Dear Sir/Madam,

I have a query related to PTMprophet analysis.


I performed comet search for an mzml file and put it for further analysis
as under:



-Using TPP tools Analyse peptides Checked for iprophet proteinprophet
PTMprophet


-Mass added for PTMs were:


- 79.966 Da for Phosphorylation- STY



I filtered the data on the basis of error table values.


- I exported the excel showing results as under.


[image: image.png]


  *Query: Everything is fine except that the position of modified amino
acid is not there, only the mass is there and amino acid is highlighted. *



*How can I get to know the modified site (I mean exact position of modified
amino acid?*


*Is there a way that I can get these modified sites with respect to
protein. e.g. S27 or T82 etc. *



Could you please help me for this.


Thanks and regards,

*Mehar Un Nissa*
Research Scholar,
Proteomics Lab.
Department of Biosciences and Bioengineering,
Indian Institute of Technology, Bombay-400076

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