[Histonet] Attwood's tips please

Hello, I'm trying to work up an Attwood's method but would be keen to get a guide on time for the differentiation step in tartrazine solution. The method I'm using suggests 20 minutes at the longest with regular microscope checks. I'm currently at about 4 hours with only slight loss of colour

[Histonet] Freezing artefact

Good Afternoon all, I have a couple of cases where my post mortem samples have been accidentally frozen in Neutral Buffered Formalin. Naturally this has led to pretty striking artefact in the tissue. I was wondering if anyone who perhaps has more experience of dealing with frozen tissue has