Andy and 3Wishes,

Thank you for posting this work.

I had my dear wife, the microbiologist, read this over my shoulder. 
She came up with one thing you should do to *complete* the experiment 
properly.

You have a positive control, in that you prove that your culture is 
viable by inoculating a plate with it.

But you also need the negative control as follows:

In addition to putting a drop of culture in 1:2 ... 1:n dilutions, 
you must *also* put a drop of culture in water that contains *no* 
silver, wait the same three minutes, and do the agar plate just as 
with the other samples.

This will eliminate the possibility that the bugs are merely being 
killed by diluting them in pure water. Unlikely, I know, but an 
inevitable question that will arise. 

Both a positive and negative control are part of a well designed
experiment.

Excellent job!

Be well,

Mike D.

> Hello All
> 
> This article was sent to me by a person that is doing some research
> with CS. He has asked that his name not be mentioned until a latter
> date.  I don't know any more than appears in this post.   I hope
> more will follow.
> 
> Andy
>   -----Original Message-----
>   From: [email protected] [mailto:[email protected]]
>   Sent: Wednesday, May 26, 1999 10:06 AM To: Andrew Sloop Subject:
>   Re: Technical Papers
> 
>       A friend handed me a generator asking what I thought about it
>       and
> secondly, because I had just read a comment by U.S. Justice Dept..
> Attorney-investigator John Loftus about a substance called Movidyn
> which the Russians found being produced in the satellite state of
> Czechoslovakia. They were more than a little concerned and removed
> the plant because Movidyn was found to be an effective antidote
> against every microorganism in their bio-warfare arsenal ! Then,
> Loftus said Movidyn was a powder form of colloidal silver !
> 
>        Since pure drinkable water may become a life and death issue
>        in this
> country within the next 12 months, I became very interested. My
> investigations have only proven half of what I'd like to know but I
> am convinced that colloidal silver is definitely an amazing
> substance. I'll share what I have determined so far.
> 
>       Firstly, the pure Coll.. Ag should be generated slowly in pure
> distilled water (no salt or other additive) with a 27, or
> preferably, 36 volt source, and with .999 pure silver electrodes.
> Carried to a self limited end point ( where electrode oxide forms
> too rapidly to continue generation), a strong solution is produced
> which is colorless and crystal clear at the time. But after 12-24
> hours (store in a dark place), the solution accomplishes what is
> called colloid dispersion whereon it turns a beautiful yellow-gold
> to amber-gold color- the deeper, the higher the colloid content.
> Still the solution is crystal clear. Now, at the time of generation,
> or after dispersion, you can appraise yourself of the presence and
> concentration (relatively) of the colloidal silver content via the
> 'Tyndal effect' method. This is best done with one of these laser
> pen lights. In a dark room you direct the light beam through the
> solution (in a glass container). The colloid particles will cause
> the appearance of a distinct shaft of white light through the
> solution, sometimes as distinct as a shaft of wood. (Pure water will
> give no such effect at all.)
> 
>       In my work, I have not had the advantage of quantitative '
>       parts per
> million' analysis of the solutions I have generated.(I understand
> that such is a little expensive.) So far, I have bent my
> investigation toward anti-bacterial studies. In these studies, I
> have used CDC standard pathogenic organisms- Staph.aureus, beta
> hemolytic streptococcus pyogenes, Psuedomonas aeruginosa, Proteus
> mirabilis and Bacillus subtillus (spore former), and Escherichia
> coli. Test suspensions of these organisms for testing have been BHI
> broth type at concentration of approximately one million organisms
> per cc. In other words, so far I have tested with an overburden of
> bacterial concentration. Shortly, I will test for effect with
> lightly contaminated specimens.(as I can find time!)
> 
>        So- my delightful findings so far are these- with a strongly
> generated Coll.Ag. Sol. used against very strong suspension of each
> of the above mentioned organisms:
>      If I inoculate 2 cc. Ag. Sol. with one drop (50,000 organisms),
>      I get a
> total kill in less than 3 minutes. (total kill determined by
> subculture of the Ag. and organism mixture to a Blood Agar plate and
> incubating overnight); then I can take the original Coll. Ag.
> solution and make dilutions in distilled water at 1:2, 1:4, 1:8,
> 1:16, 1:32, 1:64 etc.
>     Then, when I inoculate these dilutions with the above pathogens
>     I can
> get a total kill of all six types all the way to the 1:32 dilution
> with an exposure time of 3 minutes! That has been very impressive to
> me. I realize that I now need work of a finer nature but I first
> wanted to prove the raw kill power of Coll.. Ag. for myself. I think
> that a 1:32 dil.. that will kill pathogens at 50,000 org. per drop
> in less than 3 minutes is really something.( And when I say kill I
> mean there is not one colony on the Blood Agar plates, and I always
> run a control plate on each organism suspension which demonstrates
> the viability and count.)
> 
> 
>    I am convinced that with lighter, more realistic
>   concentration of
> the organism and more than 3 min. reaction time, much greater
> dilution of the Coll.. Ag Sol.. will
> 
> 
[Mike Devour, Citizen, Patriot, Libertarian]
[[email protected]                       ]
[Speaking only for myself...              ]


--
The silver-list is a moderated forum for discussion of colloidal silver.

To join or quit silver-list or silver-digest send an e-mail message to: 
[email protected]  -or-  [email protected]
with the word subscribe or unsubscribe in the SUBJECT line.

To post, address your message to: [email protected]

List maintainer: Mike Devour <[email protected]>