Re: [Histonet] Vacuolated and torn?
If it is a block issue you can also often see 'white' bits on the block surface that correlate to the torn areas on the slide, try 'polishing' the block at 1um until they go away, cool and then try sectioning again. I am unsure how that relates to vacuolation - as I have usually seen that referred to an intracellular event - and not to refer to a regular hole. I also suggest having someone else cut a block and then compare. yours, mills On Thu, Oct 6, 2016 at 3:43 PM, Cartun, Richard via Histonet < histonet@lists.utsouthwestern.edu> wrote: > Microtomy is so important, but often over-looked. You might try giving > the paraffin block(s) to different people and have them all cut H, and > then have your pathologist compare them. If everyone's sections are > suboptimal, then the problem is with fixation and/or processing. > > Richard > > Richard W. Cartun, MS, PhD > Director, Histology & The Martin M. Berman, MD Immunopathology & > Morphologic Proteomics Laboratory > Director, Biospecimen Collection Programs > Assistant Director, Anatomic Pathology > Hartford Hospital > 80 Seymour Street > Hartford, CT 06102 > (860) 972-1596 > (860) 545-2204 Fax > > > > -Original Message- > From: Martin, Erin via Histonet [mailto:histonet@lists.utsouthwestern.edu] > Sent: Thursday, October 06, 2016 9:31 AM > To: histonet > Subject: [Histonet] Vacuolated and torn? > > Good morning everyone! > > One of my pathologists says that we are having a problem with the tissue > on the slides looking vacuolated and torn. He is convinced it is from > microtomy. Anyone have any ideas? I was thinking that it might be a > processing issue. > > > > Thanks in advance! > > Erin Martin, Histology Supervisor > UCSF Dermatopathology and Oral Pathology Service > 1701 Divisadero St, San Francisco, CA 94044 > 415-353-7248 > > Confidentiality Notice > The information transmitted is intended only for the person or entity to > which it is addressed and may contain confidential and/or privileged > material. Any review, retransmission, dissemination or other use of, or > taking of any action in reliance upon, this information by persons or > entities other than the intended recipient is prohibited. If you receive > this in error please contact the sender and delete the material from any > computer. > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > This e-mail message, including any attachments, is for the sole use of the > intended recipient(s) and may contain confidential and privileged > information. Any unauthorized review, use, disclosure, or distribution is > prohibited. If you are not the intended recipient, or an employee or agent > responsible for delivering the message to the intended recipient, please > contact the sender by reply e-mail and destroy all copies of the original > message, including any attachments. > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Caroline Miller (mills) Director of Histology 3Scan.com 415 2187297 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Vacuolated and torn?
Microtomy is so important, but often over-looked. You might try giving the paraffin block(s) to different people and have them all cut H, and then have your pathologist compare them. If everyone's sections are suboptimal, then the problem is with fixation and/or processing. Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (860) 545-2204 Fax -Original Message- From: Martin, Erin via Histonet [mailto:histonet@lists.utsouthwestern.edu] Sent: Thursday, October 06, 2016 9:31 AM To: histonet Subject: [Histonet] Vacuolated and torn? Good morning everyone! One of my pathologists says that we are having a problem with the tissue on the slides looking vacuolated and torn. He is convinced it is from microtomy. Anyone have any ideas? I was thinking that it might be a processing issue. Thanks in advance! Erin Martin, Histology Supervisor UCSF Dermatopathology and Oral Pathology Service 1701 Divisadero St, San Francisco, CA 94044 415-353-7248 Confidentiality Notice The information transmitted is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by persons or entities other than the intended recipient is prohibited. If you receive this in error please contact the sender and delete the material from any computer. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Vacuolated and torn?
Good morning everyone! One of my pathologists says that we are having a problem with the tissue on the slides looking vacuolated and torn. He is convinced it is from microtomy. Anyone have any ideas? I was thinking that it might be a processing issue. Thanks in advance! Erin Martin, Histology Supervisor UCSF Dermatopathology and Oral Pathology Service 1701 Divisadero St, San Francisco, CA 94044 415-353-7248 Confidentiality Notice The information transmitted is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by persons or entities other than the intended recipient is prohibited. If you receive this in error please contact the sender and delete the material from any computer. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
