To add a comment: the phenomenon happens for a few of the most abundant
proteins, having many peptides. The peptide abundances look OK in the
quantitation.tsv file, so I may be able to work around this by
recalculating the ratios from this file.
Magnus
On Wednesday, 8 August 2018 15:18:33 UTC+2, magnus....@gmail.com wrote:
>
> Dear TPP Team,
>
> I am processing some TMT-10 data, and observed a strange behavior for some
> proteins (see example). Although I am getting good quantitative information
> on the peptide level for many peptides, no quantitative information is
> reported on the protein level for this and a few other proteins. The
> peptides with all zeroes have no TMT-signal in the MS3 spectrum despite a
> strong ID from the MS2, so they are correct. Other proteins with some
> peptides having all zeroes from Libra do produce protein-level Libra
> results in the same analysis. the LIBRA9 and LIBRA10 channels are negative
> controls, and should be empty (or at least much lower than LIBRA1-8).
> Overall, the results agree well with MaxQuant, so I don't think it is a
> major or systematic issue at all.
>
> In the quantitation.tsv, many of the peptides with non-zero quantitation
> are kept ("Yes" in the "kept?" column), but the protein ratios
> (protratio0-9) are still all zero!
>
> I used default settings in PeptideProphet and ProteinProphet, except for
> selecting a TMT-10 conditions file for Libra (with SRS data turned on) in
> TPP v5.1.0 Syzygy, Build 201711031215-7670 (Windows_NT-x86_64).
>
> Any ideas?
>
>
> Cheers,
>
> Magnus
>
>
>
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