What first comes in my mind is, that the tissue was a little too cold when
placed into the embedding mold. That would cause an insufficient junction
with the paraffin.
If there was not enough hot paraffin in the embedding mold and handling took
a little bit too long, or if the tissue was rather cold and dry before
putting it into the mold (cassette too long open before embedding)
something like that.
Too brittle tissue because of the processing or because it was airdried
before putting it into formalin, tends to pop out of the block. But this
would lead to obvious cutting problems.
Regards
Gudrun
-Ursprüngliche Nachricht-
Von: Mona Amin via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Gesendet: Donnerstag, 20. Juli 2023 23:36
An: histonet@lists.utsouthwestern.edu
Betreff: [Histonet] Prostate biopsy-water logged popping /lost at cutting?
Hi everyone,
We had a case of prostate core biopsies total 12 blocks
First 2 block cut fine and when cutter touched third block lost the core
completely. The blocks were on ice face down no longer than 20 mins once
faced.
The other blocks looked like they were popping out and were taken for
re-embedding and then sectioned.
Thoughts if this popping out could be because of
1- too much time in water
2- processing issue
3-condesnsation issue from cold plate
Generally too we see popping of our biopsies but with this case it was
worst.
Any help/experiences shared will be greatly appreciated as we try to
resolve the root cause.
Thank you everyone in advance.
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