Re: [R] interpolation using R for PCR quantification

2015-12-28 Thread Jeff Newmiller
There is some terminology confusion here... interpolation as implemented by approx or spline usually means estimating values between known points. You seem to have approximate (not known) points, and are looking to apply a linear regression model to estimate missing data. Beware that mixing

[R] interpolation using R for PCR quantification

2015-12-24 Thread Luigi Marongiu
Dear all, I am a newbie in interpolation using R and I would like to learn better the procedure. I am applying interpolation to quantify nucleic acid targets using an assay known as PCR. To do this, I have two sets of variables: standard of known concentrations and query for which I need to