Re: [Histonet] Suggestions for making glutaraldehyde penetrate in between little setae of insects for microscopy

2016-02-26 Thread Jay Lundgren via Histonet 
A little alcohol will lower the surface tension of aqueous solutions.  Then
there's always DMSO.


   Sincerely,

Jay A. Lundgren, M.S., HTL (ASCP)

On Fri, Feb 26, 2016 at 8:15 AM, Jorge A. Santiago-Blay via Histonet <
histonet@lists.utsouthwestern.edu> wrote:

> Hello Histonetetrs:
>
> I am trying to fix insect wings for TEM. Less than ideal fixation appears
> to be caused by abundant setae on the surface of the wings. Those setae,
> trap air, thus making penetration of the fixative more difficult difficult.
> Interestingly, also the wings tend to curl upon themselves.
>
> Options re. fixation:
> 1. Just submerging the insects mechanically does not seem to solve the
> problem.
>
> 2. We are contemplating submerging and evacuating (intermittently) a few
> times for 2 minutes (vacuuming)
>
> 3. Are there chemicals that can break the bubbles formed in between the
> setae?
>
> 4. Any other comments or suggestions?
>
> Options to minimize (ideally eliminate) curling of the wings?
>
> If you have any constructive suggestions, please kindly email it to me
> directly.
>
> blayjo...@gmail.com
>
> Of course, we intend to begin with new freshly collected insects.
>
> Gratefully,
>
> Jorge
>
> Jorge A. Santiago-Blay, PhD
> blaypublishers.com
>
> 1. Positive experiences for authors of papers published in *LEB*
> http://blaypublishers.com/testimonials/
>
> 2. Free examples of papers published in *LEB*:
> http://blaypublishers.com/category/previous-issues/.
>
> 3. *Guidelines for Authors* and page charges of *LEB*:
> http://blaypublishers.com/archives/ *.*
>
> 4. Want to subscribe to *LEB*? http://blaypublishers.com/subscriptions/
>
>
> http://blayjorge.wordpress.com/
> http://paleobiology.si.edu/staff/individuals/santiagoblay.cfm
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Re: [Histonet] Suggestions for making glutaraldehyde penetrate in between little setae of insects for microscopy

2016-02-26 Thread Simmons, Christopher via Histonet 
Double PPE if you use DMSO


Sent from my iPhone

> On Feb 26, 2016, at 2:41 PM, Jay Lundgren via Histonet 
>  wrote:
> 
> A little alcohol will lower the surface tension of aqueous solutions.  Then
> there's always DMSO.
> 
> 
>   Sincerely,
> 
>Jay A. Lundgren, M.S., HTL (ASCP)
> 
> On Fri, Feb 26, 2016 at 8:15 AM, Jorge A. Santiago-Blay via Histonet <
> histonet@lists.utsouthwestern.edu> wrote:
> 
>> Hello Histonetetrs:
>> 
>> I am trying to fix insect wings for TEM. Less than ideal fixation appears
>> to be caused by abundant setae on the surface of the wings. Those setae,
>> trap air, thus making penetration of the fixative more difficult difficult.
>> Interestingly, also the wings tend to curl upon themselves.
>> 
>> Options re. fixation:
>> 1. Just submerging the insects mechanically does not seem to solve the
>> problem.
>> 
>> 2. We are contemplating submerging and evacuating (intermittently) a few
>> times for 2 minutes (vacuuming)
>> 
>> 3. Are there chemicals that can break the bubbles formed in between the
>> setae?
>> 
>> 4. Any other comments or suggestions?
>> 
>> Options to minimize (ideally eliminate) curling of the wings?
>> 
>> If you have any constructive suggestions, please kindly email it to me
>> directly.
>> 
>> blayjo...@gmail.com
>> 
>> Of course, we intend to begin with new freshly collected insects.
>> 
>> Gratefully,
>> 
>> Jorge
>> 
>> Jorge A. Santiago-Blay, PhD
>> blaypublishers.com
>> 
>> 1. Positive experiences for authors of papers published in *LEB*
>> http://blaypublishers.com/testimonials/
>> 
>> 2. Free examples of papers published in *LEB*:
>> http://blaypublishers.com/category/previous-issues/.
>> 
>> 3. *Guidelines for Authors* and page charges of *LEB*:
>> http://blaypublishers.com/archives/ *.*
>> 
>> 4. Want to subscribe to *LEB*? http://blaypublishers.com/subscriptions/
>> 
>> 
>> http://blayjorge.wordpress.com/
>> http://paleobiology.si.edu/staff/individuals/santiagoblay.cfm
>> ___
>> Histonet mailing list
>> Histonet@lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet

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[Histonet] Suggestions for making glutaraldehyde penetrate in between little setae of insects for microscopy

2016-02-26 Thread Jorge A. Santiago-Blay via Histonet 
Hello Histonetetrs:

I am trying to fix insect wings for TEM. Less than ideal fixation appears
to be caused by abundant setae on the surface of the wings. Those setae,
trap air, thus making penetration of the fixative more difficult difficult.
Interestingly, also the wings tend to curl upon themselves.

Options re. fixation:
1. Just submerging the insects mechanically does not seem to solve the
problem.

2. We are contemplating submerging and evacuating (intermittently) a few
times for 2 minutes (vacuuming)

3. Are there chemicals that can break the bubbles formed in between the
setae?

4. Any other comments or suggestions?

Options to minimize (ideally eliminate) curling of the wings?

If you have any constructive suggestions, please kindly email it to me
directly.

blayjo...@gmail.com

Of course, we intend to begin with new freshly collected insects.

Gratefully,

Jorge

Jorge A. Santiago-Blay, PhD
blaypublishers.com

1. Positive experiences for authors of papers published in *LEB*
http://blaypublishers.com/testimonials/

2. Free examples of papers published in *LEB*:
http://blaypublishers.com/category/previous-issues/.

3. *Guidelines for Authors* and page charges of *LEB*:
http://blaypublishers.com/archives/ *.*

4. Want to subscribe to *LEB*? http://blaypublishers.com/subscriptions/


http://blayjorge.wordpress.com/
http://paleobiology.si.edu/staff/individuals/santiagoblay.cfm
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