[Histonet] GMS and Gram stains

Hi All Netters, I need to get a better GMS and Gram stain. If anyone could share their protocols where they get the solutions that would be fantastic. Thanks, Mehndi Helgren Dominion Pathology Labs Norfolk, VA (AKA Hurricane Central) :) ___

[Histonet] GI biopsies processing

Hi All, Can anyone share their processing set up for GI biopsies? No matter what I do, our tissue seems dry. Any input would be great. Thanks in advance, Mehndi Helgren Dominion Pathology Labs. ___ Histonet mailing list

[Histonet] Freeze Spray Ban

Hi All, Just got an alert stating that the EPA is banning HFCs found in Freeze Spray effective July 20th. Does anybody use a spray that does not contain this banned chemical? If so, please let me know where you get it. Thanks, Mehndi Helgren Dominion Pathology Labs "DPL"

[Histonet] Herpes Zoster (VZV)

Hi everyone- Will anyone share where they get their Herpes Zoster antibody from for FFPE? I currently get it from abcam and run them on a Ventana Ultra. It seems once a year or so, the antibody just stops working and I have to call abcam and they send me a new one and it usually works. This

[Histonet] Freeze Spary Ban continued

FYI: Mercedes Medical DOES contain the banned chemical. StatLab's 1,1,1,2 Tetrafluoroethane is banned. This is also called HFC 134a Still checking other brands. -Mehndi ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

[Histonet] HT needed in Norfolk, VA

Hi all, I have a position open for Histo Tech with a private dermpath lab in Norfolk, VA. Must have HS/GED be ASCP certified as a HT or HTL. Experience needed in routine and IHC. Full time M-F with morning hours. Fax confidential resume to 757-664-9122 Attn: Administrator

[Histonet] PGP 9.5 at 50 microns

Hi All, I need help. My doctors want to start doing some diabetic nerve testing. They need a PGP 9.5 IHC done on a frozen section cut at 50 microns. Does anybody have any experience on something like this? If so, I would love some help. Thanks, Mehndi Helgren Dominion Pathology Labs

[Histonet] Histopathology positions available in central Massachusetts

Because of a continued increased in workload, Mass Histo is expanding our day shift and soon will be adding a second shift. We're looking for experienced grossing technicians, histologists, specimen procurement techs (with knowledge of CPT coding) and lab aids. We offer a pleasant working

[Histonet] HT job in VA

We have an HT position open in Norfolk, VA. M-F 6am-2Pm. Must be certified. No recruiters please. Email resume to hi...@pathlab.us or fax to 757-664-9122 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

[Histonet] benchtop fume hoods

Hi all, Can anyone share what type of benchtop fume hoods they are using in the lab? This would be for manual coverslipping and grossing. Thanks in advance! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

Re: [Histonet] Histonet Digest, Vol 170, Issue 4 Extra unstained paraffin embedded tissue slides (Kristina Johnson)

Hi Kristina, You can always cut a section from a blank block of wax and cover over the unstained uncoverslipped sections and let them air dry before filing them away. Regards Stuart Beaver BScHons Head of Veterinary Histology Histology Department NationWide Laboratories (A trading business

[Histonet] Fridge and freezer temps

Hi All, I'm trying to figure out how everyone keeps their refrigerator and freezer temperatures in a certain range. I have 2 house refrigerators with freezers on top. We have the min/max thermometers. I cannot get the freezer to stay between 0 and -8 degrees without affecting the fridge

Re: [Histonet] Giemsa vs Diff Quik

Hi, Have you tried leishman staining? I have an automated protocol for he thermo gemini if you want one? Failing that I do have the manual step protocol and all you need is a jar of leishman powder, methanol and pH6.8 phosphate buffet tablets Stuart Beaver BSc(Hons) Head of Veterinary

Re: [Histonet] Elephant Tissues

Hi Jennifer, If it is skin extending the fixing and processing time should cure any processing issues. If after this it’s still not working a short pre fix in khales fixative and surface softening before sectioning with 0.02% sodium hydroxide may help. Hope this helps! Stuart Beaver

[Histonet] Ventana Red Detection Kits

HELP!!! Ventana is out of all Red detection kits until the end of the month. We are a small lab that only uses the red detection. Is everyone switching to DAB or are there other options? If switching to DAB, are you revalidating every antibody? And how many cases per antibody? Thanks for

[Histonet] Silver waste

Hi All. Happy Monday! Would anyone share how they collect, store, and dispose of silver waste from manual special stains such as GMS, Melanin Fontana-Masson, and Calcium Von Kossa? Thanks in advance! ___ Histonet mailing list

[Histonet] Special GI fixative

Hi all, Does anyone use a special fixative for their GI specimens? I was thinking of trying something called GI fix. Ingredients are basically formalin and zinc chloride. Our tissue is always super brittle and dry even with a very short processing schedule. Any thoughts would be greatly

[Histonet] Leica SCN400 slide carriers

Hi Histonet, I would like to ask if anyone has (or knows who may have) any spare slide carriers for the Leica SCN400 scanning microscope. Is anyone currently using this model and how does it perform? Best wishes Stuart Beaver BSc(Hons) Head of Veterinary Histology/Cytology +447568543761

[Histonet] table for cutting

Does anyone use a table to put their microtome and cut? I'm trying to find one and I want to make sure that it is stable enough for cutting. Thanks, Mehndi Helgren Dominion Pathology Laboratories 733 Boush St. Suite 200 Norfolk, VA 23510 757-664-7901

[Histonet] Not DAB IHC waste

Hi all, Does anyone use only red or magenta detection for IHCs instead of DAB? We do not use DAB and are trying to figure out if our waste is hazardous or not. Any insight would be helpful. Thanks, Mehndi Helgren ___ Histonet mailing list

[Histonet] fume hoods for grossing

Hi Histonetters, I'm trying to see what kind of exhaust/fume hoods people are using in small grossing labs. We are moving into a new space and I want to make sure the ventilation at the gross board is adequate. I don't think an actual grossing station is in the budget. Thanks, Mehndi

Re: [Histonet] Histonet Digest, Vol 196, Issue 3

Re: Histonet digest Incomplete sectioning of blocks. I had to manage this situation a few year back and found that the best place to capture this is at the waterbath. QC reviews I.e numbers of return slides categorised into fault type can be collated over a month and displayed easily to a team

[Histonet] Leica ST5020 vs Spectra

Hi all, We have a Leica ST5010 that we have had forever and love. We have gotten a lot busier and need to eliminate the backup we currently get every day waiting to load the racks. I think we need an extra oven or 2. So we are looking into updating and will probably go with the ST5020 or

[Histonet] comparable eosin

Hi everyone, We have always got our Eosin from BBC Biochemical. Well, they were bought out by StatLab and I am trying to find a comparable Eosin from what we were using. We used "Skin Eosin" catalog #3680 composed of: water, denatured ethanol, eosin y, and glacial acetic acid. I've done

[Histonet] Lyme disease

Does anyone perform IHC for Lyme disease (Borrelia burgdorferi) on paraffin blocks? I'm looking for a good antibody and a place to buy control slides. Thanks to all! Mehndi Helgren Dominion Pathology Laboratories 733 Boush Street Suite 200 Norfolk, VA 23510 757-664-7901

[Histonet] cryostat

Hello, Trying to get everyone's opinion on cryostats. We have had the Leica CM1850 forever and it is not being supported by Leica anymore. Thinking of trying a different company maybe Tanner or Rankin. What does everyone use and like. We do not cut Mohs, only DIFs. Maybe 1 a day. Thanks

Re: [Histonet] Moderate vs High Complexity for routine histology

Histology is considered moderate by CAP and CLIA -Original Message- From: James P Montgomery via Histonet Sent: Friday, October 20, 2023 4:04 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Moderate vs High Complexity for routine histology Hello, Is routine histology