[Histonet] Histology Technical Specialist - Toronto

2014-10-30 Thread Matt Ward
Good morning, I hope everyone is doing great! We wanted to reach out regarding a new Histology Field Technical Specialist with a world leader in cancer diagnostics. The position will ideally be based in Toronto, and will provide histology technical expertise to internal and external

[Histonet] RE: QIHC

2014-10-30 Thread Bitting, Angela K.
Yes, it's part of the requirements to be able to take the exam. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Amy Johnson Sent: Wednesday, October 29, 2014 12:19 PM To:

[Histonet] RE: slides for IHC's

2014-10-30 Thread Rathborne, Toni
We have a Bond III, and although we use the Apex slides from Leica, we are not required to do so. We have 3 different manufacturer's slides validated should the Apex slides go on backorder. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu

[Histonet] RE: slides for IHC's

2014-10-30 Thread Matthew D. Roark
We are using our Leica Apex slides on the Ventana Ultra. No problems. Matthew Roark- HT/HTL(ASCP)CM Histology Specialist Saint Francis Medical Center 211 Saint Francis Drive Cape Girardeau, MO 63703 573-331-3982 mro...@sfmc.net http://www.sfmc.net -Original Message- From:

[Histonet] Tissue Processor Location in the Lab

2014-10-30 Thread Wheelock, Timothy R.
Hi Everyone: Even though many tissue processors are closed systems with fume filters, do people try to put them next to fume hoods or under some ventilation duct to add an extra layer of protection from solvent fumes? Thanks, Tim Wheelock Harvard Brain Tissue Resource Center McLean Hospital

[Histonet] RE: Tissue Processor Location in the Lab

2014-10-30 Thread Morken, Timothy
Tim, We have a separate room for the tissue processors. We have a chemical fume hood for pouring all chemicals into carboys via a funnel that is in a hole cut in the hood work surface, and a carboy directly beneath. We also have a fume extraction system above each tissue processor that is

[Histonet] Fibronectin

2014-10-30 Thread Sebree Linda A
Does anyone know of a reference lab doing this antibody on human FFPE slides? Thanks, Linda A. Sebree University of Wisconsin Hospital Clinics IHC/ISH Laboratory 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 ___ Histonet

[Histonet] RE: slides for IHC's

2014-10-30 Thread Maxim Peshkov
I found that my Leica Apex slides is not suitable for IHC. All the breast FFPE sections fall off after demasking into both FLEX buffers (high and low pH). I uses only Menzel Polysine slides with great success many years for any tissues for IHC. For HE and HC we uses ususal slides with gelatine

RE: [Histonet] Solvent Recyclers

2014-10-30 Thread Keyser Gerald T
GRAC http://www.newcomersupply.com/product/gravity-recycling-alcohol-cartridge-tissue-processor-grac-tp I've used this system. It takes quite a while to run. It works. But, there is one big problem. If there is a possibility of Xylene or Propar contamination, it will quickly ruin the filter

RE: [Histonet] Solvent Recyclers

2014-10-30 Thread Blazek, Linda
CBG http://www.cbgtechnologies.com/ Completely dependable. I've had mine for years with no problems at all. It runs forever! Linda -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Maryann Deathridge

RE: [Histonet] Solvent Recyclers

2014-10-30 Thread Weems, Joyce K.
Ditto Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.we...@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole

[Histonet] Help! Need someone to repair an IEC CTD Cryostat

2014-10-30 Thread Mercedes Gallagher
Hi, I'm having extreme difficulty finding someone who can work on the old IEC CTDs in Southern California. The issue isn't with the microtome, it's with the compressor/refrigeration. I've had a few people tell me they deal with these machines, only to find out after many hours of labor that

[Histonet] Paraformaldehyde fixation questions

2014-10-30 Thread Lewis, Patrick
After having a lot of problems using 100% Acetone as a fixative, I am going to try 4% paraformaldehyde in DPBS pH 7.4 for fixation of slides cut from fresh tissues snap frozen in OCT. My plan is to cut the slides at 5 or 6 uM and dry them for 2 hours in the hood, then fix them in the 4% p-f

RE: [Histonet] Solvent Recyclers

2014-10-30 Thread Bitting, Angela K.
ditto -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda Sent: Thursday, October 30, 2014 3:45 PM To: madeathri...@pastnashville.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet]

[Histonet] Antibodies for Microsatellite instability

2014-10-30 Thread Carlos Defeo
hi all, our laboratory in our country,Uruguay, is asked to perform Microsatellite instability on colon and endometrial carcinomas. I need your input on what vendors and clones would be the most reliable for these IHQ tests. Thanks in advance to all who could enlighten me on this point. Carlos