You could always get formical which fixes and decals at the same time. This
would eliminate the waiting for fixation. Although I do usually let it fix in
straight formalin overnight first.
Sarah Goebel, BA, HT(ASCP)
Histotechnologist
Mirna Therapeutics
2150 Woodward Street
Suite 100
Austin, Texas 78744
(512)901-0900 ext. 6912
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Liz Chlipala
Sent: Thursday, April 21, 2011 12:59 PM
To: Nejat Yilmaz; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] decalcification of articular cartilage
We do a lot of IHC staining on bone and cartilage samples and we use 10% formic
acid for decalcification for immunohistochemistry. I'm afraid you may not be
able to turnaround this in a week. The tissue needs to be adequately fixed
prior to decalcification, and properly decaled. I would trim the tissue to
about 4 mm thick if you can on a saw you might be able to turn this around in
your time frame, but I have learned from experience if you rush decalcification
you have a greater chance of running into problems.
Liz
Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
PO Box 18592
Boulder, Colorado 80308
office (303) 682-3949
fax (303) 682-9060
www.premierlab.com
Ship to Address:
1567 Skyway Drive, Unit E
Longmont, Colorado 80504
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Nejat Yilmaz
Sent: Wednesday, April 20, 2011 8:12 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] decalcification of articular cartilage
Dear Members,
We're planning to study sheep knee articular cartilage with histochemistry and
immunohistchemistry. Proximal parts of the tibia bones fixing in formaldehyde
at the moment. We need an effective, quick and cartilage tissue protective
decalcification method. We'll perform only light microscopic examination in
this study and we have to complete the study within one week. In these
circumstances what are your advices?
Thanks in advance.
Dr. Necat Yilmaz
University of Mersin
School of Medicine
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