Re: [Histonet] low signal for long post fixation

2017-03-23 Thread Ray via Histonet
tern.edu> To: histonet@lists.utsouthwestern.edu Sent: Thursday, March 23, 2017 6:28:24 AM Subject: [Histonet] low signal for long post fixation Dear all, I have mouse brain tissue perfused with PFA 4% and post fixed in formol 10% for periods between 2 month and 1 year. I cut them on vi

Re: [Histonet] low signal for long post fixation

2017-03-23 Thread Rene J Buesa via Histonet
Perhaps your only solution is to increase HIARRené On Thursday, March 23, 2017 9:39 AM, Mariela Chertoff via Histonet wrote: Dear all, I have mouse brain tissue perfused with PFA 4% and post fixed in formol 10% for periods between 2 month and 1 year.

[Histonet] low signal for long post fixation

2017-03-23 Thread Mariela Chertoff via Histonet
Dear all, I have mouse brain tissue perfused with PFA 4% and post fixed in formol 10% for periods between 2 month and 1 year. I cut them on vibratome (30um) and I made inmunohistochemistry and beta galactosidase to check senescence. My problem is that I have less signal on positive controls if