Re: [Histonet] Medical/health related post

I think it is rather unfair to accuse Dr. Raff of harassment or misusing this forum. I recall that when he first posted references to his blog he asked for the members' permission to do so, and he was given that permission. Rather than abusing the privilege of posting to Histonet, I think he

Re: [Histonet] aniline oil/Holzer stain

Aniline oil is more commonly known just as aniline. Sigma list it for sale at: http://www.sigmaaldrich.com/catalog/search?term=aniline=All=0=match%20partialmax=en=CA=product Bryan Llewellyn Susan Bachus via Histonet wrote: I'm trying to locate aniline oil for the Holzer glial fiber stain.

Re: [Histonet] Metabisulfite rinse for PAS reaction (potassium vs sodium)

You can use either the sodium or potassium salt. Both can also be used to make Schiff's reagent as well. In fact, many histotechs leave out the sulphite rinse step and simply rinse off in water and wash well with tap water. It seems to work just as well. Bryan Llewellyn Angela Lamberth via

Re: [Histonet] Elastic stain

Please read the article at :http://stainsfile.info/StainsFile/stain/elastic/elastic.htm There are numerous alternatives discussed there. I particularly like the Humberstone variant of iron resorcin fuchsin. Bryan Llewellyn Nirmala Srishan via Histonet wrote: Histonetters, Is there someone

Re: [Histonet] Metal embedding molds-large

Sorry! That should be TSP - trisodium phosphate - not TCP, which might make it worse. Bryan Bryan Llewellyn wrote: This used to be a common problem years ago. It is due to crud buildup on the metal. Boil them with TCP for half an hour, then thoroughly wash them in cold water. Coat them

Re: [Histonet] Metal embedding molds-large

This used to be a common problem years ago. It is due to crud buildup on the metal. Boil them with TCP for half an hour, then thoroughly wash them in cold water. Coat them with a VERY light smear of glycerol before you use them, preferably each time. That should help. Bryan Llewellyn. Diane

Re: [Histonet] Wright-Giemsa for sections?

Go to http://stainsfile.info/StainsFile/stain/oversight/romanowsky.htm Bryan Llewellyn Tyrone Genade via Histonet wrote: Hello, Can the Wright-Giemsa stain be used on fixed, paraffin embedded sections? Does anyone have a protocol? I want to examine hematopoietic tissue of fish, i.e. the

Re: [Histonet] Iron Stain

1% neutral red in 0.01% acetic acid (1 mL of 1% per 100 mL stain) works, as does 1% safranin. Both are stable, although should be filtered from time to time. Stain 1 minute, water rinse, ethanol, xylene and mount should work. I had one pathologist who preferred a very light progressive H She

Re: [Histonet] Frozen sections and cold acetone...

Cold acetone, and cold ethanol, were used to fix tissues because they left enzymes unaffected and still demonstrable. This was in the early days of enzyme histochemistry. Pearse' Histochemistry: Theoretical and applied,3rd edition, volume 1, page 85 discusses it. I could send a scan if you

Re: [Histonet] (no subject)

All of the Picro-Mallory variants are trichrome stains. An explanation of how they work is here: http://stainsfile.info/StainsFile/theory/tri_gen.htm http://stainsfile.info/StainsFile/stain/fibrin/fibrin.htm Follow the links as well for added information. Bryan Llewellyn Пешков Максим via

Re: [Histonet] Muscle Enzyme Histochemistry

Hi, I presume you are talking about light microscopy. Glutaraldehyde is often used for electron microscopy. If that is the case, ignore this. Glutataraldehyde fixes similarly to formalin, so the morphology should not be much different. However, it leaves the tissue with free aldehyde groups

Re: [Histonet] 5% Chromic acid disposal

I suspect disposal might vary depending on the State. I live in British Columbia and we had permission from out city (Prince George) to use a dribble tank with lots of water and flush them into the local river (The Fraser), but I opted to collect all the toxic chemicals and ship them

Re: [Histonet] New lab set up

I have sectioned both standing and sitting, and much prefer sitting. However, due to back problems I found it necessary to have a stand built for the microtome so that when I was sctioning I reached slightly upwards rather than crouched over. This all but eliminated the strain on my back. The

Re: [Histonet] contaminating patient requesition forms

We used to have a form with two sheets. The front sheet was a "magic carbon" so the patient information was automatically transferred. During booking in of the specimen, we separated the sheets. The back sheet accompanied the specimen for the gross description and the top sheet went directly

Re: [Histonet] On-line references

StainsFile is still available at http://stainsfile.info In the downloads section there is a scanned, corrected and reformatted copy of the Microtomist's Vade Mecum, 7th edition. This text covers other areas as well as medical and is in the public domain in the US and Canada, and likely

Re: [Histonet] Bone decalcification

Use surface decalcification: Surface the block, then remove from the microtome. Do not use the microtome for another block, so as to avoid adjusting the position of the block face to the knife. Place the block face down in 4% nitric acid for 2 hours or longer. Rinse the block and cool

Re: [Histonet] Fixative in diff-quick

Diff Quick appears to be a modified Field's stain. The blue dye in the methanol is one of the modifications. Field's stain gives much the same staining, so using plain methanol should be of no concern. The simplest way to find out is surely to try it and see.

Re: [Histonet] Jones' Methenamine Silver Stain for Basement Membranes of Kidney - Issues and Questions

Hi, Try the method given in StainsFile at: http://stainsfile.info/stain/metallic/jones.htm Bryan Llewellyn Hood, Jordan via Histonet wrote: Hello, I'm new to histology (and new to histonet), and I work in a small histology lab specializing in animal tissues that receives