Zack Daugherty <zackdaughe...@gmail.com> ha scritto:
Hello all,
This is my first time wading into geometric mophometry as a PhD candidate.
First off the project:
I plan to track geometric morphometry data of Nassau grouper we have raised
in the lab. There are some 130 juveniles that are now capable of being
anesthetized and handled. I plan to gather data down to the individual
level for this cohort monthly to establish data on individual ontogenetic
trajectories. But I have some questions regarding 2D capture, because *once
I start this there is no going back*. The welfare of these animals
supercedes the morphometry work I have planned (these fish will be used for
future broodstock at our aquaculture facility).
[cut]
Questions:
Any other protocols that people are aware of I should look at for digital
capture?
Dear Zack,
if I was you, I would also think about the advantages/disadvantages of
anesthesia. I have used it in certain situations but, depending on
what your downstream application and target speies are, sometimes one
can get away with not anestesizing fish (certain species, especially
when raised in captive conditions, even let you pin them to extend
their fins). Considering that you stated that welfare of the fish is
particularly important for you, that's the first thing that came to
mind.
Also, this is probably very obvious, pay attention to keeping the fish
straight (i.e. limit dorso-ventral arching). This kind of artifact
will still probably present in your data, but it's good to try
limiting it at its source. Your fish species is not extremely
elongated so it's probably not going to be a very strong problem.
Should I surround my specimens in black material?
In my opinion, probably not if they are mostly grey as the specimen in
the picture...
Should I purchase any white balance card sets?
I guess it depends how important is color to you...
Would a grid backdrop be neccessary for every photo behind the specimen or
just to initially calibrate a field a view before photographing specimens?
In my opinion, the most important part is having some sort of scale in
the picture, possibly not too far from the specimen.
Well, that's my two cents, I hope this helps...
Best,
Carmelo
--
Carmelo Fruciano
Honorary Fellow - University of Catania - Catania, Italy
e-mail c.fruci...@unict.it
http://www.fruciano.it/research/
--
MORPHMET may be accessed via its webpage at http://www.morphometrics.org
To unsubscribe from this group and stop receiving emails from it, send an email
to morphmet+unsubscr...@morphometrics.org.
