[Histonet] CD8 IHC on FFPE mouse tissue -what your experience?

Hi all, I have a project that will be looking at CD8 expression in some FFPE murine tissue. Past discussions here have mentioned the 14-0808 CD8a antibody from eBiosicence. For those of you have had the opportunity to use this antibody I would like to hear your experiences/ recommendations

Re: [Histonet] autoradiography frozen human brain sections fall off, white matter issue

Hi Esther, We do a lot of autoradiography on rodent, primate and human brains sections. Our protocol is similar to yours except we do not dry the slides after incubating in the buffer and before adding the radiotracer. The experiment is performed with the slides in a rack and totally immersed

Re: [Histonet] Tissue request....

Rachel, If you have funding to purchase you can check out these biorepositories... Asterand Bioscience http://www.asterandbio.com/ National Disease Research Interchange http://ndriresource.org/ Cooperative Human Tissue Network http://www.chtn.nci.nih.gov/ Analytical Biological Services

Re: [Histonet] DIF on paraffin embedded tissue

Also agree. Lots of people do it. Search PubMed, J. Histo Cyto. If you have the appropriate filters, using Cy dye secondary's can help move away from any autofluorescence. Brett Brett M. Connolly, Ph.D. Prin. Scientist, Translational Biomarkers - Imaging Merck & Co., Inc. PO Box 4, WP-44K

[Histonet] PDGFR beta antibody for IHC

Anyone have a favorite for rodent FFPE tissue? Thanks, Brett Brett M. Connolly, Ph.D. Prin. Scientist, Translational Biomarkers - Imaging Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 brett_conno...@merck.com T- 215-652-2501 F- 215-993-6803 Notice:

[Histonet] HSV-TK antibody for IHC

Anyone know of an anti-HSV tyrosine kinase antibody suitable for IHC? The ones I am finding are WB and ELISA only. Thanks, Brett Brett M. Connolly, Ph.D. Prin. Scientist, Translational Biomarkers - Imaging Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486

[Histonet] CD3 antibody for FFPE mouse tissue

Hi all, Looking for recommendation for an anti-mouse CD3 antibody for FFPE sections. I have some old Histonet messages referring to a Neomarkers RB-360 ab which I found through Thermo, but I would like to hear about more recent experiences/recommendations Thanks as always, Brett Brett M.

[Histonet] Thanks! CD3 antibody for mouse IHC

Thanks to all who replied to my CD3 query! The responses were just what I was hoping for. Best regards, Brett Brett M. Connolly, Ph.D. Prin. Scientist, Translational Biomarkers - Imaging Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486

[Histonet] Masson's Trichrome or Sirius Red on frozen tissue

Has anyone preformed these stains of frozen sections??? Looking for any tips. I'll have fresh frozen tissue that can be fixed after sectioning and mounting onto the slide. Thanks, Brett Brett M. Connolly, Ph.D. Prin. Scientist, Translational Biomarkers - Imaging Merck & Co., Inc. PO Box 4,

Re: [Histonet] Lab Coats and OCT

I am a fan of Cryo-Gel - highly viscous which facilitates easier tissue orientation. We get it from Electron Microscopy Sciences (#62806-01). Try it, you'll like it. Brett Brett M. Connolly, Ph.D. Prin. Scientist, Translational Biomarkers - Imaging Merck & Co., Inc. PO Box 4, WP-44K West

Re: [Histonet] HT Exam

For $30.00 you can get them from ASCPprobably a good investment !! http://www.starttest.com/7.2.0.0/cart.aspx?program=ASCPPractice Brett Brett M. Connolly, Ph.D. Prin. Scientist, Translational Biomarkers - Imaging Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486

Re: [Histonet] JOH journal collection --it's claimed

All gone. Brett Brett M. Connolly, Ph.D. Prin. Scientist, Translational Biomarkers - Imaging Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 brett_conno...@merck.com T- 215-652-2501 F- 215-993-6803 Notice: This e-mail message, together with any

[Histonet] JOH journal collection

HI all, Is anyone interested in old issues of JOH? My collection spans 1997 - 2016. I am missing a few (~ 7). Let me know and I'll send you a list of what I have. Brett Brett M. Connolly, Ph.D. Prin. Scientist, Translational Biomarkers - Imaging Merck & Co., Inc. PO Box 4, WP-44K West Point,

[Histonet] Books and Atlases- Part 2 (with pricing and more books added)

Hi all, Thanks for all the responses to my previous post. I really want to pass these along rather than trash them. I updated the list and included my asking price. In addition I have a second list of free books - provided you purchase a book or books (one free book per purchase). I'll pay

[Histonet] Part 3 - Book and Atlases they're going fast

Hi all, Thanks for all of your responses! The list has shrunk considerably. Here is what left. I forgot to add the Sheehan & Hrapchak classic: Theory and Practice of Histotechnology (this one is a little worn---go figure). Atlas of UroSurgical Anatomy; ISBN 0-7216-3955-0 $30.00

[Histonet] Part 4 - Book and Atlases - Final

Hi all, Thanks for the tremendous response! I am delighted that 38 books and atlases have found new homes. I only have 2 left if there is still interest in these. Sorry - no more freebies available. Primate Brain Maps: Structure of the Macaque Brain; ISBN 0-444-50415-X $50:00 Atlas of