[Histonet] RE: Automated Special Stainer...
Dako Artisan all the way! I've used both systems (the Artisan in my old institution). We currently have a few of the new Benchmarks on demo right now. Thus far, we're not that impressed. Frequently, there's variability on the silver stains (even in the same run,) and adjusting timing is nowhere near as flexible as on the Artisan. Even if you take away the half hour for online deparaffinization, the stains take significantly longer on the Benchmarks (than the old Nexes we are still running in our lab). Oh yeah, one more thing. The reagent tray capacity on the Artisan is about twice what it is on a Benchmark. You'll need two Benchmarks to do what one Artisan does, TATs being equal. Brian D. Cooper, HT (ASCP)CM | Histology Supervisor Department of Pathology and Laboratory Medicine Children's Hospital Los Angeles 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 bcoo...@chla.usc.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of sarah.dys...@stdavids.com Sent: Wednesday, September 17, 2014 9:13 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Automated Special Stainer... Opinions on Ventana versus Dako...Go! Sarah E. Dysart, BA, HT (ASCP), QIHC (ASCP) Pathology Supervisor St. David's North Austin Medical Center 12221 North Mopac Expressway Austin, Texas 78758 (512)901-1220 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet - CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. - ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Automated Special Stainer...
We love our Dako Artisan stainers and would not take the other brand back for any reason. The techs love the ease of use and the pathologist love the stains. What more could you ask!! Pam Marcum -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cooper, Brian Sent: Wednesday, September 17, 2014 12:33 PM To: sarah.dys...@stdavids.com; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Automated Special Stainer... Dako Artisan all the way! I've used both systems (the Artisan in my old institution). We currently have a few of the new Benchmarks on demo right now. Thus far, we're not that impressed. Frequently, there's variability on the silver stains (even in the same run,) and adjusting timing is nowhere near as flexible as on the Artisan. Even if you take away the half hour for online deparaffinization, the stains take significantly longer on the Benchmarks (than the old Nexes we are still running in our lab). Oh yeah, one more thing. The reagent tray capacity on the Artisan is about twice what it is on a Benchmark. You'll need two Benchmarks to do what one Artisan does, TATs being equal. Brian D. Cooper, HT (ASCP)CM | Histology Supervisor Department of Pathology and Laboratory Medicine Children's Hospital Los Angeles 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 bcoo...@chla.usc.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of sarah.dys...@stdavids.com Sent: Wednesday, September 17, 2014 9:13 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Automated Special Stainer... Opinions on Ventana versus Dako...Go! Sarah E. Dysart, BA, HT (ASCP), QIHC (ASCP) Pathology Supervisor St. David's North Austin Medical Center 12221 North Mopac Expressway Austin, Texas 78758 (512)901-1220 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet - CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. - ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Automated Special Stainer...
Brian wrote: ... one more thing. The reagent tray capacity on the Artisan is about twice what it is on a Benchmark. Are the TAT's equal? When we loaded up our old artisan with slides (50) and full of reagents it took over 5 hours to finish - way past our deadline. So nobody wanted to use it that way so the techs resisted and wanted to do manual to get the slide out. We ended up putting only a few stains on it. We are looking at doing specials the way we do immunos - on demand all day rather than in one batch in the AM. It seemed to me that the 20-slide units from Ventana allow small batches and run in parallel or allow staggered use. Buying a 50-slide capacity instrument and then putting only 20 slides at a time on it seems odd somehow. We looking at each of these as well and not sure yet which would serve us better. We do about 60-80 specials slides per day, and 13 stains give us 90% of our volume. Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA CONFIDENTIALITY NOTICE: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this email message or its attachments. If you believe you have received this email message in error, please contact the sender by reply email and destroy all copies of the original message. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cooper, Brian Sent: Wednesday, September 17, 2014 10:33 AM To: sarah.dys...@stdavids.com; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Automated Special Stainer... Dako Artisan all the way! I've used both systems (the Artisan in my old institution). We currently have a few of the new Benchmarks on demo right now. Thus far, we're not that impressed. Frequently, there's variability on the silver stains (even in the same run,) and adjusting timing is nowhere near as flexible as on the Artisan. Even if you take away the half hour for online deparaffinization, the stains take significantly longer on the Benchmarks (than the old Nexes we are still running in our lab). Oh yeah, one more thing. The reagent tray capacity on the Artisan is about twice what it is on a Benchmark. You'll need two Benchmarks to do what one Artisan does, TATs being equal. Brian D. Cooper, HT (ASCP)CM | Histology Supervisor Department of Pathology and Laboratory Medicine Children's Hospital Los Angeles 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 bcoo...@chla.usc.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of sarah.dys...@stdavids.com Sent: Wednesday, September 17, 2014 9:13 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Automated Special Stainer... Opinions on Ventana versus Dako...Go! Sarah E. Dysart, BA, HT (ASCP), QIHC (ASCP) Pathology Supervisor St. David's North Austin Medical Center 12221 North Mopac Expressway Austin, Texas 78758 (512)901-1220 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet - CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. - ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Automated Special Stainer...
I highly recommend the Leica Bond III. Company is excellent to work with and reagent rental a perfect solution to no capital. On line retrieval - 3 trays of 10 slides each that can be started as needed. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.we...@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy Sent: Wednesday, September 17, 2014 1:58 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Automated Special Stainer... Brian wrote: ... one more thing. The reagent tray capacity on the Artisan is about twice what it is on a Benchmark. Are the TAT's equal? When we loaded up our old artisan with slides (50) and full of reagents it took over 5 hours to finish - way past our deadline. So nobody wanted to use it that way so the techs resisted and wanted to do manual to get the slide out. We ended up putting only a few stains on it. We are looking at doing specials the way we do immunos - on demand all day rather than in one batch in the AM. It seemed to me that the 20-slide units from Ventana allow small batches and run in parallel or allow staggered use. Buying a 50-slide capacity instrument and then putting only 20 slides at a time on it seems odd somehow. We looking at each of these as well and not sure yet which would serve us better. We do about 60-80 specials slides per day, and 13 stains give us 90% of our volume. Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA CONFIDENTIALITY NOTICE: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this email message or its attachments. If you believe you have received this email message in error, please contact the sender by reply email and destroy all copies of the original message. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cooper, Brian Sent: Wednesday, September 17, 2014 10:33 AM To: sarah.dys...@stdavids.com; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Automated Special Stainer... Dako Artisan all the way! I've used both systems (the Artisan in my old institution). We currently have a few of the new Benchmarks on demo right now. Thus far, we're not that impressed. Frequently, there's variability on the silver stains (even in the same run,) and adjusting timing is nowhere near as flexible as on the Artisan. Even if you take away the half hour for online deparaffinization, the stains take significantly longer on the Benchmarks (than the old Nexes we are still running in our lab). Oh yeah, one more thing. The reagent tray capacity on the Artisan is about twice what it is on a Benchmark. You'll need two Benchmarks to do what one Artisan does, TATs being equal. Brian D. Cooper, HT (ASCP)CM | Histology Supervisor Department of Pathology and Laboratory Medicine Children's Hospital Los Angeles 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 bcoo...@chla.usc.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of sarah.dys...@stdavids.com Sent: Wednesday, September 17, 2014 9:13 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Automated Special Stainer... Opinions on Ventana versus Dako...Go! Sarah E. Dysart, BA, HT (ASCP), QIHC (ASCP) Pathology Supervisor St. David's North Austin Medical Center 12221 North Mopac Expressway Austin, Texas 78758 (512)901-1220 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet - CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies
Re: [Histonet] RE: Automated Special Stainer...
I understand the problem. We have two Artisans and rarely have a time issue. - Original Message - From: Timothy Morken timothy.mor...@ucsfmedctr.org To: Histonet histonet@lists.utsouthwestern.edu Sent: Wednesday, September 17, 2014 12:58:02 PM Subject: [Histonet] RE: Automated Special Stainer... Brian wrote: ... one more thing. The reagent tray capacity on the Artisan is about twice what it is on a Benchmark. Are the TAT's equal? When we loaded up our old artisan with slides (50) and full of reagents it took over 5 hours to finish - way past our deadline. So nobody wanted to use it that way so the techs resisted and wanted to do manual to get the slide out. We ended up putting only a few stains on it. We are looking at doing specials the way we do immunos - on demand all day rather than in one batch in the AM. It seemed to me that the 20-slide units from Ventana allow small batches and run in parallel or allow staggered use. Buying a 50-slide capacity instrument and then putting only 20 slides at a time on it seems odd somehow. We looking at each of these as well and not sure yet which would serve us better. We do about 60-80 specials slides per day, and 13 stains give us 90% of our volume. Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA CONFIDENTIALITY NOTICE: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this email message or its attachments. If you believe you have received this email message in error, please contact the sender by reply email and destroy all copies of the original message. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cooper, Brian Sent: Wednesday, September 17, 2014 10:33 AM To: sarah.dys...@stdavids.com; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Automated Special Stainer... Dako Artisan all the way! I've used both systems (the Artisan in my old institution). We currently have a few of the new Benchmarks on demo right now. Thus far, we're not that impressed. Frequently, there's variability on the silver stains (even in the same run,) and adjusting timing is nowhere near as flexible as on the Artisan. Even if you take away the half hour for online deparaffinization, the stains take significantly longer on the Benchmarks (than the old Nexes we are still running in our lab). Oh yeah, one more thing. The reagent tray capacity on the Artisan is about twice what it is on a Benchmark. You'll need two Benchmarks to do what one Artisan does, TATs being equal. Brian D. Cooper, HT (ASCP)CM | Histology Supervisor Department of Pathology and Laboratory Medicine Children's Hospital Los Angeles 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 bcoo...@chla.usc.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of sarah.dys...@stdavids.com Sent: Wednesday, September 17, 2014 9:13 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Automated Special Stainer... Opinions on Ventana versus Dako...Go! Sarah E. Dysart, BA, HT (ASCP), QIHC (ASCP) Pathology Supervisor St. David's North Austin Medical Center 12221 North Mopac Expressway Austin, Texas 78758 (512)901-1220 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet - CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. - ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Automated Special Stainer...
We've had VMS instruments from day 1 and currently have 4 Ultras. The random access capability is awesome and we can even get out EBER ISH slides the same day if put on in the morning. Our HER2 dual ISH stains run overnight as they are 14 hour protocols. Reproducibility among the 4 instruments and concurrent/consecutive stains on the same instrument are a given. Technical advice and service are outstanding. And, no, I do not have a stake in the company just am really sold by their products. Linda A. Sebree From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] on behalf of Morken, Timothy [timothy.mor...@ucsfmedctr.org] Sent: Wednesday, September 17, 2014 12:58 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Automated Special Stainer... Brian wrote: ... one more thing. The reagent tray capacity on the Artisan is about twice what it is on a Benchmark. Are the TAT's equal? When we loaded up our old artisan with slides (50) and full of reagents it took over 5 hours to finish - way past our deadline. So nobody wanted to use it that way so the techs resisted and wanted to do manual to get the slide out. We ended up putting only a few stains on it. We are looking at doing specials the way we do immunos - on demand all day rather than in one batch in the AM. It seemed to me that the 20-slide units from Ventana allow small batches and run in parallel or allow staggered use. Buying a 50-slide capacity instrument and then putting only 20 slides at a time on it seems odd somehow. We looking at each of these as well and not sure yet which would serve us better. We do about 60-80 specials slides per day, and 13 stains give us 90% of our volume. Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA CONFIDENTIALITY NOTICE: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this email message or its attachments. If you believe you have received this email message in error, please contact the sender by reply email and destroy all copies of the original message. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cooper, Brian Sent: Wednesday, September 17, 2014 10:33 AM To: sarah.dys...@stdavids.com; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Automated Special Stainer... Dako Artisan all the way! I've used both systems (the Artisan in my old institution). We currently have a few of the new Benchmarks on demo right now. Thus far, we're not that impressed. Frequently, there's variability on the silver stains (even in the same run,) and adjusting timing is nowhere near as flexible as on the Artisan. Even if you take away the half hour for online deparaffinization, the stains take significantly longer on the Benchmarks (than the old Nexes we are still running in our lab). Oh yeah, one more thing. The reagent tray capacity on the Artisan is about twice what it is on a Benchmark. You'll need two Benchmarks to do what one Artisan does, TATs being equal. Brian D. Cooper, HT (ASCP)CM | Histology Supervisor Department of Pathology and Laboratory Medicine Children's Hospital Los Angeles 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 bcoo...@chla.usc.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of sarah.dys...@stdavids.com Sent: Wednesday, September 17, 2014 9:13 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Automated Special Stainer... Opinions on Ventana versus Dako...Go! Sarah E. Dysart, BA, HT (ASCP), QIHC (ASCP) Pathology Supervisor St. David's North Austin Medical Center 12221 North Mopac Expressway Austin, Texas 78758 (512)901-1220 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet - CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. - ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman
[Histonet] RE: Automated Special Stainer...
I guess TAT's being equal is a relative term, depending upon your workload. For our purposes, it's not slide capacity that poses a problem--it's reagent capacity. We've never loaded 50 special stains at any one time--we typically don't even have half that amount in one day! So here's a scenario common to the workload at our institution. For our liver panels, we run Iron, Trichrome, Retic, PAS with and without Digestion. That pretty much maxes out the reagent capacity on the Benchmark. Sure, we can load up to 20 of these onto the Benchmark. But as is so frequently the case, we also have just 1 GMS that also needs to be stained! This is where the second Benchmark comes into play, or we'll have to stain by hand. One Artisan can handle this at the same time, and if memory serves (disclosure--it's been about 2 years, and the model I used didn't have online deparaffinization), it didn't add all that much time to the process. It was certainly faster than waiting for the machine to complete, and start another run. For our institution, it's either going to be 2 Artisans or 2 Benchmarks. The Artisans will give us some greater flexibility due to the greater reagent capacity. Brian -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy Sent: Wednesday, September 17, 2014 10:58 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Automated Special Stainer... Brian wrote: ... one more thing. The reagent tray capacity on the Artisan is about twice what it is on a Benchmark. Are the TAT's equal? When we loaded up our old artisan with slides (50) and full of reagents it took over 5 hours to finish - way past our deadline. So nobody wanted to use it that way so the techs resisted and wanted to do manual to get the slide out. We ended up putting only a few stains on it. We are looking at doing specials the way we do immunos - on demand all day rather than in one batch in the AM. It seemed to me that the 20-slide units from Ventana allow small batches and run in parallel or allow staggered use. Buying a 50-slide capacity instrument and then putting only 20 slides at a time on it seems odd somehow. We looking at each of these as well and not sure yet which would serve us better. We do about 60-80 specials slides per day, and 13 stains give us 90% of our volume. Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA CONFIDENTIALITY NOTICE: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this email message or its attachments. If you believe you have received this email message in error, please contact the sender by reply email and destroy all copies of the original message. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cooper, Brian Sent: Wednesday, September 17, 2014 10:33 AM To: sarah.dys...@stdavids.com; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Automated Special Stainer... Dako Artisan all the way! I've used both systems (the Artisan in my old institution). We currently have a few of the new Benchmarks on demo right now. Thus far, we're not that impressed. Frequently, there's variability on the silver stains (even in the same run,) and adjusting timing is nowhere near as flexible as on the Artisan. Even if you take away the half hour for online deparaffinization, the stains take significantly longer on the Benchmarks (than the old Nexes we are still running in our lab). Oh yeah, one more thing. The reagent tray capacity on the Artisan is about twice what it is on a Benchmark. You'll need two Benchmarks to do what one Artisan does, TATs being equal. Brian D. Cooper, HT (ASCP)CM | Histology Supervisor Department of Pathology and Laboratory Medicine Children's Hospital Los Angeles 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 bcoo...@chla.usc.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of sarah.dys...@stdavids.com Sent: Wednesday, September 17, 2014 9:13 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Automated Special Stainer... Opinions on Ventana versus Dako...Go! Sarah E. Dysart, BA, HT (ASCP), QIHC (ASCP) Pathology Supervisor St. David's North Austin Medical Center 12221 North Mopac Expressway Austin, Texas 78758 (512)901-1220 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Automated Special Stainer...
Sorry, I thought we were talking IHC/ISH not SS. Linda A. Sebree From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] on behalf of Cooper, Brian [bcoo...@chla.usc.edu] Sent: Wednesday, September 17, 2014 2:36 PM To: Morken, Timothy; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Automated Special Stainer... I guess TAT's being equal is a relative term, depending upon your workload. For our purposes, it's not slide capacity that poses a problem--it's reagent capacity. We've never loaded 50 special stains at any one time--we typically don't even have half that amount in one day! So here's a scenario common to the workload at our institution. For our liver panels, we run Iron, Trichrome, Retic, PAS with and without Digestion. That pretty much maxes out the reagent capacity on the Benchmark. Sure, we can load up to 20 of these onto the Benchmark. But as is so frequently the case, we also have just 1 GMS that also needs to be stained! This is where the second Benchmark comes into play, or we'll have to stain by hand. One Artisan can handle this at the same time, and if memory serves (disclosure--it's been about 2 years, and the model I used didn't have online deparaffinization), it didn't add all that much time to the process. It was certainly faster than waiting for the machine to complete, and start another run. For our institution, it's either going to be 2 Artisans or 2 Benchmarks. The Artisans will give us some greater flexibility due to the greater reagent capacity. Brian -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy Sent: Wednesday, September 17, 2014 10:58 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Automated Special Stainer... Brian wrote: ... one more thing. The reagent tray capacity on the Artisan is about twice what it is on a Benchmark. Are the TAT's equal? When we loaded up our old artisan with slides (50) and full of reagents it took over 5 hours to finish - way past our deadline. So nobody wanted to use it that way so the techs resisted and wanted to do manual to get the slide out. We ended up putting only a few stains on it. We are looking at doing specials the way we do immunos - on demand all day rather than in one batch in the AM. It seemed to me that the 20-slide units from Ventana allow small batches and run in parallel or allow staggered use. Buying a 50-slide capacity instrument and then putting only 20 slides at a time on it seems odd somehow. We looking at each of these as well and not sure yet which would serve us better. We do about 60-80 specials slides per day, and 13 stains give us 90% of our volume. Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA CONFIDENTIALITY NOTICE: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this email message or its attachments. If you believe you have received this email message in error, please contact the sender by reply email and destroy all copies of the original message. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cooper, Brian Sent: Wednesday, September 17, 2014 10:33 AM To: sarah.dys...@stdavids.com; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Automated Special Stainer... Dako Artisan all the way! I've used both systems (the Artisan in my old institution). We currently have a few of the new Benchmarks on demo right now. Thus far, we're not that impressed. Frequently, there's variability on the silver stains (even in the same run,) and adjusting timing is nowhere near as flexible as on the Artisan. Even if you take away the half hour for online deparaffinization, the stains take significantly longer on the Benchmarks (than the old Nexes we are still running in our lab). Oh yeah, one more thing. The reagent tray capacity on the Artisan is about twice what it is on a Benchmark. You'll need two Benchmarks to do what one Artisan does, TATs being equal. Brian D. Cooper, HT (ASCP)CM | Histology Supervisor Department of Pathology and Laboratory Medicine Children's Hospital Los Angeles 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 bcoo...@chla.usc.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of sarah.dys...@stdavids.com Sent: Wednesday, September 17, 2014 9:13 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Automated Special Stainer
