Xylene is becoming more and more of a nuisance material and a problem
for us in use and in disposal. We are still able to use it but with
increasing concern.
We have been able to eliminate xylene from our staining procedures
altogether in our small laboratory.
We use a hair dryer to dry
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Subject: [Histonet] Pap stain without xylene
Message-ID: <31530E35E0BAB044B3B
Hi Mike:The steps you desrcibe are wrong.After you finish staining your PAP
smear, just wash them in the last ethanol → oven dry at 60ºC for 5 minutes or
as required if the smear is too thick and when completely dried →
coverslip.This final drying has to take place at temperatures above room
René and other Histonetters,
After reading the paper:
Buesa RJ, Peshkov, MV. Histology without xylene. Ann Diagn Pathol. 2009
Aug;13(4):246-56. Epub 2009 Feb 5.
It appears that xylene in the final clearing steps is replaced with isopropanol
and mineral oil as follows:
·5:1
