@uams.edu>
Cc: histonet@lists.utsouthwestern.edu
Subject: AW: [Histonet] PAS/Decal Question
Hi Pam,
my personal opinion is, that 2 hours fixation is too short for sufficient
tissue-protection before acid decalcification. Formic acid at 50°C must have an
impact on glycoproteins. Wether it is a
Marcum, Pamela A via Histonet
[mailto:histonet@lists.utsouthwestern.edu]
Gesendet: Dienstag, 03. Mai 2016 18:39
An: histonet@lists.utsouthwestern.edu
Betreff: [Histonet] PAS/Decal Question
We are still having issues with our PAS stain on decaled bone marrows. The
Pathologists in HemePath are se
My impression is that your problem is during the decalcification step. It
cannot be hurried and has to be in EDTA at pH 7All reagents have to be prepared
in pH7 phosphate buffer.The inconsistency resides in the fact that not all core
Bx are the same regarding thickness, tissue condition or
We are still having issues with our PAS stain on decaled bone marrows. The
Pathologists in HemePath are seeing what they refer to as smudginess in cells
on some areas of the completed PAS slides. We have looked at everything and
cannot find where the issue is coming from at this point. We
