[Histonet] Mycoplasma replies

[Cheryl Crowder via Histonet]

I want to thank those who responded to my inquiry about staining
mycoplasma.  The researcher has access to DiffQuik stains in particular and
is going to.try that first.  Wish us luck.z

Cheryl Cprowder
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[Histonet] Mycoplasma staining

[Cheryl Crowder via Histonet]

I work with a researcher who has mycoplasma species cells on agar.  He does
not have access to a fluorescent microscope.   Does anyone know of a
technique using regular stain?
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Re: [Histonet] Mycoplasma

[Julio Benavides via Histonet]

No, it won´t. You better think on IHC for labelling mycoplasma.

cheers

Julio

El 05/04/2016 a las 20:12, Bernice Frederick via Histonet escribió:

Hello all,
Will PAS stain mycoplasma?
Bernice

Bernice Frederick HTL (ASCP)
Senior Research Tech
Pathology Core Facility
Robert. H. Lurie Cancer Center
Northwestern University
710 N Fairbanks Court
Olson 8-421
Chicago,IL 60611
312-503-3723
b-freder...@northwestern.edu

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[Histonet] Mycoplasma

[Bernice Frederick via Histonet]

Hello all,
Will PAS stain mycoplasma?
Bernice

Bernice Frederick HTL (ASCP)
Senior Research Tech
Pathology Core Facility
Robert. H. Lurie Cancer Center
Northwestern University
710 N Fairbanks Court
Olson 8-421
Chicago,IL 60611
312-503-3723
b-freder...@northwestern.edu

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Re: [Histonet] Mycoplasma

[Normington Lacy via Histonet]

I do not believe it will stain with PAS.  The bacteria lack a cell wall.

-Original Message-
From: Bernice Frederick via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Tuesday, April 05, 2016 1:12 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Mycoplasma

Hello all,
Will PAS stain mycoplasma?
Bernice

Bernice Frederick HTL (ASCP)
Senior Research Tech
Pathology Core Facility
Robert. H. Lurie Cancer Center
Northwestern University
710 N Fairbanks Court
Olson 8-421
Chicago,IL 60611
312-503-3723
b-freder...@northwestern.edu<mailto:b-freder...@northwestern.edu>

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[Histonet] Mycoplasma stain

[Erin Sarricks]

Hi Histonet-

Does anyone know of a good stain for mycoplasma?  Our pathologist wanted to
try an overnight Giemsa stain, but I wanted to check and see if anyone knew
of another stain that might work well.  Thanks!


Erin Sarricks, HT (ASCP)

Histology Laboratory Technician

USAMRICD Comparative Pathology Branch

Office: Bldg E-3081 Room 178

E-mail: erin.p.sarri...@us.army.mil

Phone: 410-436-1967
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Re: [Histonet] Mycoplasma

[Rene J Buesa]

Roberta:
Sigma-Aldrich offer a staining kit for Mycoplasma.
Their cat. # MYC1
René J.

--- On Tue, 5/18/10, Roberta Horner r...@psu.edu wrote:


From: Roberta Horner r...@psu.edu
Subject: [Histonet] Mycoplasma
To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu
Date: Tuesday, May 18, 2010, 8:42 AM


Does anyone know of a special stain for mycoplasma?  I searched all my books 
and can't find anything.
Thank you
Roberta Horner HT/HTL
Animal Diagnostic Lab
Penn  State University
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[Histonet] Mycoplasma bovis

[Breeden, Sara]

My boss has thought up a new project for me so I need to know what I
need to know about Mycoplasma bovis for IHC.  He wants me to cost out
the procedure.  I normally do only TWO IHCs - CWD and BVD - so I'll be
charting new mental territory with this one.  Can anyone guide me on
where to start figuring this out?  Where do I get antibody? Is there a
pre-existing protocol for the Ventana NexES? I don't believe in
re-inventing the wheel if I don't need to.  Thanks!

 

Sally Breeden, HT(ASCP)

NM Dept. of Agriculture

Veterinary Diagnostic Services

PO Box 4700

Albuquerque, NM  87106

505-841-2576

 

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Re: [Histonet] Mycoplasma bovis

[Jenee . S . Odani]

Hi there, 

Check out this database. It contains a contact list for the labs that are 
currently doing the Myco bovis IHC. 

http://ihc.sdstate.org/

-J

Jenee S. Odani, D.V.M., Dipl. ACVP
Veterinary Medical Officer
Hawaii State Veterinary Laboratory/DAI
99-941 Halawa Valley Street, Aiea, HI, 96701
Phone: (808) 483-7131/Fax: (808) 483-7110





Breeden, Sara sbree...@nmda.nmsu.edu 
Sent by: histonet-boun...@lists.utsouthwestern.edu
08/06/2009 08:12 AM

To
histonet@lists.utsouthwestern.edu
cc

Subject
[Histonet] Mycoplasma bovis






My boss has thought up a new project for me so I need to know what I
need to know about Mycoplasma bovis for IHC.  He wants me to cost out
the procedure.  I normally do only TWO IHCs - CWD and BVD - so I'll be
charting new mental territory with this one.  Can anyone guide me on
where to start figuring this out?  Where do I get antibody? Is there a
pre-existing protocol for the Ventana NexES? I don't believe in
re-inventing the wheel if I don't need to.  Thanks!

 

Sally Breeden, HT(ASCP)

NM Dept. of Agriculture

Veterinary Diagnostic Services

PO Box 4700

Albuquerque, NM  87106

505-841-2576

 

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