Re: [Histonet] Freeze Spray Not Sold as Case Happy Friday

I heard everything on the intervention is true, is that true? Paula ;-) Sent from my iPhone On Aug 21, 2015, at 9:16 AM, Jay Lundgren via Histonet histonet@lists.utsouthwestern.edu wrote: There's this cool thing called the Interwebz now?

Re: [Histonet] Slides stuck together

Hi Tonya, Soak the brick-o-slides in xylene until they unstick from each other. This can take over a week, you must be patient. The slides will break if you try to force them apart. Paula Sent from my iPhone On Aug 10, 2015, at 6:51 AM, Abbott, Tanya via Histonet

Re: [Histonet] Ventana Retic Stain Quality

I was told by the technical specialist to make the wash solution fresh before use. There has been a contamination caused by growth of organisms in the wash if it sits around too long between uses. Paula :-) Sent from my iPhone On Jul 16, 2015, at 10:14 AM, Blake Taylor via Histonet

Re: [Histonet] Water for H Stainers?

And the winner for the water of choice for H strainers is.. What ever you have available. Tap, tap with a filter, deionized. It all depends on the water quality in your area. Thanks to all who replied. Paula Sent from my iPhone > On Sep 27, 2016, at 9:54 AM, Elizabeth Chlipala

Re: [Histonet] RELIA Histology Careers Bulletin - Live where you vacation 8-1-2017

My comment was meant in jest. I live in San Diego, so Modesto is not a city I would vacation. Modesto probably has many nice features. Paula Sent from my iPhone > On Aug 2, 2017, at 6:32 AM, Pam Barker via Histonet > wrote: > > Hi Histonetters! > I

Re: [Histonet] Microscope objectives

Also, the manufacturers make the threads different so what works on one brand won’t work on another. They even change the threading between models so you have to buy new objectives. Paula Sent from my iPhone > On Jun 19, 2018, at 6:44 AM, Rene J Buesa via Histonet > wrote: > > Not all

Re: [Histonet] p2y12 cryo rat problem high background

It could be the concentration of the antibody is too high. Have you tried a lower dilution? What species made the antibody? I know that sounds a bit basic but I know that I have used a mouse or rat antibody by mistake. Paula Sent from my iPhone > On Nov 15, 2018, at 4:44 AM, Kooijman,

Re: [Histonet] New York Qualifications for Histology Supervisor

This is an issue that continues to pop up. It all starts with the CLIA regulations that do not recognize histology professionals as equal to other laboratory professionals. Until the feds change CLIA we will keep having this problem. I do believe that NSH is petitioning on our behalf to

Re: [Histonet] Tissue processing

I favor the VIP too. My lab has 4 VIPs and 1 Leica Peloris. The VIPs can process more cassettes on a run and cost a lot less than the Peloris. Paula Sent from my iPhone > On Mar 6, 2020, at 12:34 AM, warda hassan via Histonet > wrote: > > Hello to all histonets > > Advanced thanking

Re: [Histonet] Changing to reagent alcohol

Hi Carole, Sorry but you need to validate the change completely. Reagent alcohols are blends that sometimes don’t even contain ethanol. You need to demonstrate that it doesn’t affect routine H, special stains, and IHC. Plus you have to test the major tissue types you routinely work on.

Re: [Histonet] Warm formalin

Hi Erin, Often heat is applied to formalin to speed up fixation. That said there is probably a temperature point where it goes from fixing tissue to cooking it. Paula Sent from my iPhone > On Jul 21, 2020, at 6:14 PM, Martin, Erin via Histonet > wrote: > > Hello everyone! > > We have

Re: [Histonet] Tissue processor errors, failures and what to do

Hi Garrey, The answer is “it depends”. What you do when a processor fails depends on the failure point. If the tissue is still in dehydrant it gets treated differently than if it fails in the intermediate solvent. Paula Sent from my iPhone > On Jul 4, 2020, at 10:08 AM, Garrey Faller

Re: [Histonet] Automated Coverslippers

Happy Friday Eve, We use the Sakura glas2 cover slipper. We run hundreds of slides through them every day with very few problems. Any automated coverslipper can have issues. A word of warning- watching the g2 do its thing is hypnotic. Paula Sent from my iPhone > On May 13, 2021, at

Re: [Histonet] Antibody diluent

I’m not completely sure but I think you can do either a mini validation or a comparison validation to show there’s no difference between the results. I think CAP has a requirement for what to do when reagents change. Paula Sent from my iPhone > On Mar 25, 2021, at 6:19 AM, Jeanine

Re: [Histonet] Tape Transfer Methods For Cryosectioned Brains

Good Morning Heather, I have some questions about how you cut frozen brains. What temperature are you cutting at? How thick are your sections? How are your samples frozen? Flash freezing, slow freezing, iso-pentane in LN2? Your answers may provide clues to help you get better

Re: [Histonet] Filed slides sticking together

We used to use a drying oven set at 37 degrees C. We stopped using it because some people thought it smelled, plus our slide volume increased to the point where all the flats couldn’t fit. Now we dry them for two days, minimum, before filing. I prefer to let them dry for at least 4 days.

Re: [Histonet] Tape Transfer Methods For Cryosectioned Brains

Hi Heather, I can see why you’re having trouble. 30 micron sections are inherently unstable. Like paraffin, the thicker a section is the more difficult it is to cut. Plus since your practice samples are old they tend to be more brittle. Try cutting at 5 microns and see what happens.

Re: [Histonet] tissue cassettes

I'm old, old school too.  I prefer the paraffin pool myself, it helps prevent the bon-bon effect.  I had a vendor rep tell me the holding bins were designed for molten paraffin.Probably what happened over the years is that if there's too much molten wax in the bin, it over flows when the basket

Re: [Histonet] tissue cassettes

Hi Carl,The bon-bon effect.  This can happen if the wax used for processing is different from the wax used for infiltration or when the tissue gets cold before embedding.Either way, because the tissue is cold - the embedding wax doesn't blend with the tissue wax, creating the hard outer wax