Re: [Histonet] Tissue Processor Down Question

[Diana Martinez-Longoria via Histonet]

Okay, thank you for your advice.

Thank you,
Diana Martinez-Longoria
El Centro Regional Medical Center
Lead Histotechnician (ASCP)cm
Laboratory - Pathology Department
1415 Ross Ave | El Centro, CA  92243
760.339.7267: Fax: 760-3394570
 diana.martinez-longo...@ecrmc.org



-Original Message-
From: Gudrun Lang [mailto:gu.l...@gmx.at] 
Sent: Thursday, March 14, 2024 7:28 AM
To: Diana Martinez-Longoria
Cc: histonet@lists.utsouthwestern.edu
Subject: AW: [Histonet] Tissue Processor Down Question

Warning! This email was sent from outside your organization. Do not click or 
open attachments if you don't recognize the sender. 

Depending on the time the cassettes were in clearing medium, I would transfer 
them into molten paraffin at least for double of the time in the processor. And 
then embed etc.
Try a few blocks to see, if infiltration was sufficient. If cutting is 
hampered, prolong the time in paraffin.
Gudrun

-Ursprüngliche Nachricht-
Von: Diana Martinez-Longoria via Histonet 
[mailto:histonet@lists.utsouthwestern.edu]
Gesendet: Donnerstag, 14. März 2024 13:59
An: histonet@lists.utsouthwestern.edu
Betreff: [Histonet] Tissue Processor Down Question
Wichtigkeit: Hoch

Good morning,

Our VIP5 tissue processor went down and our tissue cassettes were in clearing 
solution. What is the best media to put them while we wait for the tissue 
processor to be repaired, please let me know?
Thank you,
Diana Martinez-Longoria
El Centro Regional Medical Center
Lead Histotechnician (ASCP)cm
Laboratory - Pathology Department
1415 Ross Ave | El Centro, CA  92243
760.339.7267: Fax: 760-3394570
 diana.martinez-longo...@ecrmc.org<mailto:diana.martinez-longo...@ecrmc.org>




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Re: [Histonet] Tissue Processor Down Question

[Gudrun Lang via Histonet]

Depending on the time the cassettes were in clearing medium, I would
transfer them into molten paraffin at least for double of the time in the
processor. And then embed etc.
Try a few blocks to see, if infiltration was sufficient. If cutting is
hampered, prolong the time in paraffin.
Gudrun

-Ursprüngliche Nachricht-
Von: Diana Martinez-Longoria via Histonet
[mailto:histonet@lists.utsouthwestern.edu] 
Gesendet: Donnerstag, 14. März 2024 13:59
An: histonet@lists.utsouthwestern.edu
Betreff: [Histonet] Tissue Processor Down Question
Wichtigkeit: Hoch

Good morning,

Our VIP5 tissue processor went down and our tissue cassettes were in
clearing solution. What is the best media to put them while we wait for the
tissue processor to be repaired, please let me know?
Thank you,
Diana Martinez-Longoria
El Centro Regional Medical Center
Lead Histotechnician (ASCP)cm
Laboratory - Pathology Department
1415 Ross Ave | El Centro, CA  92243
760.339.7267: Fax: 760-3394570
 diana.martinez-longo...@ecrmc.org<mailto:diana.martinez-longo...@ecrmc.org>




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[Histonet] Tissue Processor Down Question

[Diana Martinez-Longoria via Histonet]

Good morning,

Our VIP5 tissue processor went down and our tissue cassettes were in clearing 
solution. What is the best media to put them while we wait for the tissue 
processor to be repaired, please let me know?
Thank you,
Diana Martinez-Longoria
El Centro Regional Medical Center
Lead Histotechnician (ASCP)cm
Laboratory - Pathology Department
1415 Ross Ave | El Centro, CA  92243
760.339.7267: Fax: 760-3394570
 diana.martinez-longo...@ecrmc.org




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Re: [Histonet] Tissue Processor Schedule Validations

[Cooper, Brian via Histonet]

WOW!  I came back from lunch and I have seven responses already!  Everyone said 
the same thing, which was my game plan, take punches from larger samples!  
Happy Friday Eve everyone!   

Thanks so much Histonet!  

Brian

-Original Message-
From: Cooper, Brian via Histonet  
Sent: Thursday, March 23, 2023 11:54 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Tissue Processor Schedule Validations (EXTERNAL EMAIL)

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Good afternoon Histonet,

We're going to be validating a new tissue processor (Peloris 3) in the coming 
months, and I'm curious how people have validated small tissue processing 
protocols (GI bx's, liver/renal needle cores).  Larger tissues are much easier 
to do because we can readily gross duplicate sections. Obviously we can't adopt 
this approach for smaller samples because they're entirely submitted.  I have a 
game plan in mind, but would love some additional input! How'd you do it?

Thanks,

Brian D. Cooper, HT (ASCP)CMQIHCCM| Histology Supervisor Department of 
Pathology and Laboratory Medicine Children's Hospital Los Angeles
4650 Sunset Blvd MS#43- Los Angeles, CA 90027
Ph: 323.361.3357
bcoo...@chla.usc.edu<mailto:bcoo...@chla.usc.edu>

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Re: [Histonet] Tissue Processor Schedule Validations

[Paula Sicurello via Histonet]

The one thing that bothers me about the preloaded programs is the paraffin 
temperature is set at 65 degrees C.  That's close to 10 degrees higher than the 
melting point of our paraffin.
Does anyone know why the temp. is so high?  To me, a temp that high will cause 
problems with IHC and small biopsies.  
Has anyone changed it?  Or do folks go along with it just because it's already 
there? 
I inherited that temp, to change it now would be pain since it is the one we 
use the most.  If I validated our current Peloris 3, I would have set the wax 
temperatures at 60 degrees.
Sincerely,
Paula Sicurello 

On Thursday, March 23, 2023 at 11:54:44 AM PDT, Cooper, Brian via Histonet 
 wrote:  
 
 Good afternoon Histonet,

We're going to be validating a new tissue processor (Peloris 3) in the coming 
months, and I'm curious how people have validated small tissue processing 
protocols (GI bx's, liver/renal needle cores).  Larger tissues are much easier 
to do because we can readily gross duplicate sections. Obviously we can't adopt 
this approach for smaller samples because they're entirely submitted.  I have a 
game plan in mind, but would love some additional input! How'd you do it?

Thanks,

Brian D. Cooper, HT (ASCP)CMQIHCCM| Histology Supervisor
Department of Pathology and Laboratory Medicine
Children's Hospital Los Angeles
4650 Sunset Blvd MS#43- Los Angeles, CA 90027
Ph: 323.361.3357
bcoo...@chla.usc.edu

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Re: [Histonet] Tissue Processor Schedule Validations

[Thomas Podawiltz via Histonet]

I ordered small punch biopsy kits and made my own small biopsies from remnant 
tissue. Renal biopsy kits worked for making our own needle biopsies. Leica 
already has the protocols that work so part is easy. 


Sent from Yahoo Mail for iPad


On Thursday, March 23, 2023, 3:05 PM, Normington, Lacy via Histonet 
 wrote:

We have purchased several disposable core biopsy instruments of varying gauges 
and take samples of large resection specimens. We also use the same grossing 
tools to take small samples of GI, endo, ecc, ect.

Lacy
 

-Original Message-
From: Cooper, Brian via Histonet  
Sent: Thursday, March 23, 2023 1:54 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Tissue Processor Schedule Validations

WARNING: This email appears to have originated outside of the UW Health email 
system.
DO NOT CLICK on links or attachments unless you recognize the sender and know 
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Good afternoon Histonet,

We're going to be validating a new tissue processor (Peloris 3) in the coming 
months, and I'm curious how people have validated small tissue processing 
protocols (GI bx's, liver/renal needle cores).  Larger tissues are much easier 
to do because we can readily gross duplicate sections. Obviously we can't adopt 
this approach for smaller samples because they're entirely submitted.  I have a 
game plan in mind, but would love some additional input! How'd you do it?

Thanks,

Brian D. Cooper, HT (ASCP)CMQIHCCM| Histology Supervisor Department of 
Pathology and Laboratory Medicine Children's Hospital Los Angeles
4650 Sunset Blvd MS#43- Los Angeles, CA 90027
Ph: 323.361.3357
bcoo...@chla.usc.edu<mailto:bcoo...@chla.usc.edu>

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Re: [Histonet] Tissue Processor Schedule Validations

[Normington, Lacy via Histonet]

We have purchased several disposable core biopsy instruments of varying gauges 
and take samples of large resection specimens. We also use the same grossing 
tools to take small samples of GI, endo, ecc, ect.

Lacy
 

-Original Message-
From: Cooper, Brian via Histonet  
Sent: Thursday, March 23, 2023 1:54 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Tissue Processor Schedule Validations

WARNING: This email appears to have originated outside of the UW Health email 
system.
DO NOT CLICK on links or attachments unless you recognize the sender and know 
the content is safe.

Good afternoon Histonet,

We're going to be validating a new tissue processor (Peloris 3) in the coming 
months, and I'm curious how people have validated small tissue processing 
protocols (GI bx's, liver/renal needle cores).  Larger tissues are much easier 
to do because we can readily gross duplicate sections. Obviously we can't adopt 
this approach for smaller samples because they're entirely submitted.  I have a 
game plan in mind, but would love some additional input! How'd you do it?

Thanks,

Brian D. Cooper, HT (ASCP)CMQIHCCM| Histology Supervisor Department of 
Pathology and Laboratory Medicine Children's Hospital Los Angeles
4650 Sunset Blvd MS#43- Los Angeles, CA 90027
Ph: 323.361.3357
bcoo...@chla.usc.edu<mailto:bcoo...@chla.usc.edu>

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Re: [Histonet] Tissue Processor Schedule Validations

[Whitaker, Bonnie via Histonet]

Put in two cassettes of resection tissue that are cut to mimic biopsy 
sizes/tissue (colon resection for colon bx, liver resection for liver bx, etc.

Bonnie Whitaker
Ohio State University
AP Operations Director

614-293-8418

-Original Message-
From: Cooper, Brian via Histonet  
Sent: Thursday, March 23, 2023 2:54 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Tissue Processor Schedule Validations

Good afternoon Histonet,

We're going to be validating a new tissue processor (Peloris 3) in the coming 
months, and I'm curious how people have validated small tissue processing 
protocols (GI bx's, liver/renal needle cores).  Larger tissues are much easier 
to do because we can readily gross duplicate sections. Obviously we can't adopt 
this approach for smaller samples because they're entirely submitted.  I have a 
game plan in mind, but would love some additional input! How'd you do it?

Thanks,

Brian D. Cooper, HT (ASCP)CMQIHCCM| Histology Supervisor Department of 
Pathology and Laboratory Medicine Children's Hospital Los Angeles
4650 Sunset Blvd MS#43- Los Angeles, CA 90027
Ph: 323.361.3357
bcoo...@chla.usc.edu<mailto:bcoo...@chla.usc.edu>

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the sole use of the intended recipient(s) and may contain confidential or 
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[Histonet] Tissue Processor Schedule Validations

[Cooper, Brian via Histonet]

Good afternoon Histonet,

We're going to be validating a new tissue processor (Peloris 3) in the coming 
months, and I'm curious how people have validated small tissue processing 
protocols (GI bx's, liver/renal needle cores).  Larger tissues are much easier 
to do because we can readily gross duplicate sections. Obviously we can't adopt 
this approach for smaller samples because they're entirely submitted.  I have a 
game plan in mind, but would love some additional input! How'd you do it?

Thanks,

Brian D. Cooper, HT (ASCP)CMQIHCCM| Histology Supervisor
Department of Pathology and Laboratory Medicine
Children's Hospital Los Angeles
4650 Sunset Blvd MS#43- Los Angeles, CA 90027
Ph: 323.361.3357
bcoo...@chla.usc.edu

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Re: [Histonet] Tissue processor advice

[Bacon, Charles via Histonet]

Hi Colleen,

We have all VIPs here at BMC but I have used the Leica in the past at a 
previous position. We currently have 3 - E300s, 3 - VIP 5s, and 2 - VIP 6s. The 
VIPs are great and really only need preventive stuff like O rings and 
lubrication. A service company for us Avantik takes great care of all of that. 
You can get refurbished VIPs at great prices and they will last!

Chuck Bacon, HTL(ASCP)CM 
Supervisor Histology
Baystate Medical Center
361 Whitney Ave., Holyoke, MA 01040
Telephone: 413-322-4786  Fax: 413-322-4790
charles.ba...@baystatehealth.org

-Original Message-
From: Colleen Forster  
Sent: Wednesday, September 16, 2020 1:17 PM
To: histonet-request 
Subject: [Histonet] Tissue processor advice

HEllo Histoneters,

I am looking at replacing my VIP2000, porr girl finally quit on me.

The two processors I am looking at:

Leica ASP300S
Sakura VIP5

Any of you out there who have used either of these or both, can you give me
pros , cons, yes, noI am just looking for experiences those who have
used them can share.

Thank you in advance.

Colleen Forster HT(ASCP)QIHC
BLS Histology and IHC Laboratory
Jackson Hall, Room 2-155
612-626-1930



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[Histonet] Tissue processor Pros/Cons

[Dessasau,, Evan D. via Histonet]

I Collen, I have only used a VIP 5 and love it.  They are workhorses when well 
cared for.  We recently acquired the VIP 6 AI to back up the remaining years of 
our VIP 5.  The last time our VIP 5 had a PM it was down for 4 days which 
included a weekend and we only have one processor.  The VIP 6 has many up 
grades for Solution Management.  We have not had it long enough to give 
feedback.  Sakura only makes the VIP 6 now so you may want to consider the VIP 
6 instead of the VIP 5.
Thank you,
E-van
Yerkes Research Center of
Emory University
Rm. 2122
(404) 727-7744 (on campus 7-7744) Lab
edde...@emory.edu



-Original Message-
From: Colleen Forster via Histonet 
Sent: Wednesday, September 16, 2020 1:17 PM
To: histonet-request 
Subject: [External] [Histonet] Tissue processor advice

HEllo Histoneters,

I am looking at replacing my VIP2000, porr girl finally quit on me.

The two processors I am looking at:

Leica ASP300S
Sakura VIP5

Any of you out there who have used either of these or both, can you give me 
pros , cons, yes, noI am just looking for experiences those who have used 
them can share.

Thank you in advance.

Colleen Forster HT(ASCP)QIHC
BLS Histology and IHC Laboratory
Jackson Hall, Room 2-155
612-626-1930
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[Histonet] Tissue processor advice

[Colleen Forster via Histonet]

HEllo Histoneters,

I am looking at replacing my VIP2000, porr girl finally quit on me.

The two processors I am looking at:

Leica ASP300S
Sakura VIP5

Any of you out there who have used either of these or both, can you give me
pros , cons, yes, noI am just looking for experiences those who have
used them can share.

Thank you in advance.

Colleen Forster HT(ASCP)QIHC
BLS Histology and IHC Laboratory
Jackson Hall, Room 2-155
612-626-1930
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Re: [Histonet] Tissue processor validation

[Simmons, Christopher via Histonet]

Get your gross lab to provide you with samples representative of what you 
normally process and the same for tissue you use for internally procured 
special stain controls (kidney, lung, etc)
Process as usual and cut and perform specials
If they work: done.

Get Outlook for iOS<https://aka.ms/o0ukef>

From: Martha Ward-Pathology via Histonet 
Sent: Wednesday, September 2, 2020 3:51:42 PM
To: histonet@lists.utsouthwestern.edu 
Subject: [Histonet] Tissue processor validation

I am posting this question for my colleague in the Histology lab.   She is 
working on a plan for a new tissue processor validation, including special 
stains, and would like to get input from anyone that has already gone through 
it.This is the first time anyone here has ever been involved in validating 
a new processor.   Current ones are 25+ years.


Any help or advice would be appreciated.   Thanks in advance!

Martha Ward, MT (ASCP) QIHC
Wake Forest Baptist Health
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[Histonet] Tissue processor validation

[Martha Ward-Pathology via Histonet]

I am posting this question for my colleague in the Histology lab.   She is 
working on a plan for a new tissue processor validation, including special 
stains, and would like to get input from anyone that has already gone through 
it.This is the first time anyone here has ever been involved in validating 
a new processor.   Current ones are 25+ years.


Any help or advice would be appreciated.   Thanks in advance!

Martha Ward, MT (ASCP) QIHC
Wake Forest Baptist Health
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Re: [Histonet] Tissue processor errors, failures and what to do

[E. Wayne Johnson via Histonet]

Automation is a wonderful thing but it is only a replacement for what people 
used to do by hand.
We have incubators that can be set to 60C and we have a Rube Goldberg-ized 
microwave oven with
a thermal controller and relays (and the not-to-be-forgotten flyback diode) and
a K-type thermal probe covered with aluminum foil (dont try this at home, kids)
so we are ready for any incident or mishap with our cantankerous VIP5.

There is no such thing as a convenient time for it to quit but lots of times 
the trouble
is that it was time to change the solutions and nobody did it even though
anybody could have done it.

If there is nothing we can do to get the VIP5 going again pronto
we finish the processing by hand.
there are repair manuals for some of those machines available online
or you can call the repairman.  If you are out in Bufooee somewhere the repair
manual and the secret repair modes on the machine could be the difference 
between joy
and the slough of despond.

E. Wayne Johnson DVM
Enable AgTech
Beijing

Patpxs via Histonet wrote:

Hi Garrey,

The answer is “it depends”.   What you do when a processor fails depends on the 
failure point.  If the tissue is still in dehydrant it gets treated differently 
than if it fails in the intermediate solvent.

Paula

Sent from my iPhone


On Jul 4, 2020, at 10:08 AM, Garrey Faller via Histonet 
 wrote:

Happy 4th to all.
Does anyone have a procedure on what to do when a tissue processor fails or 
alarms.  I want to learn more about the science behind tissue processing so I 
know what to do when the machine fails. This happened to a friend recently and 
I want to prevent my tissues/biopsies from being ruined.
Thanks.
Garrey

Sent from my iPhone
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Re: [Histonet] Tissue processor errors, failures and what to do

[Patpxs via Histonet]

Hi Garrey,

The answer is “it depends”.   What you do when a processor fails depends on the 
failure point.  If the tissue is still in dehydrant it gets treated differently 
than if it fails in the intermediate solvent.  

Paula

Sent from my iPhone

> On Jul 4, 2020, at 10:08 AM, Garrey Faller via Histonet 
>  wrote:
> 
> Happy 4th to all.
> Does anyone have a procedure on what to do when a tissue processor fails or 
> alarms.  I want to learn more about the science behind tissue processing so I 
> know what to do when the machine fails. This happened to a friend recently 
> and I want to prevent my tissues/biopsies from being ruined.
> Thanks.
> Garrey
> 
> Sent from my iPhone
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[Histonet] Tissue processor errors, failures and what to do

[Garrey Faller via Histonet]

Happy 4th to all.
Does anyone have a procedure on what to do when a tissue processor fails or 
alarms.  I want to learn more about the science behind tissue processing so I 
know what to do when the machine fails. This happened to a friend recently and 
I want to prevent my tissues/biopsies from being ruined.
Thanks.
Garrey

Sent from my iPhone
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[Histonet] Tissue Processor Validation

[Ashwin Ashwin via Histonet]

Hello Everyone,
If we change a processing protocol and are in need to recalibrate our tissue 
processors, is it required to perform concordance study between the old 
protocol and the new?
Please let me know
Thanks

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Re: [Histonet] Tissue Processor

[Terri Braud via Histonet]

After 40 years of using primarily Miles/Tissue Tek, we recently purchased 2 
Leica ASP 6025.  With the exception of a small glitch with faulty sensors, and 
a learning curve to get used to them, I would highly recommend them.  We had 
replaced an ancient K series(with the magnet) and a VIP 5.
The quality and speed of the processing from the Leica's is incredible.  We had 
great tech support in setting up and validating processing protocols.  We ended 
up using several of their pre-set protocols which worked great, especially for 
the processing of large tissues.  I was expecting better processing than our 
ancient Tissue Tek, but the idea that it outperformed the VIP 5 surprised me.  
Reagents are a snap to exchange and the software/screen is very user friendly.
I hope this helps, Terri

Terri L. Braud, HT(ASCP)
Anatomic Pathology Supervisor
Laboratory
Holy Redeemer Hospital
1648 Huntingdon Pike
Meadowbrook, PA 19046
ph: 215-938-3689
fax: 215-938-3874
Care, Comfort, and Heal
Today's Topics:

   1. tissue processor (Lisa Brenner)
--
Message: 1
From: "Lisa Brenner" <li...@hollandhospital.org>
Subject: [Histonet] tissue processor
Hello,
   We are in the market for a new vacuum infiltrating tissue processor. Our old 
VIP has been a work horse but needs replacing. What is everyone using? What 
works well? What's new?
Lisa Brenner
**


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Re: [Histonet] tissue processor

[Cooper, Brian via Histonet]

We absolutely love our Leica Peloris II.

Thanks, 

Brian D. Cooper, HT (ASCP)CM | Histology Supervisor 
Department of Pathology and Laboratory Medicine
Children's Hospital Los Angeles 
4650 Sunset Blvd MS#43- Los Angeles, CA 90027 
bcoo...@chla.usc.edu 

-Original Message-
From: Lisa Brenner via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Friday, February 02, 2018 10:36 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] tissue processor (EXTERNAL EMAIL)

Hello,

   We are in the market for a new vacuum infiltrating tissue processor. Our old 
VIP has been a work horse but needs replacing. What is everyone using? What 
works well? What's new?

Lisa Brenner


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[Histonet] tissue processor

[Lisa Brenner via Histonet]

Hello,

   We are in the market for a new vacuum infiltrating tissue processor. Our old 
VIP has been a work horse but needs replacing. What is everyone using? What 
works well? What's new?

Lisa Brenner


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[Histonet] Tissue Processor question

[Terri Braud via Histonet]

Hi fellow Histonetters-
I'm looking for any feedback from working labs using Thermo's Excelsior 
Processor.  If you can help, can you please also include the age and usage on 
your instrument?
Thanks, Terri

Terri L. Braud, HT(ASCP)
Anatomic Pathology Supervisor
Laboratory
Holy Redeemer Hospital
1648 Huntingdon Pike
Meadowbrook, PA 19046
ph: 215-938-3689
fax: 215-938-3874

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[Histonet] Tissue processor advice for a small start-up

[Caroline Miller via Histonet]

Hi All,

Our company has reached the size where we can justify buying our own
processor! Yippee!!

However that also leads me to the issue of which one to buy :)

We will have very small batches of blocks to process, approx 10 per process
maybe twice per week, but we need the machine to be very responsive to
different processes - we have a microscope that sections and images at the
same time, therefore we do the staining prior to processing, and have to
tweak the protocols to keep enough contrast int the tissue by the end.

In short:
- low volume
- easy to use and change protocols
- good support for servicing and fixing should things go wrong.
- No formalin will touch the machine, and we will want to change out
solvents from the standard alcohols and xylenes (therefore I need a machine
who's warranty would not be affected by this.
- I would love to get this all in one package and buy the service contract
at the same time as the machine, preferably with the same company.
- If it helps with the cost, then I also want to buy a paraffin microtome :)

We are in San Francisco.

Commercial replies are totally acceptable here, but please do not reply all.

Thanks, as ever, histonet!

yours,
mills

-- 
Caroline Miller (mills)
Director of Histology
3Scan.com
415 2187297
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[Histonet] Tissue Processor Reviews

[ODea, Elise via Histonet]

Thank you all for your opinions and experiences of Excelsior and Sakura!

Elise



















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RE: [Histonet] Tissue Processor Validation Procedure

[Jamal]

Hi Allison
I hope the attached can help you



Best Regards,


Jamal M. Al Rowaihi Anatomic Pathology Supervisor   | Al Borg
Medical Laboratories |  Mobile +966 503629832|
j.rowa...@alborglaboratories.com 
Palestine St, Al Rajhi Building, P.O. Box 52817, Jeddah 21573, KSA|
Phone: +966 12 670 0099   | Fax: +966 12 676 4984 |
www.alborglaboratories.com


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Scott,
Allison D
Sent: Thursday, January 29, 2015 10:44 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Tissue Processor Validation Procedure

Hello to all in Histoland.  Does anyone have a tissue processor validation
procedure that they would be willing to share?  Any help in this would be
greatly appreciated.

Allison Scott HT(ASCP)
Histology Supervisor
LBJ Hospital
713-566-5287(Lab)
713-566-2148(Office)

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[Histonet] Tissue Processor Validation Procedure

[Johnson, Carole]

Hello Allison,

When we purchased a new processor, I chose tissue specimens representative of 
what we normally see in our lab (surgical/biopsy and necropsy tissues) from 
cases which had been signed out within the previous 48-72 hours. I then ran 
parallel samples on both processors (same protocols, run the same day, fresh 
reagents in both processors) and then set up a grading scale for the 
pathologists to do a blind comparison. After that, I did a (brief) statistical 
analysis to determine if there was any significant difference between slides 
from the two processors. For IHC (we are a small lab with a short IHC menu 
currently), I ran parallel Vimentin and Multicytokeratin on the same blocks as 
used for the HE and did a comparison just as for the HE slides. I presented 
the final data to my chief pathologist who then signed off on the validation. I 
keep a copy of the validation with my equipment records. I hope this helps.

Best regards,
Carole

Carole Johnson
Carole Johnson, HT(ASCP)cm
New Mexico Department of Agriculture
Veterinary Diagnostic Services
505.383.9299

To understand is to stand under, which is to look up, which is a good way to 
understand




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[Histonet] Tissue Processor Validation Procedure

[Scott, Allison D]

Hello to all in Histoland.  Does anyone have a tissue processor validation 
procedure that they would be willing to share?  Any help in this would be 
greatly appreciated.

Allison Scott HT(ASCP)
Histology Supervisor
LBJ Hospital
713-566-5287(Lab)
713-566-2148(Office)

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[Histonet] Tissue Processor

[Patti McDavid]

We have used VIP in the past and it was adequate.  We use the
Leica Peloris as our main processor and love it.

Patti L. McDavid
Laboratory Manager
4500 Thirteenth Street
P.O. Box 1810
Gulfport, MS  39502-1810
Phone 228-575-2340
Fax 228-865-3325
pmcda...@mhg.commailto:pmcda...@mhg.com




[http://www.gulfportmemorial.com/images/14MH81-BESTE_SIG-RANKED-A-BESTD2.GIF]
http://health.usnews.com/best-hospitals/area/ms

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RE: [Histonet] Tissue Processor Validation Procedure

[Jamal]

Dear colleague
I hope the attached help you



Best Regards,


Jamal M. Al Rowaihi Anatomic Pathology Supervisor   | Al Borg
Medical Laboratories |  Mobile +966 503629832|
j.rowa...@alborglaboratories.com 
Palestine St, Al Rajhi Building, P.O. Box 52817, Jeddah 21573, KSA|
Phone: +966 12 670 0099   | Fax: +966 12 676 4984 |
www.alborglaboratories.com


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Adesupo,
Adesuyi (Banjo)
Sent: Thursday, November 06, 2014 11:49 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Tissue Processor Validation Procedure

Hi,
   I am wondering if you guys in histoland will like to share your Tissue
Processor Validation Procedure with me.

Thanks,
Adesuyi
==
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[Histonet] Tissue Processor Validation Procedure

[Adesupo, Adesuyi (Banjo)]

Hi,
   I am wondering if you guys in histoland will like to share your Tissue 
Processor Validation Procedure with me.

Thanks,
Adesuyi
==
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[Histonet] Tissue Processor Location in the Lab

[Wheelock, Timothy R.]

Hi  Everyone:

Even though many tissue processors are closed systems with fume filters, do 
people try to put them next to fume hoods or under some ventilation duct to add 
an extra layer of protection from solvent fumes?

Thanks,

Tim Wheelock
Harvard Brain Tissue Resource Center
McLean Hospital
Belmont, MA


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RE: [Histonet] Tissue Processor Validation:

[Jamal]

Hi Jb
The attached is my validation form you can refer to it.


Best Regards,


Jamal M. Al Rowaihi Anatomic Pathology Supervisor   | Al Borg
Medical Laboratories |  Mobile +966 503629832|
j.rowa...@alborglaboratories.com 
Palestine St, Al Rajhi Building, P.O. Box 52817, Jeddah 21573, KSA|
Phone: +966 12 670 0099   | Fax: +966 12 676 4984 |
www.alborglaboratories.com


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jb
Sent: Friday, September 05, 2014 9:11 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Tissue Processor Validation:

Does anyone have a tissue processor validation form that they are willing to
share?

Thank you. 

Sent from my iPhone
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Re: [Histonet] Tissue Processor Validation:

[Mike Andrews]

It appears that the list software strips attachments. You may want to put it on 
a website and post the URL.

Mike Andrews, W5EGO
WWME Oklahoma area executive team

 On Sep 6, 2014, at 4:34 AM, Jamal j.rowa...@alborglaboratories.com wrote:
 
 Hi Jb
 The attached is my validation form you can refer to it.
 
 
 Best Regards,
 
 
 Jamal M. Al RowaihiAnatomic Pathology Supervisor   | Al Borg
 Medical Laboratories |  Mobile +966 503629832|
 j.rowa...@alborglaboratories.com 
 Palestine St, Al Rajhi Building, P.O. Box 52817, Jeddah 21573, KSA|
 Phone: +966 12 670 0099  | Fax: +966 12 676 4984 |
 www.alborglaboratories.com
 
 
 -Original Message-
 From: histonet-boun...@lists.utsouthwestern.edu
 [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jb
 Sent: Friday, September 05, 2014 9:11 PM
 To: histonet@lists.utsouthwestern.edu
 Subject: [Histonet] Tissue Processor Validation:
 
 Does anyone have a tissue processor validation form that they are willing to
 share?
 
 Thank you. 
 
 Sent from my iPhone
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[Histonet] Tissue Processor Validation:

[Jb]

Does anyone have a tissue processor validation form that they are willing to 
share?

Thank you. 

Sent from my iPhone
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[Histonet] tissue processor regulations

[Ronda Mire]

Hello Histonet,

I recently started work in a research lab after working in a clinical setting 
for 20 years.  The lab that I was at had all the tissue processors in a 
separate negative pressure room. The lab that I work at now has the processors 
out in the main work area.  I noticed the fumes instantly and now several of 
the employees have complained of problems with the fumes.  I want to move all 
the processors away from the cutting areas into a separate room.  Any 
suggestions or comments that I could add to my justification would be helpful.  
Thanks

Ronda Mire
Laboratory Manager
CVPath Institute
Gaithersburg, MD
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[Histonet] Tissue Processor

[Vickroy, Jim]

Wondered if anyone had any thoughts or information on the comparison of two 
tissue processors that we are considering.   The two instruments are the Leica 
ASP6025 and the Thermofisher Excelsior ES.  We originally also considered 
another VIP.  The Leica ASP6025 and the Thermofisher unit have a lot of 
similarities but there are some definite differences including price.  I wish 
there was a Consumer Reports study of tissue processors because making the 
correct choice is getting harder and harder.   Our tissue processors run every 
day and I definitely want an instrument to be able to take the wear and tear of 
every day usage.  Obviously the VIP 6 has a family of processors that have 
lasted for years but I'm not sure that they have kept up with the new 
technology and bells and whistles that make usage easier and more 
cost-effective.

If you have any experience with one of these processors please let me know, 
good or bad.  I have been in histology for over 35 years and this is the first 
time I have felt like I'm buying a car.
There are so many rumors out there such as:


1.You have to use proprietary reagents with this processor because the 
easy fill only works with their bottles. (recall)

2.   One instrument will not last as long as the other because of materials 
in the design.

3.   Service will be a problem with one company and not the other, because 
of who is trained to service the instrument.

4.   Etc, Etc, Etc.

I would be forever grateful for any additional information you could give me.  
Thanks



James Vickroy BS, HT(ASCP)

Surgical  and Autopsy Pathology Technical Supervisor
Memorial Medical Center
217-788-4046



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RE: [Histonet] Tissue Processor Validation

[tahseen]

Dear All
Would any one of you out there,want to share template of Tissue 
Processor validation forms?

Thanks
Muhammad Tahseen
Histolog Supervisor
SKMCHRC Lahore
Pakistan

On 2013-08-28 19:10, Horn, Hazel V wrote:

We did the same as Beth.

Hazel Horn
ySupervisor of Histolog/Autopsy/Transcription
Anatomic Pathology
Arkansas Children's Hospital
1 Children's Way | Slot 820| Little Rock, AR 72202
501.364.4240 direct | 501.364.1241 fax
hor...@archildrens.org
archildrens.org






-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Beth
Brinegar
Sent: Wednesday, August 28, 2013 8:40 AM
To: Rathborne, Toni
Cc: histonet@lists.utsouthwestern.edu; dianar...@aol.com
Subject: Re: [Histonet] Tissue Processor Validation

Yes, we do a few validation runs. Nothing too extreme, however we
gross some extra patient tissue and do a small run, then increase the
amount of blocks processed until we are ready to run cases for 
patient

diagnosis. It really only takes a week or two. After those blocks are
cut, then we compare them to the previous processor results and make
changes if neccessary.

Beth Brinegar HTL(ASCP)
Anatomic Pathology Supervisor
Mercy Medical Center
Cedar Rapids, IA 52403


On Wed, Aug 28, 2013 at 8:27 AM, Rathborne, Toni 
trathbo...@somerset-healthcare.com wrote:


How do you validate IHC  FISH with a new processor?

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu [mailto:
histonet-boun...@lists.utsouthwestern.edu] On Behalf Of joelle 
weaver

Sent: Wednesday, August 28, 2013 9:23 AM
To: dianar...@aol.com; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Tissue Processor Validation

Yes definitely, 5 programs, 25-50 sister, serial sections on tissue
that represents patient samples.




Joelle Weaver MAOM, HTL (ASCP) QIHC

 From: dianar...@aol.com
 Date: Tue, 27 Aug 2013 22:07:19 -0400
 To: histonet@lists.utsouthwestern.edu
 Subject: [Histonet] Tissue Processor Validation

 I am interested in knowing how many of you out there Validate your
 Tissue Processors before you put them into use and how extreme you 
go.


 Diana Ripley
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[Histonet] Tissue Processor

[Sue]

We always validated our processor but not formally.  CAP wants a more formal 

approach with procedures. 



What we did in the past was run placenta and colon and reviewed stained slides. 

We may have to be a little more critical and run various types of slides and 
programs. 



Susan T. Paturzo HT (ASCP) 




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Re: [Histonet] Tissue Processor Validation

[Beth Brinegar]

Yes, we do a few validation runs. Nothing too extreme, however we gross
some extra patient tissue and do a small run, then increase the amount of
blocks processed until we are ready to run cases for patient diagnosis. It
really only takes a week or two. After those blocks are cut, then we
compare them to the previous processor results and make changes if
neccessary.

Beth Brinegar HTL(ASCP)
Anatomic Pathology Supervisor
Mercy Medical Center
Cedar Rapids, IA 52403


On Wed, Aug 28, 2013 at 8:27 AM, Rathborne, Toni 
trathbo...@somerset-healthcare.com wrote:

 How do you validate IHC  FISH with a new processor?

 -Original Message-
 From: histonet-boun...@lists.utsouthwestern.edu [mailto:
 histonet-boun...@lists.utsouthwestern.edu] On Behalf Of joelle weaver
 Sent: Wednesday, August 28, 2013 9:23 AM
 To: dianar...@aol.com; histonet@lists.utsouthwestern.edu
 Subject: RE: [Histonet] Tissue Processor Validation

 Yes definitely, 5 programs, 25-50 sister, serial sections on tissue that
 represents patient samples.




 Joelle Weaver MAOM, HTL (ASCP) QIHC

  From: dianar...@aol.com
  Date: Tue, 27 Aug 2013 22:07:19 -0400
  To: histonet@lists.utsouthwestern.edu
  Subject: [Histonet] Tissue Processor Validation
 
  I am interested in knowing how many of you out there Validate your
  Tissue Processors before you put them into use and how extreme you go.
 
  Diana Ripley
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RE: [Histonet] Tissue Processor Validation

[joelle weaver]

 On Wed, Aug 28, 2013 at 8:27 AM, Rathborne, Toni  
trathbo...@somerset-healthcare.com wrote: 
  How do you validate IHC  FISH with a new processor?
 
You are going to have to revalidate IHC and FISH with new processing. How much 
might depend on if you are significantly changing instrumentation/technology, 
or just getting a new instrument of the same type. I usually don't do a full, 
as new assay validation, but the amount needed just to show that the processing 
change has not affected the test results. If there are issues that appear with 
that, you might have to do a complete revalidation. 




Joelle Weaver MAOM, HTL (ASCP) QIHC
 
 From: hor...@archildrens.org
 To: bbrinegar...@gmail.com; trathbo...@somerset-healthcare.com
 Date: Wed, 28 Aug 2013 09:10:39 -0500
 Subject: RE: [Histonet] Tissue Processor Validation
 CC: histonet@lists.utsouthwestern.edu; dianar...@aol.com
 
 We did the same as Beth.
 
 Hazel Horn
 Supervisor of Histology/Autopsy/Transcription
 Anatomic Pathology
 Arkansas Children's Hospital
 1 Children's Way | Slot 820| Little Rock, AR 72202
 501.364.4240 direct | 501.364.1241 fax
 hor...@archildrens.org
 archildrens.org
 
 
 
 
 
 
 -Original Message-
 From: histonet-boun...@lists.utsouthwestern.edu 
 [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Beth Brinegar
 Sent: Wednesday, August 28, 2013 8:40 AM
 To: Rathborne, Toni
 Cc: histonet@lists.utsouthwestern.edu; dianar...@aol.com
 Subject: Re: [Histonet] Tissue Processor Validation
 
 Yes, we do a few validation runs. Nothing too extreme, however we gross some 
 extra patient tissue and do a small run, then increase the amount of blocks 
 processed until we are ready to run cases for patient diagnosis. It really 
 only takes a week or two. After those blocks are cut, then we compare them to 
 the previous processor results and make changes if neccessary.
 
 Beth Brinegar HTL(ASCP)
 Anatomic Pathology Supervisor
 Mercy Medical Center
 Cedar Rapids, IA 52403
 
 
 On Wed, Aug 28, 2013 at 8:27 AM, Rathborne, Toni  
 trathbo...@somerset-healthcare.com wrote:
 
  How do you validate IHC  FISH with a new processor?
 
  -Original Message-
  From: histonet-boun...@lists.utsouthwestern.edu [mailto:
  histonet-boun...@lists.utsouthwestern.edu] On Behalf Of joelle weaver
  Sent: Wednesday, August 28, 2013 9:23 AM
  To: dianar...@aol.com; histonet@lists.utsouthwestern.edu
  Subject: RE: [Histonet] Tissue Processor Validation
 
  Yes definitely, 5 programs, 25-50 sister, serial sections on tissue 
  that represents patient samples.
 
 
 
 
  Joelle Weaver MAOM, HTL (ASCP) QIHC
 
   From: dianar...@aol.com
   Date: Tue, 27 Aug 2013 22:07:19 -0400
   To: histonet@lists.utsouthwestern.edu
   Subject: [Histonet] Tissue Processor Validation
  
   I am interested in knowing how many of you out there Validate your 
   Tissue Processors before you put them into use and how extreme you go.
  
   Diana Ripley
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[Histonet] Tissue Processor Validation

[DianaRip1]

I am interested in knowing how many of you out there Validate your Tissue  
Processors before you put them into use and how extreme you go.
 
Diana Ripley
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[Histonet] tissue processor remote alarm systems

[Morken, Timothy]

I'd like to hear from labs that use a remote messaging system for their VIP5 
tissue processors that are left unattended overnight. One I saw on old histonet 
posts is Sensaphone.

I am wondering what kind of information you can get from the system. Does it 
just say there is a problem but no details?

Can you connect several processors to one sensing unit? Can it tell the 
difference between the units (ie, processor 1 vs processor 4?).

Thanks for any info!

Tim Morken
Supervisor, Electron Microscopy/Neuromuscular Special Studies
Department of Pathology
UC San Francisco Medical Center

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RE: [Histonet] tissue processor remote alarm systems

[Dessoye, Michael J]

I have a Sensaphone unit.  It can handle four different instruments.
When it calls it will give you the phone number it's calling from, the
sensor (instrument) number, and the 'alert condition' (there are
several...for example 1 is power failure, etc.)  Any other questions
about it I'm happy to help.

Michael J. Dessoye, M.S. | Histology Supervisor | Wilkes-Barre General
Hospital | An Affiliate of Commonwealth Health |
mjdess...@commonwealthhealth.net | 575 N. River Street | Wilkes Barre,
PA 18764 | Tel: 570-552-1432 | Fax: 570-552-1526 
 

-Original Message-
From: Morken, Timothy [mailto:timothy.mor...@ucsfmedctr.org] 
Sent: Thursday, February 14, 2013 11:01 AM
To: Histonet
Subject: [Histonet] tissue processor remote alarm systems

I'd like to hear from labs that use a remote messaging system for their
VIP5 tissue processors that are left unattended overnight. One I saw on
old histonet posts is Sensaphone.

I am wondering what kind of information you can get from the system.
Does it just say there is a problem but no details?

Can you connect several processors to one sensing unit? Can it tell the
difference between the units (ie, processor 1 vs processor 4?).

Thanks for any info!

Tim Morken
Supervisor, Electron Microscopy/Neuromuscular Special Studies Department
of Pathology UC San Francisco Medical Center


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[Histonet] TISSUE PROCESSOR FOLLOW-UP QUESTION

[Tim Wheelock]

Hi All:

First, thank you for all your feedback on the processors.

I site-visited a VIP6 at a local hospital
From my understanding the VIP6 can rotate absolute alcohol and xylene 
(using the bulk reservoirs), as well as the paraffin stations, but it 
cannot rotate other concentrations of alcohol based on hydrometer readings.

Am I correct in this appraisal?

Tim Wheelock
Neuropathology Laboratory
Harvard Brain Bank
McLean Hospital
Belmont, MA

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RE: [Histonet] TISSUE PROCESSOR FOLLOW-UP QUESTION

[Cynthia Pyse]

Yes you are.
Cindy

Cindy Pyse, CLT, HT (ASCP)
Laboratory Manager
X-Cell Laboratories
20 Northpointe Parkway Suite 100
Amherst, NY 14228
716-250-9235 etx. 232
e-mail cp...@x-celllab.com

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Tim Wheelock
Sent: Friday, December 07, 2012 12:44 PM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] TISSUE PROCESSOR FOLLOW-UP QUESTION

Hi All:

First, thank you for all your feedback on the processors.

I site-visited a VIP6 at a local hospital  From my understanding the VIP6
can rotate absolute alcohol and xylene (using the bulk reservoirs), as well
as the paraffin stations, but it cannot rotate other concentrations of
alcohol based on hydrometer readings.
Am I correct in this appraisal?

Tim Wheelock
Neuropathology Laboratory
Harvard Brain Bank
McLean Hospital
Belmont, MA

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[Histonet] Tissue Processor

[Heckford, Karen - SMMC-SF]

I am going to need to purchase a new tissue processor mine keeps breaking down. 
 What tissue processor would you buy and why?  I would greatly appreciate the 
help.

Cheers,

Karen Heckford HT ASCP CE
Lead Histology Technician
St. Mary's Medical Center
450 Stanyan St.
San Francisco, Ca. 94117
415-668-1000 ext. 6167
karen.heckf...@dignityhealth.org

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Re: [Histonet] Tissue Processor

[Rene J Buesa]

Sakura
René J.



From: Heckford, Karen - SMMC-SF karen.heckf...@dignityhealth.org
To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu 
Sent: Wednesday, August 8, 2012 8:11 AM
Subject: [Histonet] Tissue Processor

I am going to need to purchase a new tissue processor mine keeps breaking 
down.  What tissue processor would you buy and why?  I would greatly appreciate 
the help.

Cheers,

Karen Heckford HT ASCP CE
Lead Histology Technician
St. Mary's Medical Center
450 Stanyan St.
San Francisco, Ca. 94117
415-668-1000 ext. 6167
karen.heckf...@dignityhealth.org
                                                                                
  Caution:  This email message, including all content and attachments, is 
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information contained in this email message is intended only for the use of the 
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Re: [Histonet] Tissue Processor

[Pam Marcum]

We have swithched from the VIP Sakura, older models, to the Thermo Excelsior.  
We are currently buying our 3rd and 4th units for the Histology Lab.  They have 
features we really liked and cut my our exposure to formalin and xylene by 
almost 90% due to the way the system changes solutions.  Due to the very gently 
agitation used during processing we were also able to cut some time off our 
programs for overnight and short cycle biopsy runs.  



We stil use a Leica ASP 300 for bone marrows only as it is what need due to 
reagent requirements for xylene sub on the last two stations.  



If you have any questions let me know. 


Pam Marcum 

UAMS 



- Original Message -
From: Karen - SMMC-SF Heckford karen.heckf...@dignityhealth.org 
To: histonet@lists.utsouthwestern.edu 
Sent: Wednesday, August 8, 2012 7:11:13 AM 
Subject: [Histonet] Tissue Processor 

I am going to need to purchase a new tissue processor mine keeps breaking down. 
 What tissue processor would you buy and why?  I would greatly appreciate the 
help. 

Cheers, 

Karen Heckford HT ASCP CE 
Lead Histology Technician 
St. Mary's Medical Center 
450 Stanyan St. 
San Francisco, Ca. 94117 
415-668-1000 ext. 6167 
karen.heckf...@dignityhealth.org 
                                                                                
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[Histonet] Tissue Processor

[Gauch, Vicki]

Hi everyone,
I was wondering if anyone had any input on the Logos tissue processor by 
Milestone.  Is anyone currently using it and what are the pros and cons, etc. . 
 Any information would be greatly appreciated.

Thanks,
Vicki Gauch
AMCH
Albany, NY



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[Histonet] Tissue Processor

[Patricia Bourassa]

Hello!

We are looking to purchase a used tissue processor for a small biotech.  We
will be working with calcified bone so we will only use the machine for
dehydration and will infiltrate with methacrylate by hand.

I'm interested in suggestions.  It's been a while since I've done histology
and I'd like to know what machines/ vendors folks would recommend.

Thanks for your help!  Patti

-- 
*Patti Bourassa
Senior Scientist
Karos Pharmaceuticals
(203) 535-0540, ext 207*
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[Histonet] Tissue Processor

[Lori White]

Hi Mike,
We have both the VIP6 and TEC5 and are extremely happy with them.  What you
spend up front, you will save in repair costs, downtime and frustration.
Lori


--

Message: 8
Date: Tue, 04 Oct 2011 11:56:03 -0400
From: Mike Tighe mti...@trudeauinstitute.org
Subject: [Histonet] Tissue processor
To: histonet@lists.utsouthwestern.edu
Message-ID: 4e8af3e6.26e4.00e...@trudeauinstitute.org
Content-Type: text/plain; charset=US-ASCII

I am looking to purchase a Processor and embedding center. I have received a
quote for VIP6 and a TEC5 but they seem  to be a bit out of our price range.
Does anyone have another Processor/embedding center that they found to be
reliable?

Thanks
Mike












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RE: Histonet Digest, Vol 95, Issue 19 [Histonet] Tissue processor

[Steve McClain]

Mike,
If your budget is tight, consider used Sakura VIP processor and
Tissue-Tek embedding center.

One superb vendor is Avantik , formerly Bellair in NJ. 800-783-9424
973-912-8900
They have earned my complete trust-I am reluctant to have equipment in
my  lab unless Bellair can maintain it.
Every piece of equipment I have bought from them and serviced by them
has been solid.

A good used embedding center and processor can be serviceable for 10
years or more, yet inexpensive.

New Tissue processor - Depending on your # of blocks, another
consideration I did not see mentioned is the RVG2 tissue processor sold
by Bellair-Avantik.
We have used it since new with nearly 1100 runs over 3+ years without
problem.
One advantage is the preheater/heat exchanger pre-warming solutions,
allowing for rapid processing, 
e.g., small biopsies in 90 minutes.

Steve A. McClain, MD
631 361 4000
PS I have no financial interst in that company, but I have had great
experience and I am biased toward small companies which deliver great
service.

Message: 8
Date: Tue, 04 Oct 2011 11:56:03 -0400
From: Mike Tighe mti...@trudeauinstitute.org
Subject: [Histonet] Tissue processor
To: histonet@lists.utsouthwestern.edu
Message-ID: 4e8af3e6.26e4.00e...@trudeauinstitute.org
Content-Type: text/plain; charset=US-ASCII

I am looking to purchase a Processor and embedding center. I have
received a
quote for VIP6 and a TEC5 but they seem  to be a bit out of our price
range.
Does anyone have another Processor/embedding center that they found to
be
reliable?

Thanks
Mike

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[Histonet] Tissue processor

[Mike Tighe]

I am looking to purchase a Processor and embedding center. I have received a 
quote for VIP6 and a TEC5 but they seem  to be a bit out of our price range. 
Does anyone have another Processor/embedding center that they found to be 
reliable?

Thanks
Mike





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Re: [Histonet] Tissue processor

[Rene J Buesa]

When you are buying VIP6 or TEC5 you are both buying pot notch technology + 
quality.
For sure you will find others cheaper but of lesser quality.
You should think of this as if buying a new car:
Do you want to buy one cheaper but less reliable, or one that will give you 
years of reliable transportation?
It is the same thing with histology instruments!
René J.

--- On Tue, 10/4/11, Mike Tighe mti...@trudeauinstitute.org wrote:


From: Mike Tighe mti...@trudeauinstitute.org
Subject: [Histonet] Tissue processor
To: histonet@lists.utsouthwestern.edu
Date: Tuesday, October 4, 2011, 11:56 AM


I am looking to purchase a Processor and embedding center. I have received a 
quote for VIP6 and a TEC5 but they seem  to be a bit out of our price range. 
Does anyone have another Processor/embedding center that they found to be 
reliable?

Thanks
Mike





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RE: [Histonet] Tissue processor

[Breeden, Sara]

Although I appreciate individual opinions, do not overlook the Leica
line.  Us old timers may recognize the names Sakura or Thermo (in
its many permutations) more readily, I'd put my money on Leica every
time (and I have - with processor, embedding center, microtome and
autostainer).  They do not let me down.  I have no issues with either
other vendor, but I placed my trust in German engineering.  I'm just
sayin'...

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RE: [Histonet] Tissue processor

[Marcum, Pamela A]

We are just getting our second Excelsior and love them.  It cuts tech time 
changing bottles and paraffin as well as keeping us on track daily for changes 
and rotations.  We have to VIP, that are very old and one just died.  That is 
the one we are replacing with Thermo.  The VIPs are great units just could not 
go the price and reagent exposure with them. We also have Leica ASP300 love but 
skipped it for the same reasons.

Pam Marcum 
UAMS

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sheila Haas
Sent: Tuesday, October 04, 2011 12:08 PM
To: Mike Tighe; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Tissue processor

We purchased a processor this past year and went with the VIP6. We demo'd
several but went with the VIP because of its reliability and quality. We own 
two older models, the oldest over 20 years old, that still run well. We also 
liked the Leica model that was slightly less, but I was able to convince 
administration the VIP was worth the difference.
 
Hope this helps.

Sheila Haas
Laboratory Supervisor
MicroPath Laboratories, Inc.
 

From: Mike Tighe mti...@trudeauinstitute.org
To: histonet@lists.utsouthwestern.edu
Sent: Tuesday, October 4, 2011 11:56 AM
Subject: [Histonet] Tissue processor

I am looking to purchase a Processor and embedding center. I have received a 
quote for VIP6 and a TEC5 but they seem  to be a bit out of our price range. 
Does anyone have another Processor/embedding center that they found to be 
reliable?

Thanks
Mike





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[Histonet] Tissue Processor Reagents

[Katherine Leonard]

Hello histonetters ~

Is there any consensus regarding how often to change processor reagents? I
am using the TBS ATP1 tissue processor and the tissue size ranges from
biopsy to 1.5cm^3.

-- 
Katherine Leonard
Research Associate
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RE: [Histonet] Tissue Processor Reagents

[sgoebel]

I think the standard rule of thumb is 700-800 blocks?

Sarah Goebel, BA, HT(ASCP)
Histotechnologist
Mirna Therapeutics
2150 Woodward Street
Suite 100
Austin, Texas  78744
(512)901-0900 ext. 6912




-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
Katherine Leonard
Sent: Tuesday, January 04, 2011 12:32 PM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] Tissue Processor Reagents

Hello histonetters ~

Is there any consensus regarding how often to change processor reagents?
I
am using the TBS ATP1 tissue processor and the tissue size ranges from
biopsy to 1.5cm^3.

-- 
Katherine Leonard
Research Associate
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[Histonet] Tissue Processor Advice

[caymanfl...@gmail.com]

We are in need of some advice regarding rapid tissue processors.  Models we
are considering:

Sakura Xpress
Leica Peloris
Thermo STP 420

It seems none of these models are perfect in every respect.  I'm interested
in anyone's opinions of these processors and your experience with them.

All input is appreciated!
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RE: [Histonet] Tissue Processor Advice

[Feher, Stephen]

We are using the Peloris with a 2 hr, 4 hr and 8 hr protocol.  We run 2
hour protocols throughout the day with an average of 4-5 runs per day
depending on specimen volume.  We really like this processor.  We have
had them for 10 months now, are using factory protocols and have not had
any specimens that have been either under or over processed.  The techs
and the pathologists are very pleased with it. 


Steve

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
caymanfl...@gmail.com
Sent: Friday, October 22, 2010 4:17 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Tissue Processor Advice

We are in need of some advice regarding rapid tissue processors.  Models
we are considering:

Sakura Xpress
Leica Peloris
Thermo STP 420

It seems none of these models are perfect in every respect.  I'm
interested in anyone's opinions of these processors and your experience
with them.

All input is appreciated!
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RE: [Histonet] Tissue Processor Advice

[Jesus Ellin]

How are you meeting the hours of fixation requirement for Breast?  With
2 and 4 and 8 hours,, But recently there are articles calling for Her 2
to be done on GI cases.  Just want to know you insight on this?

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Feher,
Stephen
Sent: Friday, October 22, 2010 2:12 PM
To: caymanfl...@gmail.com; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Tissue Processor Advice

We are using the Peloris with a 2 hr, 4 hr and 8 hr protocol.  We run 2
hour protocols throughout the day with an average of 4-5 runs per day
depending on specimen volume.  We really like this processor.  We have
had them for 10 months now, are using factory protocols and have not had
any specimens that have been either under or over processed.  The techs
and the pathologists are very pleased with it. 


Steve

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
caymanfl...@gmail.com
Sent: Friday, October 22, 2010 4:17 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Tissue Processor Advice

We are in need of some advice regarding rapid tissue processors.  Models
we are considering:

Sakura Xpress
Leica Peloris
Thermo STP 420

It seems none of these models are perfect in every respect.  I'm
interested in anyone's opinions of these processors and your experience
with them.

All input is appreciated!
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RE: [Histonet] Tissue Processor Advice

[WILLIAM DESALVO]

There are so many good to great processors on the market, but all have their 
plus and minus issues. You really have to decide what your two or three most 
important issues will be and then rank them. With the trend in becoming more 
efficient/cost effective, reducing TAT and LEAN process improvement, I suggest 
you look to improve your process to match these trends and you will be lead to 
rapid tissue processing in a LEAN way. Couple the previously mentioned 
trends/issues with versatility of processing with your routine formalin fixed 
samples with molecular fixed samples on the same instrument, I suggest the 
Sakura Xpress (X50 or X120) rapid processors.
 
These instruments provide continuous loading, small batch and require a small 
volume of reagent for processing and then discard. The instruments do have a 
required reagent kit and there is a variable pre-processing protocol, depending 
on the tissue type and fat content. Using the reagent kit does allow for cost 
savings over conventional processing and I find the pre-processing allows for 
better precision processing techniques and protocols, we have never over 
processed tissues. Another great advantage is the increased velocity of the 
workflow as the instruments are continuous load (no cleaning cycle between 
batches) and small batch (1 to 40 cassettes). Loading 1 or 2 cassettes when a 
STAT or RUSH cases arrives and completes fixation does not interrupt the 
process or require special handling. An important factor to consider is that 
continuous load processing does assist in workload leveling, which can assist 
in reducing employee stress, increase productivity and error reduction. All 
these things lead directly to reduced TAT. Add the often overlooked advantage 
of removing Xylene from your tissue processing, and again, I suggest you 
consider the Xpress.
 
I was an early adopter (5+ yrs use) and continue to use the X120 (2 units). I 
have not experienced any instrument performance or maintenance issues. I have 
had three software upgrades and the instruments had to go down for several 
hours to install the upgrade. The X120 and now the X50 have two programming 
options 1+ hour or 2+ hour processing. The most LEAN factor is that after the 
first basket of up to 40 cassettes, the next one comes off 20 or 40 minutes 
later and you can continuously load. There is no other instrument that can 
allow you to process in as small as batch or provide the continuous delivery of 
cassettes. You can do rapid processing with all of the instruments you are 
considering, but conventional, one reaction chamber instruments will limit the 
number of times the instrument can be run each day and that increases the batch 
size.
 
Rapid processing does demand change in the way your lab does it's work. The 
first is standardization of gross dissection to 3 mm thickness. It does not 
matter the instrument, if you want to truly move into rapid processing, then 
you have to standardize your process in the gross room. The process of 
retraining and standardizing your gross room is well worth the effort. Another 
issue that you have to fully consider is how will specific fixation times 
affect your workflow. We now have specific guidelines for ER/PgR; Her2 and if 
you follow the NCI protocols for cancer tumors, you have to record your actual 
fixation times. Trying to manage all the different fixation times becomes 
difficult and will slow down your process. If you use your tissue processor to 
complete fixation, the processor will force you into larger batches. When you 
have to wait for tissues to complete fixation before starting your processing 
program, you also limit the number of times the processor can be run in a day. 
I find that separating fixation from processing is the best approach. You 
process specimens only when the optimal fixation time has been completed. Stop 
and consider all the different tissue types, size, fat content and required 
protocols, and you will see the value of a rapid processor that has small batch 
and continuous load capabilities. Meet all your fixation requirements and needs 
and only use the tissue processor for processing, not fixation. This is very 
LEAN concept and a concept that I believe you will need to embrace.

Whenever you have the opportunity to change your process, I always suggest you 
look to improve the process, use the latest process improvement techniques and 
select an instrument that will assist in the change and prepare you for future 
change. My philosophy is we cannot continue to do tomorrow what we do today and 
expect a different outcome or result. Just my thoughts and experience, I hope 
this will help you. Do not hesitate to contact me if you have any questions.

William DeSalvo, B.S., HTL(ASCP)
 

 Date: Fri, 22 Oct 2010 16:16:50 -0400
 From: caymanfl...@gmail.com
 To: histonet@lists.utsouthwestern.edu
 Subject: [Histonet] Tissue Processor Advice
 
 We are in need of some advice regarding rapid tissue

[Histonet] tissue processor times

[Carol Bryant]

Hello everyone!
We have always had one program (catch-all) for all our specimens that we ran 
overnight on an Excelsior tissue processor.  We just purchased a new VIP 6 and 
I want to have a program for our larger fatty specimens such as breast cases 
and run our small biopsies on another program.  How much time do you use on 
each station for your larger cases such as breasts?  Particularly the formalin 
stations, 1 hour or 2 hours each?
Thanks in advance for your replies.


Carol Bryant, CT (ASCP)
Cytology/Histology Manager
Pathology Services
Lexington Clinic
Phone (859) 258-4082
Fax (859) 258-4081
cb...@lexclin.com



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Re: [Histonet] Tissue Processor Advice

[Anne van Binsbergen]

Sakura Tissue Tek VIP5!!
I have 2 of these sturdy workhorses and am very happy
Have been associated with VIP5's for as long as they have been around -
wonderful machines!
AnnieinAbuDhabi
aka.Annie out of Africa
2009/8/5 Igor Deyneko igor.deyn...@gmail.com

 Dear Histonetters!
 I need your advice in Tissue Processors. The one we currently use, Tissue
 Tek VIP 3000, is archaic and has finally died. So we are looking into
 buying
 a new one. I know that Thermo and Leica both have processors, as well as
 new
 Tissue teks, but I wanted to get opinions if you have a preference of a
 machine, pros and cons of each. I mostly process tumors, with occasional
 mouse organs thrown in.
 Any suggestions will be very helpful!
 Thank you in advance.
 Sincerely,
 Igor Deyneko
 Infinity Pharmaceuticals
 Cambridge, MA
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-- 
Anne van Binsbergen (Hope)
Abu Dhabi
UAE
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[Histonet] Tissue Processor Advice

[Igor Deyneko]

Dear Histonetters!
I need your advice in Tissue Processors. The one we currently use, Tissue
Tek VIP 3000, is archaic and has finally died. So we are looking into buying
a new one. I know that Thermo and Leica both have processors, as well as new
Tissue teks, but I wanted to get opinions if you have a preference of a
machine, pros and cons of each. I mostly process tumors, with occasional
mouse organs thrown in.
Any suggestions will be very helpful!
Thank you in advance.
Sincerely,
Igor Deyneko
Infinity Pharmaceuticals
Cambridge, MA
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Re: [Histonet] Tissue Processor Advice

[thecitan]

I recommend the new tissue tech.  I use it in my lab and its reliable and 
intuitive.

I strongly warn against TBS. I use one in my other lab and its been nothing but 
trouble. Its chemical storage is unreliable and leads to cross contamination.  
--Original Message--
From: Igor Deyneko
Sender: histonet-boun...@lists.utsouthwestern.edu
To: Histonet
Subject: [Histonet] Tissue Processor Advice
Sent: Aug 4, 2009 1:43 PM

Dear Histonetters!
I need your advice in Tissue Processors. The one we currently use, Tissue
Tek VIP 3000, is archaic and has finally died. So we are looking into buying
a new one. I know that Thermo and Leica both have processors, as well as new
Tissue teks, but I wanted to get opinions if you have a preference of a
machine, pros and cons of each. I mostly process tumors, with occasional
mouse organs thrown in.
Any suggestions will be very helpful!
Thank you in advance.
Sincerely,
Igor Deyneko
Infinity Pharmaceuticals
Cambridge, MA
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[Histonet] Tissue Processor

[Pathology]

FREE TBS Tissue Processor (you pay shipping).  Kind of a lemon.

Liesl Frasier
719-530-2200 x2195
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[Histonet] Tissue Processor

[Mark Tarango]

Does anyone happen to have a good used tissue processor for sale?  I'm also
interested in an embedding center and microtome.

Thanks

Mark
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