Hi John,

The target is for the quality of the software to improve with
subsequent releases.  Do you notice that you have more incorrect
(decoy) hits with higher probability?  BTW, the latest version is 4.4
not 4.3.  For more information you can check the release notes
http://tools.proteomecenter.org/wiki/index.php?search=release+notes&go=Go

-David

On Tue, Sep 14, 2010 at 7:45 AM, John Damask <jbdam...@gmail.com> wrote:
> Hi,
> We recently upgraded an old version of TPP (Squall v3.3) to v4.3 rev1.
> We've noticed an increase in the number of high confidence  (<1% FDR)
> peptides and proteins. Digging into the pepXml files we've seen cases
> where, for example, a peptide previously assigned a probability of < .
> 5 now scores > .9. Note that the Mascot scores haven't changed
>
> Where can I learn about the differences between versions of the
> Prophets?
> Thanks
> John
>
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