Re: [Histonet] Staining issues

2016-12-28 Thread Tony Henwood (SCHN) via Histonet
] Staining issues Our pathologists are complaining that the center of tissues are not staining properly in both our H's and IHC's. Can anyone provide some thoughts as to where this problem could be occurring? As a separate situation we are experiencing the tissues folding or falling off during

Re: [Histonet] Staining issues

2016-12-28 Thread Rene J Buesa via Histonet
Improper staining at the center and falling sections are typical consequences of poor fixation/infiltration.If you have changed nothing proceduraly, what about somebody "new" grossing and preparing thicker tissue slices?René On Wednesday, December 28, 2016 8:34 AM, Charles Riley via

[Histonet] Staining issues

2016-12-28 Thread Charles Riley via Histonet
Our pathologists are complaining that the center of tissues are not staining properly in both our H's and IHC's. Can anyone provide some thoughts as to where this problem could be occurring? As a separate situation we are experiencing the tissues folding or falling off during the staining