[Samtools-help] phased vcf from phased Bam

2017-04-20 Thread Shilpa Garg
Hi, I phase read alignments using `samtools phase`. How can I get the phased VCF from phased BAM files? Is it possible to do at the command line? -- Check out the vibrant tech community on one of the world's most engaging

Re: [Samtools-help] problem with samtools mpileup

2017-04-20 Thread Federico Abascal
I asked this sometime ago… The reason is because when you provide the fasta reference file, then BAQ is enabled. BAQ does realignment and base quality score recalibration and results in different mpileup outputs. In my experience BAQ is very good at removing false positives, but when the

Re: [Samtools-help] problem with samtools mpileup

2017-04-20 Thread James Bonfield
On Thu, Apr 20, 2017 at 03:52:11PM +0900, kenichi chiba wrote: > I would like to ask for help about samtools mpileup. The output of > samtools mpileup may differ depending on the presence or absence of the > -f option. If there are probably about 20 bp deletions, some deletions > are missing

[Samtools-help] problem with samtools mpileup

2017-04-20 Thread kenichi chiba
Hello, I would like to ask for help about samtools mpileup. The output of samtools mpileup may differ depending on the presence or absence of the -f option. If there are probably about 20 bp deletions, some deletions are missing from the output. $ samtools --version samtools 1.2 Using htslib