RE: [Histonet] Validation of IHC

[Pathology-Histology Sr. Supervisor]

Dear Jamal,
Please see 
Principles of Analytic Validation of Immunohistochemistry Assays
Published : Archives of Pathology and Laboratory Medicine
March 19, 2014
cap.org
Later on I will send you template of IHC Validation, which we are using in 
SKMCHRC.
Regards
Muhammad Tahseen
Senior Supervisor Histopathology
SKMCHRC
Lahore, Pakistan


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jamal
Sent: Saturday, July 19, 2014 3:42 PM
To: Histonet edu
Subject: [Histonet] Validation of IHC

Dear colleagues

We purchased Ventana GX from Rosh for IHC

I reviewed the resent CAP check list 2013 for validation of IHC but I got 
confused.

I am using ready to use prediluted antibodies from Ventana and I want to 
validate the antibodies before use according to CAP requirements.

Can I have the validation plane and procedure for IHC antibodies.

 

 

 

Best Regards,

 

 

Jamal M. Al Rowaihi  Anatomic Pathology Supervisor   | Al Borg
Medical Laboratories |  Mobile +966 503629832| 
mailto:j.rowa...@alborglaboratories.com j.rowa...@alborglaboratories.com 

Palestine St, Al Rajhi Building, P.O. Box 52817, Jeddah 21573, KSA|
Phone: +966 12 670 0099   | Fax: +966 12 676 4984 |
http://www.alborglaboratories.com/ www.alborglaboratories.com

 

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Re: [Histonet] Validation of IHC

[Rene J Buesa]

If you go to HistoNet archives you will find detailed answers to your questions 
posted about 2 months ago.
Every time you change anything in any protocol, that change has to be validated.
René J.

--- On Wed, 10/14/09, Jason McGough jmcgo...@clinlab.com wrote:


From: Jason McGough jmcgo...@clinlab.com
Subject: [Histonet] Validation of IHC
To: histonet@lists.utsouthwestern.edu
Date: Wednesday, October 14, 2009, 5:51 PM


Our lab is inquiring about how other labs are validating their IHC stains. We 
currently are processing specimens both in a microwave and conventional 
processors. Are labs validating every type of program on the conventional and 
microwave processors? (i.e. small biopsies vs. larger tissue samples)Or just 
microwave vs. conventional processing? Also how many blocks of each tissue 
control are you testing? Thanks in advance for your replies.


Jason McGough HT(ASCP)
Account Representative-Anatomic Pathology
Clinical Laboratory of the Black Hills
2805 5th Street Suite 210
Rapid City, SD 57701
605-343-2267
jmcgo...@clinlab.com



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