = amount of matter, and would influence the outcome of the quantitative process. Timing the tissue processor is also totally irrelevant because it does not really matter a few minutes each way during a processing protocol. More important would be to keep a record of the fixation time that is the only step really critical in tissue processing. René J. --- On Fri, 10/31/08, [EMAIL PROTECTED] [EMAIL PROTECTED] wrote: From: [EMAIL PROTECTED] [EMAIL PROTECTED] Subject: [Histonet] Microtome calibration To: histonet@lists.utsouthwestern.edu Date: Friday, October 31, 2008, 2:49 AM Dear all Is anyone practising microtome calibration for thickness of sections cut? Our GLP auditors are frequently asking for it. They also suggest that automatic tissue processor time in each reagent should be calibrated. any comments Regards Dr Girish India ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message was sent using IMP, the Internet Messaging Program. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
