Hello all! If any licensed Histotechs are interested in working in the San
Francisco Bay area, Kaiser Permanente in Berkeley is hiring! Please send me
an email so I can assist on getting you on board!
On Sep 22, 2016 10:17 AM, <histonet-requ...@lists.utsouthwestern.edu> wrote:
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Today's Topics:
1. Positive Control for TRAP Staining (Jessica Riggleman)
2. CAP Inspection (Adesupo, Adesuyi (Banjo))
3. Unprocessing tissue from paraffin (Caroline Miller)
4. Mousse tissue processing (Blanca Lopez)
5. Quotes for capital (Record, Angela N)
6. Re: Mousse tissue processing (Caroline Miller)
7. Re: Unprocessing tissue from paraffin (Maria Mejia)
----------------------------------------------------------------------
Message: 1
Date: Wed, 21 Sep 2016 17:05:30 +0000
From: Jessica Riggleman <jriggle...@globusmedical.com>
To: "'histonet@lists.utsouthwestern.edu'
(histonet@lists.utsouthwestern.edu)"
<histonet@lists.utsouthwestern.edu>
Subject: [Histonet] Positive Control for TRAP Staining
Message-ID:
<D0AEE53F1F084340888BEB10C81DE9B295C5BB88@MBX2.gminc.local>
Content-Type: text/plain; charset="utf-8"
Hello,
Does anyone have a suggestion for a good positive control for TRAP staining?
Thank you,
Jessica Riggleman
_____________________________________________________________________
Jessica Riggleman | Research Associate
Globus Medical, Inc.
Valley Forge Business Center
2560 General Armistead Avenue | Audubon, PA 19403
Ph: (610) 930-1800 ext. 2583 | Fax:
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Message: 2
Date: Wed, 21 Sep 2016 12:54:06 -0500
From: "Adesupo, Adesuyi (Banjo)" <abades...@nrh-ok.com>
To: "'histonet@lists.utsouthwestern.edu'"
<histonet@lists.utsouthwestern.edu>
Subject: [Histonet] CAP Inspection
Message-ID:
<04ee4f75bb5fb246adb68d69b7460443b1006b6...@mail.nrhnt.nrh-ok.com>
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Hi,
We had our CAP Inspection last week. I had two Pathologists and a
Cytotechnologist (we don't do cytology) with me all day. There was no
Histotechnologist on the team.
I am just curious, whether any of you have had this type of inspection
before.
Best regards,
Banjo Adesuyi
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Message: 3
Date: Wed, 21 Sep 2016 12:38:00 -0700
From: Caroline Miller <mi...@3scan.com>
To: "Histonet@Lists. Edu" <histonet@lists.utsouthwestern.edu>
Subject: [Histonet] Unprocessing tissue from paraffin
Message-ID:
<cafthrqpasucgtfx-eo2dfyrjg_gbthn79pox9a-douz50es...@mail.gmail.com>
Content-Type: text/plain; charset=UTF-8
Hi there Histonetters!
I am currently working through some issues of unprocessing tissue to then
restain 'whole mount' with various stains, and then reprocess back to
paraffin.
I am running into issues of tissue being brittle and scratchy tissue after
reprocessing and sectioning.
Can anyone offer any advice on kinder dewaxing procedures and / or
additives to the unprocessing / processing fluids that might help in the
resulting piece of tissue?
I have currently been putting it through the cleaning cycle on the
processor, so standard xylene then alcohol treatment.
thanks in advance for your advice!
mills
--
Caroline Miller (mills)
Director of Histology
3Scan.com
415 2187297
------------------------------
Message: 4
Date: Wed, 21 Sep 2016 19:59:17 +0000
From: Blanca Lopez <blanca.lo...@utsouthwestern.edu>
To: "histonet@lists.utsouthwestern.edu"
<histonet@lists.utsouthwestern.edu>
Subject: [Histonet] Mousse tissue processing
Message-ID: <0d9b69779c50481c8c1be992255a8...@swms13mail12.swmed.org>
Content-Type: text/plain; charset="us-ascii"
Hello! Histonets.
Does anyone has a good protocol for processing mouse tissue? Somebody can
share.
thanks
Blanca Lopez
Histotech (ASCP)
UTSW Tissue Resource K1.210
Simmons Comprehensive Cancer Center
UT Southwestern Medical Center
Telephone: 214-648-7598
Email: blanca.lo...@utsouthwestern.edu
________________________________
UT Southwestern
Medical Center
The future of medicine, today.
------------------------------
Message: 5
Date: Wed, 21 Sep 2016 20:06:07 +0000
From: "Record, Angela N" <anrec...@saintfrancis.com>
To: "'histonet@lists.utsouthwestern.edu'"
<histonet@lists.utsouthwestern.edu>
Subject: [Histonet] Quotes for capital
Message-ID:
<20FC170D97CF6546939ABB0FA907C8DB039C70@SFHSEXCH001.WarrenNT.
SaintFrancis.Loc>
Content-Type: text/plain; charset="us-ascii"
Greg,
Could you please send me 2 separate budgetary quotes for a VIP6 and a
Prisma?
Thank You,
Angela Record, BS, HT(ASCP)
Saint Francis Hospital
Tulsa, OK
918-494-1321
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Message: 6
Date: Wed, 21 Sep 2016 15:37:03 -0700
From: Caroline Miller <mi...@3scan.com>
To: Blanca Lopez <blanca.lo...@utsouthwestern.edu>
Cc: "histonet@lists.utsouthwestern.edu"
<histonet@lists.utsouthwestern.edu>
Subject: Re: [Histonet] Mousse tissue processing
Message-ID:
<cafthrqoq3w6b+ac7p4f5-gsyzr_90joihs64lf_l30x9kzu...@mail.gmail.com>
Content-Type: text/plain; charset=UTF-8
Hi Blanca, This is what I use, note, no formalin on my machine, and you
could probably cut out one of the last alcohols
Step Routine tissue
70% alcohol 10
80% alcohol 10
90% alcohol 20
100% alcohol 20
100% alcohol 20
100% alcohol 30
100% alcohol 30
100% alcohol 30
Xylene 30
Xylene 35
paraffin 30
paraffin 30
paraffin 30
paraffin 30
paraffin at 60oC, P/V on, agit on
On Wed, Sep 21, 2016 at 12:59 PM, Blanca Lopez via Histonet <
histonet@lists.utsouthwestern.edu> wrote:
> Hello! Histonets.
> Does anyone has a good protocol for processing mouse tissue? Somebody can
> share.
> thanks
>
> Blanca Lopez
> Histotech (ASCP)
> UTSW Tissue Resource K1.210
> Simmons Comprehensive Cancer Center
> UT Southwestern Medical Center
> Telephone: 214-648-7598
> Email: blanca.lo...@utsouthwestern.edu
>
>
> ________________________________
>
> UT Southwestern
>
>
> Medical Center
>
>
>
> The future of medicine, today.
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
--
Caroline Miller (mills)
Director of Histology
3Scan.com
415 2187297
------------------------------
Message: 7
Date: Wed, 21 Sep 2016 19:10:06 -0700
From: Maria Mejia <mbmph...@gmail.com>
To: Caroline Miller <mi...@3scan.com>
Cc: "Histonet@Lists. Edu" <histonet@lists.utsouthwestern.edu>
Subject: Re: [Histonet] Unprocessing tissue from paraffin
Message-ID: <a430919d-af5e-4474-84e7-541844cda...@gmail.com>
Content-Type: text/plain; charset=utf-8
Hi Millis,
Lets see if I?m understanding you correctly.
1) You have unprocessed tissue. What kind of unprocessed tissue & how thick
are the free-floating sections?
2) Are the tissue fixed? If so, with what? & For how long?
3) You want to stain the unknown whole mount using various histochemical
stains or IHC stains?
4) Can you please explain why after staining you need to reprocess for
paraffin?
Cause once these mystery sections are processed you'll have to cut them
using a rotary microtome.
I can maybe help you, but your going to have to provide more detail
information. Also provide your
processing protocol.
Regards
Maria Mejia
Lead Histologist
Memory & Aging Center
UCSF
> On Sep 21, 2016, at 12:38 PM, Caroline Miller via Histonet <
histonet@lists.utsouthwestern.edu> wrote:
>
> Hi there Histonetters!
>
> I am currently working through some issues of unprocessing tissue to then
> restain 'whole mount' with various stains, and then reprocess back to
> paraffin.
>
> I am running into issues of tissue being brittle and scratchy tissue after
> reprocessing and sectioning.
>
> Can anyone offer any advice on kinder dewaxing procedures and / or
> additives to the unprocessing / processing fluids that might help in the
> resulting piece of tissue?
>
> I have currently been putting it through the cleaning cycle on the
> processor, so standard xylene then alcohol treatment.
>
> thanks in advance for your advice!
> mills
>
>
>
>
> --
> Caroline Miller (mills)
> Director of Histology
> 3Scan.com
> 415 2187297
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
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