> From: "Jeffrey La Favre" <[email protected]>
> To: "Silver List" <[email protected]>
> Subject: Correction to formula for resolution of light microscope
> Date: Wed, 29 Jul 1998 23:31:35 -0400
> Reply-to: [email protected]
Hi Jeff,
If I got this right, a good light microscope will "see" down to 0.2
microns, and, would a darkfield "see" even better? Maybe down to 0.1
micron or less? Sounds like were getting close to seeing what we need
to see!
Thanks for the math!
Jim
> In double checking my message I discovered an error in the resolution
> formula. The correct formula is given below:
>
> R = wavelength/ (2)(numerical aperture)
>
> Then the resolution would be:
>
> R = 0.5 microns/ (2)(1.3) = 0.2 microns
>
> Therefore, the smallest particle that can be measured directly by a good
> light microscope is about 0.2 microns in diameter.
>
> Sorry for the error in my first message. Hope I have it correct this time!
>
> Jeff La Favre
>
>
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