Re: [Histonet] Tissue processor advice
Hi Colleen, We have all VIPs here at BMC but I have used the Leica in the past at a previous position. We currently have 3 - E300s, 3 - VIP 5s, and 2 - VIP 6s. The VIPs are great and really only need preventive stuff like O rings and lubrication. A service company for us Avantik takes great care of all of that. You can get refurbished VIPs at great prices and they will last! Chuck Bacon, HTL(ASCP)CM Supervisor Histology Baystate Medical Center 361 Whitney Ave., Holyoke, MA 01040 Telephone: 413-322-4786 Fax: 413-322-4790 charles.ba...@baystatehealth.org -Original Message- From: Colleen Forster Sent: Wednesday, September 16, 2020 1:17 PM To: histonet-request Subject: [Histonet] Tissue processor advice HEllo Histoneters, I am looking at replacing my VIP2000, porr girl finally quit on me. The two processors I am looking at: Leica ASP300S Sakura VIP5 Any of you out there who have used either of these or both, can you give me pros , cons, yes, noI am just looking for experiences those who have used them can share. Thank you in advance. Colleen Forster HT(ASCP)QIHC BLS Histology and IHC Laboratory Jackson Hall, Room 2-155 612-626-1930 -- Please view our annual report at http://www.bhannualreport.org CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please reply to the sender immediately or by telephone at 413-794- and destroy all copies of this communication and any attachments. For further information regarding Baystate Health's privacy policy, please visit our Internet site at https://www.baystatehealth.org. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Tissue Processor Advice
We are using the Peloris with a 2 hr, 4 hr and 8 hr protocol. We run 2 hour protocols throughout the day with an average of 4-5 runs per day depending on specimen volume. We really like this processor. We have had them for 10 months now, are using factory protocols and have not had any specimens that have been either under or over processed. The techs and the pathologists are very pleased with it. Steve -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of caymanfl...@gmail.com Sent: Friday, October 22, 2010 4:17 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Tissue Processor Advice We are in need of some advice regarding rapid tissue processors. Models we are considering: Sakura Xpress Leica Peloris Thermo STP 420 It seems none of these models are perfect in every respect. I'm interested in anyone's opinions of these processors and your experience with them. All input is appreciated! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Tissue Processor Advice
How are you meeting the hours of fixation requirement for Breast? With 2 and 4 and 8 hours,, But recently there are articles calling for Her 2 to be done on GI cases. Just want to know you insight on this? -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Feher, Stephen Sent: Friday, October 22, 2010 2:12 PM To: caymanfl...@gmail.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Tissue Processor Advice We are using the Peloris with a 2 hr, 4 hr and 8 hr protocol. We run 2 hour protocols throughout the day with an average of 4-5 runs per day depending on specimen volume. We really like this processor. We have had them for 10 months now, are using factory protocols and have not had any specimens that have been either under or over processed. The techs and the pathologists are very pleased with it. Steve -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of caymanfl...@gmail.com Sent: Friday, October 22, 2010 4:17 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Tissue Processor Advice We are in need of some advice regarding rapid tissue processors. Models we are considering: Sakura Xpress Leica Peloris Thermo STP 420 It seems none of these models are perfect in every respect. I'm interested in anyone's opinions of these processors and your experience with them. All input is appreciated! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __ This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. __ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Tissue Processor Advice
There are so many good to great processors on the market, but all have their plus and minus issues. You really have to decide what your two or three most important issues will be and then rank them. With the trend in becoming more efficient/cost effective, reducing TAT and LEAN process improvement, I suggest you look to improve your process to match these trends and you will be lead to rapid tissue processing in a LEAN way. Couple the previously mentioned trends/issues with versatility of processing with your routine formalin fixed samples with molecular fixed samples on the same instrument, I suggest the Sakura Xpress (X50 or X120) rapid processors. These instruments provide continuous loading, small batch and require a small volume of reagent for processing and then discard. The instruments do have a required reagent kit and there is a variable pre-processing protocol, depending on the tissue type and fat content. Using the reagent kit does allow for cost savings over conventional processing and I find the pre-processing allows for better precision processing techniques and protocols, we have never over processed tissues. Another great advantage is the increased velocity of the workflow as the instruments are continuous load (no cleaning cycle between batches) and small batch (1 to 40 cassettes). Loading 1 or 2 cassettes when a STAT or RUSH cases arrives and completes fixation does not interrupt the process or require special handling. An important factor to consider is that continuous load processing does assist in workload leveling, which can assist in reducing employee stress, increase productivity and error reduction. All these things lead directly to reduced TAT. Add the often overlooked advantage of removing Xylene from your tissue processing, and again, I suggest you consider the Xpress. I was an early adopter (5+ yrs use) and continue to use the X120 (2 units). I have not experienced any instrument performance or maintenance issues. I have had three software upgrades and the instruments had to go down for several hours to install the upgrade. The X120 and now the X50 have two programming options 1+ hour or 2+ hour processing. The most LEAN factor is that after the first basket of up to 40 cassettes, the next one comes off 20 or 40 minutes later and you can continuously load. There is no other instrument that can allow you to process in as small as batch or provide the continuous delivery of cassettes. You can do rapid processing with all of the instruments you are considering, but conventional, one reaction chamber instruments will limit the number of times the instrument can be run each day and that increases the batch size. Rapid processing does demand change in the way your lab does it's work. The first is standardization of gross dissection to 3 mm thickness. It does not matter the instrument, if you want to truly move into rapid processing, then you have to standardize your process in the gross room. The process of retraining and standardizing your gross room is well worth the effort. Another issue that you have to fully consider is how will specific fixation times affect your workflow. We now have specific guidelines for ER/PgR; Her2 and if you follow the NCI protocols for cancer tumors, you have to record your actual fixation times. Trying to manage all the different fixation times becomes difficult and will slow down your process. If you use your tissue processor to complete fixation, the processor will force you into larger batches. When you have to wait for tissues to complete fixation before starting your processing program, you also limit the number of times the processor can be run in a day. I find that separating fixation from processing is the best approach. You process specimens only when the optimal fixation time has been completed. Stop and consider all the different tissue types, size, fat content and required protocols, and you will see the value of a rapid processor that has small batch and continuous load capabilities. Meet all your fixation requirements and needs and only use the tissue processor for processing, not fixation. This is very LEAN concept and a concept that I believe you will need to embrace. Whenever you have the opportunity to change your process, I always suggest you look to improve the process, use the latest process improvement techniques and select an instrument that will assist in the change and prepare you for future change. My philosophy is we cannot continue to do tomorrow what we do today and expect a different outcome or result. Just my thoughts and experience, I hope this will help you. Do not hesitate to contact me if you have any questions. William DeSalvo, B.S., HTL(ASCP) Date: Fri, 22 Oct 2010 16:16:50 -0400 From: caymanfl...@gmail.com To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Tissue Processor Advice We are in need of some advice regarding rapid tissue
Re: [Histonet] Tissue Processor Advice
Sakura Tissue Tek VIP5!! I have 2 of these sturdy workhorses and am very happy Have been associated with VIP5's for as long as they have been around - wonderful machines! AnnieinAbuDhabi aka.Annie out of Africa 2009/8/5 Igor Deyneko igor.deyn...@gmail.com Dear Histonetters! I need your advice in Tissue Processors. The one we currently use, Tissue Tek VIP 3000, is archaic and has finally died. So we are looking into buying a new one. I know that Thermo and Leica both have processors, as well as new Tissue teks, but I wanted to get opinions if you have a preference of a machine, pros and cons of each. I mostly process tumors, with occasional mouse organs thrown in. Any suggestions will be very helpful! Thank you in advance. Sincerely, Igor Deyneko Infinity Pharmaceuticals Cambridge, MA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Anne van Binsbergen (Hope) Abu Dhabi UAE ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Tissue Processor Advice
I recommend the new tissue tech. I use it in my lab and its reliable and intuitive. I strongly warn against TBS. I use one in my other lab and its been nothing but trouble. Its chemical storage is unreliable and leads to cross contamination. --Original Message-- From: Igor Deyneko Sender: histonet-boun...@lists.utsouthwestern.edu To: Histonet Subject: [Histonet] Tissue Processor Advice Sent: Aug 4, 2009 1:43 PM Dear Histonetters! I need your advice in Tissue Processors. The one we currently use, Tissue Tek VIP 3000, is archaic and has finally died. So we are looking into buying a new one. I know that Thermo and Leica both have processors, as well as new Tissue teks, but I wanted to get opinions if you have a preference of a machine, pros and cons of each. I mostly process tumors, with occasional mouse organs thrown in. Any suggestions will be very helpful! Thank you in advance. Sincerely, Igor Deyneko Infinity Pharmaceuticals Cambridge, MA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sent from my Verizon Wireless BlackBerry___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
