tern.edu>
To: histonet@lists.utsouthwestern.edu
Sent: Thursday, March 23, 2017 6:28:24 AM
Subject: [Histonet] low signal for long post fixation
Dear all,
I have mouse brain tissue perfused with PFA 4% and post fixed in formol 10%
for periods between 2 month and 1 year. I cut them on vi
Perhaps your only solution is to increase HIARRené
On Thursday, March 23, 2017 9:39 AM, Mariela Chertoff via Histonet
wrote:
Dear all,
I have mouse brain tissue perfused with PFA 4% and post fixed in formol 10%
for periods between 2 month and 1 year.
Dear all,
I have mouse brain tissue perfused with PFA 4% and post fixed in formol 10%
for periods between 2 month and 1 year. I cut them on vibratome (30um) and
I made inmunohistochemistry and beta galactosidase to check senescence.
My problem is that I have less signal on positive controls if