[Histonet] Looking for a Histology Workshop

Hi Everyone, I am looking for workshop or class for Histology/Histotechnology that lasts anywhere from 1 week to 3 months in the U.S. Preferably in-person and not online. If you have any suggestions or contacts, please let me know. Thank You, Jessica

Re: [Histonet] Looking for a Histology Workshop

Ph.D. George Mason University Fairfax, VA 22030- ________ From: Jessica Riggleman via Histonet <histonet@lists.utsouthwestern.edu> Sent: Wednesday, February 8, 2017 1:07:24 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Looking for a Histolog

[Histonet] New Bone vs. Old Bone Stain

Hello, Can anyone recommend a stain that differentiates new (woven) bone vs. old (lamellar) bone? We are looking for bone growth, and biomaterial was used to facilitate. We are using a rabbit femoral defect and a rabbit PLF model. Protocols/Input for MMA thin/thick sections and formalin fixed

[Histonet] TRAP Stain

Can anyone recommend a TRAP (tartrate resistant acid phosphatase) protocol for formalin fixed paraffin embedded Rabbit PLF specimens? Or a kit to use? Thank you, Jessica _ Jessica Riggleman | Research Associate Globus

Re: [Histonet] TRAP Stain

Also if anyone has any photos of slides stained that would be great. We are looking for osteoclasts. _ Jessica Riggleman | Research Associate Globus Medical, Inc. Valley Forge Business Center 2560 General Armistead Avenue |

Re: [Histonet] Egg Whites and IHC Staining

ab.com Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -Original Message- From: Jessica Riggleman via Histonet [mailto:histonet@lists.utsouthwestern.edu] Sent: Monday, September 12, 2016 8:05 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet

[Histonet] Egg Whites and IHC Staining

Hello, We are cutting large Rabbit PLF formalin fixed paraffin embedded sections. We have had issues staining H and Goldner’s Trichrome. Egg whites is working as a great slide adhesive for these two, but not so much for IHC Staining (RAM 11 specifically). Any ideas/suggestions on how to get

[Histonet] Knife Sharpening Service

Can anyone recommend a good Knife Sharpening service around the east coast? We are located in the PA area. We have tried CL Sturkey and Dorn & Hart Microedge. It doesn't necessarily need to be East Coast.. We are just looking for ~1 Week turn around time. Thank you, Jessica

[Histonet] Our Lab Needs a Microtome - Suggestions

Hello, I am looking for a similar machine to the Reichert-Jung Polycut S Microtome. I am looking to cut MMA thin sections on rabbit femoral condyles (somewhat larger than usual specimen size). If anyone can recommend a newer microtome or is looking to sell, that would be great. I am unaware of

Re: [Histonet] Faxitron

Hi, We use a Faxitron machine in our lab. _ Jessica Riggleman | Research Associate Globus Medical, Inc. Valley Forge Business Center 2560 General Armistead Avenue | Audubon, PA 19403 Ph: (610) 930-1800 ext. 2583 | Fax:

[Histonet] Vortex Mixer - Sale?

Hello, Does anyone have an old vortex mixer they do not need, such as the one below: http://www.ebay.com/itm/Fisher-G-560-Vortex-Genie-2-Lab-Mixer-Disc-Platform-Shaker-12-812-Ref-A-/282149263394?hash=item41b1681822:g:IwIAAOSwZVlXvu9V I am willing to pay you and willing to pay shipping. Thank

[Histonet] Positive Control for TRAP Staining

Hello, Does anyone have a suggestion for a good positive control for TRAP staining? Thank you, Jessica Riggleman _ Jessica Riggleman | Research Associate Globus Medical, Inc. Valley Forge Business Center 2560 General

Re: [Histonet] siphon drum pump

Lauren, What is your pump made out of? _ Jessica Riggleman | Research Associate Globus Medical, Inc. Valley Forge Business Center 2560 General Armistead Avenue | Audubon, PA 19403 Ph: (610) 930-1800 ext. 2583 | Fax:

Re: [Histonet] Question on Sanderson Rapid Bone Stain

RBS is the same as Methylene Blue, I believe. _ Jessica Riggleman | Research Associate Globus Medical, Inc. Valley Forge Business Center 2560 General Armistead Avenue | Audubon, PA 19403 Ph: (610) 930-1800 ext. 2583 | Fax:

Re: [Histonet] Microtome Cleaning Company and What to do with Old Metal Blades

Hello, I send mine out to IMEB in California. You may be able to sell the blades on Ebay. Thank you, Jessica _ Jessica Riggleman | Research Associate Globus Medical, Inc. Valley Forge Business Center 2560 General Armistead

[Histonet] Paraffin Removal

Hi Everyone, Please tell me your tricks of removing paraffin from containers, lab benchtops, etc….. I would really appreciate it! Thank you, Jessica _ Jessica Riggleman | Research Associate Globus Medical, Inc. Valley Forge

[Histonet] Macrophage Stain

Hello, Could you please help me find a macrophage stain that will work with sheep (specifically sheep laminectomy sections)? We are thinking RAM-11 or HAM-56, but unsure. Also, what about Rabbit PLF? Please note both are embedded in mega cassettes for paraffin processing. Thank you, Jessica

[Histonet] Polycut Microtome - MMA Thin Sections - Help!

Hello, I am looking to use a Reichert-Jung Polycut S Microtome to cut MMA thin sections (5-10 microns). However the specimens I am cutting are very small. How do you go about gripping such small sections on such a microtome? I have tried using a dental mount to adhere the specimen to a blank

Re: [Histonet] High/Low thermometed

Hi Amanda, We use T, they record temperature and humidity. http://www.tandd.com/product/tr7wfnw_series.html -Jessica _ Jessica Riggleman | Research Associate Globus Medical, Inc. Valley Forge Business Center 2560 General

[Histonet] Coverslipping

Hello, Would anyone mind sharing their procedure after coverslipping a slide? Do you let the slide sit and dry? Do you use xylenes to wipe of the extra mounting medium immediately? I noticed after storing the slides in a slide box, a lot of dust collects. Is this because of the xylenes?

[Histonet] Microscope Camera

Hi Everyone, I am looking for a camera for a microscope (specifically for an Olympus BX41) to look at cellular detail. If you have one that is not in use please let me know. Thank You, Jessica _ Jessica Riggleman |

[Histonet] Ultrasonic Cleaning Bath

Hello, I am looking for a used ultrasonic cleaning bath and a used plate shaker. If you have either one laying around your lab please let me know! Will pay. Thank You, Jessica Riggleman _ Jessica Riggleman | Research

[Histonet] Umbilical Cord Embedding

Hello, Does anyone have any experience embedding umbilical cord tissue? Preferably paraffin but we may try plastic as well. Any help/protocols/etc. would be appreciated. Thank You, Jessica _ Jessica Riggleman | Research

[Histonet] Goldner's Trichrome Thick Sections

Hello, Looking for a protocol for staining thick plastic methyl methacrylate sections (100-150 um). Goldner’s or any trichrome stain would work. Thank You! Jessica _ Jessica Riggleman | Research Associate Globus Medical,

[Histonet] MMA or Epoxy Embedding

Hi Everyone, I am trying to embed screws in plastic (in the hopes to see breakage in the screw, etc). Right now I use specifically a mixture of methyl methacrylate, poly methyl methacrylate, and benzoyl peroxide. However this usually takes a few weeks to embed/dry. I am looking for something a

Re: [Histonet] Histonet Digest, Vol 177, Issue 5

We let our tough blocks soak in Downy fabric softener face down (depending, 10-30 minutes) and then try to section, sometimes use ice, then coat our slides in egg whites if they are tough to adhere. _ Jessica Riggleman |

[Histonet] New Bone Stain

Hello, I am trying to assess new bone vs. old bone in an animal model. Any suggestions on thick section stains? I have heard Van Gieson's? Thank You, Jessica _ Jessica Riggleman | Research Associate Globus Medical, Inc.

Re: [Histonet] New Bone Stain

the mineralized bone black and a basic fuschin counter stain the osteoid will stain deep pink. Get Outlook for iOS From: Jessica Riggleman via Histonet Sent: Monday, July 9, 2018 1:17 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] New Bone