Re: [Histonet] Tissue processor validation
Get your gross lab to provide you with samples representative of what you normally process and the same for tissue you use for internally procured special stain controls (kidney, lung, etc) Process as usual and cut and perform specials If they work: done. Get Outlook for iOS<https://aka.ms/o0ukef> From: Martha Ward-Pathology via Histonet Sent: Wednesday, September 2, 2020 3:51:42 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Tissue processor validation I am posting this question for my colleague in the Histology lab. She is working on a plan for a new tissue processor validation, including special stains, and would like to get input from anyone that has already gone through it.This is the first time anyone here has ever been involved in validating a new processor. Current ones are 25+ years. Any help or advice would be appreciated. Thanks in advance! Martha Ward, MT (ASCP) QIHC Wake Forest Baptist Health ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Tissue processor validation
I am posting this question for my colleague in the Histology lab. She is working on a plan for a new tissue processor validation, including special stains, and would like to get input from anyone that has already gone through it.This is the first time anyone here has ever been involved in validating a new processor. Current ones are 25+ years. Any help or advice would be appreciated. Thanks in advance! Martha Ward, MT (ASCP) QIHC Wake Forest Baptist Health ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Tissue Processor Validation
Hello Everyone, If we change a processing protocol and are in need to recalibrate our tissue processors, is it required to perform concordance study between the old protocol and the new? Please let me know Thanks Sent from my iPhone ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Tissue Processor Validation Procedure
Hi Allison I hope the attached can help you Best Regards, Jamal M. Al Rowaihi Anatomic Pathology Supervisor | Al Borg Medical Laboratories | Mobile +966 503629832| j.rowa...@alborglaboratories.com Palestine St, Al Rajhi Building, P.O. Box 52817, Jeddah 21573, KSA| Phone: +966 12 670 0099 | Fax: +966 12 676 4984 | www.alborglaboratories.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Scott, Allison D Sent: Thursday, January 29, 2015 10:44 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Tissue Processor Validation Procedure Hello to all in Histoland. Does anyone have a tissue processor validation procedure that they would be willing to share? Any help in this would be greatly appreciated. Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital 713-566-5287(Lab) 713-566-2148(Office) CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 (HIPAA), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Tissue Processor Validation Procedure
Hello Allison, When we purchased a new processor, I chose tissue specimens representative of what we normally see in our lab (surgical/biopsy and necropsy tissues) from cases which had been signed out within the previous 48-72 hours. I then ran parallel samples on both processors (same protocols, run the same day, fresh reagents in both processors) and then set up a grading scale for the pathologists to do a blind comparison. After that, I did a (brief) statistical analysis to determine if there was any significant difference between slides from the two processors. For IHC (we are a small lab with a short IHC menu currently), I ran parallel Vimentin and Multicytokeratin on the same blocks as used for the HE and did a comparison just as for the HE slides. I presented the final data to my chief pathologist who then signed off on the validation. I keep a copy of the validation with my equipment records. I hope this helps. Best regards, Carole Carole Johnson Carole Johnson, HT(ASCP)cm New Mexico Department of Agriculture Veterinary Diagnostic Services 505.383.9299 To understand is to stand under, which is to look up, which is a good way to understand Confidentiality Notice: New Mexico has a very broad public records law. Most written communications to or from state employees are public records. Your e-mail communications may therefore be subject to public disclosure. This e-mail, including all attachments is for the sole use of the intended recipients. Any unauthorized review, use, disclosure or distribution is prohibited unless specifically provided under the New Mexico Inspection of Public Records Act. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Tissue Processor Validation Procedure
Hello to all in Histoland. Does anyone have a tissue processor validation procedure that they would be willing to share? Any help in this would be greatly appreciated. Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital 713-566-5287(Lab) 713-566-2148(Office) CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 (HIPAA), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Tissue Processor Validation Procedure
Dear colleague I hope the attached help you Best Regards, Jamal M. Al Rowaihi Anatomic Pathology Supervisor | Al Borg Medical Laboratories | Mobile +966 503629832| j.rowa...@alborglaboratories.com Palestine St, Al Rajhi Building, P.O. Box 52817, Jeddah 21573, KSA| Phone: +966 12 670 0099 | Fax: +966 12 676 4984 | www.alborglaboratories.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Adesupo, Adesuyi (Banjo) Sent: Thursday, November 06, 2014 11:49 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Tissue Processor Validation Procedure Hi, I am wondering if you guys in histoland will like to share your Tissue Processor Validation Procedure with me. Thanks, Adesuyi == CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution, or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender immediately and destroy all copies of this communication and any attachments. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Tissue Processor Validation Procedure
Hi, I am wondering if you guys in histoland will like to share your Tissue Processor Validation Procedure with me. Thanks, Adesuyi == CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution, or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender immediately and destroy all copies of this communication and any attachments. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Tissue Processor Validation:
Hi Jb The attached is my validation form you can refer to it. Best Regards, Jamal M. Al Rowaihi Anatomic Pathology Supervisor | Al Borg Medical Laboratories | Mobile +966 503629832| j.rowa...@alborglaboratories.com Palestine St, Al Rajhi Building, P.O. Box 52817, Jeddah 21573, KSA| Phone: +966 12 670 0099 | Fax: +966 12 676 4984 | www.alborglaboratories.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jb Sent: Friday, September 05, 2014 9:11 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Tissue Processor Validation: Does anyone have a tissue processor validation form that they are willing to share? Thank you. Sent from my iPhone ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Tissue Processor Validation:
It appears that the list software strips attachments. You may want to put it on a website and post the URL. Mike Andrews, W5EGO WWME Oklahoma area executive team On Sep 6, 2014, at 4:34 AM, Jamal j.rowa...@alborglaboratories.com wrote: Hi Jb The attached is my validation form you can refer to it. Best Regards, Jamal M. Al RowaihiAnatomic Pathology Supervisor | Al Borg Medical Laboratories | Mobile +966 503629832| j.rowa...@alborglaboratories.com Palestine St, Al Rajhi Building, P.O. Box 52817, Jeddah 21573, KSA| Phone: +966 12 670 0099 | Fax: +966 12 676 4984 | www.alborglaboratories.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jb Sent: Friday, September 05, 2014 9:11 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Tissue Processor Validation: Does anyone have a tissue processor validation form that they are willing to share? Thank you. Sent from my iPhone ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Tissue Processor Validation:
Does anyone have a tissue processor validation form that they are willing to share? Thank you. Sent from my iPhone ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Tissue Processor Validation
Dear All Would any one of you out there,want to share template of Tissue Processor validation forms? Thanks Muhammad Tahseen Histolog Supervisor SKMCHRC Lahore Pakistan On 2013-08-28 19:10, Horn, Hazel V wrote: We did the same as Beth. Hazel Horn ySupervisor of Histolog/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1241 fax hor...@archildrens.org archildrens.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Beth Brinegar Sent: Wednesday, August 28, 2013 8:40 AM To: Rathborne, Toni Cc: histonet@lists.utsouthwestern.edu; dianar...@aol.com Subject: Re: [Histonet] Tissue Processor Validation Yes, we do a few validation runs. Nothing too extreme, however we gross some extra patient tissue and do a small run, then increase the amount of blocks processed until we are ready to run cases for patient diagnosis. It really only takes a week or two. After those blocks are cut, then we compare them to the previous processor results and make changes if neccessary. Beth Brinegar HTL(ASCP) Anatomic Pathology Supervisor Mercy Medical Center Cedar Rapids, IA 52403 On Wed, Aug 28, 2013 at 8:27 AM, Rathborne, Toni trathbo...@somerset-healthcare.com wrote: How do you validate IHC FISH with a new processor? -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto: histonet-boun...@lists.utsouthwestern.edu] On Behalf Of joelle weaver Sent: Wednesday, August 28, 2013 9:23 AM To: dianar...@aol.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Tissue Processor Validation Yes definitely, 5 programs, 25-50 sister, serial sections on tissue that represents patient samples. Joelle Weaver MAOM, HTL (ASCP) QIHC From: dianar...@aol.com Date: Tue, 27 Aug 2013 22:07:19 -0400 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Tissue Processor Validation I am interested in knowing how many of you out there Validate your Tissue Processors before you put them into use and how extreme you go. Diana Ripley ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Tissue Processor Validation
Yes, we do a few validation runs. Nothing too extreme, however we gross some extra patient tissue and do a small run, then increase the amount of blocks processed until we are ready to run cases for patient diagnosis. It really only takes a week or two. After those blocks are cut, then we compare them to the previous processor results and make changes if neccessary. Beth Brinegar HTL(ASCP) Anatomic Pathology Supervisor Mercy Medical Center Cedar Rapids, IA 52403 On Wed, Aug 28, 2013 at 8:27 AM, Rathborne, Toni trathbo...@somerset-healthcare.com wrote: How do you validate IHC FISH with a new processor? -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto: histonet-boun...@lists.utsouthwestern.edu] On Behalf Of joelle weaver Sent: Wednesday, August 28, 2013 9:23 AM To: dianar...@aol.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Tissue Processor Validation Yes definitely, 5 programs, 25-50 sister, serial sections on tissue that represents patient samples. Joelle Weaver MAOM, HTL (ASCP) QIHC From: dianar...@aol.com Date: Tue, 27 Aug 2013 22:07:19 -0400 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Tissue Processor Validation I am interested in knowing how many of you out there Validate your Tissue Processors before you put them into use and how extreme you go. Diana Ripley ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Tissue Processor Validation
On Wed, Aug 28, 2013 at 8:27 AM, Rathborne, Toni trathbo...@somerset-healthcare.com wrote: How do you validate IHC FISH with a new processor? You are going to have to revalidate IHC and FISH with new processing. How much might depend on if you are significantly changing instrumentation/technology, or just getting a new instrument of the same type. I usually don't do a full, as new assay validation, but the amount needed just to show that the processing change has not affected the test results. If there are issues that appear with that, you might have to do a complete revalidation. Joelle Weaver MAOM, HTL (ASCP) QIHC From: hor...@archildrens.org To: bbrinegar...@gmail.com; trathbo...@somerset-healthcare.com Date: Wed, 28 Aug 2013 09:10:39 -0500 Subject: RE: [Histonet] Tissue Processor Validation CC: histonet@lists.utsouthwestern.edu; dianar...@aol.com We did the same as Beth. Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1241 fax hor...@archildrens.org archildrens.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Beth Brinegar Sent: Wednesday, August 28, 2013 8:40 AM To: Rathborne, Toni Cc: histonet@lists.utsouthwestern.edu; dianar...@aol.com Subject: Re: [Histonet] Tissue Processor Validation Yes, we do a few validation runs. Nothing too extreme, however we gross some extra patient tissue and do a small run, then increase the amount of blocks processed until we are ready to run cases for patient diagnosis. It really only takes a week or two. After those blocks are cut, then we compare them to the previous processor results and make changes if neccessary. Beth Brinegar HTL(ASCP) Anatomic Pathology Supervisor Mercy Medical Center Cedar Rapids, IA 52403 On Wed, Aug 28, 2013 at 8:27 AM, Rathborne, Toni trathbo...@somerset-healthcare.com wrote: How do you validate IHC FISH with a new processor? -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto: histonet-boun...@lists.utsouthwestern.edu] On Behalf Of joelle weaver Sent: Wednesday, August 28, 2013 9:23 AM To: dianar...@aol.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Tissue Processor Validation Yes definitely, 5 programs, 25-50 sister, serial sections on tissue that represents patient samples. Joelle Weaver MAOM, HTL (ASCP) QIHC From: dianar...@aol.com Date: Tue, 27 Aug 2013 22:07:19 -0400 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Tissue Processor Validation I am interested in knowing how many of you out there Validate your Tissue Processors before you put them into use and how extreme you go. Diana Ripley ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Tissue Processor Validation
I am interested in knowing how many of you out there Validate your Tissue Processors before you put them into use and how extreme you go. Diana Ripley ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
